SSGCID: Target Page

AnphA.10201.a

methionyl-tRNA synthetase

CENTER ID:	AnphA.10201.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.10201.a.A1.GE27627	Full length( AnphA.10201.a )		1	513

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.10201.a.A1.PW27984	Full length( AnphA.10201.a )		1	513

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1157
RefSeq:	YP_505630.1
UniProt:	Q2GJ21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSKHFCVTT PIYYVNDVPH IGHFYTTLIS DVLARFKRLD GDNVKFTTGT DEHGQKIEET ARKRGTPVLQ FVDEVSESFR ELVHKSGFCA DDFIRTTESR HKDAVVALWD RLYSRGQIYK GSYAGFYSVR DETFYQERDL IDGKAPTGAE VTWLEEPGYF FKLSEWQEAL LQLYQDNPRF VVPEGKLSEV ISFVKSGLRD LSVSRSKAHL SWGIGVPGDP EHLIYVWVDA LSNYLALVGF PNEDAEDYNN FWCGENSHVV HVVGKDILKF HAVYWPALLM AAGLPLPKQV VAHGWWLNEG QKISKSLGNV ICPLTIINEY GLDNVRYFLL SETPIGNDAS FSAKRLKERV NCDLANNIGN LVQRTVTLVH KWCEGKVPSV DMKLMCGDEA LPDYQGILEQ YRVYVEERRF FEALKFIIGV SSTANEYIAV KAPWKLFKED TALAEAVLFK LLEYIKCIGL LLQPFLPVTS EKLLEQIAVP VEKRKFEHIA ELCTGRVLPK AVPIFSKFDM DDV

NT Sequence

ATGTCAAGTA AGCACTTCTG TGTTACCACG CCCATATATT ATGTGAACGA TGTTCCCCAC ATTGGGCACT TTTATACCAC GCTTATATCA GATGTTCTTG CAAGGTTTAA GCGTCTGGAT GGTGATAATG TAAAGTTTAC TACCGGAACA GATGAGCATG GCCAGAAAAT AGAAGAAACT GCCCGTAAAC GTGGTACTCC AGTATTGCAG TTTGTTGACG AGGTTAGTGA GTCGTTTAGG GAATTAGTCC ACAAGTCTGG TTTTTGTGCT GATGACTTTA TTCGTACCAC GGAAAGTAGG CATAAAGATG CCGTAGTAGC GTTATGGGAT CGCCTATATA GTAGGGGGCA GATATACAAG GGGAGTTATG CTGGTTTCTA TTCAGTTCGT GATGAGACTT TTTATCAAGA GCGTGATTTA ATTGATGGTA AAGCTCCAAC CGGAGCGGAA GTTACGTGGT TAGAAGAGCC AGGGTATTTT TTTAAGTTAT CAGAATGGCA GGAAGCTCTT CTGCAGTTAT ATCAGGATAA TCCTCGTTTT GTTGTGCCAG AAGGGAAGCT GAGTGAAGTC ATAAGCTTTG TGAAGTCGGG ATTGAGAGAT TTGTCAGTTT CACGTTCGAA AGCACATCTT TCTTGGGGCA TAGGTGTCCC AGGTGATCCA GAGCATTTGA TTTATGTATG GGTAGATGCC CTTTCTAATT ATTTGGCATT AGTGGGGTTT CCTAACGAGG ATGCTGAGGA CTATAACAAC TTTTGGTGTG GTGAAAATTC CCATGTAGTG CATGTTGTAG GTAAAGATAT ACTGAAATTT CATGCGGTTT ACTGGCCTGC CTTGTTGATG GCTGCAGGGT TACCATTGCC TAAACAAGTT GTAGCGCATG GTTGGTGGTT GAATGAAGGG CAGAAGATCT CCAAATCGTT GGGGAATGTG ATATGTCCGC TTACGATAAT AAATGAGTAT GGCTTGGATA ACGTACGCTA CTTTTTGCTT AGTGAGACTC CCATAGGGAA TGATGCTAGC TTCAGCGCTA AGAGGCTAAA GGAGCGTGTA AACTGTGATT TGGCCAATAA TATTGGTAAT TTGGTCCAAA GAACTGTCAC CTTGGTACAT AAGTGGTGTG AAGGAAAAGT TCCTTCTGTA GATATGAAGC TCATGTGTGG TGATGAAGCC CTTCCCGATT ATCAGGGGAT TTTGGAACAA TATAGGGTCT ATGTAGAGGA GCGCCGCTTC TTTGAGGCTC TAAAATTTAT AATAGGTGTA TCTTCTACAG CGAATGAGTA TATAGCTGTA AAAGCGCCTT GGAAGCTATT TAAGGAAGAT ACTGCCTTAG CGGAAGCTGT ACTGTTCAAA TTATTAGAAT ACATAAAGTG TATAGGGTTG CTGTTACAGC CATTTCTTCC TGTGACATCT GAGAAGCTAT TAGAGCAAAT TGCTGTACCT GTAGAGAAGA GAAAGTTTGA GCATATTGCA GAACTATGTA CAGGTAGAGT ACTCCCAAAA GCAGTACCTA TATTCTCCAA GTTTGATATG GATGATGTA

Details for AnphA.10201.a.A1.GE27627

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	60kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSSKHFCVT TPIYYVNDVP HIGHFYTTLI SDVLARFKRL DGDNVKFTTG TDEHGQKIEE TARKRGTPVL QFVDEVSESF RELVHKSGFC ADDFIRTTES RHKDAVVALW DRLYSRGQIY KGSYAGFYSV RDETFYQERD LIDGKAPTGA EVTWLEEPGY FFKLSEWQEA LLQLYQDNPR FVVPEGKLSE VISFVKSGLR DLSVSRSKAH LSWGIGVPGD PXHLIYVWVD ALSNYLALVG FPNEDAEDYN NFWCGENSHV VHVVGKDILK FHAVYWPALL MAAGLPLPKQ VVAHGWWLNE GQKISKSLGN VICPLTIINE YGLDNVRYFL LSETPIGNDA SFSAKRLKER VNCDLANNIG NLVQRTVTLV HKWCEGKVPS VDMKLMCGDE ALPDYQGILE QYRVYVEERR FFEALKFIIG VSSTANEYIA VKAPWKLFKE DTALAEAVLF KLLEYIKCIG LLLQPFLPVT SEKLLEQIAV PVEKRKFEHI AELCTGRVLP KAVPIFSKFD MD

Validated NT Sequence

catccatatc aaacttggag aatataggta ctgcttttgg gagtactcta cctgtacata gttctgcaat atgctcaaac tttctcttct ctacaggtac agcaatttgc tctaatagct tctcagatgt cacaggaaga aatggctgta acagcaaccc tatacacttt atgtattcta ataatttgaa cagtacagct tccgctaagg cagtatcttc cttaaatagc ttccaaggcg cttttacagc tatatactca ttcgctgtag aagatacacc tattataaat tttagagcct caaagaagcg gcgctcctct acatagaccc tatattgttc caaaatcccc tgataatcgg gaagggcttc atcaccacac atgagcttca tatctacaga aggaactttt ccttcacacc acttatgtac caaggtgaca gttctttgga ccaaattacc aatattattg gccaaatcac agtttacacg ctcctttagc ctcttagcgc tgaagctagc atcattccct atgggagtct cactaagcaa aaagtagcgt acgttatcca agccatactc atttattatc gtaagcggac atatcacatt ccccaacgat ttggagatct tctgcccttc attcaaccac caaccatgcg ctacaacttg tttaggcaat ggtaaccctg cagccatcaa caaggcaggc cagtaaaccg catgaaattt cagtatatct ttacctacaa catgcactac atgggaattt tcaccacacc aaaagttgtt atagtcctca gcatcctcgt taggaaaccc cactaatgcc aaataattag aaagggcatc tacccataca taaatcaaat gntctggatc acctgggaca cctatgcccc aagaaagatg tgctttcgaa cgtgaaactg acaaatctct caatcccgac ttcacaaagc ttatgacttc actcagcttc ccttctggca caacaaaacg aggattatcc tgatataact gcagaagagc ttcctgccat tctgataact taaaaaaata ccctggctct tctaaccacg taacttccgc tccggttgga gctttaccat caattaaatc acgctcttga taaaaagtct catcacgaac tgaatagaaa ccagcataac tccccttgta tatctgcccc ctactatata ggcgatccca taacgctact acggcatctt tatgcctact ttccgtggta cgaataaagt catcagcaca aaaaccagac ttgtggacta attccctaaa cgactcacta acctcgtcaa caaactgcaa tactggagta ccacgtttac gggcagtttc ttctattttc tggccatgct catctgttcc ggtagtaaac tttacattat caccatccag acgcttaaac cttgcaagaa catctgatat aagcgtggta taaaagtgcc caatgtgggg aacatcgttc acataatata tgggcgtggt aacacagaag tgcttacttg acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSSKHFCVT TPIYYVNDVP HIGHFYTTLI SDVLARFKRL DGDNVKFTTG TDEHGQKIEE TARKRGTPVL QFVDEVSESF RELVHKSGFC ADDFIRTTES RHKDAVVALW DRLYSRGQIY KGSYAGFYSV RDETFYQERD LIDGKAPTGA EVTWLEEPGY FFKLSEWQEA LLQLYQDNPR FVVPEGKLSE VISFVKSGLR DLSVSRSKAH LSWGIGVPGD PEHLIYVWVD ALSNYLALVG FPNEDAEDYN NFWCGENSHV VHVVGKDILK FHAVYWPALL MAAGLPLPKQ VVAHGWWLNE GQKISKSLGN VICPLTIINE YGLDNVRYFL LSETPIGNDA SFSAKRLKER VNCDLANNIG NLVQRTVTLV HKWCEGKVPS VDMKLMCGDE ALPDYQGILE QYRVYVEERR FFEALKFIIG VSSTANEYIA VKAPWKLFKE DTALAEAVLF KLLEYIKCIG LLLQPFLPVT SEKLLEQIAV PVEKRKFEHI AELCTGRVLP KAVPIFSKFD MDDV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcaagtaag cacttctgtg ttaccacgcc catatattat gtgaacgatg ttccccacat tgggcacttt tataccacgc ttatatcaga tgttcttgca aggtttaagc gtctggatgg tgataatgta aagtttacta ccggaacaga tgagcatggc cagaaaatag aagaaactgc ccgtaaacgt ggtactccag tattgcagtt tgttgacgag gttagtgagt cgtttaggga attagtccac aagtctggtt tttgtgctga tgactttatt cgtaccacgg aaagtaggca taaagatgcc gtagtagcgt tatgggatcg cctatatagt agggggcaga tatacaaggg gagttatgct ggtttctatt cagttcgtga tgagactttt tatcaagagc gtgatttaat tgatggtaaa gctccaaccg gagcggaagt tacgtggtta gaagagccag ggtatttttt taagttatca gaatggcagg aagctcttct gcagttatat caggataatc ctcgttttgt tgtgccagaa gggaagctga gtgaagtcat aagctttgtg aagtcgggat tgagagattt gtcagtttca cgttcgaaag cacatctttc ttggggcata ggtgtcccag gtgatccaga gcatttgatt tatgtatggg tagatgccct ttctaattat ttggcattag tggggtttcc taacgaggat gctgaggact ataacaactt ttggtgtggt gaaaattccc atgtagtgca tgttgtaggt aaagatatac tgaaatttca tgcggtttac tggcctgcct tgttgatggc tgcagggtta ccattgccta aacaagttgt agcgcatggt tggtggttga atgaagggca gaagatctcc aaatcgttgg ggaatgtgat atgtccgctt acgataataa atgagtatgg cttggataac gtacgctact ttttgcttag tgagactccc atagggaatg atgctagctt cagcgctaag aggctaaagg agcgtgtaaa ctgtgatttg gccaataata ttggtaattt ggtccaaaga actgtcacct tggtacataa gtggtgtgaa ggaaaagttc cttctgtaga tatgaagctc atgtgtggtg atgaagccct tcccgattat caggggattt tggaacaata tagggtctat gtagaggagc gccgcttctt tgaggctcta aaatttataa taggtgtatc ttctacagcg aatgagtata tagctgtaaa agcgccttgg aagctattta aggaagatac tgccttagcg gaagctgtac tgttcaaatt attagaatac ataaagtgta tagggttgct gttacagcca tttcttcctg tgacatctga gaagctatta gagcaaattg ctgtacctgt agagaagaga aagtttgagc atattgcaga actatgtaca ggtagagtac tcccaaaagc agtacctata ttctccaagt ttgatatgga tgatgtaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for AnphA.10201.a.A1.PW27984

PURIFICATION DATe:	11/9/2009
CONCENTRATION:	29.35mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSSKHFCVT TPIYYVNDVP HIGHFYTTLI SDVLARFKRL DGDNVKFTTG TDEHGQKIEE TARKRGTPVL QFVDEVSESF RELVHKSGFC ADDFIRTTES RHKDAVVALW DRLYSRGQIY KGSYAGFYSV RDETFYQERD LIDGKAPTGA EVTWLEEPGY FFKLSEWQEA LLQLYQDNPR FVVPEGKLSE VISFVKSGLR DLSVSRSKAH LSWGIGVPGD PEHLIYVWVD ALSNYLALVG FPNEDAEDYN NFWCGENSHV VHVVGKDILK FHAVYWPALL MAAGLPLPKQ VVAHGWWLNE GQKISKSLGN VICPLTIINE YGLDNVRYFL LSETPIGNDA SFSAKRLKER VNCDLANNIG NLVQRTVTLV HKWCEGKVPS VDMKLMCGDE ALPDYQGILE QYRVYVEERR FFEALKFIIG VSSTANEYIA VKAPWKLFKE DTALAEAVLF KLLEYIKCIG LLLQPFLPVT SEKLLEQIAV PVEKRKFEHI AELCTGRVLP KAVPIFSKFD MDDV

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAAGTAAG CACTTCTGTG TTACCACGCC CATATATTAT GTGAACGATG TTCCCCACAT TGGGCACTTT TATACCACGC TTATATCAGA TGTTCTTGCA AGGTTTAAGC GTCTGGATGG TGATAATGTA AAGTTTACTA CCGGAACAGA TGAGCATGGC CAGAAAATAG AAGAAACTGC CCGTAAACGT GGTACTCCAG TATTGCAGTT TGTTGACGAG GTTAGTGAGT CGTTTAGGGA ATTAGTCCAC AAGTCTGGTT TTTGTGCTGA TGACTTTATT CGTACCACGG AAAGTAGGCA TAAAGATGCC GTAGTAGCGT TATGGGATCG CCTATATAGT AGGGGGCAGA TATACAAGGG GAGTTATGCT GGTTTCTATT CAGTTCGTGA TGAGACTTTT TATCAAGAGC GTGATTTAAT TGATGGTAAA GCTCCAACCG GAGCGGAAGT TACGTGGTTA GAAGAGCCAG GGTATTTTTT TAAGTTATCA GAATGGCAGG AAGCTCTTCT GCAGTTATAT CAGGATAATC CTCGTTTTGT TGTGCCAGAA GGGAAGCTGA GTGAAGTCAT AAGCTTTGTG AAGTCGGGAT TGAGAGATTT GTCAGTTTCA CGTTCGAAAG CACATCTTTC TTGGGGCATA GGTGTCCCAG GTGATCCAGA GCATTTGATT TATGTATGGG TAGATGCCCT TTCTAATTAT TTGGCATTAG TGGGGTTTCC TAACGAGGAT GCTGAGGACT ATAACAACTT TTGGTGTGGT GAAAATTCCC ATGTAGTGCA TGTTGTAGGT AAAGATATAC TGAAATTTCA TGCGGTTTAC TGGCCTGCCT TGTTGATGGC TGCAGGGTTA CCATTGCCTA AACAAGTTGT AGCGCATGGT TGGTGGTTGA ATGAAGGGCA GAAGATCTCC AAATCGTTGG GGAATGTGAT ATGTCCGCTT ACGATAATAA ATGAGTATGG CTTGGATAAC GTACGCTACT TTTTGCTTAG TGAGACTCCC ATAGGGAATG ATGCTAGCTT CAGCGCTAAG AGGCTAAAGG AGCGTGTAAA CTGTGATTTG GCCAATAATA TTGGTAATTT GGTCCAAAGA ACTGTCACCT TGGTACATAA GTGGTGTGAA GGAAAAGTTC CTTCTGTAGA TATGAAGCTC ATGTGTGGTG ATGAAGCCCT TCCCGATTAT CAGGGGATTT TGGAACAATA TAGGGTCTAT GTAGAGGAGC GCCGCTTCTT TGAGGCTCTA AAATTTATAA TAGGTGTATC TTCTACAGCG AATGAGTATA TAGCTGTAAA AGCGCCTTGG AAGCTATTTA AGGAAGATAC TGCCTTAGCG GAAGCTGTAC TGTTCAAATT ATTAGAATAC ATAAAGTGTA TAGGGTTGCT GTTACAGCCA TTTCTTCCTG TGACATCTGA GAAGCTATTA GAGCAAATTG CTGTACCTGT AGAGAAGAGA AAGTTTGAGC ATATTGCAGA ACTATGTACA GGTAGAGTAC TCCCAAAAGC AGTACCTATA TTCTCCAAGT TTGATATGGA TGATGTAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA