SSGCID: Target Page

AnphA.01094.a

Holliday junction DNA helicase B

CENTER ID:	AnphA.01094.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01094.a.MB1.GE30418	Full length( AnphA.01094.a )		1	329

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.176
RefSeq:	YP_504790.1
UniProt:	Q2GLG1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNELLHQHKA IPEDERNFAL RPSLIEEFVG QSEIIENLKV FIKSAYERRA TLDHVLLYGP PGLGKTTLAH IIAKELKVSL RSTSGPLLSK AGDLAAILTN LQPMDVLFID EIHRLHRNIE EILYSAMEDC CLDIVVGEGC GARTLRIDLP AFTLVGATTR FGLISNPLRD RFGIPLHLEF YSVEELMLVI KRAAHVICTD IDDSGAYEIA SRSRGTPRIA LRLFRRVRDF MVVERQSIID NHFADSALFN LGVDKSGLDK MDIKYLSFIY EAKNAVGIET IAAALSEDVG NIEETIEPYL LKIGFIQRTP RGRILTTKAI EHLMNYKYI

NT Sequence

GTGAATGAGC TTTTACATCA ACACAAAGCA ATACCTGAAG ATGAACGTAA TTTTGCATTA CGTCCGAGCT TGATCGAGGA ATTTGTCGGG CAGAGTGAGA TAATAGAGAA TCTCAAGGTA TTCATCAAGT CAGCCTATGA AAGAAGAGCA ACACTGGACC ATGTGCTGTT ATATGGGCCC CCAGGGCTGG GCAAAACTAC ACTAGCGCAT ATAATCGCCA AGGAACTTAA AGTGAGCTTG AGGTCAACTT CCGGACCTCT TTTGAGTAAA GCTGGAGATC TTGCCGCTAT ACTCACGAAC CTACAGCCCA TGGATGTGCT ATTTATAGAC GAGATACATA GACTACACAG AAACATCGAA GAAATCTTAT ACTCCGCCAT GGAAGACTGC TGTCTTGATA TTGTTGTCGG TGAAGGCTGC GGTGCAAGAA CGCTGCGCAT TGATCTCCCC GCTTTTACTT TAGTAGGCGC AACGACAAGA TTTGGCTTAA TATCCAACCC TCTTAGAGAT AGGTTCGGTA TTCCGTTGCA TCTAGAATTC TACTCTGTAG AAGAGTTAAT GCTGGTGATA AAGCGAGCCG CACATGTGAT TTGTACCGAC ATTGATGATA GCGGCGCTTA TGAGATTGCC TCACGTTCTA GAGGTACTCC GAGAATTGCA CTTCGATTAT TTAGGAGAGT GCGTGATTTC ATGGTTGTGG AAAGACAGAG TATAATAGAC AATCATTTTG CTGATTCTGC ACTTTTCAAC CTTGGTGTAG ATAAATCTGG TCTCGATAAG ATGGATATCA AGTATCTCAG CTTCATCTAT GAGGCAAAAA ACGCAGTCGG AATAGAGACT ATTGCTGCTG CCCTTTCAGA AGATGTAGGT AATATTGAAG AGACCATTGA ACCGTATCTA CTGAAAATAG GGTTCATACA ACGCACACCT CGTGGAAGAA TTCTTACCAC AAAGGCCATT GAGCACTTAA TGAACTATAA ATATATC

Details for AnphA.01094.a.MB1.GE30418

HARVESTED ON:	5/11/2010
SEQUENCED ON:	10/7/2010
EXPECTED MW:	82kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	29
PERCENT COVERAGE:	10

Validated AA Sequence

MAEYKISSMF LCSLCISSIN STSMGCRFVS IAARSPALLK RGPEVDLKLT LSSLAIICAS VVLXSPGGPY NSTWSSVALL S

Validated NT Sequence

anacccnggg nnnggntcga ngnngnanga gcttttncat caacacaaag caatacctga agatgaanat tttgcnttac gtccgagctt gaccgaggaa tttgtcgggc agagngagat aatagagaat ctcaaggtat nnnnnaagtc agcctatgaa agaagagcaa cactggacca tgtgctgtta tatgggcccc cagggctggn caaaactaca ctagcgcata taatcgccaa ggaacttaaa gtgagcttga ggtcaacttc cggacctctt ttgagtaaag ctggagatct tgccgctata ctcacgaacc tacagcccat ggatgtgcta tttatagacg agatacatag actacacaga aacatcgaag aaatcttata ctccgccatg gaagactgct gtcttgnatt gttgtcggng aaggctgcgg tgcaagaacg ctgcgcattg atctccccgc ttttacttta gtaggcgcna cgacaagatn tgnntnanna tccaaccctc ttagagatag gntcggtatt ccgttgcatc tagaantcta ctctgtagaa gagntaatgc tggngataaa gcgagccgca catgtgantt ngtaccgaca tngatgatag cggcgcttat gagattnnct cacgnnctag aggnactccg agaattgcac ttcgatnatt taggagagtn ngtgatttca tgnngtggaa agacanagta tantagannt cattttgcng antctgcact tttcacnttg ntnnanannn nnnnnnntcn ata

Expected Protein Sequence

MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MVNELLHQHK AIPEDERNFA LRPSLIEEFV GQSEIIENLK VFIKSAYERR ATLDHVLLYG PPGLGKTTLA HIIAKELKVS LRSTSGPLLS KAGDLAAILT NLQPMDVLFI DEIHRLHRNI EEILYSAMED CCLDIVVGEG CGARTLRIDL PAFTLVGATT RFGLISNPLR DRFGIPLHLE FYSVEELMLV IKRAAHVICT DIDDSGAYEI ASRSRGTPRI ALRLFRRVRD FMVVERQSII DNHFADSALF NLGVDKSGLD KMDIKYLSFI YEAKNAVGIE TIAAALSEDV GNIEETIEPY LLKIGFIQRT PRGRILTTKA IEHLMNYKYI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggt gaatgagctt ttacatcaac acaaagcaat acctgaagat gaacgtaatt ttgcattacg tccgagcttg atcgaggaat ttgtcgggca gagtgagata atagagaatc tcaaggtatt catcaagtca gcctatgaaa gaagagcaac actggaccat gtgctgttat atgggccccc agggctgggc aaaactacac tagcgcatat aatcgccaag gaacttaaag tgagcttgag gtcaacttcc ggacctcttt tgagtaaagc tggagatctt gccgctatac tcacgaacct acagcccatg gatgtgctat ttatagacga gatacataga ctacacagaa acatcgaaga aatcttatac tccgccatgg aagactgctg tcttgatatt gttgtcggtg aaggctgcgg tgcaagaacg ctgcgcattg atctccccgc ttttacttta gtaggcgcaa cgacaagatt tggcttaata tccaaccctc ttagagatag gttcggtatt ccgttgcatc tagaattcta ctctgtagaa gagttaatgc tggtgataaa gcgagccgca catgtgattt gtaccgacat tgatgatagc ggcgcttatg agattgcctc acgttctaga ggtactccga gaattgcact tcgattattt aggagagtgc gtgatttcat ggttgtggaa agacagagta taatagacaa tcattttgct gattctgcac ttttcaacct tggtgtagat aaatctggtc tcgataagat ggatatcaag tatctcagct tcatctatga ggcaaaaaac gcagtcggaa tagagactat tgctgctgcc ctttcagaag atgtaggtaa tattgaagag accattgaac cgtatctact gaaaataggg ttcatacaac gcacacctcg tggaagaatt cttaccacaa aggccattga gcacttaatg aactataaat atatctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa