AnphA.17081.i

P44-18ES, P44 outer membrane protein expression locus with P44-18

CENTER ID: AnphA.17081.i
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.17081.i.A2.GE30750 Mature protein( AnphA.17081.i ) 26 431
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1312
RefSeq: YP_505752.1
UniProt: Q2GIP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKGKIILGS VMMSMAIVMA GNDVRAHDDV SALETGGAGY FYVGLDYSPA FSKIRDFSIR ESNGETKAVY PYLKDGKSVK LESHKFDWNT PDPRIGFKDN MLVAMEGSVG YGIGGARVEL EIGYERFKTK GIRDSGSKED EADTVYLLAK ELAYDVVTGQ TDNLAAALAK TSGKDIVQFA KAVEISNSGI GKKVCETKRK DGDTTNRFAK YIVGAGDSSN AGTSLCGGKN QKSSDTDTGV EKAQALHDFV SNTLSDGTKN WPTSSETSKS NNDNAKAVAG DLTKKLTPEE KTIVAGLLAK TIEGGEVVEI RAVSSTSVMV NACYDLLSEG LGVVPYACVG LGGNFVGVVD GHITPKLAYR LKAGLSYQLS PEISAFAGGF YHRVVGDGVY DDLPAQRLVD DTSPAGRTKD TAIANFSMAY VGGEFGVRFA F
NT Sequence
ATGAGAAAAG GAAAGATAAT CTTAGGAAGC GTAATGATGT CGATGGCTAT AGTCATGGCT GGGAATGATG TCAGGGCTCA TGATGACGTT AGCGCTTTGG AGACTGGTGG TGCGGGATAT TTCTATGTTG GTTTGGATTA CAGTCCAGCG TTTAGCAAGA TAAGAGATTT TAGTATAAGG GAGAGTAACG GAGAGACTAA GGCAGTATAT CCATACTTAA AGGATGGAAA GAGTGTAAAG CTAGAGTCAC ACAAGTTTGA CTGGAACACA CCTGATCCTC GGATTGGGTT TAAGGACAAC ATGCTTGTAG CTATGGAAGG CAGTGTTGGG TACGGTATTG GTGGTGCCAG GGTTGAGCTT GAGATTGGTT ACGAGCGCTT CAAGACCAAG GGTATTAGAG ATAGTGGTAG TAAGGAAGAT GAAGCAGATA CAGTATATCT ACTAGCTAAG GAGTTAGCTT ATGATGTTGT TACTGGGCAG ACTGATAACC TTGCCGCTGC TCTTGCCAAA ACCTCAGGTA AGGACATCGT TCAGTTTGCT AAGGCCGTGG AGATTTCTAA TTCCGGTATT GGTAAGAAGG TGTGTGAGAC AAAGCGGAAG GATGGTGATA CTACGAACAG GTTTGCAAAG TATATAGTTG GTGCGGGTGA TAGTAGCAAT GCTGGTACAT CATTGTGTGG TGGTAAGAAC CAAAAGAGTT CGGACACAGA CACCGGGGTG GAGAAGGCTC AGGCTCTGCA TGACTTTGTT TCTAACACAT TGAGTGATGG TACTAAGAAC TGGCCTACGT CGAGTGAAAC GTCTAAATCG AATAACGACA ACGCCAAAGC TGTAGCGGGA GACTTAACTA AGAAGCTCAC CCCTGAAGAA AAAACCATAG TAGCAGGGTT ACTAGCTAAG ACTATTGAAG GTGGTGAGGT TGTTGAAATT AGGGCGGTTT CTTCTACTTC TGTGATGGTT AATGCTTGTT ATGATCTTCT TAGTGAAGGC TTAGGTGTCG TTCCTTATGC TTGCGTCGGT CTTGGTGGTA ACTTCGTGGG CGTTGTTGAT GGGCATATCA CTCCTAAGCT TGCTTATAGA TTAAAGGCTG GGTTGAGTTA TCAGCTCTCT CCTGAAATCT CGGCTTTTGC TGGGGGTTTC TACCATCGTG TTGTGGGAGA TGGTGTTTAT GATGATCTGC CGGCTCAACG TCTTGTAGAT GATACTAGTC CGGCGGGTCG TACTAAGGAT ACTGCTATTG CTAACTTCTC CATGGCTTAT GTCGGTGGGG AATTTGGTGT TAGGTTTGCT TTT
Details for AnphA.17081.i.A2.GE30750
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 45kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAHDDVSAL ETGGAGYFYV GLDYSPAFSK IRDFSIRESN GETKAVYPYL KDGKSVKLES HKFDWNTPDP RIGFKDNMLV AMEGSVGYGI GGARVELEIG YERFKTKGIR DSGSKEDEAD TVYLLAKELA YDVVTGQTDN LAAALAKTSG KDIVQFAKAV EISNSGIGKK VCETKRKDGD TTNRFAKYIV GAGDSSNAGT SLCGGENQKG SDTDTGVEKA QALHDFVSNT LSDGTKNWPT SSETSKSNND NAXAVAGDLT XKLTPEEKTI VAXXLAXTIX XGEVVEIXAX XXYFCDG
Validated NT Sequence
attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctc atgatgacgt tagcgctttg gagactggtg gtgcgggata tttctatgtt ggtttggatt acagtccagc gtttagcaag ataagagatt ttagtataag ggagagtaac ggagagacta aggcagtata tccatactta aaggatggaa agagtgtaaa gctagagtca cacaagtttg actggaacac acctgatcct cggattgggt ttaaggacaa catgcttgta gctatggaag gcagtgttgg gtacggtatt ggtggtgcca gggttgagct tgagattggt tacgagcgct tcaagaccaa gggtattaga gatagtggta gtaaggaaga tgaagcagat acagtatatc tactagctaa ggagttagct tatgatgttg ttactgggca gactgataac cttgccgctg ctcttgccaa aacctcaggt aaggacatcg ttcagtttgc taaggccgtg gagatttcta attccggtat tggtaagaag gtgtgtgaga caaagcggaa ggatggtgat actacgaaca ggtttgcaaa gtatatagtt ggtgcgggtg atagtagcaa tgctggtaca tcattgtgtg gtggtgagaa tcaaaagggt tcggacacag acaccggggt ggagaaggct caggctctgc atgactttgt ttctaacaca ttgagtgatg gtactaagaa ctggcctacg tcgagtgaaa cgtctaaatc gaataacgac aacgccnaag ctgtagcggg agacttaact aanaagctca cccctgaaga aaaaaccata gtagcnngnt nactagctaa nactattgan nnnggtgagg ttgttgaaat tnnggcggnn tncntctact tctgtgatgg ntaatgcttg nnangatctt ctnnngnagn cnnnnngncn tnccnnatgc ntgcntnnnt cntnnnnnaa ctnnnngggn cnnnnnangg natatcnctn cnanctncnn nnnatnannn nnnnnnnnnn nannnnncnc nnannnnnnn nnnnngnnnn nnnnnnnnnn nnggnnannn nnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHDDVSAL ETGGAGYFYV GLDYSPAFSK IRDFSIRESN GETKAVYPYL KDGKSVKLES HKFDWNTPDP RIGFKDNMLV AMEGSVGYGI GGARVELEIG YERFKTKGIR DSGSKEDEAD TVYLLAKELA YDVVTGQTDN LAAALAKTSG KDIVQFAKAV EISNSGIGKK VCETKRKDGD TTNRFAKYIV GAGDSSNAGT SLCGGKNQKS SDTDTGVEKA QALHDFVSNT LSDGTKNWPT SSETSKSNND NAKAVAGDLT KKLTPEEKTI VAGLLAKTIE GGEVVEIRAV SSTSVMVNAC YDLLSEGLGV VPYACVGLGG NFVGVVDGHI TPKLAYRLKA GLSYQLSPEI SAFAGGFYHR VVGDGVYDDL PAQRLVDDTS PAGRTKDTAI ANFSMAYVGG EFGVRFAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctcatgat gacgttagcg ctttggagac tggtggtgcg ggatatttct atgttggttt ggattacagt ccagcgttta gcaagataag agattttagt ataagggaga gtaacggaga gactaaggca gtatatccat acttaaagga tggaaagagt gtaaagctag agtcacacaa gtttgactgg aacacacctg atcctcggat tgggtttaag gacaacatgc ttgtagctat ggaaggcagt gttgggtacg gtattggtgg tgccagggtt gagcttgaga ttggttacga gcgcttcaag accaagggta ttagagatag tggtagtaag gaagatgaag cagatacagt atatctacta gctaaggagt tagcttatga tgttgttact gggcagactg ataaccttgc cgctgctctt gccaaaacct caggtaagga catcgttcag tttgctaagg ccgtggagat ttctaattcc ggtattggta agaaggtgtg tgagacaaag cggaaggatg gtgatactac gaacaggttt gcaaagtata tagttggtgc gggtgatagt agcaatgctg gtacatcatt gtgtggtggt aagaaccaaa agagttcgga cacagacacc ggggtggaga aggctcaggc tctgcatgac tttgtttcta acacattgag tgatggtact aagaactggc ctacgtcgag tgaaacgtct aaatcgaata acgacaacgc caaagctgta gcgggagact taactaagaa gctcacccct gaagaaaaaa ccatagtagc agggttacta gctaagacta ttgaaggtgg tgaggttgtt gaaattaggg cggtttcttc tacttctgtg atggttaatg cttgttatga tcttcttagt gaaggcttag gtgtcgttcc ttatgcttgc gtcggtcttg gtggtaactt cgtgggcgtt gttgatgggc atatcactcc taagcttgct tatagattaa aggctgggtt gagttatcag ctctctcctg aaatctcggc ttttgctggg ggtttctacc atcgtgttgt gggagatggt gtttatgatg atctgccggc tcaacgtctt gtagatgata ctagtccggc gggtcgtact aaggatactg ctattgctaa cttctccatg gcttatgtcg gtggggaatt tggtgttagg tttgcttttt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa