AnphA.17081.j

P44-72 outer membrane protein

CENTER ID: AnphA.17081.j
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17081.j.A2.GE30751 Mature protein( AnphA.17081.j ) 29 435
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.715
RefSeq: YP_505246.1
UniProt: Q2GK55

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAKNYNNLF LGSIMMSLAV LITCQGAQAR DAAKSTFEVE KPQYFYIGLD YSPAFSNIRN FTIQESNGET KAIYPYIRGD KEIILDASRF DWNMPNPRIG FKNSTLVAVG SSIGYAMGSM RLEIEVSHER FKSRGRYIGN EREEESDTIY LLAKELGHST VTEKTEDLVS ALTKVKGEDI VRLANALEKT RPDIGRKICR TRSITGGKNY GKYNRYTSWL KDTNNLTALC GDIGGNSDKH EHHNMIQNSF KDFAREALLG GSTNWPTTTG DKTRGNDNAK ALARDLITVL SREEKIAVAG LMVNTIEGGE VVDIGEISST SVMVNVCYDF LSKEGSIVPY GCMGLGSNFV GIVKGHFTPK PAYRLKAGVS YKLFSGVDIF AAGFYHRVFG STEYTDIPVE YLIDDVSPYG RTKETAMADF RMSYAGGEFG MRFAF
NT Sequence
ATGAGCGCTA AAAATTATAA TAACCTATTT TTAGGAAGCA TAATGATGTC TCTTGCCGTG CTAATAACTT GTCAAGGAGC TCAAGCAAGG GATGCTGCGA AAAGTACTTT TGAGGTTGAG AAACCCCAGT ATTTCTATAT AGGATTGGAT TACAGTCCAG CATTTAGCAA CATAAGAAAC TTCACTATAC AGGAAAGCAA CGGTGAAACT AAGGCGATAT ATCCGTACAT TAGGGGAGAT AAGGAGATAA TACTGGATGC TAGTAGATTT GACTGGAATA TGCCAAACCC GCGCATAGGC TTTAAAAACA GTACGCTTGT AGCTGTAGGA AGTAGTATTG GTTATGCTAT GGGCAGTATG AGACTTGAAA TTGAAGTTTC TCATGAGCGT TTTAAGAGCC GGGGGAGATA TATTGGAAAT GAAAGAGAAG AAGAATCTGA TACAATATAT CTCCTTGCGA AGGAGTTAGG ACATAGCACA GTCACTGAAA AAACCGAAGA TCTTGTATCA GCTTTGACAA AAGTAAAAGG TGAAGATATT GTGCGCCTCG CTAATGCCTT AGAGAAGACC CGTCCAGATA TTGGTAGAAA GATCTGTAGG ACTCGCAGTA TTACTGGAGG TAAAAACTAC GGTAAGTATA ATAGATACAC TTCGTGGCTC AAAGATACTA ATAATCTCAC AGCCTTATGT GGAGATATAG GAGGTAACAG CGATAAACAT GAACATCACA ACATGATTCA AAATTCTTTT AAGGACTTCG CGCGTGAAGC TCTGCTAGGT GGTAGCACAA ACTGGCCAAC AACAACTGGT GATAAGACAC GGGGAAACGA CAATGCAAAG GCATTAGCAA GAGACCTAAT AACAGTCTTA AGCCGAGAAG AAAAGATAGC AGTTGCGGGA TTAATGGTAA ATACTATTGA AGGGGGTGAA GTAGTAGACA TTGGTGAAAT TTCTTCTACC TCCGTAATGG TAAATGTGTG TTACGATTTC TTGAGTAAAG AAGGATCTAT AGTTCCCTAT GGATGCATGG GATTAGGCAG TAATTTTGTG GGCATTGTCA AGGGACACTT TACTCCAAAG CCAGCTTATA GATTAAAGGC TGGTGTCAGT TATAAGCTTT TCTCTGGTGT TGATATCTTT GCGGCAGGGT TCTACCATAG GGTTTTTGGT AGCACCGAAT ATACAGACAT TCCTGTAGAG TACTTGATAG ATGATGTGAG TCCTTACGGA CGTACTAAGG AGACTGCAAT GGCTGATTTC AGAATGAGTT ATGCTGGGGG TGAGTTTGGT ATGAGATTTG CTTTT
Details for AnphA.17081.j.A2.GE30751
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 32
Validated AA Sequence
MPNPRIGFKN STLVAVGSSI GYAMGSMRLE IEVSHERFKS RGRYIGNERE EESDTIYLLA KELGHSTVTE KTEDLVSALT KVKGEDIVRL ANALRRPVQI LVERSVGLAV LLEVKTTVSI IDTLRGSKIL IISQPYVEI
Validated NT Sequence
ttntttnntt nangaaggng annnnnnnng gnncnncncc nncnccatcn tatggntncc cnggaagcnc nacccnnggn cctggttcga tggcaaggga tgctgcgaaa agtanttttg aggttgagaa accccactat ttctatatan gattggatta cngnccanca tttaccaaca taagaaactt cactatacag gaaagcaacg gtgaaactan gnnatntatc cgtacnttag gggagatnag gagataatac tggatgctag tagatttgac tggaatatgc cnaacccgcg cataggcttt aaaaacagta cgcttgtagc tgtaggaagt agtattggtt atgctatggg cagtatgaga cttgaaattg aagtttctca tgagcgtttt aagagccggg ggagatatat tggaaatgaa agagaagaag aatctgatac aatatatctc cttgcgaagg agttaggaca tagcacagtc actgaaaaaa ccgaagatct tgtatcagct ttgacaaaag taaaaggtga agatattgtg cgcctcgcta atgccttaag aagacccgtc cagatattgg tagaaagatc tgtaggactc gcagtattac tggaggtaaa aactacggta agtataatag atacacttcg tggctcaaag atactaataa tctcacagcc ttatgtggag atataggagg taacagcgat aaacatgaac atcacaacat gattcaaaan tcttttaagg acttcgcgcg tgaagctctg ctaggtggta gcacaaactg gncancaacn actggtgatn aganacnggn naacgacaat gnnaaggcat tagcnagana nnnaataann gtctnnancc nanaanaaaa gatancantt gcngnatnan nggtaaatac tatngaangg ggtgaagtan tagacattgn tgnaatttct tctaccnccn taatggnaaa ngtgtgntan nnntncntnn antnnnnaan gnnctantag ntnccntatn gnatgcangn nnnnnncagt aanttttnnn ggnnntngnn nnngnnncnc nttnnnnnna nnnncnnnnn tnnnnantna nnnnnnnnnn nagnntnnnn nnnnnnnnnn nnnnnnnntn nnngnnnnnn nnnntnnnnn nnncnnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMARDAAKST FEVEKPQYFY IGLDYSPAFS NIRNFTIQES NGETKAIYPY IRGDKEIILD ASRFDWNMPN PRIGFKNSTL VAVGSSIGYA MGSMRLEIEV SHERFKSRGR YIGNEREEES DTIYLLAKEL GHSTVTEKTE DLVSALTKVK GEDIVRLANA LEKTRPDIGR KICRTRSITG GKNYGKYNRY TSWLKDTNNL TALCGDIGGN SDKHEHHNMI QNSFKDFARE ALLGGSTNWP TTTGDKTRGN DNAKALARDL ITVLSREEKI AVAGLMVNTI EGGEVVDIGE ISSTSVMVNV CYDFLSKEGS IVPYGCMGLG SNFVGIVKGH FTPKPAYRLK AGVSYKLFSG VDIFAAGFYH RVFGSTEYTD IPVEYLIDDV SPYGRTKETA MADFRMSYAG GEFGMRFAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaagggat gctgcgaaaa gtacttttga ggttgagaaa ccccagtatt tctatatagg attggattac agtccagcat ttagcaacat aagaaacttc actatacagg aaagcaacgg tgaaactaag gcgatatatc cgtacattag gggagataag gagataatac tggatgctag tagatttgac tggaatatgc caaacccgcg cataggcttt aaaaacagta cgcttgtagc tgtaggaagt agtattggtt atgctatggg cagtatgaga cttgaaattg aagtttctca tgagcgtttt aagagccggg ggagatatat tggaaatgaa agagaagaag aatctgatac aatatatctc cttgcgaagg agttaggaca tagcacagtc actgaaaaaa ccgaagatct tgtatcagct ttgacaaaag taaaaggtga agatattgtg cgcctcgcta atgccttaga gaagacccgt ccagatattg gtagaaagat ctgtaggact cgcagtatta ctggaggtaa aaactacggt aagtataata gatacacttc gtggctcaaa gatactaata atctcacagc cttatgtgga gatataggag gtaacagcga taaacatgaa catcacaaca tgattcaaaa ttcttttaag gacttcgcgc gtgaagctct gctaggtggt agcacaaact ggccaacaac aactggtgat aagacacggg gaaacgacaa tgcaaaggca ttagcaagag acctaataac agtcttaagc cgagaagaaa agatagcagt tgcgggatta atggtaaata ctattgaagg gggtgaagta gtagacattg gtgaaatttc ttctacctcc gtaatggtaa atgtgtgtta cgatttcttg agtaaagaag gatctatagt tccctatgga tgcatgggat taggcagtaa ttttgtgggc attgtcaagg gacactttac tccaaagcca gcttatagat taaaggctgg tgtcagttat aagcttttct ctggtgttga tatctttgcg gcagggttct accatagggt ttttggtagc accgaatata cagacattcc tgtagagtac ttgatagatg atgtgagtcc ttacggacgt actaaggaga ctgcaatggc tgatttcaga atgagttatg ctgggggtga gtttggtatg agatttgctt tttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa