AnphA.17081.k

P44-14 outer membrane protein

CENTER ID: AnphA.17081.k
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17081.k.A2.GE30752 Mature protein( AnphA.17081.k ) 15 433
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1305
RefSeq: YP_505746.1
UniProt: Q2GIQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMSMAIVMAG SDVRAHDDVS ALDTGGAGYF YVGLDYSPAF SKIRDFSIRE SNGETKAVYP YLKDGKSVKL ESHKFDWNTP DPRIGFKDNM LVAMEGSVGY GIGGARVELE IGYERFKTKG IRDSGSKEDE ADTVYLLAKE LAYDVVTGQT DKLTAALAKT SGKDIVQFAK TLGISHSEID KKVCSGSHAK GNSETTKNNT VASYTEKPTA ATATAQCSGF PDEAAGNKAG GLNRFVSETK VEEGKNWPTG KIHSGSSTNN VDGTHNGNAK AVATDLVNLN RDEKTIVAGL LAKTIEGGEV VEIRAVSSTS VMVNACYDLL SEGLGVVPYA CVGLGGNFVG VVDGHITPKL AYRLKAGLSY QLSPEISAFA GGFYHRVVGD GVYDDLPAQR LVDDTSPAGR TKDTAIANFS MAYVGASFQP YYAVLLVELY EYR
NT Sequence
ATGATGTCGA TGGCTATAGT CATGGCTGGG AGTGATGTTA GGGCTCATGA TGACGTTAGC GCTTTGGATA CTGGTGGTGC GGGATATTTC TATGTTGGTT TGGATTACAG TCCAGCGTTT AGCAAGATAA GAGATTTTAG TATAAGGGAG AGTAACGGAG AGACTAAGGC AGTATATCCA TACTTAAAGG ATGGAAAGAG TGTAAAGCTT GAGTCACACA AGTTTGACTG GAACACTCCT GATCCTCGGA TTGGGTTTAA GGACAACATG CTTGTAGCTA TGGAAGGTAG TGTTGGTTAT GGTATTGGTG GTGCCAGGGT TGAGCTTGAG ATTGGTTACG AGCGCTTCAA GACCAAGGGT ATTAGAGATA GTGGTAGTAA GGAAGATGAA GCTGATACAG TATATCTACT AGCTAAGGAG TTAGCTTATG ATGTTGTTAC TGGACAAACT GATAAACTTA CTGCTGCTCT TGCTAAGACC TCTGGGAAGG ACATCGTTCA GTTTGCTAAG ACTCTTGGAA TTTCTCATTC CGAGATTGAT AAGAAGGTTT GTAGTGGGAG CCATGCGAAG GGGAATAGTG AGACAACAAA AAATAATACT GTAGCTAGCT ATACTGAAAA GCCAACAGCG GCAACAGCAA CGGCACAATG TAGTGGTTTT CCTGATGAGG CTGCAGGAAA CAAAGCAGGG GGATTGAATA GGTTTGTAAG CGAAACGAAA GTGGAAGAAG GTAAAAACTG GCCTACGGGC AAGATACACA GTGGGAGCAG CACTAACAAC GTGGATGGCA CACACAATGG AAACGCCAAA GCCGTAGCCA CAGACCTAGT AAATCTTAAT CGTGACGAAA AAACCATAGT AGCAGGGTTA TTAGCTAAAA CTATTGAAGG GGGTGAGGTT GTAGAAATTA GGGCCGTTTC TTCTACTTCT GTGATGGTCA ATGCTTGCTA TGATCTTCTT AGTGAAGGTC TAGGCGTTGT TCCTTATGCT TGTGTCGGTT TAGGTGGTAA CTTCGTGGGC GTTGTTGATG GGCATATCAC TCCTAAGCTT GCTTATAGAT TAAAGGCTGG CTTGAGTTAT CAGCTCTCTC CTGAAATCTC GGCTTTTGCT GGGGGTTTCT ACCATCGTGT TGTGGGAGAT GGTGTCTATG ATGATCTGCC GGCTCAACGT CTTGTAGATG ATACTAGTCC GGCGGGCCGT ACTAAGGATA CTGCTATTGC TAACTTCTCC ATGGCCTATG TTGGTGCATC ATTTCAGCCA TATTACGCAG TTCTTCTAGT AGAACTGTAT GAGTATCGA
Details for AnphA.17081.k.A2.GE30752
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 47kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 37
PERCENT COVERAGE: 16
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMXXXXXXXX XXGGXRXXXX XXXXXXXXXX SXXXXAXXXX XXXELXAXTX XX
Validated NT Sequence
attttnttta nctttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgnnn nnnnnngncn tnnnnnnggn nngnnctggt ggtgngagat gntgntnnnt aaannngnnn nacannnncn gcnnnnnnnn ntcnnagnat ncngntgctn gcanagnccn nnannnnnnt gagttggntg cngncaccnn tnnnnnntaa ctnnnnnnaa gcttgagtca cacaagtttg actggaacac tcctgatcct cggattgggt ttaaggacaa catgcttgta gctatggaag gtagtgttgg ttatggtatt ggtggtgcca gggttgagct tgagattggt tacgagcgct tcaagaccaa gggtattaga gatagtggta gtaaggaaga tgaagctgat acagtatatc tactagctaa ggagttagct tatgatgttg ttactggaca aactgataaa cttactgctg ctcttgctaa gacctctggg aaggacatcg ttcagtttgc taagactctt ggaatttctc attccgagat tgataagaag gtttgtagtg ggagccatgc gaaggggaat agtgagacaa caaaaaataa tactgtagct agctatactg aaaagccaac agcggcaaca gcaacggcac aatgtagtgg ttttcctgat gaggctgcag gaaacaaagc anggggattg aataggtttg taagcgaaac gaaagtggaa gaaggtaaaa actggcctac gggcnagana cacagtgnga gcagcactaa caacgtggat ggcacacaca atggaaacgc caaagccgta gccncngacc tagtaaatct taatcgtgac gaaaaaccat agtagcnnnn tattagctaa aactattnna nngggtgang ntgtagaaat tnnggncgtt tcnnctactn cngtgatggn caatgctngc tatgatcnnc ttnntgnnnn ctnnngttgn tnnatgctnn nnnnnnntnn nngnnannnn nnnnncntnn nnntggnnnt atcanncnan ntnnnnnnnn nnnnnnnngn nntgnnntnn nnncnnnntn nnnnnnnnnn nnttnnnnnn nnnnnnnnnn nnnnnnnnnn nannnnnnnn annnnnnnnn nnanng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHDDVSAL DTGGAGYFYV GLDYSPAFSK IRDFSIRESN GETKAVYPYL KDGKSVKLES HKFDWNTPDP RIGFKDNMLV AMEGSVGYGI GGARVELEIG YERFKTKGIR DSGSKEDEAD TVYLLAKELA YDVVTGQTDK LTAALAKTSG KDIVQFAKTL GISHSEIDKK VCSGSHAKGN SETTKNNTVA SYTEKPTAAT ATAQCSGFPD EAAGNKAGGL NRFVSETKVE EGKNWPTGKI HSGSSTNNVD GTHNGNAKAV ATDLVNLNRD EKTIVAGLLA KTIEGGEVVE IRAVSSTSVM VNACYDLLSE GLGVVPYACV GLGGNFVGVV DGHITPKLAY RLKAGLSYQL SPEISAFAGG FYHRVVGDGV YDDLPAQRLV DDTSPAGRTK DTAIANFSMA YVGASFQPYY AVLLVELYEY R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctcatgat gacgttagcg ctttggatac tggtggtgcg ggatatttct atgttggttt ggattacagt ccagcgttta gcaagataag agattttagt ataagggaga gtaacggaga gactaaggca gtatatccat acttaaagga tggaaagagt gtaaagcttg agtcacacaa gtttgactgg aacactcctg atcctcggat tgggtttaag gacaacatgc ttgtagctat ggaaggtagt gttggttatg gtattggtgg tgccagggtt gagcttgaga ttggttacga gcgcttcaag accaagggta ttagagatag tggtagtaag gaagatgaag ctgatacagt atatctacta gctaaggagt tagcttatga tgttgttact ggacaaactg ataaacttac tgctgctctt gctaagacct ctgggaagga catcgttcag tttgctaaga ctcttggaat ttctcattcc gagattgata agaaggtttg tagtgggagc catgcgaagg ggaatagtga gacaacaaaa aataatactg tagctagcta tactgaaaag ccaacagcgg caacagcaac ggcacaatgt agtggttttc ctgatgaggc tgcaggaaac aaagcagggg gattgaatag gtttgtaagc gaaacgaaag tggaagaagg taaaaactgg cctacgggca agatacacag tgggagcagc actaacaacg tggatggcac acacaatgga aacgccaaag ccgtagccac agacctagta aatcttaatc gtgacgaaaa aaccatagta gcagggttat tagctaaaac tattgaaggg ggtgaggttg tagaaattag ggccgtttct tctacttctg tgatggtcaa tgcttgctat gatcttctta gtgaaggtct aggcgttgtt ccttatgctt gtgtcggttt aggtggtaac ttcgtgggcg ttgttgatgg gcatatcact cctaagcttg cttatagatt aaaggctggc ttgagttatc agctctctcc tgaaatctcg gcttttgctg ggggtttcta ccatcgtgtt gtgggagatg gtgtctatga tgatctgccg gctcaacgtc ttgtagatga tactagtccg gcgggccgta ctaaggatac tgctattgct aacttctcca tggcctatgt tggtgcatca tttcagccat attacgcagt tcttctagta gaactgtatg agtatcgata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a