AnphA.01597.a

thymidylate kinase

CENTER ID: AnphA.01597.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01597.a.A1.GE27621 Full length( AnphA.01597.a ) 1 219
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01597.a.A1.PW27484 Full length( AnphA.01597.a ) 1 219
AnphA.01597.a.A1.PW29385 Full length( AnphA.01597.a ) 1 219
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126920
PATRIC ID: fig|212042.8.peg.99
RefSeq: YP_504721.1
UniProt: Q2GLN0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFITFEGIDG CGKTTQSILL AKYMSDLYGE DNVVLTREPG GTSFNELLRS VFLSVSDYKV DKLTELFLFL AMRRESFVKV VESALRANKI VISDRCVDST VAYQGYGCGI DLDLIYKLNS LVMGIVPDMT FIIDIDIEKA LSRATRNGYE SNSMDFYHKV RKGFQRIAEE EKHRCVLLRC DDYEENGICD VYSVHNKIVG LLQGVLHNKM DAASSSVKV
NT Sequence
ATGTTCATAA CTTTTGAGGG AATAGATGGG TGTGGTAAGA CCACGCAGTC TATTTTACTA GCGAAGTATA TGTCTGATCT GTACGGAGAA GACAATGTGG TGTTGACTAG AGAACCTGGG GGCACTTCTT TTAATGAACT GCTGCGTAGC GTGTTTTTGT CAGTTTCAGA CTACAAGGTT GATAAGCTCA CAGAGTTATT TCTATTTCTA GCAATGCGCA GAGAGAGCTT TGTTAAGGTG GTAGAGAGTG CTCTGAGAGC GAATAAGATA GTAATTAGTG ACCGCTGTGT AGACTCTACT GTGGCTTACC AAGGGTACGG TTGTGGTATT GACCTGGATC TAATATACAA GCTTAATTCC CTGGTTATGG GAATAGTTCC AGATATGACG TTCATCATAG ACATCGATAT AGAAAAGGCC TTATCAAGGG CTACTCGTAA TGGCTATGAG AGCAATAGCA TGGATTTTTA TCACAAGGTG CGGAAGGGCT TTCAACGTAT AGCTGAGGAA GAGAAACATA GATGTGTATT GCTCCGTTGT GATGATTACG AGGAGAATGG TATCTGTGAT GTTTATTCCG TACACAACAA AATAGTGGGG CTTTTGCAGG GGGTGCTTCA TAACAAGATG GATGCCGCCT CCTCTAGTGT AAAGGTT
Details for AnphA.01597.a.A1.GE27621
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFITFEGID GCGKTTQSIL LAKYMSDLYG EDNVVLTREP GGTSFNELLR SVFLSVSDYK VDKLTELFLF LAMRRESFVK VVESALRANK IVISDRCVDS TVAYQGYGCG IDLDLIYKLN SLVMGIVPDM TFIIDIDIEK ALSRATRNGY ESNSMDFYHK VRKGFQRIAE EEKHRCVLLR CDDYEENGIC DVYSVHNKIV GLLQGVLHNK MDAASSSV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca taacttttga gggaatagat gggtgtggta agaccacgca gtctatttta ctagcgaagt atatgtctga tctgtacgga gaagacaatg tggtgttgac tagagaacct gggggcactt cttttaatga actgctgcgt agcgtgtttt tgtcagtttc agactacaag gttgataagc tcacagagtt atttctattt ctagcaatgc gcagagagag ctttgttaag gtggtagaga gtgctctgag agcgaataag atagtaatta gtgaccgctg tgtagactct actgtggctt accaagggta cggttgtggt attgacctgg atctaatata caagcttaat tccctggtta tgggaatagt tccagatatg acgttcatca tagacatcga tatagaaaag gccttatcaa gggctactcg taatggctat gagagcaata gcatggattt ttatcacaag gtgcggaagg gctttcaacg tatagctgag gaagagaaac atagatgtgt attgctccgt tgtgatgatt acgaggagaa tggtatctgt gatgtttatt ccgtacacaa caaaatagtg gggcttttgc agggggtgct tcataacaag atggatgccg cctcctctag tgtaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFITFEGID GCGKTTQSIL LAKYMSDLYG EDNVVLTREP GGTSFNELLR SVFLSVSDYK VDKLTELFLF LAMRRESFVK VVESALRANK IVISDRCVDS TVAYQGYGCG IDLDLIYKLN SLVMGIVPDM TFIIDIDIEK ALSRATRNGY ESNSMDFYHK VRKGFQRIAE EEKHRCVLLR CDDYEENGIC DVYSVHNKIV GLLQGVLHNK MDAASSSVKV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcataact tttgagggaa tagatgggtg tggtaagacc acgcagtcta ttttactagc gaagtatatg tctgatctgt acggagaaga caatgtggtg ttgactagag aacctggggg cacttctttt aatgaactgc tgcgtagcgt gtttttgtca gtttcagact acaaggttga taagctcaca gagttatttc tatttctagc aatgcgcaga gagagctttg ttaaggtggt agagagtgct ctgagagcga ataagatagt aattagtgac cgctgtgtag actctactgt ggcttaccaa gggtacggtt gtggtattga cctggatcta atatacaagc ttaattccct ggttatggga atagttccag atatgacgtt catcatagac atcgatatag aaaaggcctt atcaagggct actcgtaatg gctatgagag caatagcatg gatttttatc acaaggtgcg gaagggcttt caacgtatag ctgaggaaga gaaacataga tgtgtattgc tccgttgtga tgattacgag gagaatggta tctgtgatgt ttattccgta cacaacaaaa tagtggggct tttgcagggg gtgcttcata acaagatgga tgccgcctcc tctagtgtaa aggttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for AnphA.01597.a.A1.PW27484
PURIFICATION DATe: 4/5/2010
CONCENTRATION: 27.42mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFITFEGID GCGKTTQSIL LAKYMSDLYG EDNVVLTREP GGTSFNELLR SVFLSVSDYK VDKLTELFLF LAMRRESFVK VVESALRANK IVISDRCVDS TVAYQGYGCG IDLDLIYKLN SLVMGIVPDM TFIIDIDIEK ALSRATRNGY ESNSMDFYHK VRKGFQRIAE EEKHRCVLLR CDDYEENGIC DVYSVHNKIV GLLQGVLHNK MDAASSSV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca taacttttga gggaatagat gggtgtggta agaccacgca gtctatttta ctagcgaagt atatgtctga tctgtacgga gaagacaatg tggtgttgac tagagaacct gggggcactt cttttaatga actgctgcgt agcgtgtttt tgtcagtttc agactacaag gttgataagc tcacagagtt atttctattt ctagcaatgc gcagagagag ctttgttaag gtggtagaga gtgctctgag agcgaataag atagtaatta gtgaccgctg tgtagactct actgtggctt accaagggta cggttgtggt attgacctgg atctaatata caagcttaat tccctggtta tgggaatagt tccagatatg acgttcatca tagacatcga tatagaaaag gccttatcaa gggctactcg taatggctat gagagcaata gcatggattt ttatcacaag gtgcggaagg gctttcaacg tatagctgag gaagagaaac atagatgtgt attgctccgt tgtgatgatt acgaggagaa tggtatctgt gatgtttatt ccgtacacaa caaaatagtg gggcttttgc agggggtgct tcataacaag atggatgccg cctcctctag tgtaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFITFEGID GCGKTTQSIL LAKYMSDLYG EDNVVLTREP GGTSFNELLR SVFLSVSDYK VDKLTELFLF LAMRRESFVK VVESALRANK IVISDRCVDS TVAYQGYGCG IDLDLIYKLN SLVMGIVPDM TFIIDIDIEK ALSRATRNGY ESNSMDFYHK VRKGFQRIAE EEKHRCVLLR CDDYEENGIC DVYSVHNKIV GLLQGVLHNK MDAASSSVKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCATAACT TTTGAGGGAA TAGATGGGTG TGGTAAGACC ACGCAGTCTA TTTTACTAGC GAAGTATATG TCTGATCTGT ACGGAGAAGA CAATGTGGTG TTGACTAGAG AACCTGGGGG CACTTCTTTT AATGAACTGC TGCGTAGCGT GTTTTTGTCA GTTTCAGACT ACAAGGTTGA TAAGCTCACA GAGTTATTTC TATTTCTAGC AATGCGCAGA GAGAGCTTTG TTAAGGTGGT AGAGAGTGCT CTGAGAGCGA ATAAGATAGT AATTAGTGAC CGCTGTGTAG ACTCTACTGT GGCTTACCAA GGGTACGGTT GTGGTATTGA CCTGGATCTA ATATACAAGC TTAATTCCCT GGTTATGGGA ATAGTTCCAG ATATGACGTT CATCATAGAC ATCGATATAG AAAAGGCCTT ATCAAGGGCT ACTCGTAATG GCTATGAGAG CAATAGCATG GATTTTTATC ACAAGGTGCG GAAGGGCTTT CAACGTATAG CTGAGGAAGA GAAACATAGA TGTGTATTGC TCCGTTGTGA TGATTACGAG GAGAATGGTA TCTGTGATGT TTATTCCGTA CACAACAAAA TAGTGGGGCT TTTGCAGGGG GTGCTTCATA ACAAGATGGA TGCCGCCTCC TCTAGTGTAA AGGTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for AnphA.01597.a.A1.PW29385
PURIFICATION DATe: 4/5/2010
CONCENTRATION: 27.33mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFITFEGID GCGKTTQSIL LAKYMSDLYG EDNVVLTREP GGTSFNELLR SVFLSVSDYK VDKLTELFLF LAMRRESFVK VVESALRANK IVISDRCVDS TVAYQGYGCG IDLDLIYKLN SLVMGIVPDM TFIIDIDIEK ALSRATRNGY ESNSMDFYHK VRKGFQRIAE EEKHRCVLLR CDDYEENGIC DVYSVHNKIV GLLQGVLHNK MDAASSSV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca taacttttga gggaatagat gggtgtggta agaccacgca gtctatttta ctagcgaagt atatgtctga tctgtacgga gaagacaatg tggtgttgac tagagaacct gggggcactt cttttaatga actgctgcgt agcgtgtttt tgtcagtttc agactacaag gttgataagc tcacagagtt atttctattt ctagcaatgc gcagagagag ctttgttaag gtggtagaga gtgctctgag agcgaataag atagtaatta gtgaccgctg tgtagactct actgtggctt accaagggta cggttgtggt attgacctgg atctaatata caagcttaat tccctggtta tgggaatagt tccagatatg acgttcatca tagacatcga tatagaaaag gccttatcaa gggctactcg taatggctat gagagcaata gcatggattt ttatcacaag gtgcggaagg gctttcaacg tatagctgag gaagagaaac atagatgtgt attgctccgt tgtgatgatt acgaggagaa tggtatctgt gatgtttatt ccgtacacaa caaaatagtg gggcttttgc agggggtgct tcataacaag atggatgccg cctcctctag tgtaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFITFEGID GCGKTTQSIL LAKYMSDLYG EDNVVLTREP GGTSFNELLR SVFLSVSDYK VDKLTELFLF LAMRRESFVK VVESALRANK IVISDRCVDS TVAYQGYGCG IDLDLIYKLN SLVMGIVPDM TFIIDIDIEK ALSRATRNGY ESNSMDFYHK VRKGFQRIAE EEKHRCVLLR CDDYEENGIC DVYSVHNKIV GLLQGVLHNK MDAASSSVKV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCATAACT TTTGAGGGAA TAGATGGGTG TGGTAAGACC ACGCAGTCTA TTTTACTAGC GAAGTATATG TCTGATCTGT ACGGAGAAGA CAATGTGGTG TTGACTAGAG AACCTGGGGG CACTTCTTTT AATGAACTGC TGCGTAGCGT GTTTTTGTCA GTTTCAGACT ACAAGGTTGA TAAGCTCACA GAGTTATTTC TATTTCTAGC AATGCGCAGA GAGAGCTTTG TTAAGGTGGT AGAGAGTGCT CTGAGAGCGA ATAAGATAGT AATTAGTGAC CGCTGTGTAG ACTCTACTGT GGCTTACCAA GGGTACGGTT GTGGTATTGA CCTGGATCTA ATATACAAGC TTAATTCCCT GGTTATGGGA ATAGTTCCAG ATATGACGTT CATCATAGAC ATCGATATAG AAAAGGCCTT ATCAAGGGCT ACTCGTAATG GCTATGAGAG CAATAGCATG GATTTTTATC ACAAGGTGCG GAAGGGCTTT CAACGTATAG CTGAGGAAGA GAAACATAGA TGTGTATTGC TCCGTTGTGA TGATTACGAG GAGAATGGTA TCTGTGATGT TTATTCCGTA CACAACAAAA TAGTGGGGCT TTTGCAGGGG GTGCTTCATA ACAAGATGGA TGCCGCCTCC TCTAGTGTAA AGGTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA