SSGCID: Target Page

AnphA.01115.a

dethiobiotin synthetase

CENTER ID:	AnphA.01115.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01115.a.A1.GE27022	Full length( AnphA.01115.a )		1	225

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1320
RefSeq:	YP_505758.1
UniProt:	Q2GIP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPAYFITSTG TDVGKTFVTT ALCWHFRRIG HNVQAIKPVI TGWNSATEGE SDTVKIIQSL ECTVSEQSIS RVSPWRFEAP RAPNVAADMS GAHLDYEEIL RFCRKSSQTL DSYTFIEGVG GVMSPITENK SCLELIHDLG IDVVLVIGSY LGSISHTLTA MKVLQGLRIH VILSEKEEEN PCARDVVEYV NQYYNCTVYI QEHVSRNLRA PWTHTSPEIA HFLLR

NT Sequence

GTGCCAGCAT ATTTCATTAC GTCCACAGGG ACAGACGTCG GAAAAACCTT CGTGACCACA GCATTGTGCT GGCATTTTAG AAGAATTGGC CACAACGTGC AAGCTATAAA GCCTGTAATT ACGGGATGGA ACAGCGCTAC AGAGGGTGAG TCCGATACGG TAAAAATCAT CCAAAGTTTA GAATGCACAG TATCGGAACA AAGCATATCA AGAGTCTCAC CATGGCGGTT TGAAGCACCT CGTGCACCTA ATGTGGCTGC CGATATGTCT GGCGCACACT TAGATTATGA AGAGATTCTG CGGTTTTGTC GCAAAAGCTC TCAAACACTT GACAGCTATA CATTTATTGA GGGCGTTGGT GGAGTGATGT CCCCGATTAC CGAGAACAAA AGCTGCCTTG AACTAATACA TGATTTAGGA ATAGACGTGG TACTGGTGAT CGGATCATAT CTTGGGAGTA TTAGTCATAC ACTGACTGCT ATGAAAGTTT TGCAAGGCCT TCGAATACAC GTCATTTTGT CTGAGAAAGA GGAAGAGAAC CCATGCGCCC GTGATGTGGT AGAATACGTG AACCAGTATT ACAACTGCAC AGTTTATATA CAGGAACATG TAAGCAGAAA TTTACGCGCT CCATGGACGC ATACTTCCCC AGAGATAGCA CATTTTCTTT TAAGG

Details for AnphA.01115.a.A1.GE27022

HARVESTED ON:	7/6/2009
SEQUENCED ON:	7/21/2009
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVPAYFITS TGTDVGKTFV TTALCWHFRR IGHNVQAIKP VITGWNSATE GESDTVKIIQ SLECTVSEQS ISRVSPWRFE APRAPNVAAD MSGAHLDYEE ILRFCRKSSQ TLDSYTFIEG VGGVMSPITE NKSCLELIHD LGIDVVLVIG SYLGSISHTL TAMKVLQGLR IHVILSEKEE ENPCARDVVE YVNQYYNCTV YIQEHVSRNL RAPWTHTSPE IAHFLLR

Validated NT Sequence

ttaanaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgcca gcatatttca ttacgtccac agggacagac gtcggaaaaa ccttcgtgac cacagcattg tgctggcatt ttagaagaat tggccacaac gtgcaagcta taaagcctgt aattacggga tggaacagcg ctacagaggg tgagtccgat acggtaaaaa tcatccaaag tttagaatgc acagtatcgg aacaaagcat atcaagagtc tcaccatggc ggtttgaagc acctcgtgca cctaatgtgg ctgccgatat gtctggcgca cacttagatt atgaagagat tctgcggttt tgtcgcaaaa gctctcaaac acttgacagc tatacattta ttgagggcgt tggtggagtg atgtccccga ttaccgagaa caaaagctgc cttgaactaa tacatgattt aggaatagac gtggtactgg tgatcggatc atatcttggg agtattagtc atacactgac tgctatgaaa gttttgcaag gccttcgaat acacgtcatt ttgtctgaga aagaggaaga gaacccatgc gcccgtgatg tggtagaata cgtgaaccag tattacaact gcacagttta tatacaggaa catgtaagca gaaatttacg cgctccatgg acgcatactt ccccagagat agcacatttt cttttaaggt aataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccagca tatttcatta cgtccacagg gacagacgtc ggaaaaacct tcgtgaccac agcattgtgc tggcatttta gaagaattgg ccacaacgtg caagctataa agcctgtaat tacgggatgg aacagcgcta cagagggtga gtccgatacg gtaaaaatca tccaaagttt agaatgcaca gtatcggaac aaagcatatc aagagtctca ccatggcggt ttgaagcacc tcgtgcacct aatgtggctg ccgatatgtc tggcgcacac ttagattatg aagagattct gcggttttgt cgcaaaagct ctcaaacact tgacagctat acatttattg agggcgttgg tggagtgatg tccccgatta ccgagaacaa aagctgcctt gaactaatac atgatttagg aatagacgtg gtactggtga tcggatcata tcttgggagt attagtcata cactgactgc tatgaaagtt ttgcaaggcc ttcgaataca cgtcattttg tctgagaaag aggaagagaa cccatgcgcc cgtgatgtgg tagaatacgt gaaccagtat tacaactgca cagtttatat acaggaacat gtaagcagaa atttacgcgc tccatggacg catacttccc cagagatagc acattttctt ttaaggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa