AnphA.11339.a

metallo-beta-lactamase family protein

CENTER ID:	AnphA.11339.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.11339.a.A2.GE27629	Mature protein( AnphA.11339.a )		19	263

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_129512
PATRIC ID:	fig\|212042.8.peg.1137
RefSeq:	YP_505618.1
UniProt:	Q2GJ33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKITILGCGS SVGVPSVGCE CGVCASEHPH NKRTRTSALI EKGSTRLLID ASPDLRIQAL RQNFYTMDAV LFTHCHADHT TGISELQAFT PKVGYHIPVY SDFGTLALIT ASNAYLFIPS TPGAPWKKCH YLTVNPVRHN VEFNIGEFSI MAIKQSHGEV NSNGFIFDNS VAYCTDVKSF PEASWERLRN KHMIIFGCLR YQAVGAHAHV DLCIEWIREL KPHIAVFTHM SHDIDYEQLL DYLHSKLPGQ KVVVAHDGLE LEL

NT Sequence

ATGAAAATTA CGATTCTTGG ATGTGGGTCG TCAGTAGGTG TTCCATCGGT CGGGTGTGAG TGCGGGGTGT GTGCCTCGGA ACATCCGCAT AATAAGAGAA CAAGAACGTC TGCGCTCATA GAGAAGGGGA GTACGCGCTT GTTAATTGAT GCGTCTCCAG ATTTGCGCAT CCAAGCGCTG CGTCAAAACT TTTACACCAT GGATGCGGTT TTATTTACTC ATTGTCATGC AGATCACACA ACAGGAATTT CCGAGCTGCA GGCATTTACC CCAAAGGTTG GTTACCATAT TCCAGTCTAT AGCGATTTTG GAACTTTGGC ATTGATCACG GCCAGTAACG CTTATCTTTT CATACCCAGT ACGCCAGGGG CTCCATGGAA AAAGTGCCAT TACTTAACAG TGAATCCAGT TCGCCATAAT GTTGAGTTCA ATATCGGTGA GTTCAGTATC ATGGCGATTA AGCAATCTCA CGGTGAGGTA AACAGTAATG GGTTCATATT TGATAATTCT GTAGCATATT GTACAGATGT AAAATCGTTT CCTGAAGCAT CATGGGAGCG CTTGCGTAAT AAGCATATGA TTATCTTCGG GTGTCTCAGA TATCAGGCAG TGGGTGCACA TGCTCATGTG GACCTATGTA TAGAATGGAT TAGGGAATTG AAGCCGCATA TAGCTGTCTT TACTCACATG AGCCATGACA TAGATTATGA GCAACTTTTA GATTACTTGC ATAGTAAATT GCCGGGGCAA AAAGTTGTTG TAGCACACGA TGGCTTGGAG CTGGAGCTG

Details for AnphA.11339.a.A2.GE27629

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMCECGVCAS EHPHNKRTRT SALIEKGSTR LLIDASPDLR IQALRQNFYT MDAVLFTHCH ADHTTGISEL QAFTPKVGYH IPVYSDFGTL ALITASNAYL FIPSTPGAPW KKCHYLTVNP VRHNVEFNIG EFSIMAIKQS HGEVNSNGFI FDNSVAYCTD VKSFPEASWE RLRNKHMIIF GCLRYQAVGA HAHVDLCIEW IRELKPHIAV FTHMSHDIDY EQLLDYLHSK LPGQKVVVAH DGLEL

Validated NT Sequence

ccagctccaa gccatcgtgt gctacaacaa ctttttgccc cggcaattta ctatgcaagt aatctaaaag ttgctcataa tctatgtcat ggctcatgtg agtaaagaca gctatatgcg gcttcaattc cctaatccat tctatacata ggtccacatg agcatgtgca cccactgcct gatatctgag acacccgaag ataatcatat gcttattacg caagcgctcc catgatgctt caggaaacga ttttacatct gtacaatatg ctacagaatt atcaaatatg aacccattac tgtttacctc accgtgagat tgcttaatcg ccatgatact gaactcaccg atattgaact caacattatg gcgaactgga ttcactgtta agtaatggca ctttttccat ggagcccctg gcgtactggg tatgaaaaga taagcgttac tggccgtgat caatgccaaa gttccaaaat cgctatagac tggaatatgg taaccaacct ttggggtaaa tgcctgcagc tcggaaattc ctgttgtgtg atctgcatga caatgagtaa ataaaaccgc atccatggtg taaaagtttt gacgcagcgc ttggatgcgc aaatctggag acgcatcaat taacaagcgc gtactcccct tctctatgag cgcagacgtt cttgttctct tattatgcgg atgttccgag gcacacaccc cgcactcaca catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgtgagtgc ggggtgtgtg cctcggaaca tccgcataat aagagaacaa gaacgtctgc gctcatagag aaggggagta cgcgcttgtt aattgatgcg tctccagatt tgcgcatcca agcgctgcgt caaaactttt acaccatgga tgcggtttta tttactcatt gtcatgcaga tcacacaaca ggaatttccg agctgcaggc atttacccca aaggttggtt accatattcc agtctatagc gattttggaa ctttggcatt gatcacggcc agtaacgctt atcttttcat acccagtacg ccaggggctc catggaaaaa gtgccattac ttaacagtga atccagttcg ccataatgtt gagttcaata tcggtgagtt cagtatcatg gcgattaagc aatctcacgg tgaggtaaac agtaatgggt tcatatttga taattctgta gcatattgta cagatgtaaa atcgtttcct gaagcatcat gggagcgctt gcgtaataag catatgatta tcttcgggtg tctcagatat caggcagtgg gtgcacatgc tcatgtggac ctatgtatag aatggattag ggaattgaag ccgcatatag ctgtctttac tcacatgagc catgacatag attatgagca acttttagat tacttgcata gtaaattgcc ggggcaaaaa gttgttgtag cacacgatgg cttggagctg gagctgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa