SSGCID: Target Page

AnphA.01473.a

pyruvate dehydrogenase subunit beta

CENTER ID:	AnphA.01473.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01473.a.A1.GE27069	Full length( AnphA.01473.a )		1	332

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1401
RefSeq:	YP_505822.1
UniProt:	Q2GIH9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEVITVREAL RLAMEEEMER DQSVFLMGEE VGEYQGAYKI SQGLLERFGP QRVVDTPISE HGFTGLAVGA AFCGLKPIVE FMSFNFSMQA MDQIVNSAAK TNYMSGGQLG CPIVFRGPNG AAAGVAAQHS QCFASWYSHV PGIKVVAPYF AADCKGLLKS AIRDPNPVIF LENEIAYGHS HEVTEEQLSK DSLVELGKAA IVREGKDVTI ITFSLQLKYA LEAAEILLKD NISAEVIDLR TLRPLDTEAI LKSVKKTNRV VTVEEGWPFC GVGAEITALI DECAFDDLDA PVTRVTAKDV PLPYAANLES LALPGVEDIV SAVHKVCNYS IA

NT Sequence

ATGGAAGTTA TAACTGTAAG GGAAGCATTA CGCTTGGCTA TGGAAGAGGA GATGGAGCGT GACCAGAGTG TTTTTCTTAT GGGGGAAGAA GTTGGAGAAT ACCAAGGTGC GTACAAAATA AGTCAGGGGC TTTTAGAGCG CTTTGGGCCA CAAAGGGTTG TCGATACTCC TATTAGTGAA CACGGTTTTA CTGGATTGGC CGTAGGGGCG GCTTTTTGCG GATTAAAGCC TATAGTAGAG TTTATGAGCT TTAATTTCTC TATGCAGGCT ATGGACCAGA TAGTTAATTC CGCTGCGAAA ACAAATTACA TGTCAGGGGG GCAGCTAGGT TGTCCTATAG TCTTCCGTGG GCCTAATGGT GCTGCAGCAG GTGTTGCAGC TCAACACTCC CAGTGTTTTG CATCGTGGTA TTCACACGTG CCTGGTATAA AGGTTGTTGC TCCATATTTT GCTGCGGATT GTAAGGGGTT ATTGAAATCT GCAATACGCG ATCCAAATCC TGTAATATTT CTAGAAAATG AGATTGCGTA CGGACACTCG CACGAGGTGA CAGAAGAGCA GTTGAGCAAA GATAGTTTGG TTGAGCTTGG TAAAGCGGCA ATTGTGAGAG AAGGTAAAGA TGTAACGATT ATAACATTCT CTTTACAGTT GAAGTATGCT CTTGAGGCTG CTGAGATTCT GTTGAAGGAC AATATCAGTG CGGAGGTTAT AGATCTTAGG ACATTGCGTC CTCTGGATAC TGAGGCTATT TTAAAGTCTG TCAAAAAGAC GAATCGTGTC GTTACTGTTG AAGAGGGATG GCCGTTTTGT GGTGTGGGAG CAGAGATTAC AGCGTTGATA GATGAATGTG CCTTTGATGA TCTTGATGCA CCTGTCACGA GGGTAACTGC CAAAGATGTT CCGTTACCTT ATGCAGCCAA TTTGGAAAGT CTCGCCCTCC CTGGCGTGGA AGATATAGTC TCAGCAGTGC ATAAGGTGTG TAATTATTCT ATAGCT

Details for AnphA.01473.a.A1.GE27069

HARVESTED ON:	7/6/2009
SEQUENCED ON:	7/21/2009
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMEVITVR EALRLAMEEE MERDQSVFLM GEEVGEYQGA YKISQGLLER FGPQRVVDTP ISEHGFTGLA VGAAFCGLKP IVEFMSFNFS MQAMDQIVNS AAKTNYMSGG QLGCPIVFRG PNGAAAGVAA QHSQCFASWY SHVPGIKVVA PYFAADCKGL LKSAIRDPNP VIFLENEIAY GHSHEVTEEQ LSKDSLVELG KAAIVREGKD VTIITFSLQL KYALEAAEIL LKDNISAEVI DLRTLRPLDT EAILKSVKKT NRVVTVEEGW PFCGVGAEIT ALIDECAFDD LDAPVTRVTA KDVPLPYAAN LESLALPGVE DIVSAVHKVC NYSIA

Validated NT Sequence

catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggaagt tataactgta agggaagcat tacgcttggc tatggaagag gagatggagc gtgaccagag tgtttttctt atgggggaag aagttggaga ataccaaggt gcgtacaaaa taagtcaggg gcttttagag cgctttgggc cacaaagggt tgtcgatact cctattagtg aacacggttt tactggattg gccgtagggg cggctttttg cggattaaag cctatagtag agtttatgag ctttaatttc tctatgcagg ctatggacca gatagttaat tccgctgcga aaacaaatta catgtcaggg gggcagctag gttgtcctat agtcttccgt gggcctaatg gtgctgcagc aggtgttgca gctcaacact cccagtgttt tgcatcgtgg tattcacacg tgcctggtat aaaggttgtt gctccatatt ttgctgcgga ttgtaagggg ttattgaaat ctgcaatacg cgatccaaat cctgtaatat ttctagaaaa tgagattgcg tacggacact cgcacgaggt gacagaagag cagttgagca aagatagttt ggttgagctt ggtaaagcgg caattgtgag agaaggtaaa gatgtaacga ttataacatt ctctttacag ttgaagtatg ctcttgaggc tgctgagatt ctgttgaagg acaatatcag tgcggaggtt atagatctta ggacattgcg tcctctggat actgaggcta ttttaaagtc tgtcaaaaag acgaatcgtg tcgttactgt tgaagaggga tggccgtttt gtggtgtggg agcagagatt acagcgttga tagatgaatg tgcctttgat gatcttgatg cacctgtcac gagggtaact gccaaagatg ttccgttacc ttatgcagcc aatttggaaa gtctcgccct ccctggcgtg gaagatatag tctcagcagt gcataaggtg tgtaattatt ctatagctta ataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEVITVREA LRLAMEEEME RDQSVFLMGE EVGEYQGAYK ISQGLLERFG PQRVVDTPIS EHGFTGLAVG AAFCGLKPIV EFMSFNFSMQ AMDQIVNSAA KTNYMSGGQL GCPIVFRGPN GAAAGVAAQH SQCFASWYSH VPGIKVVAPY FAADCKGLLK SAIRDPNPVI FLENEIAYGH SHEVTEEQLS KDSLVELGKA AIVREGKDVT IITFSLQLKY ALEAAEILLK DNISAEVIDL RTLRPLDTEA ILKSVKKTNR VVTVEEGWPF CGVGAEITAL IDECAFDDLD APVTRVTAKD VPLPYAANLE SLALPGVEDI VSAVHKVCNY SIA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaagttata actgtaaggg aagcattacg cttggctatg gaagaggaga tggagcgtga ccagagtgtt tttcttatgg gggaagaagt tggagaatac caaggtgcgt acaaaataag tcaggggctt ttagagcgct ttgggccaca aagggttgtc gatactccta ttagtgaaca cggttttact ggattggccg taggggcggc tttttgcgga ttaaagccta tagtagagtt tatgagcttt aatttctcta tgcaggctat ggaccagata gttaattccg ctgcgaaaac aaattacatg tcaggggggc agctaggttg tcctatagtc ttccgtgggc ctaatggtgc tgcagcaggt gttgcagctc aacactccca gtgttttgca tcgtggtatt cacacgtgcc tggtataaag gttgttgctc catattttgc tgcggattgt aaggggttat tgaaatctgc aatacgcgat ccaaatcctg taatatttct agaaaatgag attgcgtacg gacactcgca cgaggtgaca gaagagcagt tgagcaaaga tagtttggtt gagcttggta aagcggcaat tgtgagagaa ggtaaagatg taacgattat aacattctct ttacagttga agtatgctct tgaggctgct gagattctgt tgaaggacaa tatcagtgcg gaggttatag atcttaggac attgcgtcct ctggatactg aggctatttt aaagtctgtc aaaaagacga atcgtgtcgt tactgttgaa gagggatggc cgttttgtgg tgtgggagca gagattacag cgttgataga tgaatgtgcc tttgatgatc ttgatgcacc tgtcacgagg gtaactgcca aagatgttcc gttaccttat gcagccaatt tggaaagtct cgccctccct ggcgtggaag atatagtctc agcagtgcat aaggtgtgta attattctat agctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa