SSGCID: Target Page

AnphA.01122.a

Ribosomal RNA small subunit methyltransferase A

CENTER ID:	AnphA.01122.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01122.a.A2.GE30740	Mature protein( AnphA.01122.a )		23	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.673
RefSeq:	YP_505210.1
UniProt:	Q2GK91

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKITKYRTKK SLGQCFILDP AIAEKIVSYA GCLEQYNVIE VGPGLGIMTQ SILNKEVRRL TAIEKDRRLS NIHSKLKEAH AEYDCIFEDI LDVNIEQLLS QSPLKMISNL PYNISVILLL KLLPYIHRFE KLILMFQKEV ADRIVAQPNT KSYSILSILV QLLCDVRKVE DFPPEIFSPS PKVYSSVIEI TPLLSPRFSV DNSYFAQVLK KLFHCRRKTI RNSLKSCIKD ADALFIGCNI DPNARAESLT IEQLCSLTNA LKARNINII

NT Sequence

ATGAAAATTA CTAAATACAG GACGAAAAAA TCTTTAGGGC AGTGTTTCAT ACTAGATCCT GCTATCGCGG AAAAAATTGT TTCATATGCA GGATGCTTGG AACAATATAA CGTAATAGAA GTCGGACCAG GGCTTGGAAT AATGACTCAA TCAATCCTTA ACAAAGAGGT ACGTAGACTA ACCGCTATTG AGAAGGATAG AAGATTATCA AACATACACA GCAAACTAAA AGAAGCACAT GCTGAATATG ATTGCATCTT TGAAGATATT TTGGATGTCA ACATCGAACA GCTGCTATCG CAATCGCCCC TCAAAATGAT ATCCAACCTC CCGTACAATA TATCTGTAAT TTTGCTTCTA AAACTCCTTC CCTATATACA TCGCTTCGAG AAGCTCATTC TTATGTTCCA AAAAGAAGTA GCTGATAGGA TCGTAGCTCA GCCAAATACG AAAAGCTATT CTATTCTGTC TATTCTTGTG CAGCTGCTAT GCGATGTTAG AAAAGTTGAA GATTTCCCAC CAGAAATTTT CTCCCCATCT CCTAAAGTAT ATTCTTCAGT TATAGAGATA ACTCCCTTAT TATCTCCTAG ATTCTCTGTA GATAATTCAT ACTTTGCACA AGTATTAAAA AAGCTATTCC ACTGCCGACG AAAAACAATT CGCAACTCGT TAAAGTCATG TATAAAAGAT GCTGATGCAT TATTTATTGG ATGTAATATT GACCCTAATG CAAGAGCAGA ATCCCTGACT ATAGAGCAAT TGTGCTCACT AACAAATGCC TTAAAAGCGC GCAATATCAA TATAATC

Details for AnphA.01122.a.A2.GE30740

HARVESTED ON:	11/9/2010
SEQUENCED ON:	11/15/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAEKIVSYA GCLEQYNVIE VGPGLGIMTQ SILNKEVRRL TAIEKDRRLS NIHSKLKEAH AEYDCIFEDI LDVNIEQLLS QSPLKMISNL PYNISVILLL KLLPYIHRFE KLILMFQKEV ADRIVAQPNT KSYSILSILV QLLCDVRKVE DFPPEIFSPS PKVYSSVIEI TPLLSPRFSV DNSYFAQVLK KLFHCRRKTI RNSLKSCIKD ADALFIGCNI DPNARXESLT IEQLCSLTNA LKARNINII

Validated NT Sequence

tttgtttact ttaagangga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcggaa aaaattgttt catatgcagg atgcttggaa caatataacg taatagaagt cggaccaggg cttggaataa tgactcaatc aatccttaac aaagaggtac gtagactaac cgctattgag aaggatagaa gattatcaaa catacacagc aaactaaaag aagcacatgc tgaatatgat tgcatctttg aagatatttt ggatgtcaac atcgaacagc tgctatcgca atcgcccctc aaaatgatat ccaacctccc gtacaatata tctgtaattt tgcttctaaa actccttccc tatatacatc gcttcgagaa gctcattctt atgttccaaa aagaagtagc tgataggatc gtagctcagc caaatacgaa aagctattct attctgtcta ttcttgtgca gctgctatgc gatgttagaa aagttgaaga tttcccacca gaaattttct ccccatctcc taaagtatat tcttcagtta tagagataac tcccttatta tctcctagat tctctgtaga taattcatac tttgcacaag tattaaaaaa gctattccac tgccgacgaa aaacaattcg caactcgtta aagtcatgta taaaagatgc tgatgcatta tttattggat gtaatattga ccctaatgca agancagaat ccctgactat agagcaattg tgctcactaa caaatgcctt aaaagcgcgc aatatcaata taatctaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaanct gagttggctg ctgccnnnnn nnnnnnnaaa a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggaaaaa attgtttcat atgcaggatg cttggaacaa tataacgtaa tagaagtcgg accagggctt ggaataatga ctcaatcaat ccttaacaaa gaggtacgta gactaaccgc tattgagaag gatagaagat tatcaaacat acacagcaaa ctaaaagaag cacatgctga atatgattgc atctttgaag atattttgga tgtcaacatc gaacagctgc tatcgcaatc gcccctcaaa atgatatcca acctcccgta caatatatct gtaattttgc ttctaaaact ccttccctat atacatcgct tcgagaagct cattcttatg ttccaaaaag aagtagctga taggatcgta gctcagccaa atacgaaaag ctattctatt ctgtctattc ttgtgcagct gctatgcgat gttagaaaag ttgaagattt cccaccagaa attttctccc catctcctaa agtatattct tcagttatag agataactcc cttattatct cctagattct ctgtagataa ttcatacttt gcacaagtat taaaaaagct attccactgc cgacgaaaaa caattcgcaa ctcgttaaag tcatgtataa aagatgctga tgcattattt attggatgta atattgaccc taatgcaaga gcagaatccc tgactataga gcaattgtgc tcactaacaa atgccttaaa agcgcgcaat atcaatataa tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa