AnphA.01110.a

4-diphosphocytidyl-2C-methyl-D-erythritol kinase (IspE)

CENTER ID:	AnphA.01110.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01110.a.A1.GE27021	Full length( AnphA.01110.a )		1	296

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130885
PATRIC ID:	fig\|212042.8.peg.623
RefSeq:	YP_505165.1
UniProt:	Q2GKD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSKYFISAPA KINLFLHIVG KAPCGYHLIE SVFAFVELYD VLEFDIGSRN RGIRFLKPSC INRRDNTIQR AIGHLVRRCS PGTTDNVYVK VLKNIPVSSG LAGGSADAAA AINLLSKLWG INEKETERVA FRVGSDVPVC LESKTAFVAG MGDVVELLED SFLPRHVILV GPKVELSTKS VFDMYNPKAF SPSIGKLPGN SEDWLSLLKE ARNDLTEVSV ELVPEIRIIL DVLGSLDGCC FSRMSGSGAM SFAIFEDENS AELATRYLRR NYPDWFIFKT RIIRSSDQED NNMGNL

NT Sequence

ATGTCGAAGT ATTTTATTAG CGCGCCTGCG AAGATAAACT TGTTTCTCCA TATAGTCGGT AAAGCTCCGT GTGGGTATCA CTTAATAGAG TCTGTCTTTG CATTTGTAGA GTTATACGAT GTTTTAGAGT TTGACATTGG CTCTAGAAAC CGAGGGATAA GGTTTCTCAA ACCTTCGTGT ATTAATAGAC GTGATAACAC TATACAGCGA GCTATTGGAC ATTTGGTGAG GAGATGCTCG CCGGGCACTA CGGATAATGT TTATGTTAAA GTTTTGAAGA ATATTCCTGT ATCGTCTGGA TTGGCAGGCG GGTCAGCAGA TGCCGCTGCT GCTATTAATC TGCTAAGCAA GCTGTGGGGC ATAAATGAAA AAGAAACTGA GAGGGTAGCT TTTAGGGTTG GAAGCGATGT TCCTGTGTGT TTAGAATCTA AAACTGCGTT TGTTGCGGGC ATGGGAGACG TAGTGGAATT GCTAGAAGAC TCATTCTTGC CTAGGCACGT TATTTTGGTA GGGCCCAAAG TGGAATTGAG CACAAAAAGT GTCTTTGACA TGTACAATCC CAAAGCGTTT TCTCCAAGTA TAGGTAAGTT GCCTGGTAAT TCTGAGGATT GGCTTTCTTT ATTGAAGGAA GCTCGTAATG ACTTAACGGA GGTTTCTGTA GAATTGGTAC CGGAGATTCG TATCATATTG GATGTGCTGG GTTCATTAGA TGGGTGTTGC TTTTCCAGAA TGAGTGGAAG TGGGGCTATG AGCTTCGCTA TTTTTGAGGA TGAGAATTCA GCTGAGTTAG CCACTAGGTA TCTGAGGAGA AATTACCCAG ACTGGTTTAT TTTCAAAACT AGGATTATTA GGTCGAGTGA TCAGGAAGAT AATAATATGG GTAATCTT

Details for AnphA.01110.a.A1.GE27021

HARVESTED ON:	7/6/2009
SEQUENCED ON:	7/21/2009
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMSKYFIS APAKINLFLH IVGKAPCGYH LIESVFAFVE LYDVLEFDIG SRNRGIRFLK PSCINRRDNT IQRAIGHLVR RCSPGTTDNA YVKVLKNIPV SSGLAGGSAD AAAAINLLSK LWGINEKETE RVAFRVGSDV PVCLESKTAF VAGMGXVVEL LEDSFLPRHV ILVGPKVELS TKSVFDMYNP KAFSPSIGKL PGNSEDWLSL LKEARNDLTE VSVELVPEIR IILDVLGSLD GCCFSRMSGS GAMSFAIFED ENSAELATRY LRRNYPDWFI FKTRIIRSSD QEDNNMG

Validated NT Sequence

tacccatatt attatcttcc tgatcactcg acctaataat cctagttttg aaaataaacc agtctgggta atttctcctc agatacctag tggctaactc agctgaattc tcatcctcaa aaatagcgaa gctcatagcc ccacttccac tcattctgga aaagcaacac ccatctaatg aacccagcac atccaatatg atacgaatct ccggtaccaa ttctacagaa acctccgtta agtcattacg agcttccttc aataaagaaa gccaatcctc agaattacca ggcaacttac ctatacttgg agaaaacgct ttgggattgt acatgtcaaa gacacttttt gtgctcaatt ccactttggg ccctaccaaa ataacgtgcc taggcaagaa tgagtcttct agcaattcca ctacntctcc catgcccgca acaaacgcag ttttagattc taaacacaca ggaacatcgc ttccaaccct aaaagctacc ctctcagttt ctttttcatt tatgccccac agcttgctta gcagattaat agcagcagcg gcatctgctg acccgcctgc caatccagac gatacaggaa tattcttcaa aactttaaca taagcattat ccgtagtgcc cggcgagcat ctcctcacca aatgtccaat agctcgctgt atagtgttat cacgtctatt aatacacgaa ggtttgagaa accttatccc tcggtttcta gagccaatgt caaactctaa aacatcgtat aactctacaa atgcaaagac agactctatt aagtgatacc cacacggagc tttaccgact atatggagaa acaagtttat cttcgcaggc gcgctaataa aatacttcga catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSKYFISAP AKINLFLHIV GKAPCGYHLI ESVFAFVELY DVLEFDIGSR NRGIRFLKPS CINRRDNTIQ RAIGHLVRRC SPGTTDNVYV KVLKNIPVSS GLAGGSADAA AAINLLSKLW GINEKETERV AFRVGSDVPV CLESKTAFVA GMGDVVELLE DSFLPRHVIL VGPKVELSTK SVFDMYNPKA FSPSIGKLPG NSEDWLSLLK EARNDLTEVS VELVPEIRII LDVLGSLDGC CFSRMSGSGA MSFAIFEDEN SAELATRYLR RNYPDWFIFK TRIIRSSDQE DNNMGNL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgaagtat tttattagcg cgcctgcgaa gataaacttg tttctccata tagtcggtaa agctccgtgt gggtatcact taatagagtc tgtctttgca tttgtagagt tatacgatgt tttagagttt gacattggct ctagaaaccg agggataagg tttctcaaac cttcgtgtat taatagacgt gataacacta tacagcgagc tattggacat ttggtgagga gatgctcgcc gggcactacg gataatgttt atgttaaagt tttgaagaat attcctgtat cgtctggatt ggcaggcggg tcagcagatg ccgctgctgc tattaatctg ctaagcaagc tgtggggcat aaatgaaaaa gaaactgaga gggtagcttt tagggttgga agcgatgttc ctgtgtgttt agaatctaaa actgcgtttg ttgcgggcat gggagacgta gtggaattgc tagaagactc attcttgcct aggcacgtta ttttggtagg gcccaaagtg gaattgagca caaaaagtgt ctttgacatg tacaatccca aagcgttttc tccaagtata ggtaagttgc ctggtaattc tgaggattgg ctttctttat tgaaggaagc tcgtaatgac ttaacggagg tttctgtaga attggtaccg gagattcgta tcatattgga tgtgctgggt tcattagatg ggtgttgctt ttccagaatg agtggaagtg gggctatgag cttcgctatt tttgaggatg agaattcagc tgagttagcc actaggtatc tgaggagaaa ttacccagac tggtttattt tcaaaactag gattattagg tcgagtgatc aggaagataa taatatgggt aatcttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa