AnphA.01070.a

fructose-bisphosphate aldolase

CENTER ID: AnphA.01070.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.01070.a.A1.GE27017 Full length( AnphA.01070.a ) 1 306
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1350
RefSeq: YP_505779.1
UniProt: Q2GIM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MICSGIMGLS EPVESVLDFY DSENPGVKTN LVRMLTHGAL AGTGRLLILP VDQGFEHGPV RSFGVNPSAC DPHYHYRLAR DCGFSAYAAP LGMLECGAAM YAGQIPTILK LNGSTALTPK STPPTQVITA SVKDALRLGC VAIGLTIYPG SESFFSMVRE VKELIHEAKM CGLAVVVWSY PRGGDISKEG ETSLDIISYA AHVAASLGAH IIKVKLPTAR IERDNVEPDM LSSLAQRVAY VKRCCFAGRR MVVFSGGDTK DDESLFQEVA AVRAGGGDGS IIGRNTFQRS EKDAVSLISS IVKLYS
NT Sequence
ATGATTTGCA GTGGCATTAT GGGCTTGAGT GAACCGGTTG AGAGTGTTTT GGATTTTTAC GACAGTGAGA ACCCAGGTGT TAAAACTAAC CTTGTGAGGA TGCTGACGCA TGGCGCTCTT GCTGGTACGG GTAGGCTGCT TATTTTACCG GTAGATCAAG GGTTTGAGCA TGGTCCTGTG CGCAGTTTTG GCGTAAATCC TAGTGCTTGC GATCCGCATT ACCATTATAG GCTTGCGAGG GATTGTGGAT TTAGTGCCTA TGCGGCGCCT TTGGGTATGT TGGAGTGTGG CGCGGCGATG TATGCAGGGC AGATTCCAAC CATACTGAAG CTCAATGGTT CTACGGCGTT AACTCCTAAA AGTACTCCCC CTACGCAGGT GATTACGGCT TCGGTTAAGG ATGCCCTGCG TCTTGGATGT GTTGCTATAG GCTTGACCAT ATATCCAGGG TCAGAATCGT TTTTCTCGAT GGTAAGGGAG GTTAAAGAGC TAATACATGA GGCGAAGATG TGTGGGTTGG CTGTTGTTGT ATGGTCATAC CCGCGCGGTG GCGATATATC TAAGGAAGGC GAGACTTCAT TGGATATTAT ATCGTATGCA GCGCACGTTG CTGCCTCACT TGGAGCGCAC ATTATCAAGG TGAAGTTACC TACTGCACGT ATAGAACGTG ATAATGTTGA ACCTGATATG CTATCTTCTT TGGCGCAAAG GGTGGCATAT GTTAAGAGGT GTTGCTTCGC GGGGCGAAGA ATGGTAGTGT TCTCTGGTGG GGATACTAAG GACGATGAAA GTTTGTTCCA AGAAGTAGCT GCTGTGAGGG CAGGTGGAGG TGATGGGTCT ATTATAGGCC GCAACACGTT CCAGAGATCC GAGAAGGATG CTGTCTCTCT AATTTCTAGT ATAGTAAAGC TATATAGC
Details for AnphA.01070.a.A1.GE27017
HARVESTED ON: 7/6/2009
SEQUENCED ON: 7/21/2009
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMICSGIMGL SEPVESVLDF YDSENPGVKT NLVRMLTHGA LAGTGRLLIL PVDQGFEHGP VRSFGVNPSA CDPHYHYRLA RDCGFSAYAA PLGMLECGAA MYAGQIPTIL KLNGSTALTP KSTPPTQVIT ASVKDALRLG CVAIGLTIYP GSESFFSMVR EVKELIHEAK MCGLAVVVWS YPRGGDISKE GETSLDIISY AAHVAASLGA HIIKVKLPTA RIERDNVEPD MLSSLAQRVA YVKRCCFAGR RMVVFSGGDT KDDESLFQEV AAVRAGGGDG SIIGRNTFQR SEKDAVSLIS SIVKL
Validated NT Sequence
atagctttac tatactagaa attagagaga cagcatcctt ctcggatctc tggaacgtgt tgcggcctat aatagaccca tcacctccac ctgccctcac agcagctact tcttggaaca aactttcatc gtccttagta tccccaccag agaacactac cattcttcgc cccgcgaagc aacacctctt aacatatgcc accctttgcg ccaaagaaga tagcatatca ggttcaacat tatcacgttc tatacgtgca gtaggtaact tcaccttgat aatgtgcgct ccaagtgagg cagcaacgtg cgctgcatac gatataatat ccaatgaagt ctcgccttcc ttagatatat cgccaccgcg cgggtatgac catacaacaa cagccaaccc acacatcttc gcctcatgta ttagctcttt aacctccctt accatcgaga aaaacgattc tgaccctgga tatatggtca agcctatagc aacacatcca agacgcaggg catccttaac cgaagccgta atcacctgcg tagggggagt acttttagga gttaacgccg tagaaccatt gagcttcagt atggttggaa tctgccctgc atacatcgcc gcgccacact ccaacatacc caaaggcgcc gcataggcac taaatccaca atccctcgca agcctataat ggtaatgcgg atcgcaagca ctaggattta cgccaaaact gcgcacagga ccatgctcaa acccttgatc taccggtaaa ataagcagcc tacccgtacc agcaagagcg ccatgcgtca gcatcctcac aaggttagtt ttaacacctg ggttctcact gtcgtaaaaa tccaaaacac tctcaaccgg ttcactcaag cccataatgc cactgcaaat catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tanctccttn tta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMICSGIMGL SEPVESVLDF YDSENPGVKT NLVRMLTHGA LAGTGRLLIL PVDQGFEHGP VRSFGVNPSA CDPHYHYRLA RDCGFSAYAA PLGMLECGAA MYAGQIPTIL KLNGSTALTP KSTPPTQVIT ASVKDALRLG CVAIGLTIYP GSESFFSMVR EVKELIHEAK MCGLAVVVWS YPRGGDISKE GETSLDIISY AAHVAASLGA HIIKVKLPTA RIERDNVEPD MLSSLAQRVA YVKRCCFAGR RMVVFSGGDT KDDESLFQEV AAVRAGGGDG SIIGRNTFQR SEKDAVSLIS SIVKLYS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatttgcagt ggcattatgg gcttgagtga accggttgag agtgttttgg atttttacga cagtgagaac ccaggtgtta aaactaacct tgtgaggatg ctgacgcatg gcgctcttgc tggtacgggt aggctgctta ttttaccggt agatcaaggg tttgagcatg gtcctgtgcg cagttttggc gtaaatccta gtgcttgcga tccgcattac cattataggc ttgcgaggga ttgtggattt agtgcctatg cggcgccttt gggtatgttg gagtgtggcg cggcgatgta tgcagggcag attccaacca tactgaagct caatggttct acggcgttaa ctcctaaaag tactccccct acgcaggtga ttacggcttc ggttaaggat gccctgcgtc ttggatgtgt tgctataggc ttgaccatat atccagggtc agaatcgttt ttctcgatgg taagggaggt taaagagcta atacatgagg cgaagatgtg tgggttggct gttgttgtat ggtcataccc gcgcggtggc gatatatcta aggaaggcga gacttcattg gatattatat cgtatgcagc gcacgttgct gcctcacttg gagcgcacat tatcaaggtg aagttaccta ctgcacgtat agaacgtgat aatgttgaac ctgatatgct atcttctttg gcgcaaaggg tggcatatgt taagaggtgt tgcttcgcgg ggcgaagaat ggtagtgttc tctggtgggg atactaagga cgatgaaagt ttgttccaag aagtagctgc tgtgagggca ggtggaggtg atgggtctat tataggccgc aacacgttcc agagatccga gaaggatgct gtctctctaa tttctagtat agtaaagcta tatagcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa