AnphA.00971.a

ABC transporter, ATP-binding protein

CENTER ID: AnphA.00971.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00971.a.MB1.GE30416 Full length( AnphA.00971.a ) 1 342
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1051
RefSeq: YP_505549.1
UniProt: Q2GJA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEGLELKDIS YRYRKSDFLL EVEELSAKRG EIICLLGPSG CGKSTILKLI AGLERPFAGV IRINGKTVND ARGGVYIPTE SRNVGLIFQH PALFQHQTIV ENVAFAVKAQ SRDKRTKHAL KVLKKLGISA HKDAYPHMLS GGQQQLATIA RTMAQDPEIV LLDEPFSNLD TILRESIRAA VLSVIKAENI TVLLVTHDPE EALEIADKIY VVREGKIVQC GTPYQIYNAP KDAHLARFFG RLNYFESLVR DGKVSLTIGS INADGFLDGS RVAVCIRPDA ILLHDSSDTL AVVKLVRFFN NMIYISVGNS AYWMLFLGAV LPEVGDVIRV SLDSSKALLF RL
NT Sequence
GTGGAAGGAC TTGAGCTTAA AGACATATCG TACAGGTATA GAAAGAGTGA TTTTCTTTTG GAAGTGGAAG AACTTTCTGC CAAAAGGGGC GAAATAATAT GTTTATTAGG GCCATCAGGG TGTGGAAAAT CCACAATATT AAAGTTAATA GCAGGACTAG AAAGGCCTTT TGCAGGGGTG ATAAGGATTA ATGGCAAAAC AGTAAATGAT GCCCGAGGTG GCGTTTATAT ACCAACAGAA TCCCGCAATG TAGGGCTAAT TTTCCAGCAC CCGGCACTGT TTCAACACCA AACTATAGTT GAGAATGTAG CATTCGCAGT AAAAGCGCAA AGCAGGGATA AGAGAACGAA GCATGCTTTG AAGGTATTGA AAAAGCTGGG AATTTCTGCT CACAAAGACG CCTATCCTCA TATGCTTTCG GGTGGTCAGC AACAACTTGC TACCATTGCG AGAACCATGG CTCAGGATCC TGAAATTGTC CTACTTGATG AGCCTTTTTC TAACCTTGAT ACCATCCTAA GGGAGTCGAT ACGCGCAGCT GTGTTATCTG TAATTAAGGC TGAGAATATC ACCGTGCTGT TAGTTACTCA TGATCCTGAA GAGGCTTTGG AGATAGCTGA CAAGATATAT GTGGTGCGAG AAGGCAAAAT AGTCCAGTGT GGTACTCCTT ACCAAATATA CAACGCACCC AAAGATGCTC ATCTTGCTCG GTTCTTTGGC AGGCTTAATT ATTTTGAGTC TCTAGTGCGT GATGGTAAGG TATCCCTCAC TATAGGCTCC ATAAATGCTG ACGGCTTCCT CGATGGTTCT AGGGTTGCTG TATGTATTAG GCCCGACGCC ATCCTCCTTC ATGACTCCAG CGATACTCTT GCTGTGGTAA AGCTTGTGAG ATTTTTTAAT AATATGATAT ACATATCTGT TGGCAATAGT GCTTACTGGA TGTTGTTTTT GGGTGCAGTT TTGCCTGAGG TAGGTGATGT AATACGTGTA TCTTTAGACT CGAGTAAGGC TTTGCTTTTT AGACTC
Details for AnphA.00971.a.MB1.GE30416
HARVESTED ON: 5/11/2010
SEQUENCED ON: 10/7/2010
EXPECTED MW: 82kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 86
PERCENT COVERAGE: 39
Validated AA Sequence
MVEGLELKDI SYRYRKSDFL LEVEELSAKR GEIICLLGPS GCGKSTILKL IAGLERPSAG VIRINGKTVN DARGGVYIPT ESRNVGLIFQ HPALFQHQTI VENVAFAVKA QSRDKRTKHA LKVLKKLGIS AHKDAYPHML SGGQQQLATI ARTMAQDPEI VLLDEPFSNL DTILRESIRA AVLSVIKXEN ITVLLVTHDP EEALEIADKI YVVREXKIVQ CGTPYQXYXA PXXAHLAXFF GXXNYXESLX RDGXVSLTXG SINAXXXXXX LXGXXMXXXX RXPPXMTXXI LLLXXSX
Validated NT Sequence
gnnctcagac ccagggtcct ggttcgatgg tggaaggact tgagcttaaa gacatatcat acaggtatag aaagagtgat tttcttttgg aagtggaaga actttctgcc aaaaggggcg aaataatatg tttattaggg ccatcagggt gtggaaaatc cacaatatta aagttaatag caggactaga aaggccttct gcaggggtga taaggattaa tggcaaaaca gtaaatgatg cccgaggtgg cgtttatata ccaacagaat cccgcaatgt agggctaatt ttccagcacc cggcactgtt tcaacaccaa actatagttg agaatgtagc attcgcagta aaagcgcaaa gcagggataa gagaacgaag catgctttga aggtattgaa aaagctggga atttctgctc acaaagacgc ctatcctcat atgctttcgg gtggtcagca acaacttgct accattgcga gaaccatggc tcaggatcct gaaattgtcc tacttgatga gcctttttct aaccttgata ccatcctaag ggagtcgata cgcgcagctg tgttatctgt aattaagnct gagaatatca ccgtgctgtt agttactcat gatcctgaag aggcnttgga gatagctgac aagatatatg tggtgcgaga agncaaaata gtccagtgtg gtactcctta ccaaanatac nacgcacccn nagangctca tctngctngg ttctttggcn ggcntaatta tnttgagtct ctagngcgtg atggnnaggt atccctcact anaggctcca taaatgctgn cngnnncnnc gnggntctnn ggggtngnng tatgnatnan gnnngacgcc ntcctccntn catgactnnn ncnatactct tgctgnngta nagctnntga naannnnttn atnnnatnnn nnncatancn nnnnncnnng nncnnnccnn nannntnnnt nnnnnngcan nnnttnnntn nngnnnnnnn nnnnnnnnnn ntnnnnnnan naannnnnnn nntnnnnnnc nnnnannnnn nnnnnnnnnn nnnnnnnnnn gnnannannn nnnnnnnnnn nnnnannnnn nnnnncnnnc
Expected Protein Sequence
MAHHHHHHMK IEEGKLVIWI NGDKGYNGLA EVGKKFEKDT GIKVTVEHPD KLEEKFPQVA ATGDGPDIIF WAHDRFGGYA QSGLLAEITP DKAFQDKLYP FTWDAVRYNG KLIAYPIAVE ALSLIYNKDL LPNPPKTWEE IPALDKELKA KGKSALMFNL QEPYFTWPLI AADGGYAFKY ENGKYDIKDV GVDNAGAKAG LTFLVDLIKN KHMNADTDYS IAEAAFNKGE TAMTINGPWA WSNIDTSKVN YGVTVLPTFK GQPSKPFVGV LSAGINAASP NKELAKEFLE NYLLTDEGLE AVNKDKPLGA VALKSYEEEL AKDPRIAATM ENAQKGEIMP NIPQMSAFWY AVRTAVINAA SGRQTVDEAL KDAQTNSSSN NNNNNNNNNL GIEGRISEGT LEAQTQGPGS MVEGLELKDI SYRYRKSDFL LEVEELSAKR GEIICLLGPS GCGKSTILKL IAGLERPFAG VIRINGKTVN DARGGVYIPT ESRNVGLIFQ HPALFQHQTI VENVAFAVKA QSRDKRTKHA LKVLKKLGIS AHKDAYPHML SGGQQQLATI ARTMAQDPEI VLLDEPFSNL DTILRESIRA AVLSVIKAEN ITVLLVTHDP EEALEIADKI YVVREGKIVQ CGTPYQIYNA PKDAHLARFF GRLNYFESLV RDGKVSLTIG SINADGFLDG SRVAVCIRPD AILLHDSSDT LAVVKLVRFF NNMIYISVGN SAYWMLFLGA VLPEVGDVIR VSLDSSKALL FRL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcaca tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg atttcagaag gtaccctgga agctcagacc cagggtcctg gttcgatggt ggaaggactt gagcttaaag acatatcgta caggtataga aagagtgatt ttcttttgga agtggaagaa ctttctgcca aaaggggcga aataatatgt ttattagggc catcagggtg tggaaaatcc acaatattaa agttaatagc aggactagaa aggccttttg caggggtgat aaggattaat ggcaaaacag taaatgatgc ccgaggtggc gtttatatac caacagaatc ccgcaatgta gggctaattt tccagcaccc ggcactgttt caacaccaaa ctatagttga gaatgtagca ttcgcagtaa aagcgcaaag cagggataag agaacgaagc atgctttgaa ggtattgaaa aagctgggaa tttctgctca caaagacgcc tatcctcata tgctttcggg tggtcagcaa caacttgcta ccattgcgag aaccatggct caggatcctg aaattgtcct acttgatgag cctttttcta accttgatac catcctaagg gagtcgatac gcgcagctgt gttatctgta attaaggctg agaatatcac cgtgctgtta gttactcatg atcctgaaga ggctttggag atagctgaca agatatatgt ggtgcgagaa ggcaaaatag tccagtgtgg tactccttac caaatataca acgcacccaa agatgctcat cttgctcggt tctttggcag gcttaattat tttgagtctc tagtgcgtga tggtaaggta tccctcacta taggctccat aaatgctgac ggcttcctcg atggttctag ggttgctgta tgtattaggc ccgacgccat cctccttcat gactccagcg atactcttgc tgtggtaaag cttgtgagat tttttaataa tatgatatac atatctgttg gcaatagtgc ttactggatg ttgtttttgg gtgcagtttt gcctgaggta ggtgatgtaa tacgtgtatc tttagactcg agtaaggctt tgctttttag actctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa