AnphA.01080.a

S-adenosyl-methyltransferase MraW

CENTER ID: AnphA.01080.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01080.a.A1.GE27036 Full length( AnphA.01080.a ) 1 295
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.745
RefSeq: YP_505272.1
UniProt: Q2GK29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDSHRPVLLN EMLELLSPKD GAVYVDATFG GGGYSRGILE RAKCTVLAID QDPIASEFYQ SLHSQFPGRV HFFLSKFSRL REALQHFDHN QVDGVVFDVG VSSMQLSDPD RGFSFMKEGP LDMRMDMLRS QSKSAATFVN SLSEKDMADV IFYYGGERLS RRVTRAIISA RESNNKIRNT SDLAKIIRSV VPRSKANPID PATRTFQAIR IWVNDELEEL KAGLEAASQI VGVGGKIIVT SFHSLEDRIV KHKFNALREK GFQLINKKAI KPTEKEISEN TRSRSAKLRG IVKEE
NT Sequence
ATGGATTCTC ATAGACCTGT ACTACTAAAC GAAATGCTAG AGCTTCTTTC TCCTAAAGAC GGTGCAGTAT ATGTTGACGC AACGTTTGGT GGAGGCGGCT ATAGTAGGGG AATATTGGAA CGGGCTAAAT GTACTGTACT AGCTATTGAT CAAGATCCTA TTGCTTCAGA ATTCTATCAA TCTTTACATT CGCAATTCCC TGGACGGGTG CACTTTTTTC TCAGCAAGTT CAGTAGGCTT CGAGAGGCGT TACAGCATTT TGATCACAAT CAAGTAGATG GTGTTGTATT TGATGTTGGA GTATCAAGTA TGCAGCTGAG TGATCCAGAC AGGGGATTCT CATTTATGAA GGAAGGGCCA CTTGATATGA GAATGGATAT GTTGCGCTCT CAATCCAAAA GCGCCGCGAC TTTTGTAAAT TCTTTATCAG AAAAAGACAT GGCAGATGTA ATCTTCTATT ACGGAGGAGA GAGGCTTTCA AGACGCGTTA CCAGAGCTAT AATCAGCGCT AGAGAGAGTA ATAATAAGAT ACGTAACACG AGTGACTTGG CAAAAATTAT CAGGTCTGTT GTGCCCCGCA GTAAGGCAAA TCCAATAGAT CCGGCAACAA GGACCTTTCA GGCTATCAGA ATATGGGTAA ATGATGAGCT AGAAGAGCTA AAAGCAGGTC TAGAAGCTGC TTCTCAAATT GTAGGAGTTG GAGGCAAGAT TATTGTTACT TCATTTCATT CACTCGAGGA TAGGATAGTA AAACATAAGT TCAACGCTCT ACGAGAGAAG GGATTTCAAC TTATCAATAA AAAAGCTATA AAGCCCACTG AAAAAGAAAT TAGCGAAAAT ACTAGGTCGC GATCAGCAAA ATTGCGTGGC ATAGTAAAAG AGGAG
Details for AnphA.01080.a.A1.GE27036
HARVESTED ON: 7/6/2009
SEQUENCED ON: 7/21/2009
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 96
Validated AA Sequence
MXXLEAQTQG PGSMDSHRPV LLNEMLELLS PKDGAVYVDA TFGGGGYSRG ILERAKCTVL AIDQDPIASE FYQSLHSQFP GRVHFFLSKF SRLREALQHF DHNQVDGVVF DVGVSSMQLS DPDRGFSFMK EGPLDMRMDM LRSQSKSAAT FVNSLSEKDM ADVIFYYGGE RLSRRVTRAI ISARESNDKI RNTSDLAKII RSVVPRSKAN PIDPATRTFQ AIRIWVNDEL EELKAGLEAA SQIVGVGGKI IVTSFHSLED RIVKHKFNAL REKGLQLINK KAIKPTEKEI SENTRSRSAK LRGIVK
Validated NT Sequence
cttttactat gccacgcaat tttgctgatc gcgacctagt attttcgcta atttcttttt cagtgggctt tatagctttt ttattgataa gttgaagtcc cttctctcgt agagcgttga acttatgttt tactatccta tcctcgagtg aatgaaatga agtaacaata atcttgcctc caactcctac aatttgagaa gcagcttcta gacctgcttt tagctcttct agctcatcat ttacccatat tctgatagcc tgaaaggtcc ttgttgccgg atctattgga tttgccttac tgcggggcac aacagacctg ataatttttg ccaagtcact cgtgttacgt atcttatcat tactctctct agcgctgatt atagctctgg taacgcgtct tgaaagcctc tctcctccgt aatagaagat tacatctgcc atgtcttttt ctgataaaga atttacaaaa gtcgcggcgc ttttggattg agagcgcaac atatccattc tcatatcaag tggcccttcc ttcataaatg agaatcccct gtctggatca ctcagctgca tacttgatac tccaacatca aatacaacac catctacttg attgtgatca aaatgctgta acgcctctcg aagcctactg aacttgctga gaaaaaagtg cacccgtcca gggaattgcg aatgtaaaga ttgatagaat tctgaagcaa taggatcttg atcaatagct agtacagtac atttagcccg ttccaatatt cccctactat agccgcctcc accaaacgtt gcgtcaacat atactgcacc gtctttagga gaaagaagct ctagcatttc gtttagtagt acaggtctat gagaatccat cgaaccagga ccctgggtct gagcttccag ggnnnccata tgatggtgan ggtgatgagc canggtatnn ntccttcttn a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDSHRPVLL NEMLELLSPK DGAVYVDATF GGGGYSRGIL ERAKCTVLAI DQDPIASEFY QSLHSQFPGR VHFFLSKFSR LREALQHFDH NQVDGVVFDV GVSSMQLSDP DRGFSFMKEG PLDMRMDMLR SQSKSAATFV NSLSEKDMAD VIFYYGGERL SRRVTRAIIS ARESNNKIRN TSDLAKIIRS VVPRSKANPI DPATRTFQAI RIWVNDELEE LKAGLEAASQ IVGVGGKIIV TSFHSLEDRI VKHKFNALRE KGFQLINKKA IKPTEKEISE NTRSRSAKLR GIVKEE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggattctcat agacctgtac tactaaacga aatgctagag cttctttctc ctaaagacgg tgcagtatat gttgacgcaa cgtttggtgg aggcggctat agtaggggaa tattggaacg ggctaaatgt actgtactag ctattgatca agatcctatt gcttcagaat tctatcaatc tttacattcg caattccctg gacgggtgca cttttttctc agcaagttca gtaggcttcg agaggcgtta cagcattttg atcacaatca agtagatggt gttgtatttg atgttggagt atcaagtatg cagctgagtg atccagacag gggattctca tttatgaagg aagggccact tgatatgaga atggatatgt tgcgctctca atccaaaagc gccgcgactt ttgtaaattc tttatcagaa aaagacatgg cagatgtaat cttctattac ggaggagaga ggctttcaag acgcgttacc agagctataa tcagcgctag agagagtaat aataagatac gtaacacgag tgacttggca aaaattatca ggtctgttgt gccccgcagt aaggcaaatc caatagatcc ggcaacaagg acctttcagg ctatcagaat atgggtaaat gatgagctag aagagctaaa agcaggtcta gaagctgctt ctcaaattgt aggagttgga ggcaagatta ttgttacttc atttcattca ctcgaggata ggatagtaaa acataagttc aacgctctac gagagaaggg atttcaactt atcaataaaa aagctataaa gcccactgaa aaagaaatta gcgaaaatac taggtcgcga tcagcaaaat tgcgtggcat agtaaaagag gagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa