AnphA.01256.a

Type IV secretion system protein VirB11

CENTER ID: AnphA.01256.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01256.a.A1.GE30741 Full length( AnphA.01256.a ) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1498
RefSeq: YP_505895.1
UniProt: Q2GIA6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTGGGAALET YLEPLRDIFA EEGVNEISIN NSCEVWVENR GNIRCEHIAA LTTSHLRGLG RLIAQATEQK LSEETPLLSA SLPNGFRVQV VFPPACEGDK IVMSIRKPSA MQLSLDDYEK MGAFSHVAQQ NDKLRDENSE LGELLSKGKI KEFLETAVQK KKNIIVSGGT STGKTTFTNA ALRAIPLEER IITVEDSREI VLSHPNRVHL LASKGGQGRA RVGTQDLIEA CLRLRPDRII VGELRGAEAF SFLRAINTGH PGSISTLHAD TPRMAIEQLK LMVIQAGTGL PADQIVNYIM NIVDVIVQLK RSSGGVRHVS DILFTKCSEG NK
NT Sequence
ATGACTGGGG GTGGTGCAGC TTTAGAAACT TATCTTGAAC CGCTTCGGGA TATATTTGCT GAAGAAGGAG TAAATGAAAT ATCAATAAAT AACTCATGTG AAGTATGGGT TGAAAATAGG GGAAATATTA GGTGCGAGCA TATTGCAGCA TTGACGACTT CTCATCTCAG GGGGTTAGGG CGTCTTATTG CTCAGGCTAC GGAGCAAAAG TTAAGTGAGG AAACGCCACT GCTTTCTGCG TCTTTACCCA ACGGGTTTCG TGTACAGGTA GTTTTTCCTC CGGCATGTGA GGGTGATAAG ATAGTGATGT CGATTCGTAA GCCTTCTGCG ATGCAGTTGT CGCTTGATGA TTATGAAAAA ATGGGGGCAT TTTCTCATGT TGCTCAGCAG AATGACAAGC TCAGAGATGA AAATAGCGAA TTAGGTGAGT TGCTATCTAA GGGTAAAATA AAGGAATTTT TAGAAACGGC GGTACAAAAA AAGAAGAATA TAATTGTGAG TGGTGGTACC TCTACAGGGA AGACTACATT TACTAACGCT GCCTTGAGAG CTATACCTTT AGAAGAGAGG ATTATTACTG TTGAAGACTC GAGGGAAATT GTCCTATCTC ATCCAAATCG CGTACATTTA CTTGCTTCTA AAGGTGGGCA AGGTAGAGCA CGTGTTGGTA CGCAAGATTT GATCGAAGCA TGTCTTCGTC TTCGTCCGGA TAGGATTATA GTGGGGGAGT TGAGGGGTGC TGAAGCCTTT AGTTTTTTGA GGGCTATTAA TACTGGGCAT CCTGGATCTA TTTCTACGTT GCACGCTGAT ACTCCAAGGA TGGCTATAGA GCAGTTAAAG CTTATGGTAA TACAGGCAGG AACGGGATTA CCAGCAGATC AGATTGTGAA TTACATAATG AATATTGTGG ATGTGATAGT GCAGCTGAAG AGGAGCTCTG GTGGAGTGCG CCATGTTTCA GACATTTTGT TCACTAAGTG TTCAGAGGGT AATAAG
Details for AnphA.01256.a.A1.GE30741
HARVESTED ON: 11/9/2010
SEQUENCED ON: 11/15/2010
EXPECTED MW: 38kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Validated AA Sequence
MTGGGAXXET YLEPLRDIFA EEGVNEISIN NSCEVWVENR GNIRCEHIAA LTTSHLRGLG RLIAQATEQK LSEETPLLSA SLPNGFRVQV VFPPACEGDK IVMSIRKPSA MQLSLDDYEK MGAFSHVAQQ NDKLRDENSE LGELLSKGKI KEFLETAVQK KKNIIVSGGT STGKTTFTNA ALRAIPLEER IITVEDSREI VLSHPNRVHL LASKGGQGRA RVGTQDLIEA CLRLRPDRII VGELRGAEAF SFLRAINTGH PGSISTLHAD TPRMAIEQLK LMVIQAGTGL PADQIVNYIM NIVDVIVQLK RSSGGVRHVS DILFTKCSEG
Validated NT Sequence
taccctctga acacttagtg aacaaaatgt ctgaaacatg gcgcactcca ccagagctcc tcttcagctg cactatcaca tccacaatat tcattatgta attcacaatc tgatctgctg gtaatcccgt tcctgcctgt attaccataa gctttaactg ctctatagcc atccttggag tatcagcgtg caacgtagaa atagatccag gatgcccagt attaatagcc ctcaaaaaac taaaggcttc agcacccctc aactccccca ctataatcct atccggacga agacgaagac atgcttcgat caaatcttgc gtaccaacac gtgctctacc ttgcccacct ttagaagcaa gtaaatgtac gcgatttgga tgagatagga caatttccct cgagtcttca acagtaataa tcctctcttc taaaggtata gctctcaagg cagcgttagt aaatgtagtc ttccctgtag aggtaccacc actcacaatt atattcttct ttttttgtac cgccgtttct aaaaattcct ttattttacc cttagatagc aactcaccta attcgctatt ttcatctctg agcttgtcat tctgctgagc aacatgagaa aatgccccca ttttttcata atcatcaagc gacaactgca tcgcagaagg cttacgaatc gacatcacta tcttatcacc ctcacatgcc ggaggaaaaa ctacctgtac acgaaacccg ttgggtaaag acgcagaaag cagtggcgtt tcctcactta acttttgctc cgtagcctga gcaataagac gccctaaccc cctgagatga gaagtcgtca atgctgcaat atgctcgcac ctaatatttc ccctattttc aacccatact tcacatgagt tatttattga tatttcattt actccttctt cagcaaatat atcccgaagc ggttcaagat aagtttcnan agntgcacca cccccagtca tcganccagg accctgggtc tgagcttcca ggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGGGAALE TYLEPLRDIF AEEGVNEISI NNSCEVWVEN RGNIRCEHIA ALTTSHLRGL GRLIAQATEQ KLSEETPLLS ASLPNGFRVQ VVFPPACEGD KIVMSIRKPS AMQLSLDDYE KMGAFSHVAQ QNDKLRDENS ELGELLSKGK IKEFLETAVQ KKKNIIVSGG TSTGKTTFTN AALRAIPLEE RIITVEDSRE IVLSHPNRVH LLASKGGQGR ARVGTQDLIE ACLRLRPDRI IVGELRGAEA FSFLRAINTG HPGSISTLHA DTPRMAIEQL KLMVIQAGTG LPADQIVNYI MNIVDVIVQL KRSSGGVRHV SDILFTKCSE GNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgggggt ggtgcagctt tagaaactta tcttgaaccg cttcgggata tatttgctga agaaggagta aatgaaatat caataaataa ctcatgtgaa gtatgggttg aaaatagggg aaatattagg tgcgagcata ttgcagcatt gacgacttct catctcaggg ggttagggcg tcttattgct caggctacgg agcaaaagtt aagtgaggaa acgccactgc tttctgcgtc tttacccaac gggtttcgtg tacaggtagt ttttcctccg gcatgtgagg gtgataagat agtgatgtcg attcgtaagc cttctgcgat gcagttgtcg cttgatgatt atgaaaaaat gggggcattt tctcatgttg ctcagcagaa tgacaagctc agagatgaaa atagcgaatt aggtgagttg ctatctaagg gtaaaataaa ggaattttta gaaacggcgg tacaaaaaaa gaagaatata attgtgagtg gtggtacctc tacagggaag actacattta ctaacgctgc cttgagagct atacctttag aagagaggat tattactgtt gaagactcga gggaaattgt cctatctcat ccaaatcgcg tacatttact tgcttctaaa ggtgggcaag gtagagcacg tgttggtacg caagatttga tcgaagcatg tcttcgtctt cgtccggata ggattatagt gggggagttg aggggtgctg aagcctttag ttttttgagg gctattaata ctgggcatcc tggatctatt tctacgttgc acgctgatac tccaaggatg gctatagagc agttaaagct tatggtaata caggcaggaa cgggattacc agcagatcag attgtgaatt acataatgaa tattgtggat gtgatagtgc agctgaagag gagctctggt ggagtgcgcc atgtttcaga cattttgttc actaagtgtt cagagggtaa taagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa