AnphA.01233.a
O-methyltransferase family protein
| CENTER ID: | AnphA.01233.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01233.a.B1.GE35906 | full length | 1 | 218 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01233.a.B1.PS01686 | full length | 1 | 218 |
Structures
4OA5
DEPOSITED: 1/3/2014
DETERMINATION: XRay
CLONE: AnphA.01233.a.B1.GE35906
PROTEIN: AnphA.01233.a.B1.PS01685
4OA8
DEPOSITED: 1/3/2014
DETERMINATION: XRay
CLONE: AnphA.01233.a.B1.GE35906
PROTEIN: AnphA.01233.a.B1.PS01686
4PCA
DEPOSITED: 4/14/2014
DETERMINATION: XRay
CLONE: AnphA.01233.a.B1.GE35906
PROTEIN: AnphA.01233.a.B1.PS01686
4PCL
DEPOSITED: 4/15/2014
DETERMINATION: XRay
CLONE: AnphA.01233.a.B1.GE35906
PROTEIN: AnphA.01233.a.B1.PS01686
Publications by SSGCID
An O-Methyltransferase Is Required for Infection of Tick Cells by Anaplasma phagocytophilum.
Burkhardt NY, Edwards TE, Fairman JW, Felsheim RF, Herron MJ, Higgins L, Kurtti TJ, Markowski TW, Munderloh UG, Nelson CM, Oliver JD, Olivia Chavez AS
PLoS Pathog. - 2015
volume 11, issue 11, pages e1005248
PMID: 26544981; PMCID: PMC4636158
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128394 |
| PATRIC ID: | fig|212042.8.peg.630 |
| RefSeq: | YP_505174.1 |
| UniProt: | Q2GKC7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRNVSLSKQD EYLNKLFAVD TEGALKAHKT APSELRMAQL GTVEGQMLQL LIRMAGIHSI VEVGTCVGFS AICMAHALPS KGHIYTIEKD YENVVTANQN IVNCKLEDKI TVLHGEALAQ LNTLKEMAPF DMIFIDANKS SYLAYLNWAK MYIRKGGLIV ADNTFLFGSV FDEHPTEKVS SNAHASMRAF NDELANKEKY LSTIIPTSEG MMVSIKLT
NT Sequence
gtgcgcaatg tctctttaag caaacaagat gagtacctta ataagctttt tgctgtagat actgagggtg cattgaaggc gcataaaact gcaccttctg agttgcgtat ggcacaatta gggactgttg aaggacaaat gctacagctt ttaataagaa tggctggtat acattcaata gtcgaagtgg gtacttgtgt gggattttcc gcaatatgta tggcgcatgc cctgccttct aagggacata tatacacaat agagaaagat tacgaaaacg tagtgaccgc aaatcagaac atagtcaact gtaaactgga agataaaata acggtattgc atggtgaggc tttggcgcag ctaaacactt taaaggaaat ggctccattt gatatgatat ttatagatgc aaacaaatcc tcataccttg cttacttaaa ctgggctaaa atgtatataa ggaagggtgg cctcatagtt gctgataaca ccttcctctt tggcagcgta tttgatgagc acccaactga aaaagtttct agtaatgctc atgccagtat gcgagcgttt aatgacgaac tcgctaataa agaaaaatac ctctctacca ttatacctac tagcgaaggc atgatggtat cgataaagct cacatga
Details for AnphA.01233.a.B1.GE35906
| HARVESTED ON: | 8/28/2012 |
| SEQUENCED ON: | 8/31/2012 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV RNVSLSKQDE YLNKLFAVDT EGALKAHKTA PSELRMAQLG TVEGQMLQLL IRMAGIHSIV EVGTCVGFSA ICMAHALPSK GHIYTIEKDY ENVVTANQNI VNCKLEDKIT VLHGEALAQL NTLKEMAPFD MIFIDANKSS YLAYLNWAKM YIRKGGLIVA DNTFLFGSVF DEHPTEKVSS NAHASMRAFN DELANKEKYL STIIPTSEGM MVSIKLT
Validated NT Sequence
agccggatcc tatcttactc acttatgtga gctttatcga taccatcatg ccttcgctag taggtataat ggtagagagg tatttttctt tattagcgag ttcgtcatta aacgctcgca tactggcatg agcattacta gaaacttttt cagttgggtg ctcatcaaat acgctgccaa agaggaaggt gttatcagca actatgaggc cacccttcct tatatacatt ttagcccagt ttaagtaagc aaggtatgag gatttgtttg catctataaa tatcatatca aatggagcca tttcctttaa agtgtttagc tgcgccaaag cctcaccatg caataccgtt attttatctt ccagtttaca gttgactatg ttctgatttg cggtcactac gttttcgtaa tctttctcta ttgtgtatat atgtccctta gaaggcaggg catgcgccat acatattgcg gaaaatccca cacaagtacc cacttcgact attgaatgta taccagccat tcttattaaa agctgtagca tttgtccttc aacagtccct aattgtgcca tacgcaactc agaaggtgca gttttatgcg ccttcaatgc accctcagta tctacagcaa aaagcttatt aaggtactca tcttgtttgc ttaaagagac attgcgcacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMV RNVSLSKQDE YLNKLFAVDT EGALKAHKTA PSELRMAQLG TVEGQMLQLL IRMAGIHSIV EVGTCVGFSA ICMAHALPSK GHIYTIEKDY ENVVTANQNI VNCKLEDKIT VLHGEALAQL NTLKEMAPFD MIFIDANKSS YLAYLNWAKM YIRKGGLIVA DNTFLFGSVF DEHPTEKVSS NAHASMRAFN DELANKEKYL STIIPTSEGM MVSIKLT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc gcaatgtctc tttaagcaaa caagatgagt accttaataa gctttttgct gtagatactg agggtgcatt gaaggcgcat aaaactgcac cttctgagtt gcgtatggca caattaggga ctgttgaagg acaaatgcta cagcttttaa taagaatggc tggtatacat tcaatagtcg aagtgggtac ttgtgtggga ttttccgcaa tatgtatggc gcatgccctg ccttctaagg gacatatata cacaatagag aaagattacg aaaacgtagt gaccgcaaat cagaacatag tcaactgtaa actggaagat aaaataacgg tattgcatgg tgaggctttg gcgcagctaa acactttaaa ggaaatggct ccatttgata tgatatttat agatgcaaac aaatcctcat accttgctta cttaaactgg gctaaaatgt atataaggaa gggtggcctc atagttgctg ataacacctt cctctttggc agcgtatttg atgagcaccc aactgaaaaa gtttctagta atgctcatgc cagtatgcga gcgtttaatg acgaactcgc taataaagaa aaatacctct ctaccattat acctactagc gaaggcatga tggtatcgat aaagctcaca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for AnphA.01233.a.B1.PS01686
| PURIFICATION DATe: | 11/20/2012 |
| CONCENTRATION: | 27.6mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV RNVSLSKQDE YLNKLFAVDT EGALKAHKTA PSELRMAQLG TVEGQMLQLL IRMAGIHSIV EVGTCVGFSA ICMAHALPSK GHIYTIEKDY ENVVTANQNI VNCKLEDKIT VLHGEALAQL NTLKEMAPFD MIFIDANKSS YLAYLNWAKM YIRKGGLIVA DNTFLFGSVF DEHPTEKVSS NAHASMRAFN DELANKEKYL STIIPTSEGM MVSIKLT
Validated NT Sequence
agccggatcc tatcttactc acttatgtga gctttatcga taccatcatg ccttcgctag taggtataat ggtagagagg tatttttctt tattagcgag ttcgtcatta aacgctcgca tactggcatg agcattacta gaaacttttt cagttgggtg ctcatcaaat acgctgccaa agaggaaggt gttatcagca actatgaggc cacccttcct tatatacatt ttagcccagt ttaagtaagc aaggtatgag gatttgtttg catctataaa tatcatatca aatggagcca tttcctttaa agtgtttagc tgcgccaaag cctcaccatg caataccgtt attttatctt ccagtttaca gttgactatg ttctgatttg cggtcactac gttttcgtaa tctttctcta ttgtgtatat atgtccctta gaaggcaggg catgcgccat acatattgcg gaaaatccca cacaagtacc cacttcgact attgaatgta taccagccat tcttattaaa agctgtagca tttgtccttc aacagtccct aattgtgcca tacgcaactc agaaggtgca gttttatgcg ccttcaatgc accctcagta tctacagcaa aaagcttatt aaggtactca tcttgtttgc ttaaagagac attgcgcacc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMV RNVSLSKQDE YLNKLFAVDT EGALKAHKTA PSELRMAQLG TVEGQMLQLL IRMAGIHSIV EVGTCVGFSA ICMAHALPSK GHIYTIEKDY ENVVTANQNI VNCKLEDKIT VLHGEALAQL NTLKEMAPFD MIFIDANKSS YLAYLNWAKM YIRKGGLIVA DNTFLFGSVF DEHPTEKVSS NAHASMRAFN DELANKEKYL STIIPTSEGM MVSIKLT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTGC GCAATGTCTC TTTAAGCAAA CAAGATGAGT ACCTTAATAA GCTTTTTGCT GTAGATACTG AGGGTGCATT GAAGGCGCAT AAAACTGCAC CTTCTGAGTT GCGTATGGCA CAATTAGGGA CTGTTGAAGG ACAAATGCTA CAGCTTTTAA TAAGAATGGC TGGTATACAT TCAATAGTCG AAGTGGGTAC TTGTGTGGGA TTTTCCGCAA TATGTATGGC GCATGCCCTG CCTTCTAAGG GACATATATA CACAATAGAG AAAGATTACG AAAACGTAGT GACCGCAAAT CAGAACATAG TCAACTGTAA ACTGGAAGAT AAAATAACGG TATTGCATGG TGAGGCTTTG GCGCAGCTAA ACACTTTAAA GGAAATGGCT CCATTTGATA TGATATTTAT AGATGCAAAC AAATCCTCAT ACCTTGCTTA CTTAAACTGG GCTAAAATGT ATATAAGGAA GGGTGGCCTC ATAGTTGCTG ATAACACCTT CCTCTTTGGC AGCGTATTTG ATGAGCACCC AACTGAAAAA GTTTCTAGTA ATGCTCATGC CAGTATGCGA GCGTTTAATG ACGAACTCGC TAATAAAGAA AAATACCTCT CTACCATTAT ACCTACTAGC GAAGGCATGA TGGTATCGAT AAAGCTCACA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT