SSGCID: Target Page

AnphA.17081.h

P44-16 outer membrane protein

CENTER ID:	AnphA.17081.h
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.17081.h.A1.GE30749	Full length( AnphA.17081.h )		1	429

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1360
RefSeq:	YP_505788.1
UniProt:	Q2GIL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMSMAIVMAG NDVRAHDDVS ALETGGAGYF YVGLDYSPAF SKIRDFSIRE SNGETKAVYP YLKDGKSVKL ESHKFDWNTP DPRIGFKDNM LVAMEGSVGY GIGGARVELE IGYERFKTKG IRDSGSKEDE ADTVYLLAKE LAYDVVTGQT DNLAAALAKT SGKDIAQFAN AVKITNSTID GKVCSGKHAA LVTGKGKTYH EKPGTTEDNS AQCSGLGDGS AGADQKFLSK FVSAVGLSEN KNWPTGQAGK STGGPEVGAP NSNANAVAKD LVALNRDEKT IVAGLLTKTI EGGEVVEIRA VSSTSVMVNA CYDLLSEGLG VVPYACVGLG GNFVGVVDGH ITPKLAYRLK AGLSYQLSPE ISAFAGGFYH RVVGDGVYDD LPAQRLVDDT SPAGRTKDTA IANGFYHRVV GDDVYESRGI KIVGVQHHA

NT Sequence

ATGATGTCTA TGGCTATAGT CATGGCTGGG AATGATGTTA GGGCTCATGA TGACGTTAGC GCTTTGGAAA CTGGTGGTGC GGGATATTTC TATGTTGGTT TGGATTACAG TCCAGCGTTT AGCAAGATAA GAGATTTTAG TATAAGGGAG AGTAACGGAG AGACTAAGGC AGTATATCCA TACTTAAAGG ATGGAAAGAG TGTAAAGCTA GAGTCACACA AGTTTGACTG GAACACTCCT GATCCTCGGA TTGGGTTTAA GGACAACATG CTTGTAGCTA TGGAAGGCAG TGTTGGTTAT GGTATTGGTG GTGCCAGGGT TGAGCTTGAG ATTGGTTACG AGCGCTTCAA GACCAAGGGT ATTAGAGATA GTGGTAGTAA GGAAGATGAA GCTGATACAG TATATCTACT AGCTAAGGAG TTAGCTTATG ATGTTGTTAC TGGGCAGACT GATAACCTTG CTGCTGCTCT TGCCAAGACC TCTGGGAAGG ACATAGCACA ATTTGCGAAT GCTGTGAAAA TTACCAACTC CACTATCGAT GGAAAGGTTT GTAGTGGAAA ACATGCAGCG TTGGTAACTG GTAAAGGAAA GACGTATCAT GAAAAACCGG GCACAACTGA AGACAATTCT GCGCAATGTA GCGGGTTAGG CGACGGTAGC GCGGGAGCAG ACCAAAAGTT TTTGAGTAAG TTTGTGAGTG CTGTAGGGTT GTCGGAGAAT AAAAACTGGC CTACTGGTCA GGCGGGAAAG AGCACGGGCG GTCCAGAGGT TGGCGCGCCT AATAGCAACG CCAACGCCGT AGCTAAAGAC CTAGTAGCTC TTAATCGTGA CGAAAAAACC ATAGTAGCAG GGTTACTAAC TAAAACTATT GAAGGTGGTG AGGTTGTTGA GATCAGGGCG GTTTCTTCTA CTTCTGTGAT GGTTAATGCT TGTTATGATC TTCTTAGTGA AGGTTTAGGC GTTGTTCCTT ATGCTTGTGT CGGTCTCGGT GGGAACTTCG TGGGCGTTGT TGATGGGCAT ATCACTCCTA AGCTTGCTTA TAGGTTAAAG GCTGGGTTGA GTTATCAGCT ATCTCCTGAA ATCTCCGCTT TTGCTGGGGG ATTCTATCAT CGCGTTGTGG GAGATGGTGT CTATGATGAT CTGCCGGCTC AACGTCTTGT AGATGATACT AGTCCGGCGG GTCGTACTAA GGATACTGCT ATTGCTAACG GTTTCTACCA TCGCGTTGTT GGTGATGATG TTTATGAATC TAGGGGAATC AAAATAGTAG GAGTGCAACA TCATGCA

Details for AnphA.17081.h.A1.GE30749

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	47kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	43

Validated AA Sequence

MLVAMEGSVG YGIGGARVEL EIGYERFKTK GIRDSGSKED EADTVYLLAK ELAYDVVTGQ TDNLAAALAK TSGKDIAQFA NAVKITNSTI DGKVCSGKHA ALVTGKGKTY HEKPGTTEDN SAQCSGLXDG SAXADQKFLS KFVSAVGLSE NKNWPTGXAG KSTGGPXVGA PNSNANAVXK DLVXLNRDEK TIVAXX

Validated NT Sequence

ttnnnnnact ttcnannntn nntgccntgn nnntctcnat nancnngntg ngngtacnnn gnannnnnna nnangctgnn ncnnngangn annngnnnnn ncanntggct gggaatgatg ttagggctca tgatgacgtt agcgctttgg aaactggtgg tgcgggatat ttctatgttg gtttggatta cagtccagcg tttagcaaga taagagattt tagtataagg gagagtaacg gagagactaa ggcagtatat ccatacttaa aggatggaaa gagtgtaaag ctagagtcac acaagtttga ctggaacact cctgatcctc ggattgggtt taaggacaac atgcttgtag ctatggaagg cagtgttggt tatggtattg gtggtgccag ggttgagctt gagattggtt acgagcgctt caagaccaag ggtattagag atagtggtag taaggaagat gaagctgata cagtatatct actagctaag gagttagctt atgatgttgt tactgggcag actgataacc ttgctgctgc tcttgccaag acctctggga aggacatagc acaatttgcg aatgctgtga aaattaccaa ctccactatc gatggaaagg tttgtagtgg aaaacatgca gcgttggtaa ctggtaaagg aaagacgtat catgaaaaac cgggcacaac tgaagacaat tctgcgcaat gtagcgggtt angcgacggt agcgcangag cagaccaaaa gtttttgagt aagtttgtga gtgctgtagg gttgtcggag aataaaaact ggcctactgg tcangcggga aagagcacgg gcggtccaga ngttggcgcg cctaatagca acgccaacgc cgtanctaaa gacctagtan ctcttaatcg tgacgaaaaa accatagtag cnnnnnacta actaaaacta ttgnangngg tgaggntgnt ganatcnngc ngtttcttct acttctgtga tgnnnnngcn tgnntgatgn annntnnnnn tnnnctnngc tnnnnnngnc tngnnggant nnnggtnnnn ngnnnnntcn nnnnanctgc tnnngntnag nnnnnngacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncnncnnnnn nnnngaannn nggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMMSMAIVMA GNDVRAHDDV SALETGGAGY FYVGLDYSPA FSKIRDFSIR ESNGETKAVY PYLKDGKSVK LESHKFDWNT PDPRIGFKDN MLVAMEGSVG YGIGGARVEL EIGYERFKTK GIRDSGSKED EADTVYLLAK ELAYDVVTGQ TDNLAAALAK TSGKDIAQFA NAVKITNSTI DGKVCSGKHA ALVTGKGKTY HEKPGTTEDN SAQCSGLGDG SAGADQKFLS KFVSAVGLSE NKNWPTGQAG KSTGGPEVGA PNSNANAVAK DLVALNRDEK TIVAGLLTKT IEGGEVVEIR AVSSTSVMVN ACYDLLSEGL GVVPYACVGL GGNFVGVVDG HITPKLAYRL KAGLSYQLSP EISAFAGGFY HRVVGDGVYD DLPAQRLVDD TSPAGRTKDT AIANGFYHRV VGDDVYESRG IKIVGVQHHA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgtctatg gctatagtca tggctgggaa tgatgttagg gctcatgatg acgttagcgc tttggaaact ggtggtgcgg gatatttcta tgttggtttg gattacagtc cagcgtttag caagataaga gattttagta taagggagag taacggagag actaaggcag tatatccata cttaaaggat ggaaagagtg taaagctaga gtcacacaag tttgactgga acactcctga tcctcggatt gggtttaagg acaacatgct tgtagctatg gaaggcagtg ttggttatgg tattggtggt gccagggttg agcttgagat tggttacgag cgcttcaaga ccaagggtat tagagatagt ggtagtaagg aagatgaagc tgatacagta tatctactag ctaaggagtt agcttatgat gttgttactg ggcagactga taaccttgct gctgctcttg ccaagacctc tgggaaggac atagcacaat ttgcgaatgc tgtgaaaatt accaactcca ctatcgatgg aaaggtttgt agtggaaaac atgcagcgtt ggtaactggt aaaggaaaga cgtatcatga aaaaccgggc acaactgaag acaattctgc gcaatgtagc gggttaggcg acggtagcgc gggagcagac caaaagtttt tgagtaagtt tgtgagtgct gtagggttgt cggagaataa aaactggcct actggtcagg cgggaaagag cacgggcggt ccagaggttg gcgcgcctaa tagcaacgcc aacgccgtag ctaaagacct agtagctctt aatcgtgacg aaaaaaccat agtagcaggg ttactaacta aaactattga aggtggtgag gttgttgaga tcagggcggt ttcttctact tctgtgatgg ttaatgcttg ttatgatctt cttagtgaag gtttaggcgt tgttccttat gcttgtgtcg gtctcggtgg gaacttcgtg ggcgttgttg atgggcatat cactcctaag cttgcttata ggttaaaggc tgggttgagt tatcagctat ctcctgaaat ctccgctttt gctgggggat tctatcatcg cgttgtggga gatggtgtct atgatgatct gccggctcaa cgtcttgtag atgatactag tccggcgggt cgtactaagg atactgctat tgctaacggt ttctaccatc gcgttgttgg tgatgatgtt tatgaatcta ggggaatcaa aatagtagga gtgcaacatc atgcataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa