AnphA.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase (IspC)

CENTER ID: AnphA.01136.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01136.a.A1.GE27025 Full length( AnphA.01136.a ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01136.a.A1.PW26996 Full length( AnphA.01136.a ) 1 385
AnphA.01136.a.A1.PW33583 Full length( AnphA.01136.a ) 1 385 unavailable
AnphA.01136.a.A1.PW33583a Full length( AnphA.01136.a ) 1 385 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.469
RefSeq: YP_505043.1
UniProt: Q2GKQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKVSVFGST GFIGKTTVGI LSGNTEDFKV IALVAGSNVS LLAQQAKLLN AEMAVIADDA QYEELKRSLH GSGIRVAAGK EAVLEAAALS VDTAVMAITG IVALSAVMRL IESGVGTIAL ASKESVVCGG VLLRDAACKS GTRIVPVDSE HNAVFRLLSQ GDNPYKITIT ASGGPFLHWS HDQLKSVTIE DALVHPVWKM GKKISVDSAT MMNKALEVLE ASYLFELGYR KIDVVIHPES IVHALAFYQD GTSTALMSLP DMSIPILHAL YWPHRKEVSV REVDLIAYGK LTFIRPDFER FPALKAAFDI LQSSERDVAS IVFNAANEVA VESFLNSEIT FLEIVNIVLH VMNKVPYGKV SSLADIMEYD LLGRCIAREV ISDAS
NT Sequence
GTGAAAAAGG TATCAGTTTT CGGTTCTACA GGATTTATAG GTAAAACTAC TGTAGGTATT TTATCGGGTA ATACAGAAGA TTTTAAGGTT ATTGCGCTTG TAGCTGGTAG TAACGTGAGC TTGTTGGCGC AGCAGGCTAA GTTATTAAAT GCTGAAATGG CGGTTATTGC TGATGATGCT CAATATGAGG AGCTTAAAAG GTCTTTACAT GGTTCAGGTA TAAGGGTTGC TGCTGGTAAG GAGGCTGTGC TTGAAGCGGC TGCCCTTAGT GTAGATACCG CTGTAATGGC TATCACTGGT ATTGTGGCTT TAAGTGCGGT GATGCGGCTG ATAGAAAGTG GTGTAGGTAC TATTGCTTTA GCCAGCAAGG AGAGTGTAGT TTGTGGGGGA GTTCTTCTAA GGGATGCTGC TTGTAAAAGT GGAACTAGGA TTGTTCCGGT GGATTCAGAG CATAATGCGG TTTTTCGTCT ATTATCTCAG GGGGATAATC CCTATAAGAT TACAATTACG GCTTCAGGAG GGCCTTTTCT GCATTGGTCT CATGATCAAT TAAAAAGTGT TACAATAGAA GATGCGCTAG TTCATCCAGT TTGGAAAATG GGTAAGAAAA TCTCTGTGGA TAGTGCAACG ATGATGAATA AAGCTTTGGA AGTATTAGAA GCTAGTTACC TTTTTGAGCT CGGATATAGG AAAATAGATG TTGTGATTCA TCCGGAATCA ATAGTTCATG CTTTGGCGTT TTATCAGGAT GGAACTTCTA CTGCGTTAAT GTCGTTGCCG GATATGAGTA TCCCAATACT TCATGCGTTA TATTGGCCTC ATAGGAAGGA GGTGTCTGTA AGGGAGGTGG ATTTAATTGC GTATGGCAAA TTGACCTTTA TTAGGCCTGA TTTTGAGAGA TTTCCGGCAC TGAAGGCTGC ATTTGATATT TTACAGTCTT CTGAGAGGGA TGTTGCAAGT ATAGTATTTA ATGCTGCAAA TGAAGTTGCG GTGGAGTCAT TTTTAAATTC CGAGATTACT TTTTTAGAGA TAGTTAATAT TGTTTTGCAT GTGATGAACA AGGTTCCTTA TGGAAAAGTC TCTTCTCTAG CAGACATAAT GGAATATGAT TTATTAGGCA GGTGTATAGC TAGAGAGGTT ATTAGCGATG CGTCT
Details for AnphA.01136.a.A1.GE27025
HARVESTED ON: 7/6/2009
SEQUENCED ON: 7/21/2009
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 81
Validated AA Sequence
MAVIADDAQY EELKRSLHGS GIRVAAGKEA VLEAAALSVD TAVMAITGIV ALSAVMRLIE SGVGTIALAS KESVVCGGVL LRDAACKSGT RIVPVDSEHN AVFRLLSQGD NPYKITITAS GGPFLHWSHD QLKSVTIEDA LVHPVWKMGK KISVDSATMM NKALEVLEAS YLFELGYRKI DVVIHPESIV HALAFYQDGT STALMSLPDM SIPILHALYW PHRKEVSVRE VDLIAYGKLT FIRPDFERFP ALKAAFDILQ SSERDVASIV FNAANEVAVE SFLNSEITFL EIVNIVLHVM NKVPYGKVSS LADIMEYDLL GRCIAREVIS DAS
Validated NT Sequence
ttattaagac gcatcgctaa taacctctct agctatacac ctgcctaata aatcatattc cattatgtct gctagagaag agacttttcc ataaggaacc ttgttcatca catgcaaaac aatattaact atctctaaaa aagtaatctc ggaatttaaa aatgactcca ccgcaacttc atttgcagca ttaaatacta tacttgcaac atccctctca gaagactgta aaatatcaaa tgcagccttc agtgccggaa atctctcaaa atcaggccta ataaaggtca atttgccata cgcaattaaa tccacctccc ttacagacac ctccttccta tgaggccaat ataacgcatg aagtattggg atactcatat ccggcaacga cattaacgca gtagaagttc catcctgata aaacgccaaa gcatgaacta ttgattccgg atgaatcaca acatctattt tcctatatcc gagctcaaaa aggtaactag cttctaatac ttccaaagct ttattcatca tcgttgcact atccacagag attttcttac ccattttcca aactggatga actagcgcat cttctattgt aacacttttt aattgatcat gagaccaatg cagaaaaggc cctcctgaag ccgtaattgt aatcttatag ggattatccc cctgagataa tagacgaaaa accgcattat gctctgaatc caccggaaca atcctagttc cacttttaca agcagcatcc cttagaagaa ctcccccaca aactacactc tccttgctgg ctaaagcaat agtacctaca ccactttcta tcagccgcat caccgcactt aaagccacaa taccagtgat agccattaca gcggtatcta cactaagggc agccgcttca agcacagcct ccttaccagc agcaaccctt atacctgaac catgtaaaga ccttttaagc tcctcatatt gagcatcatc agcaataacc gccatttcag catttaataa cttagcctgc tgcgccaaca agctcacgtt actaccagct acaagcgcaa taaccttaaa atcttctgta ttacccgata aaanacctac agtagtttta cctataaatc ctgtagaacc gaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVSVFG STGFIGKTTV GILSGNTEDF KVIALVAGSN VSLLAQQAKL LNAEMAVIAD DAQYEELKRS LHGSGIRVAA GKEAVLEAAA LSVDTAVMAI TGIVALSAVM RLIESGVGTI ALASKESVVC GGVLLRDAAC KSGTRIVPVD SEHNAVFRLL SQGDNPYKIT ITASGGPFLH WSHDQLKSVT IEDALVHPVW KMGKKISVDS ATMMNKALEV LEASYLFELG YRKIDVVIHP ESIVHALAFY QDGTSTALMS LPDMSIPILH ALYWPHRKEV SVREVDLIAY GKLTFIRPDF ERFPALKAAF DILQSSERDV ASIVFNAANE VAVESFLNSE ITFLEIVNIV LHVMNKVPYG KVSSLADIME YDLLGRCIAR EVISDAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaaaag gtatcagttt tcggttctac aggatttata ggtaaaacta ctgtaggtat tttatcgggt aatacagaag attttaaggt tattgcgctt gtagctggta gtaacgtgag cttgttggcg cagcaggcta agttattaaa tgctgaaatg gcggttattg ctgatgatgc tcaatatgag gagcttaaaa ggtctttaca tggttcaggt ataagggttg ctgctggtaa ggaggctgtg cttgaagcgg ctgcccttag tgtagatacc gctgtaatgg ctatcactgg tattgtggct ttaagtgcgg tgatgcggct gatagaaagt ggtgtaggta ctattgcttt agccagcaag gagagtgtag tttgtggggg agttcttcta agggatgctg cttgtaaaag tggaactagg attgttccgg tggattcaga gcataatgcg gtttttcgtc tattatctca gggggataat ccctataaga ttacaattac ggcttcagga gggccttttc tgcattggtc tcatgatcaa ttaaaaagtg ttacaataga agatgcgcta gttcatccag tttggaaaat gggtaagaaa atctctgtgg atagtgcaac gatgatgaat aaagctttgg aagtattaga agctagttac ctttttgagc tcggatatag gaaaatagat gttgtgattc atccggaatc aatagttcat gctttggcgt tttatcagga tggaacttct actgcgttaa tgtcgttgcc ggatatgagt atcccaatac ttcatgcgtt atattggcct cataggaagg aggtgtctgt aagggaggtg gatttaattg cgtatggcaa attgaccttt attaggcctg attttgagag atttccggca ctgaaggctg catttgatat tttacagtct tctgagaggg atgttgcaag tatagtattt aatgctgcaa atgaagttgc ggtggagtca tttttaaatt ccgagattac ttttttagag atagttaata ttgttttgca tgtgatgaac aaggttcctt atggaaaagt ctcttctcta gcagacataa tggaatatga tttattaggc aggtgtatag ctagagaggt tattagcgat gcgtctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for AnphA.01136.a.A1.PW26996
PURIFICATION DATe: 7/28/2009
CONCENTRATION: 25.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 81
Protocol Notes
notes unavailable
Validated AA Sequence
MAVIADDAQY EELKRSLHGS GIRVAAGKEA VLEAAALSVD TAVMAITGIV ALSAVMRLIE SGVGTIALAS KESVVCGGVL LRDAACKSGT RIVPVDSEHN AVFRLLSQGD NPYKITITAS GGPFLHWSHD QLKSVTIEDA LVHPVWKMGK KISVDSATMM NKALEVLEAS YLFELGYRKI DVVIHPESIV HALAFYQDGT STALMSLPDM SIPILHALYW PHRKEVSVRE VDLIAYGKLT FIRPDFERFP ALKAAFDILQ SSERDVASIV FNAANEVAVE SFLNSEITFL EIVNIVLHVM NKVPYGKVSS LADIMEYDLL GRCIAREVIS DAS
Validated NT Sequence
ttattaagac gcatcgctaa taacctctct agctatacac ctgcctaata aatcatattc cattatgtct gctagagaag agacttttcc ataaggaacc ttgttcatca catgcaaaac aatattaact atctctaaaa aagtaatctc ggaatttaaa aatgactcca ccgcaacttc atttgcagca ttaaatacta tacttgcaac atccctctca gaagactgta aaatatcaaa tgcagccttc agtgccggaa atctctcaaa atcaggccta ataaaggtca atttgccata cgcaattaaa tccacctccc ttacagacac ctccttccta tgaggccaat ataacgcatg aagtattggg atactcatat ccggcaacga cattaacgca gtagaagttc catcctgata aaacgccaaa gcatgaacta ttgattccgg atgaatcaca acatctattt tcctatatcc gagctcaaaa aggtaactag cttctaatac ttccaaagct ttattcatca tcgttgcact atccacagag attttcttac ccattttcca aactggatga actagcgcat cttctattgt aacacttttt aattgatcat gagaccaatg cagaaaaggc cctcctgaag ccgtaattgt aatcttatag ggattatccc cctgagataa tagacgaaaa accgcattat gctctgaatc caccggaaca atcctagttc cacttttaca agcagcatcc cttagaagaa ctcccccaca aactacactc tccttgctgg ctaaagcaat agtacctaca ccactttcta tcagccgcat caccgcactt aaagccacaa taccagtgat agccattaca gcggtatcta cactaagggc agccgcttca agcacagcct ccttaccagc agcaaccctt atacctgaac catgtaaaga ccttttaagc tcctcatatt gagcatcatc agcaataacc gccatttcag catttaataa cttagcctgc tgcgccaaca agctcacgtt actaccagct acaagcgcaa taaccttaaa atcttctgta ttacccgata aaanacctac agtagtttta cctataaatc ctgtagaacc gaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVSVFG STGFIGKTTV GILSGNTEDF KVIALVAGSN VSLLAQQAKL LNAEMAVIAD DAQYEELKRS LHGSGIRVAA GKEAVLEAAA LSVDTAVMAI TGIVALSAVM RLIESGVGTI ALASKESVVC GGVLLRDAAC KSGTRIVPVD SEHNAVFRLL SQGDNPYKIT ITASGGPFLH WSHDQLKSVT IEDALVHPVW KMGKKISVDS ATMMNKALEV LEASYLFELG YRKIDVVIHP ESIVHALAFY QDGTSTALMS LPDMSIPILH ALYWPHRKEV SVREVDLIAY GKLTFIRPDF ERFPALKAAF DILQSSERDV ASIVFNAANE VAVESFLNSE ITFLEIVNIV LHVMNKVPYG KVSSLADIME YDLLGRCIAR EVISDAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAAAAG GTATCAGTTT TCGGTTCTAC AGGATTTATA GGTAAAACTA CTGTAGGTAT TTTATCGGGT AATACAGAAG ATTTTAAGGT TATTGCGCTT GTAGCTGGTA GTAACGTGAG CTTGTTGGCG CAGCAGGCTA AGTTATTAAA TGCTGAAATG GCGGTTATTG CTGATGATGC TCAATATGAG GAGCTTAAAA GGTCTTTACA TGGTTCAGGT ATAAGGGTTG CTGCTGGTAA GGAGGCTGTG CTTGAAGCGG CTGCCCTTAG TGTAGATACC GCTGTAATGG CTATCACTGG TATTGTGGCT TTAAGTGCGG TGATGCGGCT GATAGAAAGT GGTGTAGGTA CTATTGCTTT AGCCAGCAAG GAGAGTGTAG TTTGTGGGGG AGTTCTTCTA AGGGATGCTG CTTGTAAAAG TGGAACTAGG ATTGTTCCGG TGGATTCAGA GCATAATGCG GTTTTTCGTC TATTATCTCA GGGGGATAAT CCCTATAAGA TTACAATTAC GGCTTCAGGA GGGCCTTTTC TGCATTGGTC TCATGATCAA TTAAAAAGTG TTACAATAGA AGATGCGCTA GTTCATCCAG TTTGGAAAAT GGGTAAGAAA ATCTCTGTGG ATAGTGCAAC GATGATGAAT AAAGCTTTGG AAGTATTAGA AGCTAGTTAC CTTTTTGAGC TCGGATATAG GAAAATAGAT GTTGTGATTC ATCCGGAATC AATAGTTCAT GCTTTGGCGT TTTATCAGGA TGGAACTTCT ACTGCGTTAA TGTCGTTGCC GGATATGAGT ATCCCAATAC TTCATGCGTT ATATTGGCCT CATAGGAAGG AGGTGTCTGT AAGGGAGGTG GATTTAATTG CGTATGGCAA ATTGACCTTT ATTAGGCCTG ATTTTGAGAG ATTTCCGGCA CTGAAGGCTG CATTTGATAT TTTACAGTCT TCTGAGAGGG ATGTTGCAAG TATAGTATTT AATGCTGCAA ATGAAGTTGC GGTGGAGTCA TTTTTAAATT CCGAGATTAC TTTTTTAGAG ATAGTTAATA TTGTTTTGCA TGTGATGAAC AAGGTTCCTT ATGGAAAAGT CTCTTCTCTA GCAGACATAA TGGAATATGA TTTATTAGGC AGGTGTATAG CTAGAGAGGT TATTAGCGAT GCGTCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for AnphA.01136.a.A1.PW33583
PURIFICATION DATe: 7/2/2011
CONCENTRATION: 40.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 81
Protocol Notes
notes unavailable
Validated AA Sequence
MAVIADDAQY EELKRSLHGS GIRVAAGKEA VLEAAALSVD TAVMAITGIV ALSAVMRLIE SGVGTIALAS KESVVCGGVL LRDAACKSGT RIVPVDSEHN AVFRLLSQGD NPYKITITAS GGPFLHWSHD QLKSVTIEDA LVHPVWKMGK KISVDSATMM NKALEVLEAS YLFELGYRKI DVVIHPESIV HALAFYQDGT STALMSLPDM SIPILHALYW PHRKEVSVRE VDLIAYGKLT FIRPDFERFP ALKAAFDILQ SSERDVASIV FNAANEVAVE SFLNSEITFL EIVNIVLHVM NKVPYGKVSS LADIMEYDLL GRCIAREVIS DAS
Validated NT Sequence
ttattaagac gcatcgctaa taacctctct agctatacac ctgcctaata aatcatattc cattatgtct gctagagaag agacttttcc ataaggaacc ttgttcatca catgcaaaac aatattaact atctctaaaa aagtaatctc ggaatttaaa aatgactcca ccgcaacttc atttgcagca ttaaatacta tacttgcaac atccctctca gaagactgta aaatatcaaa tgcagccttc agtgccggaa atctctcaaa atcaggccta ataaaggtca atttgccata cgcaattaaa tccacctccc ttacagacac ctccttccta tgaggccaat ataacgcatg aagtattggg atactcatat ccggcaacga cattaacgca gtagaagttc catcctgata aaacgccaaa gcatgaacta ttgattccgg atgaatcaca acatctattt tcctatatcc gagctcaaaa aggtaactag cttctaatac ttccaaagct ttattcatca tcgttgcact atccacagag attttcttac ccattttcca aactggatga actagcgcat cttctattgt aacacttttt aattgatcat gagaccaatg cagaaaaggc cctcctgaag ccgtaattgt aatcttatag ggattatccc cctgagataa tagacgaaaa accgcattat gctctgaatc caccggaaca atcctagttc cacttttaca agcagcatcc cttagaagaa ctcccccaca aactacactc tccttgctgg ctaaagcaat agtacctaca ccactttcta tcagccgcat caccgcactt aaagccacaa taccagtgat agccattaca gcggtatcta cactaagggc agccgcttca agcacagcct ccttaccagc agcaaccctt atacctgaac catgtaaaga ccttttaagc tcctcatatt gagcatcatc agcaataacc gccatttcag catttaataa cttagcctgc tgcgccaaca agctcacgtt actaccagct acaagcgcaa taaccttaaa atcttctgta ttacccgata aaanacctac agtagtttta cctataaatc ctgtagaacc gaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVSVFG STGFIGKTTV GILSGNTEDF KVIALVAGSN VSLLAQQAKL LNAEMAVIAD DAQYEELKRS LHGSGIRVAA GKEAVLEAAA LSVDTAVMAI TGIVALSAVM RLIESGVGTI ALASKESVVC GGVLLRDAAC KSGTRIVPVD SEHNAVFRLL SQGDNPYKIT ITASGGPFLH WSHDQLKSVT IEDALVHPVW KMGKKISVDS ATMMNKALEV LEASYLFELG YRKIDVVIHP ESIVHALAFY QDGTSTALMS LPDMSIPILH ALYWPHRKEV SVREVDLIAY GKLTFIRPDF ERFPALKAAF DILQSSERDV ASIVFNAANE VAVESFLNSE ITFLEIVNIV LHVMNKVPYG KVSSLADIME YDLLGRCIAR EVISDAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAAAAG GTATCAGTTT TCGGTTCTAC AGGATTTATA GGTAAAACTA CTGTAGGTAT TTTATCGGGT AATACAGAAG ATTTTAAGGT TATTGCGCTT GTAGCTGGTA GTAACGTGAG CTTGTTGGCG CAGCAGGCTA AGTTATTAAA TGCTGAAATG GCGGTTATTG CTGATGATGC TCAATATGAG GAGCTTAAAA GGTCTTTACA TGGTTCAGGT ATAAGGGTTG CTGCTGGTAA GGAGGCTGTG CTTGAAGCGG CTGCCCTTAG TGTAGATACC GCTGTAATGG CTATCACTGG TATTGTGGCT TTAAGTGCGG TGATGCGGCT GATAGAAAGT GGTGTAGGTA CTATTGCTTT AGCCAGCAAG GAGAGTGTAG TTTGTGGGGG AGTTCTTCTA AGGGATGCTG CTTGTAAAAG TGGAACTAGG ATTGTTCCGG TGGATTCAGA GCATAATGCG GTTTTTCGTC TATTATCTCA GGGGGATAAT CCCTATAAGA TTACAATTAC GGCTTCAGGA GGGCCTTTTC TGCATTGGTC TCATGATCAA TTAAAAAGTG TTACAATAGA AGATGCGCTA GTTCATCCAG TTTGGAAAAT GGGTAAGAAA ATCTCTGTGG ATAGTGCAAC GATGATGAAT AAAGCTTTGG AAGTATTAGA AGCTAGTTAC CTTTTTGAGC TCGGATATAG GAAAATAGAT GTTGTGATTC ATCCGGAATC AATAGTTCAT GCTTTGGCGT TTTATCAGGA TGGAACTTCT ACTGCGTTAA TGTCGTTGCC GGATATGAGT ATCCCAATAC TTCATGCGTT ATATTGGCCT CATAGGAAGG AGGTGTCTGT AAGGGAGGTG GATTTAATTG CGTATGGCAA ATTGACCTTT ATTAGGCCTG ATTTTGAGAG ATTTCCGGCA CTGAAGGCTG CATTTGATAT TTTACAGTCT TCTGAGAGGG ATGTTGCAAG TATAGTATTT AATGCTGCAA ATGAAGTTGC GGTGGAGTCA TTTTTAAATT CCGAGATTAC TTTTTTAGAG ATAGTTAATA TTGTTTTGCA TGTGATGAAC AAGGTTCCTT ATGGAAAAGT CTCTTCTCTA GCAGACATAA TGGAATATGA TTTATTAGGC AGGTGTATAG CTAGAGAGGT TATTAGCGAT GCGTCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for AnphA.01136.a.A1.PW33583a
PURIFICATION DATe: 7/2/2011
CONCENTRATION: 40.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 81
Protocol Notes
notes unavailable
Validated AA Sequence
MAVIADDAQY EELKRSLHGS GIRVAAGKEA VLEAAALSVD TAVMAITGIV ALSAVMRLIE SGVGTIALAS KESVVCGGVL LRDAACKSGT RIVPVDSEHN AVFRLLSQGD NPYKITITAS GGPFLHWSHD QLKSVTIEDA LVHPVWKMGK KISVDSATMM NKALEVLEAS YLFELGYRKI DVVIHPESIV HALAFYQDGT STALMSLPDM SIPILHALYW PHRKEVSVRE VDLIAYGKLT FIRPDFERFP ALKAAFDILQ SSERDVASIV FNAANEVAVE SFLNSEITFL EIVNIVLHVM NKVPYGKVSS LADIMEYDLL GRCIAREVIS DAS
Validated NT Sequence
ttattaagac gcatcgctaa taacctctct agctatacac ctgcctaata aatcatattc cattatgtct gctagagaag agacttttcc ataaggaacc ttgttcatca catgcaaaac aatattaact atctctaaaa aagtaatctc ggaatttaaa aatgactcca ccgcaacttc atttgcagca ttaaatacta tacttgcaac atccctctca gaagactgta aaatatcaaa tgcagccttc agtgccggaa atctctcaaa atcaggccta ataaaggtca atttgccata cgcaattaaa tccacctccc ttacagacac ctccttccta tgaggccaat ataacgcatg aagtattggg atactcatat ccggcaacga cattaacgca gtagaagttc catcctgata aaacgccaaa gcatgaacta ttgattccgg atgaatcaca acatctattt tcctatatcc gagctcaaaa aggtaactag cttctaatac ttccaaagct ttattcatca tcgttgcact atccacagag attttcttac ccattttcca aactggatga actagcgcat cttctattgt aacacttttt aattgatcat gagaccaatg cagaaaaggc cctcctgaag ccgtaattgt aatcttatag ggattatccc cctgagataa tagacgaaaa accgcattat gctctgaatc caccggaaca atcctagttc cacttttaca agcagcatcc cttagaagaa ctcccccaca aactacactc tccttgctgg ctaaagcaat agtacctaca ccactttcta tcagccgcat caccgcactt aaagccacaa taccagtgat agccattaca gcggtatcta cactaagggc agccgcttca agcacagcct ccttaccagc agcaaccctt atacctgaac catgtaaaga ccttttaagc tcctcatatt gagcatcatc agcaataacc gccatttcag catttaataa cttagcctgc tgcgccaaca agctcacgtt actaccagct acaagcgcaa taaccttaaa atcttctgta ttacccgata aaanacctac agtagtttta cctataaatc ctgtagaacc gaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKVSVFG STGFIGKTTV GILSGNTEDF KVIALVAGSN VSLLAQQAKL LNAEMAVIAD DAQYEELKRS LHGSGIRVAA GKEAVLEAAA LSVDTAVMAI TGIVALSAVM RLIESGVGTI ALASKESVVC GGVLLRDAAC KSGTRIVPVD SEHNAVFRLL SQGDNPYKIT ITASGGPFLH WSHDQLKSVT IEDALVHPVW KMGKKISVDS ATMMNKALEV LEASYLFELG YRKIDVVIHP ESIVHALAFY QDGTSTALMS LPDMSIPILH ALYWPHRKEV SVREVDLIAY GKLTFIRPDF ERFPALKAAF DILQSSERDV ASIVFNAANE VAVESFLNSE ITFLEIVNIV LHVMNKVPYG KVSSLADIME YDLLGRCIAR EVISDAS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAAAAG GTATCAGTTT TCGGTTCTAC AGGATTTATA GGTAAAACTA CTGTAGGTAT TTTATCGGGT AATACAGAAG ATTTTAAGGT TATTGCGCTT GTAGCTGGTA GTAACGTGAG CTTGTTGGCG CAGCAGGCTA AGTTATTAAA TGCTGAAATG GCGGTTATTG CTGATGATGC TCAATATGAG GAGCTTAAAA GGTCTTTACA TGGTTCAGGT ATAAGGGTTG CTGCTGGTAA GGAGGCTGTG CTTGAAGCGG CTGCCCTTAG TGTAGATACC GCTGTAATGG CTATCACTGG TATTGTGGCT TTAAGTGCGG TGATGCGGCT GATAGAAAGT GGTGTAGGTA CTATTGCTTT AGCCAGCAAG GAGAGTGTAG TTTGTGGGGG AGTTCTTCTA AGGGATGCTG CTTGTAAAAG TGGAACTAGG ATTGTTCCGG TGGATTCAGA GCATAATGCG GTTTTTCGTC TATTATCTCA GGGGGATAAT CCCTATAAGA TTACAATTAC GGCTTCAGGA GGGCCTTTTC TGCATTGGTC TCATGATCAA TTAAAAAGTG TTACAATAGA AGATGCGCTA GTTCATCCAG TTTGGAAAAT GGGTAAGAAA ATCTCTGTGG ATAGTGCAAC GATGATGAAT AAAGCTTTGG AAGTATTAGA AGCTAGTTAC CTTTTTGAGC TCGGATATAG GAAAATAGAT GTTGTGATTC ATCCGGAATC AATAGTTCAT GCTTTGGCGT TTTATCAGGA TGGAACTTCT ACTGCGTTAA TGTCGTTGCC GGATATGAGT ATCCCAATAC TTCATGCGTT ATATTGGCCT CATAGGAAGG AGGTGTCTGT AAGGGAGGTG GATTTAATTG CGTATGGCAA ATTGACCTTT ATTAGGCCTG ATTTTGAGAG ATTTCCGGCA CTGAAGGCTG CATTTGATAT TTTACAGTCT TCTGAGAGGG ATGTTGCAAG TATAGTATTT AATGCTGCAA ATGAAGTTGC GGTGGAGTCA TTTTTAAATT CCGAGATTAC TTTTTTAGAG ATAGTTAATA TTGTTTTGCA TGTGATGAAC AAGGTTCCTT ATGGAAAAGT CTCTTCTCTA GCAGACATAA TGGAATATGA TTTATTAGGC AGGTGTATAG CTAGAGAGGT TATTAGCGAT GCGTCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA