SSGCID: Target Page

AnphA.17081.n

P44-79 outer membrane protein

CENTER ID:	AnphA.17081.n
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17081.n.A2.GE30755	Mature protein( AnphA.17081.n )		30	461

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1448
RefSeq:	YP_505855.1
UniProt:	Q2GIE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKALSYGKLF WRVCVILSLA MLMTGNSAYA EQDSKISSHS NSKRDGYFYV GLDYSPTFVN IKNFSIGESS GGTKGIYPYL RDGDVVKLEA GRFDWNRPDP WIGFDDNMFV TMGGSAGYGM GNTRIEVEIG YERFKTKGTK GSRDTEDYAD TVYLLAKDLA YNVASGKASN LAVALAGVSG KEIVGFADSL KEKHPGIDRQ ICKRVKGALR RGLLQDDEKA GNCDTGVGTH TVVIKKTEGA QQQQRISLQP YSGPLGLRAS LGGRGVEKWP RINNRHSGHV YSSSFLKKAG IVQKDSSGYS YQRDASGTVA NDIKELYSEA REIVAKLFLT AIEGAEVIEI KGISSTSFIV NVCSDIVADD FVAVPYACLG LGGNIIEFVE GRSDLRPAYK LKVGLSYQLS QKVNAFADVF YHRVLGNGEY SDLPVRHLVD DHSPEGRSKN AATANFSMKY TGAELGIQLR F

NT Sequence

ATGAAGGCTC TAAGTTATGG CAAGCTGTTT TGGCGAGTAT GTGTGATCTT GTCGTTAGCT ATGCTCATGA CAGGTAATAG TGCATATGCC GAACAGGATT CTAAAATTAG TTCTCACTCT AATAGCAAAA GGGATGGTTA TTTCTACGTG GGGTTAGATT ACAGCCCTAC ATTTGTGAAT ATAAAAAATT TTAGTATAGG AGAAAGTAGC GGGGGAACTA AAGGTATATA CCCATACCTG AGGGACGGAG ATGTAGTCAA GCTGGAAGCT GGCAGATTTG ATTGGAATAG ACCTGATCCT TGGATTGGTT TTGATGACAA CATGTTTGTT ACCATGGGCG GAAGCGCTGG GTATGGCATG GGGAACACCA GAATAGAAGT AGAGATTGGT TACGAGCGCT TCAAGACTAA GGGTACTAAA GGTAGTAGAG ATACAGAGGA TTACGCTGAT ACAGTGTATT TATTAGCTAA AGATTTAGCA TATAATGTCG CCTCAGGAAA GGCTAGCAAT CTCGCGGTAG CCCTCGCCGG GGTTTCTGGA AAGGAAATAG TTGGTTTTGC TGATTCTCTA AAGGAAAAGC ATCCTGGAAT TGATAGGCAA ATTTGTAAGA GAGTGAAAGG CGCTTTGCGC AGAGGGCTGC TACAAGACGA CGAAAAAGCA GGAAATTGTG ATACTGGGGT GGGAACTCAT ACGGTAGTGA TAAAAAAAAC AGAAGGCGCA CAACAACAGC AAAGAATATC ACTACAGCCA TATAGCGGGC CTTTAGGCCT TAGAGCTTCT TTAGGTGGTA GAGGCGTTGA GAAATGGCCG CGCATTAACA ATCGTCATTC TGGTCATGTG TACTCAAGTT CCTTCCTAAA AAAAGCAGGG ATTGTACAAA AGGACAGCAG CGGATATAGC TATCAACGAG ATGCTTCAGG TACAGTTGCT AATGACATAA AAGAACTTTA CAGCGAAGCT AGAGAGATAG TTGCTAAACT ATTTCTGACA GCTATTGAAG GTGCAGAAGT TATAGAAATA AAAGGCATTT CTTCGACTTC CTTCATTGTT AATGTGTGCT CAGATATCGT TGCTGACGAC TTTGTAGCAG TTCCATATGC TTGCTTGGGT TTAGGAGGCA ACATAATTGA ATTTGTAGAG GGGCGTAGTG ACCTTAGACC CGCATACAAG CTGAAAGTGG GCCTAAGTTA TCAGCTTTCA CAGAAAGTTA ATGCATTTGC TGATGTATTC TACCATAGAG TTTTAGGTAA TGGGGAGTAC TCGGATTTAC CAGTAAGACA CTTGGTTGAT GATCACAGCC CCGAGGGAAG ATCAAAAAAC GCAGCCACTG CTAATTTTAG CATGAAATAT ACTGGCGCAG AACTTGGCAT ACAACTAAGG TTT

Details for AnphA.17081.n.A2.GE30755

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	70

Validated AA Sequence

Validated NT Sequence

anattttgtt tanctttaag aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg ccgaacagga ttctaaaatt agttctcact ctaatagcaa aagggatggt tatttctacg tggggttaga ttacagccct acatttgtga atataaaaaa ttttagtata ggagaaagta gcgggggaac taaaggtata tacccatacc tgagggacgg agatgtagtc aagctggaag ctggcagatt tgattggaat agacctgatc cttggattgg ttttgatgac aacatgtttg ttaccatggg cggaagcgct gggtatggca tggggaacac cagaatagaa gtagagattg gttacgagcg cttcaagact aagggtacta aaggtagtag agatacagag gattacgctg atacagtgta tttattagct aaagatttag catataatgt cgcctcagga aaggctagca atctcgcggt agccctcgcc ggggtttctg gaaaggaaat agttggtttt gctgattctc taaaggaaaa gcatcctgga attgataggc aaatttgtaa gagagtgaaa ggcgctttgc gcagagggct gctacaagac gacgaaaaag caggaaattg tgatactggg gtgggaactc atacggtagt gataaaaaaa acagaaggcg cacaacaaca gcaaagaata tcactacagc catatagcgg gcctttaggc cttanagctt ctttaggtgg tagaggcgtt gagaaatggc cgcgcattaa caatcgtcat tctggtcatg tgtactcaag ttccttccta aaaaaagctg tacaaaagga cagcancgga tatagctatc ancgagatgc ttcnggtaca gttgctaatg acatnaaana actttacagc gaagctagag agatactaaa ntatttctga cngctatgnn gnngcagnnn ntagaantna nnnattnnnn acttcnnntn nnnnngngnt nnnanntcnt nctgangant tgnacantcn nnngcnnnnt nnntnnnnng nnnnnnnnnn nngtngacnt nnnnnnnntn nnnnnnnnan nnnnnnnnnn nnntnannnn c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAEQDSKIS SHSNSKRDGY FYVGLDYSPT FVNIKNFSIG ESSGGTKGIY PYLRDGDVVK LEAGRFDWNR PDPWIGFDDN MFVTMGGSAG YGMGNTRIEV EIGYERFKTK GTKGSRDTED YADTVYLLAK DLAYNVASGK ASNLAVALAG VSGKEIVGFA DSLKEKHPGI DRQICKRVKG ALRRGLLQDD EKAGNCDTGV GTHTVVIKKT EGAQQQQRIS LQPYSGPLGL RASLGGRGVE KWPRINNRHS GHVYSSSFLK KAGIVQKDSS GYSYQRDASG TVANDIKELY SEAREIVAKL FLTAIEGAEV IEIKGISSTS FIVNVCSDIV ADDFVAVPYA CLGLGGNIIE FVEGRSDLRP AYKLKVGLSY QLSQKVNAFA DVFYHRVLGN GEYSDLPVRH LVDDHSPEGR SKNAATANFS MKYTGAELGI QLRF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgaacag gattctaaaa ttagttctca ctctaatagc aaaagggatg gttatttcta cgtggggtta gattacagcc ctacatttgt gaatataaaa aattttagta taggagaaag tagcggggga actaaaggta tatacccata cctgagggac ggagatgtag tcaagctgga agctggcaga tttgattgga atagacctga tccttggatt ggttttgatg acaacatgtt tgttaccatg ggcggaagcg ctgggtatgg catggggaac accagaatag aagtagagat tggttacgag cgcttcaaga ctaagggtac taaaggtagt agagatacag aggattacgc tgatacagtg tatttattag ctaaagattt agcatataat gtcgcctcag gaaaggctag caatctcgcg gtagccctcg ccggggtttc tggaaaggaa atagttggtt ttgctgattc tctaaaggaa aagcatcctg gaattgatag gcaaatttgt aagagagtga aaggcgcttt gcgcagaggg ctgctacaag acgacgaaaa agcaggaaat tgtgatactg gggtgggaac tcatacggta gtgataaaaa aaacagaagg cgcacaacaa cagcaaagaa tatcactaca gccatatagc gggcctttag gccttagagc ttctttaggt ggtagaggcg ttgagaaatg gccgcgcatt aacaatcgtc attctggtca tgtgtactca agttccttcc taaaaaaagc agggattgta caaaaggaca gcagcggata tagctatcaa cgagatgctt caggtacagt tgctaatgac ataaaagaac tttacagcga agctagagag atagttgcta aactatttct gacagctatt gaaggtgcag aagttataga aataaaaggc atttcttcga cttccttcat tgttaatgtg tgctcagata tcgttgctga cgactttgta gcagttccat atgcttgctt gggtttagga ggcaacataa ttgaatttgt agaggggcgt agtgacctta gacccgcata caagctgaaa gtgggcctaa gttatcagct ttcacagaaa gttaatgcat ttgctgatgt attctaccat agagttttag gtaatgggga gtactcggat ttaccagtaa gacacttggt tgatgatcac agccccgagg gaagatcaaa aaacgcagcc actgctaatt ttagcatgaa atatactggc gcagaacttg gcatacaact aaggttttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa