AnphA.01019.a
dihydropteroate synthase
| CENTER ID: | AnphA.01019.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01019.a.A1.GE27012 | Full length( AnphA.01019.a ) | 1 | 270 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.01019.a.A1.PW27052 | Full length( AnphA.01019.a ) | 1 | 270 |
Structures
6UCZ
DEPOSITED: 9/18/2019
DETERMINATION: XRay
CLONE: AnphA.01019.a.A1.GE27012
PROTEIN: AnphA.01019.a.A1.PW27052
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128803 |
| PATRIC ID: | fig|212042.8.peg.265 |
| RefSeq: | YP_504869.1 |
| UniProt: | Q2GL82 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQHSAVTTKI VGILNVTPNS FSDGGKFLEA NAAIRHATDL IEHGADVIDV GAESTAPGVS PITQEEEWSR LKEVLPEIIR IAHNAHVEVS IDTRNAKTAE LALKLGVDYI NDQGGLLDPD MPAIAAASSA KIIIMHHFGL PVSQDRSYVG QPEQLLNEII EWLCSRVDTL IAQGVVRERI ILDPGLGFGK LPEYSWYIAK NIGKLKKLGF PVCVGHSRKS MFSLIPVELS ERDVPTAMLS TFLAQQQVDF LRVHDVKLTG ISIKIANLLV
NT Sequence
TTGCAACACT CTGCTGTCAC CACAAAAATT GTTGGTATAT TGAATGTAAC CCCAAATTCG TTCTCCGACG GGGGTAAGTT TTTGGAAGCT AATGCTGCAA TTAGACACGC TACAGATCTA ATAGAGCATG GTGCAGATGT GATTGATGTA GGTGCGGAGT CCACAGCACC TGGCGTATCT CCCATAACAC AGGAAGAAGA ATGGAGCAGG CTGAAGGAGG TTTTACCTGA AATCATACGC ATTGCTCATA ATGCACATGT AGAAGTCAGC ATAGATACAA GAAATGCTAA GACCGCTGAG CTAGCTCTGA AACTGGGTGT AGATTACATA AATGATCAGG GAGGTCTTCT GGATCCTGAT ATGCCGGCAA TCGCGGCAGC AAGTTCTGCC AAAATCATTA TTATGCACCA CTTTGGGCTG CCCGTATCCC AAGACAGAAG CTATGTTGGG CAACCTGAAC AGTTACTAAA TGAAATAATA GAATGGCTGT GCTCGAGAGT TGATACTCTC ATTGCGCAGG GTGTAGTTCG TGAACGCATA ATTTTAGATC CGGGTCTCGG CTTTGGTAAG TTGCCTGAAT ACTCGTGGTA CATTGCTAAA AACATAGGGA AACTAAAAAA ACTTGGCTTT CCAGTCTGCG TGGGGCATTC ACGCAAATCT ATGTTTTCTT TGATACCCGT TGAACTATCA GAAAGAGATG TTCCAACGGC CATGCTGTCC ACGTTTCTTG CACAACAACA GGTAGACTTC CTGCGTGTCC ATGACGTGAA ATTAACTGGA ATATCTATCA AAATAGCAAA CTTGCTTGTG
Details for AnphA.01019.a.A1.GE27012
| HARVESTED ON: | 7/6/2009 |
| SEQUENCED ON: | 7/21/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLQHSAVTT KIVGILNVTP NSFSDGGKFL EANAAIRHAT DLIEHGADVI DVGAESTAPG VSPITQEEEW SRLKEVLPEI IRIAHNAHVE VSIDTRNAKT AELALKLGVD YINDQGGLLD PDMPAIAAAS SAKIIIMHHF GLPVSQDRSY VGQPEQLLNE IIEWLCSRVD TLIAQGVVRE RIILDPGLGF GKLPEYSWYI AKNIGKLKKL GFPVCVGHSR KSMFSLIPVE LSERDVPTAM LSTFLAQQQV DFLRVHDVKL TGISIKIANL LV
Validated NT Sequence
ttattacaca agcaagtttg ctattttgat agatatttca gttgattcca cgtagcggcc acgcatggag tctacctgtt gttctgcaag aagcatagat agcaacgctt atggaacgac aactcaacag gtattaatga agagaactgc cgtaggtaga aattacacaa gcaagtttgc tattttgata gatattccag ttaatttcac gtcatggaca cgcaggaagt ctacctgttg ttgtgcaaga aacgtggaca gcatggccgt tggaacatct ctttctgata gttcaacggg tatcaaagaa aacatagatt tgcgtgaatg ccccacgcag actggaaagc caagtttttt tagtttccct atgtttttag caatgtacca cgagtattca ggcaacttac caaagccgag acccggatct aaaattatgc gttcacgaac tacaccctgc gcaatgagag tatcaactct cgagcacagc cattctatta tttcatttag taactgttca ggttgcccaa catagcttct gtcttgggat acgggcagcc caaagtggtg cataataatg attttggcag aacttgctgc cgcgattgcc ggcatatcag gatccagaag acctccctga tcatttatgt aatctacacc cagtttcaga gctagctcag cggtcttagc atttcttgta tctatgctga cttctacatg tgcattatga gcaatgcgta tgatttcagg taaaacctcc ttcagcctgc tccattcttc ttcctgtgtt atgggagata cgccaggtgc tgtggactcc gcacctacat caatcacatc tgcaccatgc tctattagat ctgtagcgtg tctaattgca gcattagctt ccaaaaactt acccccgtcg gagaacgaat ttggggttac attcaatata ccaacaattt ttgtggtgac agcagagtgt tgcaacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtatnnct ccttcttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLQHSAVTT KIVGILNVTP NSFSDGGKFL EANAAIRHAT DLIEHGADVI DVGAESTAPG VSPITQEEEW SRLKEVLPEI IRIAHNAHVE VSIDTRNAKT AELALKLGVD YINDQGGLLD PDMPAIAAAS SAKIIIMHHF GLPVSQDRSY VGQPEQLLNE IIEWLCSRVD TLIAQGVVRE RIILDPGLGF GKLPEYSWYI AKNIGKLKKL GFPVCVGHSR KSMFSLIPVE LSERDVPTAM LSTFLAQQQV DFLRVHDVKL TGISIKIANL LV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcaacac tctgctgtca ccacaaaaat tgttggtata ttgaatgtaa ccccaaattc gttctccgac gggggtaagt ttttggaagc taatgctgca attagacacg ctacagatct aatagagcat ggtgcagatg tgattgatgt aggtgcggag tccacagcac ctggcgtatc tcccataaca caggaagaag aatggagcag gctgaaggag gttttacctg aaatcatacg cattgctcat aatgcacatg tagaagtcag catagataca agaaatgcta agaccgctga gctagctctg aaactgggtg tagattacat aaatgatcag ggaggtcttc tggatcctga tatgccggca atcgcggcag caagttctgc caaaatcatt attatgcacc actttgggct gcccgtatcc caagacagaa gctatgttgg gcaacctgaa cagttactaa atgaaataat agaatggctg tgctcgagag ttgatactct cattgcgcag ggtgtagttc gtgaacgcat aattttagat ccgggtctcg gctttggtaa gttgcctgaa tactcgtggt acattgctaa aaacataggg aaactaaaaa aacttggctt tccagtctgc gtggggcatt cacgcaaatc tatgttttct ttgatacccg ttgaactatc agaaagagat gttccaacgg ccatgctgtc cacgtttctt gcacaacaac aggtagactt cctgcgtgtc catgacgtga aattaactgg aatatctatc aaaatagcaa acttgcttgt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.01019.a.A1.PW27052
| PURIFICATION DATe: | 7/21/2009 |
| CONCENTRATION: | 26.99mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLQHSAVTT KIVGILNVTP NSFSDGGKFL EANAAIRHAT DLIEHGADVI DVGAESTAPG VSPITQEEEW SRLKEVLPEI IRIAHNAHVE VSIDTRNAKT AELALKLGVD YINDQGGLLD PDMPAIAAAS SAKIIIMHHF GLPVSQDRSY VGQPEQLLNE IIEWLCSRVD TLIAQGVVRE RIILDPGLGF GKLPEYSWYI AKNIGKLKKL GFPVCVGHSR KSMFSLIPVE LSERDVPTAM LSTFLAQQQV DFLRVHDVKL TGISIKIANL LV
Validated NT Sequence
ttattacaca agcaagtttg ctattttgat agatatttca gttgattcca cgtagcggcc acgcatggag tctacctgtt gttctgcaag aagcatagat agcaacgctt atggaacgac aactcaacag gtattaatga agagaactgc cgtaggtaga aattacacaa gcaagtttgc tattttgata gatattccag ttaatttcac gtcatggaca cgcaggaagt ctacctgttg ttgtgcaaga aacgtggaca gcatggccgt tggaacatct ctttctgata gttcaacggg tatcaaagaa aacatagatt tgcgtgaatg ccccacgcag actggaaagc caagtttttt tagtttccct atgtttttag caatgtacca cgagtattca ggcaacttac caaagccgag acccggatct aaaattatgc gttcacgaac tacaccctgc gcaatgagag tatcaactct cgagcacagc cattctatta tttcatttag taactgttca ggttgcccaa catagcttct gtcttgggat acgggcagcc caaagtggtg cataataatg attttggcag aacttgctgc cgcgattgcc ggcatatcag gatccagaag acctccctga tcatttatgt aatctacacc cagtttcaga gctagctcag cggtcttagc atttcttgta tctatgctga cttctacatg tgcattatga gcaatgcgta tgatttcagg taaaacctcc ttcagcctgc tccattcttc ttcctgtgtt atgggagata cgccaggtgc tgtggactcc gcacctacat caatcacatc tgcaccatgc tctattagat ctgtagcgtg tctaattgca gcattagctt ccaaaaactt acccccgtcg gagaacgaat ttggggttac attcaatata ccaacaattt ttgtggtgac agcagagtgt tgcaacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtatnnct ccttcttaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLQHSAVTT KIVGILNVTP NSFSDGGKFL EANAAIRHAT DLIEHGADVI DVGAESTAPG VSPITQEEEW SRLKEVLPEI IRIAHNAHVE VSIDTRNAKT AELALKLGVD YINDQGGLLD PDMPAIAAAS SAKIIIMHHF GLPVSQDRSY VGQPEQLLNE IIEWLCSRVD TLIAQGVVRE RIILDPGLGF GKLPEYSWYI AKNIGKLKKL GFPVCVGHSR KSMFSLIPVE LSERDVPTAM LSTFLAQQQV DFLRVHDVKL TGISIKIANL LV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGCAACAC TCTGCTGTCA CCACAAAAAT TGTTGGTATA TTGAATGTAA CCCCAAATTC GTTCTCCGAC GGGGGTAAGT TTTTGGAAGC TAATGCTGCA ATTAGACACG CTACAGATCT AATAGAGCAT GGTGCAGATG TGATTGATGT AGGTGCGGAG TCCACAGCAC CTGGCGTATC TCCCATAACA CAGGAAGAAG AATGGAGCAG GCTGAAGGAG GTTTTACCTG AAATCATACG CATTGCTCAT AATGCACATG TAGAAGTCAG CATAGATACA AGAAATGCTA AGACCGCTGA GCTAGCTCTG AAACTGGGTG TAGATTACAT AAATGATCAG GGAGGTCTTC TGGATCCTGA TATGCCGGCA ATCGCGGCAG CAAGTTCTGC CAAAATCATT ATTATGCACC ACTTTGGGCT GCCCGTATCC CAAGACAGAA GCTATGTTGG GCAACCTGAA CAGTTACTAA ATGAAATAAT AGAATGGCTG TGCTCGAGAG TTGATACTCT CATTGCGCAG GGTGTAGTTC GTGAACGCAT AATTTTAGAT CCGGGTCTCG GCTTTGGTAA GTTGCCTGAA TACTCGTGGT ACATTGCTAA AAACATAGGG AAACTAAAAA AACTTGGCTT TCCAGTCTGC GTGGGGCATT CACGCAAATC TATGTTTTCT TTGATACCCG TTGAACTATC AGAAAGAGAT GTTCCAACGG CCATGCTGTC CACGTTTCTT GCACAACAAC AGGTAGACTT CCTGCGTGTC CATGACGTGA AATTAACTGG AATATCTATC AAAATAGCAA ACTTGCTTGT GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA