AnphA.10100.a

protease DO family protein

CENTER ID: AnphA.10100.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.10100.a.A2.GE27626 Mature protein( AnphA.10100.a ) 21 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.10100.a.A2.PS00408 Mature protein( AnphA.10100.a ) 21 490

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1233
RefSeq: YP_505690.1
UniProt: Q2GIW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRFLSFFFF CALVWGHCAF ASEVASSALR VSENNGPSTR GFSDLASKLL PSVVNISTEQ TVEGDDLAGD GGGGSIFGFP GIPRGFFEGF FNSIEPLFVE PPKPRKVISL GSGFIIDESG LIVTNYHVIA NSQEIQVKFS DGTTAKAKVL GQDPKTDLAV LKVDVAKELV SVKLGNSDDA LVGEWVLAIG NPFGLGGSVS VGIISGRARD INIGTASEFL QTDAAINRGH SGGPLFNADG EVIGINTAII SPQGGGNVGV AFAIPSNNAA RVISILSKGE KVEHGWLGVI VQHVTEGMVE PLGLDSAHGA LVSNVVKGSP AEKGGLRVGD VILEYNGKRV EDMSQLTNLI AKTAVNEKVR LLVLRGGKQV TLKITIGKLE EGADGASGKN GSLETGTESL GITVGDMPEG DGKKRDGVVV LRVDNRGAAF AEGIRRGDVI VGIDAVLVRN VADFTSELEK ILQSTKKDSV LLLVQRGDSP PIYVAVKLKR
NT Sequence
GTGCGTAGAT TCTTATCGTT TTTTTTCTTT TGTGCTTTAG TTTGGGGACA TTGTGCTTTT GCGTCTGAGG TAGCGTCTTC TGCACTGCGC GTTAGTGAAA ATAACGGTCC TTCAACGAGG GGTTTTTCAG ATCTCGCAAG TAAGTTGCTA CCTTCAGTTG TGAATATATC TACAGAGCAG ACAGTAGAAG GGGATGATCT CGCGGGAGAT GGTGGTGGAG GATCTATATT TGGATTCCCT GGTATTCCTA GAGGTTTTTT TGAGGGGTTT TTCAATAGTA TAGAGCCTTT ATTTGTTGAG CCTCCAAAGC CAAGAAAGGT GATATCCCTG GGTTCTGGCT TTATAATAGA TGAATCGGGA TTAATAGTAA CTAATTATCA CGTCATTGCA AATTCACAAG AGATACAGGT AAAGTTCAGT GATGGTACGA CGGCTAAGGC GAAAGTTTTA GGGCAAGATC CAAAAACTGA CTTGGCTGTG CTGAAAGTAG ATGTAGCGAA AGAGCTGGTT AGTGTAAAGT TGGGTAATTC CGACGATGCT CTGGTTGGAG AATGGGTTTT GGCTATAGGT AATCCGTTTG GTTTGGGTGG ATCTGTTAGT GTAGGGATAA TCTCCGGCAG AGCGCGCGAT ATTAACATAG GGACCGCGAG TGAATTTTTG CAAACTGATG CTGCGATTAA TCGTGGACAC TCTGGAGGCC CACTATTTAA TGCGGATGGG GAGGTTATCG GTATCAATAC CGCGATTATC TCACCGCAGG GGGGTGGTAA CGTGGGAGTT GCGTTTGCAA TACCCTCTAA CAATGCTGCT CGTGTTATAA GCATACTGTC TAAAGGTGAA AAGGTGGAGC ATGGCTGGCT TGGGGTTATC GTACAGCATG TGACTGAAGG CATGGTTGAA CCGCTAGGAC TAGATTCCGC TCATGGAGCT CTTGTATCTA ATGTGGTAAA GGGTAGTCCA GCTGAAAAGG GTGGATTGCG AGTTGGTGAC GTCATTCTTG AGTACAACGG TAAAAGAGTT GAGGATATGT CGCAGCTAAC GAACCTAATC GCGAAAACAG CTGTGAATGA GAAAGTACGG CTTCTTGTGC TAAGGGGCGG AAAGCAGGTC ACTCTAAAAA TAACGATAGG TAAGTTAGAG GAAGGTGCCG ACGGTGCATC AGGTAAGAAT GGAAGCCTAG AAACAGGAAC TGAAAGCTTA GGGATAACCG TAGGTGATAT GCCTGAGGGC GATGGTAAAA AACGTGACGG AGTAGTTGTG CTACGCGTTG ATAATCGAGG TGCTGCTTTT GCTGAAGGCA TCCGTAGGGG TGATGTAATA GTAGGAATAG ATGCAGTATT AGTACGCAAC GTGGCTGACT TCACGAGTGA GCTAGAAAAG ATACTACAGA GTACTAAGAA GGATTCAGTA TTGTTACTAG TACAGCGCGG AGATTCTCCT CCTATATATG TAGCTGTCAA ACTAAAGCGT
Details for AnphA.10100.a.A2.GE27626
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 51kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASEVASSA LRVSENNGPS TRGFSDLASK LLPSVVNIST EQTVEGDDLA GDGGGGSIFG FPGIPRGFFE GFFNSIEPLF VEPPKPRKVI SLGSGFIIDE SGLIVTNYHV IANSQEIQVK FSDGTTAKAK VLGQDPKTDL AVLKVDVAKE LVSVKLGNSD DALVGEWVLA IGNPFGLGGS VSVGIISGRA RDINIGTASE FLQTDAAINR GHSGGPLFNA DGEVIGINTA IISPQGGGNV GVAFAIPSNN AARVISILSK GEKVEHGWLG VIVQHVTEGM VEPLGLDSAH GALVSNVVKG SPAEKGGLRV GDVILEYNGK RVEDMSQLTN LIAKTAVNEK VRLLVLRGGK QVTLKITIGK LEEGADGASG KNGSLETGTE SLGITVGDMP EGDGKKRDGV VVLRVDNRGA AFAEGIRRGD VIVGIDAVLV RNVADFTSEL EKILQSTKKD SVLLLVQRGD SPPIYVAVKL
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gcgtctgagg tagcgtcttc tgcactgcgc gttagtgaaa ataacggtcc ttcaacgagg ggtttttcag atctcgcaag taagttgcta ccttcagttg tgaatatatc tacagagcag acagtagaag gggatgatct cgcgggagat ggtggtggag gatctatatt tggattccct ggtattccta gaggtttttt tgaggggttt ttcaatagta tagagccttt atttgttgag cctccaaagc caagaaaggt gatatccctg ggttctggct ttataataga tgaatcggga ttaatagtaa ctaattatca cgtcattgca aattcacaag agatacaggt aaagttcagt gatggtacga cggctaaggc gaaagtttta gggcaagatc caaaaactga cttggctgtg ctgaaagtag atgtagcgaa agagctggtt agtgtaaagt tgggtaattc cgacgatgct ctggttggag aatgggtttt ggctataggt aatccgtttg gtttgggtgg atctgttagt gtagggataa tctccggcag agcgcgcgat attaacatag ggaccgcgag tgaatttttg caaactgatg ctgcgattaa tcgtggacac tctggaggcc cactatttaa tgcggatggg gaggttatcg gtatcaatac cgcgattatc tcaccgcagg ggggtggtaa cgtgggagtt gcgtttgcaa taccctctaa caatgctgct cgtgttataa gcatactgtc taaaggtgaa aaggtggagc atggctggct tggggttatc gtacagcatg tgactgaagg catggttgaa ccgctaggac tagattccgc tcatggagct cttgtatcta atgtggtaaa gggtagtcca gctgaaaagg gtggattgcg agttggtgac gtcattcttg agtacaacgg taaaagagtt gaggatatgt cgcagctaac gaacctaatc gcgaaaacag ctgtgaatga gaaagtacgg cttcttgtgc taaggggcgg aaagcaggtc actctaaaaa taacgatagg taagttagag gaaggtgccg acggtgcatc aggtaagaat ggaagcctag aaacaggaac tgaaagctta gggataaccg taggtgatat gcctgagggc gatggtaaaa aacgtgacgg agtagttgtg ctacgcgttg ataatcgagg tgctgctttt gctgaaggca tccgtagggg tgatgtaata gtaggaatag atgcagtatt agtacgcaac gtggctgact tcacgagtga gctagaaaag atactacaga gtactaagaa ggattcagta ttgttactag tacagcgcgg agattctcct cctatatatg tagctgtcaa actaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASEVASSA LRVSENNGPS TRGFSDLASK LLPSVVNIST EQTVEGDDLA GDGGGGSIFG FPGIPRGFFE GFFNSIEPLF VEPPKPRKVI SLGSGFIIDE SGLIVTNYHV IANSQEIQVK FSDGTTAKAK VLGQDPKTDL AVLKVDVAKE LVSVKLGNSD DALVGEWVLA IGNPFGLGGS VSVGIISGRA RDINIGTASE FLQTDAAINR GHSGGPLFNA DGEVIGINTA IISPQGGGNV GVAFAIPSNN AARVISILSK GEKVEHGWLG VIVQHVTEGM VEPLGLDSAH GALVSNVVKG SPAEKGGLRV GDVILEYNGK RVEDMSQLTN LIAKTAVNEK VRLLVLRGGK QVTLKITIGK LEEGADGASG KNGSLETGTE SLGITVGDMP EGDGKKRDGV VVLRVDNRGA AFAEGIRRGD VIVGIDAVLV RNVADFTSEL EKILQSTKKD SVLLLVQRGD SPPIYVAVKL KR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtctgag gtagcgtctt ctgcactgcg cgttagtgaa aataacggtc cttcaacgag gggtttttca gatctcgcaa gtaagttgct accttcagtt gtgaatatat ctacagagca gacagtagaa ggggatgatc tcgcgggaga tggtggtgga ggatctatat ttggattccc tggtattcct agaggttttt ttgaggggtt tttcaatagt atagagcctt tatttgttga gcctccaaag ccaagaaagg tgatatccct gggttctggc tttataatag atgaatcggg attaatagta actaattatc acgtcattgc aaattcacaa gagatacagg taaagttcag tgatggtacg acggctaagg cgaaagtttt agggcaagat ccaaaaactg acttggctgt gctgaaagta gatgtagcga aagagctggt tagtgtaaag ttgggtaatt ccgacgatgc tctggttgga gaatgggttt tggctatagg taatccgttt ggtttgggtg gatctgttag tgtagggata atctccggca gagcgcgcga tattaacata gggaccgcga gtgaattttt gcaaactgat gctgcgatta atcgtggaca ctctggaggc ccactattta atgcggatgg ggaggttatc ggtatcaata ccgcgattat ctcaccgcag gggggtggta acgtgggagt tgcgtttgca ataccctcta acaatgctgc tcgtgttata agcatactgt ctaaaggtga aaaggtggag catggctggc ttggggttat cgtacagcat gtgactgaag gcatggttga accgctagga ctagattccg ctcatggagc tcttgtatct aatgtggtaa agggtagtcc agctgaaaag ggtggattgc gagttggtga cgtcattctt gagtacaacg gtaaaagagt tgaggatatg tcgcagctaa cgaacctaat cgcgaaaaca gctgtgaatg agaaagtacg gcttcttgtg ctaaggggcg gaaagcaggt cactctaaaa ataacgatag gtaagttaga ggaaggtgcc gacggtgcat caggtaagaa tggaagccta gaaacaggaa ctgaaagctt agggataacc gtaggtgata tgcctgaggg cgatggtaaa aaacgtgacg gagtagttgt gctacgcgtt gataatcgag gtgctgcttt tgctgaaggc atccgtaggg gtgatgtaat agtaggaata gatgcagtat tagtacgcaa cgtggctgac ttcacgagtg agctagaaaa gatactacag agtactaaga aggattcagt attgttacta gtacagcgcg gagattctcc tcctatatat gtagctgtca aactaaagcg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.10100.a.A2.PS00408
PURIFICATION DATe: 11/10/2009
CONCENTRATION: 9.42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASEVASSA LRVSENNGPS TRGFSDLASK LLPSVVNIST EQTVEGDDLA GDGGGGSIFG FPGIPRGFFE GFFNSIEPLF VEPPKPRKVI SLGSGFIIDE SGLIVTNYHV IANSQEIQVK FSDGTTAKAK VLGQDPKTDL AVLKVDVAKE LVSVKLGNSD DALVGEWVLA IGNPFGLGGS VSVGIISGRA RDINIGTASE FLQTDAAINR GHSGGPLFNA DGEVIGINTA IISPQGGGNV GVAFAIPSNN AARVISILSK GEKVEHGWLG VIVQHVTEGM VEPLGLDSAH GALVSNVVKG SPAEKGGLRV GDVILEYNGK RVEDMSQLTN LIAKTAVNEK VRLLVLRGGK QVTLKITIGK LEEGADGASG KNGSLETGTE SLGITVGDMP EGDGKKRDGV VVLRVDNRGA AFAEGIRRGD VIVGIDAVLV RNVADFTSEL EKILQSTKKD SVLLLVQRGD SPPIYVAVKL
Validated NT Sequence
taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gcgtctgagg tagcgtcttc tgcactgcgc gttagtgaaa ataacggtcc ttcaacgagg ggtttttcag atctcgcaag taagttgcta ccttcagttg tgaatatatc tacagagcag acagtagaag gggatgatct cgcgggagat ggtggtggag gatctatatt tggattccct ggtattccta gaggtttttt tgaggggttt ttcaatagta tagagccttt atttgttgag cctccaaagc caagaaaggt gatatccctg ggttctggct ttataataga tgaatcggga ttaatagtaa ctaattatca cgtcattgca aattcacaag agatacaggt aaagttcagt gatggtacga cggctaaggc gaaagtttta gggcaagatc caaaaactga cttggctgtg ctgaaagtag atgtagcgaa agagctggtt agtgtaaagt tgggtaattc cgacgatgct ctggttggag aatgggtttt ggctataggt aatccgtttg gtttgggtgg atctgttagt gtagggataa tctccggcag agcgcgcgat attaacatag ggaccgcgag tgaatttttg caaactgatg ctgcgattaa tcgtggacac tctggaggcc cactatttaa tgcggatggg gaggttatcg gtatcaatac cgcgattatc tcaccgcagg ggggtggtaa cgtgggagtt gcgtttgcaa taccctctaa caatgctgct cgtgttataa gcatactgtc taaaggtgaa aaggtggagc atggctggct tggggttatc gtacagcatg tgactgaagg catggttgaa ccgctaggac tagattccgc tcatggagct cttgtatcta atgtggtaaa gggtagtcca gctgaaaagg gtggattgcg agttggtgac gtcattcttg agtacaacgg taaaagagtt gaggatatgt cgcagctaac gaacctaatc gcgaaaacag ctgtgaatga gaaagtacgg cttcttgtgc taaggggcgg aaagcaggtc actctaaaaa taacgatagg taagttagag gaaggtgccg acggtgcatc aggtaagaat ggaagcctag aaacaggaac tgaaagctta gggataaccg taggtgatat gcctgagggc gatggtaaaa aacgtgacgg agtagttgtg ctacgcgttg ataatcgagg tgctgctttt gctgaaggca tccgtagggg tgatgtaata gtaggaatag atgcagtatt agtacgcaac gtggctgact tcacgagtga gctagaaaag atactacaga gtactaagaa ggattcagta ttgttactag tacagcgcgg agattctcct cctatatatg tagctgtcaa actaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASEVASSA LRVSENNGPS TRGFSDLASK LLPSVVNIST EQTVEGDDLA GDGGGGSIFG FPGIPRGFFE GFFNSIEPLF VEPPKPRKVI SLGSGFIIDE SGLIVTNYHV IANSQEIQVK FSDGTTAKAK VLGQDPKTDL AVLKVDVAKE LVSVKLGNSD DALVGEWVLA IGNPFGLGGS VSVGIISGRA RDINIGTASE FLQTDAAINR GHSGGPLFNA DGEVIGINTA IISPQGGGNV GVAFAIPSNN AARVISILSK GEKVEHGWLG VIVQHVTEGM VEPLGLDSAH GALVSNVVKG SPAEKGGLRV GDVILEYNGK RVEDMSQLTN LIAKTAVNEK VRLLVLRGGK QVTLKITIGK LEEGADGASG KNGSLETGTE SLGITVGDMP EGDGKKRDGV VVLRVDNRGA AFAEGIRRGD VIVGIDAVLV RNVADFTSEL EKILQSTKKD SVLLLVQRGD SPPIYVAVKL KR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTCTGAG GTAGCGTCTT CTGCACTGCG CGTTAGTGAA AATAACGGTC CTTCAACGAG GGGTTTTTCA GATCTCGCAA GTAAGTTGCT ACCTTCAGTT GTGAATATAT CTACAGAGCA GACAGTAGAA GGGGATGATC TCGCGGGAGA TGGTGGTGGA GGATCTATAT TTGGATTCCC TGGTATTCCT AGAGGTTTTT TTGAGGGGTT TTTCAATAGT ATAGAGCCTT TATTTGTTGA GCCTCCAAAG CCAAGAAAGG TGATATCCCT GGGTTCTGGC TTTATAATAG ATGAATCGGG ATTAATAGTA ACTAATTATC ACGTCATTGC AAATTCACAA GAGATACAGG TAAAGTTCAG TGATGGTACG ACGGCTAAGG CGAAAGTTTT AGGGCAAGAT CCAAAAACTG ACTTGGCTGT GCTGAAAGTA GATGTAGCGA AAGAGCTGGT TAGTGTAAAG TTGGGTAATT CCGACGATGC TCTGGTTGGA GAATGGGTTT TGGCTATAGG TAATCCGTTT GGTTTGGGTG GATCTGTTAG TGTAGGGATA ATCTCCGGCA GAGCGCGCGA TATTAACATA GGGACCGCGA GTGAATTTTT GCAAACTGAT GCTGCGATTA ATCGTGGACA CTCTGGAGGC CCACTATTTA ATGCGGATGG GGAGGTTATC GGTATCAATA CCGCGATTAT CTCACCGCAG GGGGGTGGTA ACGTGGGAGT TGCGTTTGCA ATACCCTCTA ACAATGCTGC TCGTGTTATA AGCATACTGT CTAAAGGTGA AAAGGTGGAG CATGGCTGGC TTGGGGTTAT CGTACAGCAT GTGACTGAAG GCATGGTTGA ACCGCTAGGA CTAGATTCCG CTCATGGAGC TCTTGTATCT AATGTGGTAA AGGGTAGTCC AGCTGAAAAG GGTGGATTGC GAGTTGGTGA CGTCATTCTT GAGTACAACG GTAAAAGAGT TGAGGATATG TCGCAGCTAA CGAACCTAAT CGCGAAAACA GCTGTGAATG AGAAAGTACG GCTTCTTGTG CTAAGGGGCG GAAAGCAGGT CACTCTAAAA ATAACGATAG GTAAGTTAGA GGAAGGTGCC GACGGTGCAT CAGGTAAGAA TGGAAGCCTA GAAACAGGAA CTGAAAGCTT AGGGATAACC GTAGGTGATA TGCCTGAGGG CGATGGTAAA AAACGTGACG GAGTAGTTGT GCTACGCGTT GATAATCGAG GTGCTGCTTT TGCTGAAGGC ATCCGTAGGG GTGATGTAAT AGTAGGAATA GATGCAGTAT TAGTACGCAA CGTGGCTGAC TTCACGAGTG AGCTAGAAAA GATACTACAG AGTACTAAGA AGGATTCAGT ATTGTTACTA GTACAGCGCG GAGATTCTCC TCCTATATAT GTAGCTGTCA AACTAAAGCG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA