AnphA.01028.a

tyrosyl-tRNA synthetase

CENTER ID:	AnphA.01028.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01028.a.A1.GE27013	Full length( AnphA.01028.a )		1	412

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.01028.a.A1.PW27053	Full length( AnphA.01028.a )		1	412

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127413
PATRIC ID:	fig\|212042.8.peg.1336
RefSeq:	YP_505770.1
UniProt:	Q2GIN1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEFNSEFLAT LCARGYLHQL TDATALDGIM SSGAVTAYIG FDCTANSLHI GSLMQIMLLR YLQRFGHKPI VLLGGATTKV GDPSGKEKTR AMLSEDNISA NKEGILRVIK KFLSEDVVVV DNAEWLGNYG YLDFLREIGS KFSVNVMLGL ESVKSRLSRD QQLSFLEFSY VLLQSYDFVE LHKRYGCILQ IGGSDQWGNI VSGIDLARKM GCPQLYGVTT PLLLNSSGAK MGKTADGAIW LDETLYSPYN YWQYFRNVPD QDVGRLLRLF TELPLSEIER LEALKGEELN EAKKILATAV TSICHGAEVA LQVENQALKV FEHNDHDELR SITFSRKTLG QGIPISKLLH MWGLEESISA GRRLIRGGGC KINGGVVLDE EKLLNCGDFD DNGGYVAVFC GKKRRLKVVL ED

NT Sequence

ATGGAATTTA ATTCAGAATT TCTGGCTACT CTTTGTGCGC GGGGTTACCT GCACCAACTC ACTGATGCTA CAGCTTTAGA CGGTATAATG AGCTCCGGGG CAGTAACCGC GTATATTGGC TTTGATTGTA CGGCTAATAG CCTACATATT GGAAGCTTGA TGCAGATTAT GCTTCTCAGG TATCTTCAAA GATTCGGACA CAAGCCCATC GTGCTTTTGG GAGGTGCCAC TACCAAGGTA GGTGATCCTT CCGGGAAGGA GAAAACAAGA GCCATGCTCT CCGAAGATAA CATATCTGCA AATAAAGAGG GCATACTAAG AGTAATTAAA AAATTTCTGT CAGAGGACGT TGTAGTTGTA GATAACGCCG AATGGCTTGG TAACTATGGT TATCTGGATT TTTTAAGGGA AATTGGTAGT AAATTCTCAG TAAACGTGAT GCTAGGGCTT GAGAGCGTAA AGAGTCGCCT CAGCAGGGAT CAACAATTAA GTTTTTTGGA ATTTAGCTAC GTGCTGCTTC AGTCATACGA TTTTGTGGAA CTACACAAAA GATATGGTTG TATTTTGCAG ATAGGTGGGT CCGATCAATG GGGTAATATC GTCAGTGGTA TTGATCTGGC AAGAAAGATG GGGTGTCCAC AGCTCTACGG AGTGACCACT CCACTGCTGC TTAATAGTTC TGGAGCTAAA ATGGGGAAGA CAGCCGATGG CGCCATCTGG TTGGATGAAA CTTTGTATAG TCCCTATAAT TATTGGCAGT ATTTTCGTAA TGTTCCCGAT CAAGACGTAG GCCGATTGCT TAGATTGTTT ACGGAACTGC CTCTTAGTGA AATAGAGAGG TTGGAAGCCT TGAAAGGAGA GGAACTGAAT GAGGCAAAAA AGATATTGGC CACAGCGGTA ACTAGTATAT GCCACGGTGC TGAGGTGGCT CTGCAAGTTG AGAATCAAGC ACTAAAAGTT TTCGAACACA ACGATCATGA TGAACTGCGC AGTATTACCT TTAGTAGAAA GACTCTAGGC CAGGGAATCC CTATTTCCAA GTTGCTCCAT ATGTGGGGGC TTGAGGAGAG TATTAGTGCT GGGCGAAGGC TCATCAGGGG AGGTGGATGT AAAATTAATG GTGGTGTTGT CTTAGATGAA GAGAAGCTCT TGAATTGCGG AGATTTTGAC GATAACGGTG GATATGTTGC TGTATTTTGT GGTAAAAAAC GTCGTCTTAA GGTAGTATTA GAAGAC

Details for AnphA.01028.a.A1.GE27013

HARVESTED ON:	7/6/2009
SEQUENCED ON:	7/21/2009
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MEFNSXFLAT LCARGYLHQL TDATALDGIM SSGAVTAYIG FDCTANSLHI GSLMQIMLLR YLQRFGHKPI VLLGGATTKV GDPSGKEKTR AMLSEDNISA NKEGILRVIK KFLSEDVVVV DNAEWLGNYG YLDFLREIGS KFSVNVMLGL ESVKSRLSRD QQLSFLEFSY VLLQSYDFVE LHKRYGCILQ IGGSDQWGNI VSGIDLARKM GCPQLYGVTT PLLLNSSGAK MGKTADGAIW LDETLYSPYN YWQYFRNVPD QDVGRLLRLF TELPLSEIER LEALKGEELN EAKKILATAV TSICHGAEVA LQVENQALKV FEHNDHDELR SITFSRKTLG QGIPISKLLH MWGLEESISA GRRLIRGGGC KINGGVVLDE EKLLNCGDFD DNGGYVAVFC GKKRRLKVVL

Validated NT Sequence

taccctggaa gctcagaccc agggtcctgg ttcgatggaa tttaattcan aatttctggc tactctttgt gcgcggggtt acctgcacca actcactgat gctacagctt tagacggtat aatgagctcc ggggcagtaa ccgcgtatat tggctttgat tgtacggcta atagcctaca tattggaagc ttgatgcaga ttatgcttct caggtatctt caaagattcg gacacaagcc catcgtgctt ttgggaggtg ccactaccaa ggtaggtgat ccttccggga aggagaaaac aagagccatg ctctccgaag ataacatatc tgcaaataaa gagggcatac taagagtaat taaaaaattt ctgtcagagg acgttgtagt tgtagataac gccgaatggc ttggtaacta tggttatctg gattttttaa gggaaattgg tagtaaattc tcagtaaacg tgatgctagg gcttgagagc gtaaagagtc gcctcagcag ggatcaacaa ttaagttttt tggaatttag ctacgtgctg cttcagtcat acgattttgt ggaactacac aaaagatatg gttgtatttt gcagataggt gggtccgatc aatggggtaa tatcgtcagt ggtattgatc tggcaagaaa gatggggtgt ccacagctct acggagtgac cactccactg ctgcttaata gttctggagc taaaatgggg aagacagccg atggcgccat ctggttggat gaaactttgt atagtcccta taattattgg cagtattttc gtaatgttcc cgatcaagac gtaggccgat tgcttagatt gtttacggaa ctgcctctta gtgaaataga gaggttggaa gccttgaaag gagaggaact gaatgaggca aaaaagatat tggccacggc ggtaactagt atatgccacg gtgctgaggt ggctctgcaa gttgagaatc aagcactaaa agttttcgaa cacaacgatc atgatgaact gcgcagtatt acctttagta gaaagactct aggccaggga atccctattt ccaagttgct ccatatgtgg gggcttgagg agagtattag tgctgggcga aggctcatca ggggaggtgg atgtaaaatt aatggtggtg ttgtcttaga tgaagagaag ctcttgaatt gcggagattt tgacgataac ggtggatatg ttgctgtatt ttgtggtaaa aaacgtcgtc ttaaggtagt attag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEFNSEFLA TLCARGYLHQ LTDATALDGI MSSGAVTAYI GFDCTANSLH IGSLMQIMLL RYLQRFGHKP IVLLGGATTK VGDPSGKEKT RAMLSEDNIS ANKEGILRVI KKFLSEDVVV VDNAEWLGNY GYLDFLREIG SKFSVNVMLG LESVKSRLSR DQQLSFLEFS YVLLQSYDFV ELHKRYGCIL QIGGSDQWGN IVSGIDLARK MGCPQLYGVT TPLLLNSSGA KMGKTADGAI WLDETLYSPY NYWQYFRNVP DQDVGRLLRL FTELPLSEIE RLEALKGEEL NEAKKILATA VTSICHGAEV ALQVENQALK VFEHNDHDEL RSITFSRKTL GQGIPISKLL HMWGLEESIS AGRRLIRGGG CKINGGVVLD EEKLLNCGDF DDNGGYVAVF CGKKRRLKVV LED

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaatttaat tcagaatttc tggctactct ttgtgcgcgg ggttacctgc accaactcac tgatgctaca gctttagacg gtataatgag ctccggggca gtaaccgcgt atattggctt tgattgtacg gctaatagcc tacatattgg aagcttgatg cagattatgc ttctcaggta tcttcaaaga ttcggacaca agcccatcgt gcttttggga ggtgccacta ccaaggtagg tgatccttcc gggaaggaga aaacaagagc catgctctcc gaagataaca tatctgcaaa taaagagggc atactaagag taattaaaaa atttctgtca gaggacgttg tagttgtaga taacgccgaa tggcttggta actatggtta tctggatttt ttaagggaaa ttggtagtaa attctcagta aacgtgatgc tagggcttga gagcgtaaag agtcgcctca gcagggatca acaattaagt tttttggaat ttagctacgt gctgcttcag tcatacgatt ttgtggaact acacaaaaga tatggttgta ttttgcagat aggtgggtcc gatcaatggg gtaatatcgt cagtggtatt gatctggcaa gaaagatggg gtgtccacag ctctacggag tgaccactcc actgctgctt aatagttctg gagctaaaat ggggaagaca gccgatggcg ccatctggtt ggatgaaact ttgtatagtc cctataatta ttggcagtat tttcgtaatg ttcccgatca agacgtaggc cgattgctta gattgtttac ggaactgcct cttagtgaaa tagagaggtt ggaagccttg aaaggagagg aactgaatga ggcaaaaaag atattggcca cagcggtaac tagtatatgc cacggtgctg aggtggctct gcaagttgag aatcaagcac taaaagtttt cgaacacaac gatcatgatg aactgcgcag tattaccttt agtagaaaga ctctaggcca gggaatccct atttccaagt tgctccatat gtgggggctt gaggagagta ttagtgctgg gcgaaggctc atcaggggag gtggatgtaa aattaatggt ggtgttgtct tagatgaaga gaagctcttg aattgcggag attttgacga taacggtgga tatgttgctg tattttgtgg taaaaaacgt cgtcttaagg tagtattaga agacaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for AnphA.01028.a.A1.PW27053

PURIFICATION DATe:	7/28/2009
CONCENTRATION:	28.18mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAATTTAAT TCAGAATTTC TGGCTACTCT TTGTGCGCGG GGTTACCTGC ACCAACTCAC TGATGCTACA GCTTTAGACG GTATAATGAG CTCCGGGGCA GTAACCGCGT ATATTGGCTT TGATTGTACG GCTAATAGCC TACATATTGG AAGCTTGATG CAGATTATGC TTCTCAGGTA TCTTCAAAGA TTCGGACACA AGCCCATCGT GCTTTTGGGA GGTGCCACTA CCAAGGTAGG TGATCCTTCC GGGAAGGAGA AAACAAGAGC CATGCTCTCC GAAGATAACA TATCTGCAAA TAAAGAGGGC ATACTAAGAG TAATTAAAAA ATTTCTGTCA GAGGACGTTG TAGTTGTAGA TAACGCCGAA TGGCTTGGTA ACTATGGTTA TCTGGATTTT TTAAGGGAAA TTGGTAGTAA ATTCTCAGTA AACGTGATGC TAGGGCTTGA GAGCGTAAAG AGTCGCCTCA GCAGGGATCA ACAATTAAGT TTTTTGGAAT TTAGCTACGT GCTGCTTCAG TCATACGATT TTGTGGAACT ACACAAAAGA TATGGTTGTA TTTTGCAGAT AGGTGGGTCC GATCAATGGG GTAATATCGT CAGTGGTATT GATCTGGCAA GAAAGATGGG GTGTCCACAG CTCTACGGAG TGACCACTCC ACTGCTGCTT AATAGTTCTG GAGCTAAAAT GGGGAAGACA GCCGATGGCG CCATCTGGTT GGATGAAACT TTGTATAGTC CCTATAATTA TTGGCAGTAT TTTCGTAATG TTCCCGATCA AGACGTAGGC CGATTGCTTA GATTGTTTAC GGAACTGCCT CTTAGTGAAA TAGAGAGGTT GGAAGCCTTG AAAGGAGAGG AACTGAATGA GGCAAAAAAG ATATTGGCCA CAGCGGTAAC TAGTATATGC CACGGTGCTG AGGTGGCTCT GCAAGTTGAG AATCAAGCAC TAAAAGTTTT CGAACACAAC GATCATGATG AACTGCGCAG TATTACCTTT AGTAGAAAGA CTCTAGGCCA GGGAATCCCT ATTTCCAAGT TGCTCCATAT GTGGGGGCTT GAGGAGAGTA TTAGTGCTGG GCGAAGGCTC ATCAGGGGAG GTGGATGTAA AATTAATGGT GGTGTTGTCT TAGATGAAGA GAAGCTCTTG AATTGCGGAG ATTTTGACGA TAACGGTGGA TATGTTGCTG TATTTTGTGG TAAAAAACGT CGTCTTAAGG TAGTATTAGA AGACAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA