SSGCID: Target Page

AnphA.00936.b

Ferredoxin--NADP reductase

CENTER ID:	AnphA.00936.b
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00936.b.A1.PS01024	Full length( AnphA.00936.b )		1	338

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.787
RefSeq:	YP_505314.1
UniProt:	Q2GJY7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEYSSVFDVA IVGAGPVGLF TVFQAGMLGM NSCVIDALDI VGGQCAVLYP EKPIYDIPGY PMITAQDLVN NLKKQAEPFA PVYIMGQFVE SISEGPDCFV LQTNKGTSVK CRAIIVAAGS GGFGPNRPPL DGIMEYENKS VFYNVSQMAT FKNKKVVIAG GGDSAADWAV NLAEVADKLY VIHRRKSFRC APNTLKKLEE LADEGRINVL IPYQLAGLDG ENGELRSVVV RNITTKEEIP IGADFLLPFF GISANLGEIV NWGLGVEGFQ IPVEQSTCRT RRSKIYAVGD IAAYPGKIKL ILVGFSEAAL ACQDIRAVLF PDTPLNFQYS TSKGIPTL

NT Sequence

GTGGAGTATA GTTCTGTTTT CGATGTCGCT ATAGTAGGTG CAGGTCCTGT GGGACTTTTT ACCGTGTTTC AAGCAGGTAT GCTGGGAATG AATTCCTGTG TTATTGATGC TTTGGATATA GTAGGGGGTC AGTGCGCAGT TTTATATCCT GAAAAACCTA TTTATGATAT CCCAGGCTAT CCCATGATTA CGGCTCAAGA CCTGGTAAAT AACCTGAAAA AGCAGGCAGA ACCCTTTGCT CCCGTTTATA TCATGGGGCA ATTTGTCGAA AGCATAAGTG AAGGTCCTGA CTGCTTTGTT CTACAGACCA ATAAAGGTAC TAGCGTAAAG TGTCGAGCAA TAATAGTTGC AGCTGGGTCA GGAGGATTTG GCCCTAACCG CCCCCCACTT GACGGTATCA TGGAATACGA AAATAAGTCC GTATTCTATA ACGTATCTCA AATGGCCACC TTCAAGAACA AAAAGGTAGT AATAGCTGGT GGAGGAGATT CAGCTGCAGA CTGGGCTGTA AATTTGGCGG AAGTTGCTGA TAAGCTGTAT GTGATACATA GAAGAAAGTC TTTTCGTTGC GCACCTAATA CTTTAAAAAA ACTCGAAGAA TTAGCGGATG AAGGTCGCAT AAATGTGCTT ATTCCTTACC AACTTGCAGG GCTTGATGGT GAAAATGGAG AACTCAGAAG CGTTGTAGTC AGAAATATTA CCACGAAGGA GGAAATCCCT ATAGGAGCAG ATTTTCTCTT ACCATTCTTT GGAATATCTG CAAACCTGGG TGAGATTGTT AACTGGGGCT TGGGCGTAGA AGGCTTTCAG ATACCAGTAG AGCAATCTAC ATGTAGGACT AGACGTAGCA AAATATATGC CGTGGGTGAT ATAGCTGCTT ACCCCGGAAA GATAAAGCTT ATACTAGTAG GTTTTTCAGA GGCTGCTTTG GCATGTCAAG ATATACGTGC CGTGCTCTTT CCGGATACTC CTCTTAATTT TCAGTATTCT ACCTCAAAAG GTATTCCCAC GCTT

Details for AnphA.00936.b.A1.PS01024

PURIFICATION DATe:	3/31/2011
CONCENTRATION:	45.61mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	70

Protocol Notes

notes unavailable

Validated AA Sequence

MGQFVESISE XPDCFVLQTN KGTSVKCRAI IVAAGSGGFG PNRPXLDGIM EYENKSVFYN VSQMATFKNK KVVIAGGGDS AADWAVNLAE VADKLYVIHR RKSFRCAPNT LKKLEELADE GRINVLIPYQ LAGLDGENGE LRSVVVRNIT TKEEIPIGAD FLLPFFGISA NLGEIVNWGL GVEGFQIPVE QSTCRTRRSK IYAVGDIAAY PGKIKLILVG FSEAALACQD IRAVLFPDTP LNFQYSTSKG IPTL

Validated NT Sequence

tattaaagcg tgggaatacc ttttgaggta gaatactgaa aattaagagg agtatccgga aagagcacgg cacgtatatc ttgacatgcc aaagcagcct ctgaaaaacc tactagtata agctttatct ttccggggta agcagctata tcacccacgg catatatttt gctacgtcta gtcctacatg tagattgctc tactggtatc tgaaagcctt ctacgcccaa gccccagtta acaatctcac ccaggtttgc agatattcca aagaatggta agagaaaatc tgctcctata gggatttcct ccttcgtggt aatatttctg actacaacgc ttctgagttc tccattttca ccatcaagcc ctgcaagttg gtaaggaata agcacattta tgcgaccttc atccgctaat tcttcgagtt tttttaaagt attaggtgcg caacgaaaag actttcttct atgtatcaca tacagcttat cagcaacttc cgccaaattt acagcccagt ctgcagctga atctcctcca ccagctatta ctaccttttt gttcttgaag gtggccattt gagatacgtt atagaatacg gacttatttt cgtattccat gataccgtca agtgnggggc ggttagggcc aaatcctcct gacccagctg caactattat tgctcgacac tttacgctag tacctttatt ggtctgtaga acaaagcagt caggancttc acttatgctt tcgacaaatt gccccatgat ataaacggga gcaaagggtt ctgcctgctt tttc

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVEYSSVFD VAIVGAGPVG LFTVFQAGML GMNSCVIDAL DIVGGQCAVL YPEKPIYDIP GYPMITAQDL VNNLKKQAEP FAPVYIMGQF VESISEGPDC FVLQTNKGTS VKCRAIIVAA GSGGFGPNRP PLDGIMEYEN KSVFYNVSQM ATFKNKKVVI AGGGDSAADW AVNLAEVADK LYVIHRRKSF RCAPNTLKKL EELADEGRIN VLIPYQLAGL DGENGELRSV VVRNITTKEE IPIGADFLLP FFGISANLGE IVNWGLGVEG FQIPVEQSTC RTRRSKIYAV GDIAAYPGKI KLILVGFSEA ALACQDIRAV LFPDTPLNFQ YSTSKGIPTL

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGAGTAT AGTTCTGTTT TCGATGTCGC TATAGTAGGT GCAGGTCCTG TGGGACTTTT TACCGTGTTT CAAGCAGGTA TGCTGGGAAT GAATTCCTGT GTTATTGATG CTTTGGATAT AGTAGGGGGT CAGTGCGCAG TTTTATATCC TGAAAAACCT ATTTATGATA TCCCAGGCTA TCCCATGATT ACGGCTCAAG ACCTGGTAAA TAACCTGAAA AAGCAGGCAG AACCCTTTGC TCCCGTTTAT ATCATGGGGC AATTTGTCGA AAGCATAAGT GAAGGTCCTG ACTGCTTTGT TCTACAGACC AATAAAGGTA CTAGCGTAAA GTGTCGAGCA ATAATAGTTG CAGCTGGGTC AGGAGGATTT GGCCCTAACC GCCCCCCACT TGACGGTATC ATGGAATACG AAAATAAGTC CGTATTCTAT AACGTATCTC AAATGGCCAC CTTCAAGAAC AAAAAGGTAG TAATAGCTGG TGGAGGAGAT TCAGCTGCAG ACTGGGCTGT AAATTTGGCG GAAGTTGCTG ATAAGCTGTA TGTGATACAT AGAAGAAAGT CTTTTCGTTG CGCACCTAAT ACTTTAAAAA AACTCGAAGA ATTAGCGGAT GAAGGTCGCA TAAATGTGCT TATTCCTTAC CAACTTGCAG GGCTTGATGG TGAAAATGGA GAACTCAGAA GCGTTGTAGT CAGAAATATT ACCACGAAGG AGGAAATCCC TATAGGAGCA GATTTTCTCT TACCATTCTT TGGAATATCT GCAAACCTGG GTGAGATTGT TAACTGGGGC TTGGGCGTAG AAGGCTTTCA GATACCAGTA GAGCAATCTA CATGTAGGAC TAGACGTAGC AAAATATATG CCGTGGGTGA TATAGCTGCT TACCCCGGAA AGATAAAGCT TATACTAGTA GGTTTTTCAG AGGCTGCTTT GGCATGTCAA GATATACGTG CCGTGCTCTT TCCGGATACT CCTCTTAATT TTCAGTATTC TACCTCAAAA GGTATTCCCA CGCTTTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA