AnphA.01463.a
guanylate kinase
| CENTER ID: | AnphA.01463.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01463.a.A1.GE27068 | Full length( AnphA.01463.a ) | 1 | 210 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01463.a.A1.PS00342 | Full length( AnphA.01463.a ) | 1 | 210 |
Structures
3LNC
DEPOSITED: 2/2/2010
DETERMINATION: XRay
CLONE: AnphA.01463.a.A1.GE27068
PROTEIN: AnphA.01463.a.A1.PS00342
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126916 |
| PATRIC ID: | fig|212042.8.peg.179 |
| RefSeq: | YP_504794.1 |
| UniProt: | Q2GLF7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKSVGVILV LSSPSGCGKT TVANKLLEKQ KNNIVKSVSV TTRAARKGEK EGKDYYFVDR EEFLRLCSNG EIIEHAEVFG NFYGVPRKNL EDNVDKGVST LLVIDWQGAF KFMEMMREHV VSIFIMPPSM EELRRRLCGR RADDSEVVEA RLKGAAFEIS HCEAYDYVIV NEDIEETADR ISNILRAEQM KTCRQVGLRE LLESRFPIED
NT Sequence
TTGTTGAAAA GCGTAGGTGT AATACTGGTA TTATCGTCAC CGTCTGGGTG CGGAAAAACA ACAGTTGCCA ACAAATTATT AGAGAAGCAG AAAAACAATA TAGTAAAATC CGTTTCCGTC ACGACTCGCG CAGCAAGGAA AGGAGAGAAA GAAGGGAAAG ACTACTATTT TGTAGATCGG GAAGAATTTT TAAGACTATG CAGCAACGGG GAGATAATAG AACATGCAGA AGTATTTGGT AATTTCTACG GTGTTCCCCG CAAGAATCTA GAAGACAATG TAGATAAGGG AGTCAGCACG TTACTAGTAA TTGACTGGCA AGGAGCTTTC AAGTTCATGG AGATGATGAG AGAGCACGTA GTGAGCATAT TCATAATGCC CCCTTCTATG GAAGAACTGC GTAGGAGATT ATGTGGTAGA AGAGCTGATG ATTCAGAGGT TGTGGAAGCA AGGCTAAAAG GAGCAGCGTT TGAGATCAGT CACTGCGAAG CTTACGACTA CGTAATAGTA AACGAAGATA TAGAGGAAAC AGCAGACAGA ATTAGCAATA TCCTGAGGGC AGAACAGATG AAAACTTGCA GGCAAGTTGG GCTGAGGGAA TTATTAGAGA GTAGGTTCCC TATAGAAGAT
Details for AnphA.01463.a.A1.GE27068
| HARVESTED ON: | 7/6/2009 |
| SEQUENCED ON: | 7/21/2009 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLLKSVGVI LVLSSPSGCG KTTVANKLLE KQKNNIVKSV SVTTRAARKG EKEGKDYYFV DREEFLRLCS NGEIIEHAEV FGNFYGVPRK NLEDNVDKGV STLLVIDWQG AFKFMEMMRE HVVSIFIMPP SMEELRRRLC GRRADDSEVV EARLKGAAFE ISHCEAYDYV IVNEDIEETA DRISNILRAE QMKTCRQVGL RELLESRFPI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt tgaaaagcgt aggtgtaata ctggtattat cgtcaccgtc tgggtgcgga aaaacaacag ttgccaacaa attattagag aagcagaaaa acaatatagt aaaatccgtt tccgtcacga ctcgcgcagc aaggaaagga gagaaagaag ggaaagacta ctattttgta gatcgggaag aatttttaag actatgcagc aacggggaga taatagaaca tgcagaagta tttggtaatt tctacggtgt tccccgcaag aatctagaag acaatgtaga taagggagtc agcacgttac tagtaattga ctggcaagga gctttcaagt tcatggagat gatgagagag cacgtagtga gcatattcat aatgccccct tctatggaag aactgcgtag gagattatgt ggtagaagag ctgatgattc agaggttgtg gaagcaaggc taaaaggagc agcgtttgag atcagtcact gcgaagctta cgactacgta atagtaaacg aagatataga ggaaacagca gacagaatta gcaatatcct gagggcagaa cagatgaaaa cttgcaggca agttgggctg agggaattat tagagagtag gttccctata g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLKSVGVI LVLSSPSGCG KTTVANKLLE KQKNNIVKSV SVTTRAARKG EKEGKDYYFV DREEFLRLCS NGEIIEHAEV FGNFYGVPRK NLEDNVDKGV STLLVIDWQG AFKFMEMMRE HVVSIFIMPP SMEELRRRLC GRRADDSEVV EARLKGAAFE ISHCEAYDYV IVNEDIEETA DRISNILRAE QMKTCRQVGL RELLESRFPI ED
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgttgaaa agcgtaggtg taatactggt attatcgtca ccgtctgggt gcggaaaaac aacagttgcc aacaaattat tagagaagca gaaaaacaat atagtaaaat ccgtttccgt cacgactcgc gcagcaagga aaggagagaa agaagggaaa gactactatt ttgtagatcg ggaagaattt ttaagactat gcagcaacgg ggagataata gaacatgcag aagtatttgg taatttctac ggtgttcccc gcaagaatct agaagacaat gtagataagg gagtcagcac gttactagta attgactggc aaggagcttt caagttcatg gagatgatga gagagcacgt agtgagcata ttcataatgc ccccttctat ggaagaactg cgtaggagat tatgtggtag aagagctgat gattcagagg ttgtggaagc aaggctaaaa ggagcagcgt ttgagatcag tcactgcgaa gcttacgact acgtaatagt aaacgaagat atagaggaaa cagcagacag aattagcaat atcctgaggg cagaacagat gaaaacttgc aggcaagttg ggctgaggga attattagag agtaggttcc ctatagaaga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.01463.a.A1.PS00342
| PURIFICATION DATe: | 9/2/2009 |
| CONCENTRATION: | 52.01mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLLKSVGVI LVLSSPSGCG KTTVANKLLE KQKNNIVKSV SVTTRAARKG EKEGKDYYFV DREEFLRLCS NGEIIEHAEV FGNFYGVPRK NLEDNVDKGV STLLVIDWQG AFKFMEMMRE HVVSIFIMPP SMEELRRRLC GRRADDSEVV EARLKGAAFE ISHCEAYDYV IVNEDIEETA DRISNILRAE QMKTCRQVGL RELLESRFPI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgt tgaaaagcgt aggtgtaata ctggtattat cgtcaccgtc tgggtgcgga aaaacaacag ttgccaacaa attattagag aagcagaaaa acaatatagt aaaatccgtt tccgtcacga ctcgcgcagc aaggaaagga gagaaagaag ggaaagacta ctattttgta gatcgggaag aatttttaag actatgcagc aacggggaga taatagaaca tgcagaagta tttggtaatt tctacggtgt tccccgcaag aatctagaag acaatgtaga taagggagtc agcacgttac tagtaattga ctggcaagga gctttcaagt tcatggagat gatgagagag cacgtagtga gcatattcat aatgccccct tctatggaag aactgcgtag gagattatgt ggtagaagag ctgatgattc agaggttgtg gaagcaaggc taaaaggagc agcgtttgag atcagtcact gcgaagctta cgactacgta atagtaaacg aagatataga ggaaacagca gacagaatta gcaatatcct gagggcagaa cagatgaaaa cttgcaggca agttgggctg agggaattat tagagagtag gttccctata g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLLKSVGVI LVLSSPSGCG KTTVANKLLE KQKNNIVKSV SVTTRAARKG EKEGKDYYFV DREEFLRLCS NGEIIEHAEV FGNFYGVPRK NLEDNVDKGV STLLVIDWQG AFKFMEMMRE HVVSIFIMPP SMEELRRRLC GRRADDSEVV EARLKGAAFE ISHCEAYDYV IVNEDIEETA DRISNILRAE QMKTCRQVGL RELLESRFPI ED
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTTGAAA AGCGTAGGTG TAATACTGGT ATTATCGTCA CCGTCTGGGT GCGGAAAAAC AACAGTTGCC AACAAATTAT TAGAGAAGCA GAAAAACAAT ATAGTAAAAT CCGTTTCCGT CACGACTCGC GCAGCAAGGA AAGGAGAGAA AGAAGGGAAA GACTACTATT TTGTAGATCG GGAAGAATTT TTAAGACTAT GCAGCAACGG GGAGATAATA GAACATGCAG AAGTATTTGG TAATTTCTAC GGTGTTCCCC GCAAGAATCT AGAAGACAAT GTAGATAAGG GAGTCAGCAC GTTACTAGTA ATTGACTGGC AAGGAGCTTT CAAGTTCATG GAGATGATGA GAGAGCACGT AGTGAGCATA TTCATAATGC CCCCTTCTAT GGAAGAACTG CGTAGGAGAT TATGTGGTAG AAGAGCTGAT GATTCAGAGG TTGTGGAAGC AAGGCTAAAA GGAGCAGCGT TTGAGATCAG TCACTGCGAA GCTTACGACT ACGTAATAGT AAACGAAGAT ATAGAGGAAA CAGCAGACAG AATTAGCAAT ATCCTGAGGG CAGAACAGAT GAAAACTTGC AGGCAAGTTG GGCTGAGGGA ATTATTAGAG AGTAGGTTCC CTATAGAAGA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA