AnphA.01360.a
Fe superoxide dismutase
| CENTER ID: | AnphA.01360.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01360.a.A1.GE27048 | Full length( AnphA.01360.a ) | 1 | 206 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01360.a.A1.PW27056 | Full length( AnphA.01360.a ) | 1 | 206 |
Structures
3JS4
DEPOSITED: 9/9/2009
DETERMINATION: XRay
CLONE: AnphA.01360.a.A1.GE27048
PROTEIN: AnphA.01360.a.A1.PW27056
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126676 |
| PATRIC ID: | fig|212042.8.peg.394 |
| RefSeq: | YP_504977.1 |
| UniProt: | Q2GKX4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFELSDLPYE GLEPYISSHL LDRHYNGHHK TYVDVLNKLV VGTEFEGLGN ESLGDIVVKA HNSGSAGRAI FNNAAQIWNH DFYWQSMKPN GGGNPPEKLR EMIEHSFGSV EGFNNAFTTS GLGQFGSGWV WLVYDEDAKA LKVVSTANAD SPLLTQGQLP LATMDVWEHA YYLDYLNLRK KYIDVFLEHL LNWDFVLGRL EDAGVL
NT Sequence
ATGTTTGAAT TGTCTGATCT GCCGTATGAG GGGTTGGAGC CGTATATTTC TTCCCATCTC TTAGATCGTC ACTATAATGG TCACCACAAA ACTTATGTTG ATGTTCTAAA CAAGTTGGTG GTGGGTACTG AGTTTGAAGG TTTGGGTAAC GAAAGCTTAG GGGATATAGT AGTCAAGGCT CACAATAGTG GGAGTGCTGG TAGGGCTATA TTCAACAATG CTGCGCAAAT ATGGAATCAC GATTTCTATT GGCAATCCAT GAAGCCGAAT GGTGGGGGAA ATCCTCCAGA GAAGCTTAGA GAGATGATAG AGCACAGTTT CGGTAGTGTG GAAGGGTTTA ATAATGCCTT TACTACTAGT GGTCTTGGGC AGTTCGGTAG TGGATGGGTA TGGCTTGTAT ATGATGAAGA TGCTAAGGCA TTGAAAGTTG TTAGCACAGC GAATGCAGAT TCACCGCTTC TTACTCAGGG GCAGCTTCCT TTGGCAACTA TGGATGTTTG GGAGCATGCT TACTATTTAG ATTATCTCAA CCTAAGGAAG AAATATATAG ACGTTTTCCT AGAGCATTTG CTTAACTGGG ATTTTGTCTT GGGTAGACTG GAAGATGCAG GTGTACTG
Details for AnphA.01360.a.A1.GE27048
| HARVESTED ON: | 7/6/2009 |
| SEQUENCED ON: | 7/21/2009 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFELSDLPY EGLEPYISSH LLDRHYNGHH KTYVDVLNKL VVGTEFEGLG NESLGDIVVK AHNSGSAGRA IFNNAAQIWN HDFYWQSMKP NGGGNPPEKL REMIEHSFGS VEGFNNAFTT SGLGQFGSGW VWLVYDEDAK ALKVVSTANA DSPLLTQGQL PLATMDVWEH AYYLDYLNLR KKYIDVFLEH LLNWDFVLGR LEDAGVL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtttg aattgtctga tctgccgtat gaggggttgg agccgtatat ttcttcccat ctcttagatc gtcactataa tggtcaccac aaaacttatg ttgatgttct aaacaagttg gtggtgggta ctgagtttga aggtttgggt aacgaaagct taggggatat agtagtcaag gctcacaata gtgggagtgc tggtagggct atattcaaca atgctgcgca aatatggaat cacgatttct attggcaatc catgaagccg aatggtgggg gaaatcctcc agagaagctt agagagatga tagagcacag tttcggtagt gtggaagggt ttaataatgc ctttactact agtggtcttg ggcagttcgg tagtggatgg gtatggcttg tatatgatga agatgctaag gcattgaaag ttgttagcac agcgaatgca gattcaccgc ttcttactca ggggcagctt cctttggcaa ctatggatgt ttgggagcat gcttactatt tagattatct caacctaagg aagaaatata tagacgtttt cctagagcat ttgcttaact gggattttgt cttgggtaga ctggaagatg caggtgtact gtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFELSDLPY EGLEPYISSH LLDRHYNGHH KTYVDVLNKL VVGTEFEGLG NESLGDIVVK AHNSGSAGRA IFNNAAQIWN HDFYWQSMKP NGGGNPPEKL REMIEHSFGS VEGFNNAFTT SGLGQFGSGW VWLVYDEDAK ALKVVSTANA DSPLLTQGQL PLATMDVWEH AYYLDYLNLR KKYIDVFLEH LLNWDFVLGR LEDAGVL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttgaattg tctgatctgc cgtatgaggg gttggagccg tatatttctt cccatctctt agatcgtcac tataatggtc accacaaaac ttatgttgat gttctaaaca agttggtggt gggtactgag tttgaaggtt tgggtaacga aagcttaggg gatatagtag tcaaggctca caatagtggg agtgctggta gggctatatt caacaatgct gcgcaaatat ggaatcacga tttctattgg caatccatga agccgaatgg tgggggaaat cctccagaga agcttagaga gatgatagag cacagtttcg gtagtgtgga agggtttaat aatgccttta ctactagtgg tcttgggcag ttcggtagtg gatgggtatg gcttgtatat gatgaagatg ctaaggcatt gaaagttgtt agcacagcga atgcagattc accgcttctt actcaggggc agcttccttt ggcaactatg gatgtttggg agcatgctta ctatttagat tatctcaacc taaggaagaa atatatagac gttttcctag agcatttgct taactgggat tttgtcttgg gtagactgga agatgcaggt gtactgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for AnphA.01360.a.A1.PW27056
| PURIFICATION DATe: | 7/14/2009 |
| CONCENTRATION: | 30.01mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 23 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFELSDLPY EGLEPYISSH LLDRHYNGHH KTYVDVLNKL VVGTEFEGLG NESLGDIVVK AHNSGSAGRA IFNNAAQIWN HDFYWQSMKP NGGGNPPEKL REMIEHSFGS VEGFNNAFTT SGLGQFGSGW VWLVYDEDAK ALKVVSTANA DSPLLTQGQL PLATMDVWEH AYYLDYLNLR KKYIDVFLEH LLNWDFVLGR LEDAGVL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtttg aattgtctga tctgccgtat gaggggttgg agccgtatat ttcttcccat ctcttagatc gtcactataa tggtcaccac aaaacttatg ttgatgttct aaacaagttg gtggtgggta ctgagtttga aggtttgggt aacgaaagct taggggatat agtagtcaag gctcacaata gtgggagtgc tggtagggct atattcaaca atgctgcgca aatatggaat cacgatttct attggcaatc catgaagccg aatggtgggg gaaatcctcc agagaagctt agagagatga tagagcacag tttcggtagt gtggaagggt ttaataatgc ctttactact agtggtcttg ggcagttcgg tagtggatgg gtatggcttg tatatgatga agatgctaag gcattgaaag ttgttagcac agcgaatgca gattcaccgc ttcttactca ggggcagctt cctttggcaa ctatggatgt ttgggagcat gcttactatt tagattatct caacctaagg aagaaatata tagacgtttt cctagagcat ttgcttaact gggattttgt cttgggtaga ctggaagatg caggtgtact gtaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFELSDLPY EGLEPYISSH LLDRHYNGHH KTYVDVLNKL VVGTEFEGLG NESLGDIVVK AHNSGSAGRA IFNNAAQIWN HDFYWQSMKP NGGGNPPEKL REMIEHSFGS VEGFNNAFTT SGLGQFGSGW VWLVYDEDAK ALKVVSTANA DSPLLTQGQL PLATMDVWEH AYYLDYLNLR KKYIDVFLEH LLNWDFVLGR LEDAGVL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTTGAATTG TCTGATCTGC CGTATGAGGG GTTGGAGCCG TATATTTCTT CCCATCTCTT AGATCGTCAC TATAATGGTC ACCACAAAAC TTATGTTGAT GTTCTAAACA AGTTGGTGGT GGGTACTGAG TTTGAAGGTT TGGGTAACGA AAGCTTAGGG GATATAGTAG TCAAGGCTCA CAATAGTGGG AGTGCTGGTA GGGCTATATT CAACAATGCT GCGCAAATAT GGAATCACGA TTTCTATTGG CAATCCATGA AGCCGAATGG TGGGGGAAAT CCTCCAGAGA AGCTTAGAGA GATGATAGAG CACAGTTTCG GTAGTGTGGA AGGGTTTAAT AATGCCTTTA CTACTAGTGG TCTTGGGCAG TTCGGTAGTG GATGGGTATG GCTTGTATAT GATGAAGATG CTAAGGCATT GAAAGTTGTT AGCACAGCGA ATGCAGATTC ACCGCTTCTT ACTCAGGGGC AGCTTCCTTT GGCAACTATG GATGTTTGGG AGCATGCTTA CTATTTAGAT TATCTCAACC TAAGGAAGAA ATATATAGAC GTTTTCCTAG AGCATTTGCT TAACTGGGAT TTTGTCTTGG GTAGACTGGA AGATGCAGGT GTACTGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA