SSGCID: Target Page

AnphA.01172.a

serine hydroxymethyltransferase

CENTER ID:	AnphA.01172.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01172.a.A1.GE27031	Full length( AnphA.01172.a )		1	425

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01172.a.A1.PW27002	Full length( AnphA.01172.a )		1	425

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.163
RefSeq:	YP_504778.1
UniProt:	Q2GLH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVGYIGDIHI SESDAEVAEC LSAEYKRQNT SLQMIASENF VSRAVLQAQG SVLTNKYAEG YPGSRYYCGC SEVDVAETLA VERLCKLFGC KYANVQPHSG SQANQQVYMA LLKPGDTVLG MSLDSGGHLT HGAGPNVSGK WFNAVPYNVR RDTNLLDMGE IEEIALRVKP NLIIAGASSY PRRIDFKAFR AIADKVGAYF LADIAHYSGL IAGGQYPTPF GYAHVVTSTT HKTLRGPRGG VIMTDDEEIH KKLRSAVFPG MQGGALMHVI AAKAVAFREA MSPDFKVYVS QILDNSRALA AVLATGGLDV VTGGTDSHMV VVDLRSKGLT GRDVSSSLER AGIVCNKNAV PFDTEKPWVT SGIRLGAAAE TSRGLVVKDF EKIGQLVLKI VDSMRAGADM SVVESGVREE VATLVRVVPY DTLAC

NT Sequence

ATGGTTGGGT ACATTGGGGA CATCCATATA AGTGAGAGTG ATGCTGAGGT TGCTGAGTGT TTATCTGCTG AGTATAAGCG GCAGAATACG TCTCTGCAGA TGATAGCTTC GGAGAATTTT GTTAGCCGTG CTGTTCTGCA GGCTCAAGGA TCGGTCCTAA CCAACAAATA TGCGGAGGGT TATCCCGGTA GTAGGTATTA CTGTGGTTGT TCTGAGGTAG ACGTTGCGGA GACTTTGGCT GTGGAGCGTC TATGTAAGTT ATTTGGCTGT AAGTACGCTA ATGTTCAGCC GCACTCAGGG TCGCAGGCGA ATCAGCAAGT GTACATGGCG TTATTAAAGC CCGGAGACAC CGTGCTAGGG ATGTCATTGG ACTCGGGAGG GCACTTAACG CATGGAGCGG GGCCTAATGT ATCGGGGAAG TGGTTTAATG CTGTGCCCTA TAATGTTAGG CGTGACACAA ACCTTCTGGA TATGGGTGAG ATAGAAGAGA TTGCTTTGAG GGTGAAGCCT AATTTGATCA TAGCAGGTGC TTCCTCGTAT CCTAGACGAA TAGACTTCAA GGCCTTCAGG GCTATAGCTG ATAAGGTGGG GGCATATTTC CTAGCGGATA TAGCGCATTA CTCAGGGTTA ATAGCAGGAG GTCAGTATCC GACGCCATTT GGCTATGCGC ATGTAGTGAC GTCGACTACG CACAAGACGC TACGTGGTCC TCGCGGAGGC GTGATAATGA CAGATGATGA GGAAATACAC AAAAAGTTAA GGTCGGCGGT ATTTCCTGGC ATGCAGGGAG GGGCTCTGAT GCATGTAATA GCGGCGAAAG CGGTTGCTTT TCGTGAGGCA ATGAGTCCGG ATTTTAAGGT ATATGTTAGC CAGATTCTTG ATAATTCTCG TGCTTTAGCA GCAGTTTTAG CTACTGGAGG GTTGGATGTA GTAACTGGTG GGACTGATAG CCATATGGTG GTTGTTGATT TGCGCTCCAA GGGGCTCACT GGCAGGGATG TTTCGTCTTC GTTGGAGAGA GCTGGGATTG TATGTAATAA AAATGCTGTT CCATTTGATA CTGAAAAGCC GTGGGTAACC TCTGGTATTA GGTTAGGTGC TGCTGCTGAA ACTTCTAGAG GGTTGGTGGT CAAGGATTTT GAGAAGATAG GGCAGCTTGT ATTGAAGATT GTGGACTCTA TGAGGGCTGG AGCGGATATG TCCGTAGTGG AGTCGGGGGT TAGAGAAGAG GTTGCTACTC TTGTTAGGGT AGTGCCGTAC GATACTTTGG CTTGC

Details for AnphA.01172.a.A1.GE27031

HARVESTED ON:	7/6/2009
SEQUENCED ON:	7/21/2009
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MGTLEAQTQG PGSMVGYIGD IHISESDAEV AECLSAEYKR QNTSLQMIAS ENFVSRAVLQ AQGSVLTNKY AEGYPGSRYY CGCSEVDVAE TLAVERLCKL FGCKYANVQP HSGSQANXQV YMALLKPGDT VLGMSLDSGG HLTHGAGPNV SGKWFNAVPY NVRRDTNLLD MGEIEEIALR VKPNLIIAXA SSYPRRIDFK XFRAIADKVG AYFLADIAHY SGLIAGGQYP TPFGYAHVVT XTTHKTLRGP RGGVIMTDDE EIHKKLRSAV FPGMQGGALM HVIAAKAVAF REAMSPDFKV YVSQILDNSR ALAAVLATGG LDVVTGGTDS HMVVVDLRSK GLTGRDVSSS LERAGIVCNK NAVPFDTEKP WVTSGIRLGA AAETSRGLVV KDFEKIGQLV LKIVDSMRAG ADMSVVESGV REEVATLVRV VPYDTLAC

Validated NT Sequence

caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgggtac attggggaca tccatataag tgagagtgat gctgaggttg ctgagtgttt atctgctgag tataagcggc agaatacgtc tctgcagatg atagcttcgg agaattttgt tagccgtgct gttctgcagg ctcaaggatc ggtcctaacc aacaaatatg cggagggtta tcccggtagt aggtattact gtggttgttc tgaggtagac gttgcggaga ctttggctgt ggagcgtcta tgtaagttat ttggctgtaa gtacgctaat gttcagccgc actcagggtc gcaggcgaat cancaagtgt acatggcgtt attaaagccc ggagacaccg tgctagggat gtcattggac tcgggagggc acttaacgca tggagcgggg cctaatgtat cggggaagtg gtttaatgct gtgccctata atgttaggcg tgacacaaac cttctggata tgggtgagat agaagagatt gctttgaggg tgaagcctaa tttgatcata gcangtgctt cctcgtatcc tagacgaata gacttcaagg ncttcagggc tatagctgat aaggtggggg catatttcct agcggatata gcgcattact cagggttaat agcnggaggt cagtatccga cgccatttgg ctatgcgcat gtagtgacgt ngactacgca caagacgcta cgtggtcctc gcggaggcgt gataatgaca gatgatgagg aaatacacaa aaagttaagg tcggcggtat ttcctggcat gcagggaggg gctctgatgc atgtaatagc ggcgaaagcg gttgcttttc gtgaggcaat gagtccggat tttaaggtat atgttagcca gattcttgat aattctcgtg ctttagcagc agttttagct actggagggt tggatgtagt aactggtggg actgatagcc atatggtggt tgttgatttg cgctccaagg ggctcactgg cagggatgtt tcgtcttcgt tggagagagc tgggattgta tgtaataaaa atgctgttcc atttgatact gaaaagccgt gggtaacctc tggtattagg ttaggtgctg ctgctgaaac ttctagaggg ttggtggtca aggattttga gaagataggg cagcttgtat tgaagattgt ggactctatg agggctggag cggatatgtc cgtagtggag tcgggggtta gagaagaggt tgctactctt gttagggtag tgccgtacga tactttggct tgctaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVGYIGDIH ISESDAEVAE CLSAEYKRQN TSLQMIASEN FVSRAVLQAQ GSVLTNKYAE GYPGSRYYCG CSEVDVAETL AVERLCKLFG CKYANVQPHS GSQANQQVYM ALLKPGDTVL GMSLDSGGHL THGAGPNVSG KWFNAVPYNV RRDTNLLDMG EIEEIALRVK PNLIIAGASS YPRRIDFKAF RAIADKVGAY FLADIAHYSG LIAGGQYPTP FGYAHVVTST THKTLRGPRG GVIMTDDEEI HKKLRSAVFP GMQGGALMHV IAAKAVAFRE AMSPDFKVYV SQILDNSRAL AAVLATGGLD VVTGGTDSHM VVVDLRSKGL TGRDVSSSLE RAGIVCNKNA VPFDTEKPWV TSGIRLGAAA ETSRGLVVKD FEKIGQLVLK IVDSMRAGAD MSVVESGVRE EVATLVRVVP YDTLAC

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgggtac attggggaca tccatataag tgagagtgat gctgaggttg ctgagtgttt atctgctgag tataagcggc agaatacgtc tctgcagatg atagcttcgg agaattttgt tagccgtgct gttctgcagg ctcaaggatc ggtcctaacc aacaaatatg cggagggtta tcccggtagt aggtattact gtggttgttc tgaggtagac gttgcggaga ctttggctgt ggagcgtcta tgtaagttat ttggctgtaa gtacgctaat gttcagccgc actcagggtc gcaggcgaat cagcaagtgt acatggcgtt attaaagccc ggagacaccg tgctagggat gtcattggac tcgggagggc acttaacgca tggagcgggg cctaatgtat cggggaagtg gtttaatgct gtgccctata atgttaggcg tgacacaaac cttctggata tgggtgagat agaagagatt gctttgaggg tgaagcctaa tttgatcata gcaggtgctt cctcgtatcc tagacgaata gacttcaagg ccttcagggc tatagctgat aaggtggggg catatttcct agcggatata gcgcattact cagggttaat agcaggaggt cagtatccga cgccatttgg ctatgcgcat gtagtgacgt cgactacgca caagacgcta cgtggtcctc gcggaggcgt gataatgaca gatgatgagg aaatacacaa aaagttaagg tcggcggtat ttcctggcat gcagggaggg gctctgatgc atgtaatagc ggcgaaagcg gttgcttttc gtgaggcaat gagtccggat tttaaggtat atgttagcca gattcttgat aattctcgtg ctttagcagc agttttagct actggagggt tggatgtagt aactggtggg actgatagcc atatggtggt tgttgatttg cgctccaagg ggctcactgg cagggatgtt tcgtcttcgt tggagagagc tgggattgta tgtaataaaa atgctgttcc atttgatact gaaaagccgt gggtaacctc tggtattagg ttaggtgctg ctgctgaaac ttctagaggg ttggtggtca aggattttga gaagataggg cagcttgtat tgaagattgt ggactctatg agggctggag cggatatgtc cgtagtggag tcgggggtta gagaagaggt tgctactctt gttagggtag tgccgtacga tactttggct tgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for AnphA.01172.a.A1.PW27002

PURIFICATION DATe:	7/21/2009
CONCENTRATION:	24.34mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGGGTAC ATTGGGGACA TCCATATAAG TGAGAGTGAT GCTGAGGTTG CTGAGTGTTT ATCTGCTGAG TATAAGCGGC AGAATACGTC TCTGCAGATG ATAGCTTCGG AGAATTTTGT TAGCCGTGCT GTTCTGCAGG CTCAAGGATC GGTCCTAACC AACAAATATG CGGAGGGTTA TCCCGGTAGT AGGTATTACT GTGGTTGTTC TGAGGTAGAC GTTGCGGAGA CTTTGGCTGT GGAGCGTCTA TGTAAGTTAT TTGGCTGTAA GTACGCTAAT GTTCAGCCGC ACTCAGGGTC GCAGGCGAAT CAGCAAGTGT ACATGGCGTT ATTAAAGCCC GGAGACACCG TGCTAGGGAT GTCATTGGAC TCGGGAGGGC ACTTAACGCA TGGAGCGGGG CCTAATGTAT CGGGGAAGTG GTTTAATGCT GTGCCCTATA ATGTTAGGCG TGACACAAAC CTTCTGGATA TGGGTGAGAT AGAAGAGATT GCTTTGAGGG TGAAGCCTAA TTTGATCATA GCAGGTGCTT CCTCGTATCC TAGACGAATA GACTTCAAGG CCTTCAGGGC TATAGCTGAT AAGGTGGGGG CATATTTCCT AGCGGATATA GCGCATTACT CAGGGTTAAT AGCAGGAGGT CAGTATCCGA CGCCATTTGG CTATGCGCAT GTAGTGACGT CGACTACGCA CAAGACGCTA CGTGGTCCTC GCGGAGGCGT GATAATGACA GATGATGAGG AAATACACAA AAAGTTAAGG TCGGCGGTAT TTCCTGGCAT GCAGGGAGGG GCTCTGATGC ATGTAATAGC GGCGAAAGCG GTTGCTTTTC GTGAGGCAAT GAGTCCGGAT TTTAAGGTAT ATGTTAGCCA GATTCTTGAT AATTCTCGTG CTTTAGCAGC AGTTTTAGCT ACTGGAGGGT TGGATGTAGT AACTGGTGGG ACTGATAGCC ATATGGTGGT TGTTGATTTG CGCTCCAAGG GGCTCACTGG CAGGGATGTT TCGTCTTCGT TGGAGAGAGC TGGGATTGTA TGTAATAAAA ATGCTGTTCC ATTTGATACT GAAAAGCCGT GGGTAACCTC TGGTATTAGG TTAGGTGCTG CTGCTGAAAC TTCTAGAGGG TTGGTGGTCA AGGATTTTGA GAAGATAGGG CAGCTTGTAT TGAAGATTGT GGACTCTATG AGGGCTGGAG CGGATATGTC CGTAGTGGAG TCGGGGGTTA GAGAAGAGGT TGCTACTCTT GTTAGGGTAG TGCCGTACGA TACTTTGGCT TGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA