AnphA.00936.a

thioredoxin-disulfide reductase

CENTER ID: AnphA.00936.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00936.a.A1.GE27005 Full length( AnphA.00936.a ) 1 321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00936.a.A1.PW27045 Full length( AnphA.00936.a ) 1 321

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.708
RefSeq: YP_505240.1
UniProt: Q2GK61

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEVHKDAVK VLILGSGAAG CTAAIYAGRA NLNPVLVTGM QPGGQLTITT DVENYPGFSA IEGPKLMEYM QEQAVKSGAT LLTDEISEID ASVYPFKCTS MFGETLQAYS IIVATGAQAK WLGLESEQNF KSKGVSACAT CDGAFFKDEV VAVIGGGNTA VEEALYLTRS SKKVFLIHRR DKLRAEAVMQ QRLFSNSKIE VIWNSVVKEI LGDSEGKKVT SLLLQSTVDS TESLLDVGGV FVAIGHTPNT QVLKNMLGAK VNLDDEGYVI TAPGTTITSC PGIFAAGDVC DKVYRQAVVA AGSGCMAALD AEKYLAEKGI E
NT Sequence
ATGTCAGAAG TGCATAAAGA TGCAGTTAAA GTTCTGATTC TAGGTTCAGG GGCAGCAGGT TGTACTGCTG CCATCTATGC TGGCAGAGCT AATCTGAATC CCGTTTTAGT GACCGGAATG CAACCCGGTG GACAGCTGAC TATTACCACT GATGTTGAGA ATTATCCAGG CTTCTCGGCT ATAGAAGGCC CTAAGCTAAT GGAGTATATG CAGGAGCAAG CGGTAAAAAG CGGGGCTACT TTACTTACGG ACGAGATAAG TGAGATAGAC GCTAGCGTTT ATCCATTTAA GTGCACCAGT ATGTTTGGGG AAACGCTGCA AGCATACAGT ATAATAGTGG CCACTGGTGC GCAAGCTAAG TGGTTAGGTC TCGAAAGTGA GCAAAATTTT AAAAGTAAGG GTGTTTCTGC GTGTGCTACT TGTGACGGGG CCTTTTTTAA AGATGAAGTT GTTGCAGTAA TCGGTGGTGG AAATACAGCT GTTGAGGAAG CACTATATCT TACCAGAAGT TCTAAGAAGG TATTCCTCAT TCACAGAAGA GATAAACTGC GTGCTGAGGC TGTAATGCAG CAAAGGTTGT TTAGTAACAG TAAGATTGAA GTTATCTGGA ACAGCGTTGT AAAGGAAATT TTGGGTGATT CTGAGGGTAA AAAAGTAACC TCTTTGCTTT TGCAGTCTAC GGTGGATAGT ACAGAGAGCT TGCTAGACGT TGGGGGAGTA TTTGTTGCCA TAGGGCATAC ACCTAACACG CAGGTTTTGA AGAATATGCT AGGTGCTAAG GTGAATCTTG ATGATGAAGG TTATGTGATC ACTGCGCCTG GTACTACTAT CACTAGCTGT CCTGGCATAT TTGCTGCTGG AGATGTATGC GATAAAGTAT ATCGTCAAGC TGTGGTGGCT GCAGGTTCGG GATGTATGGC TGCATTGGAT GCGGAAAAGT ATCTAGCAGA GAAAGGCATC GAG
Details for AnphA.00936.a.A1.GE27005
HARVESTED ON: 7/6/2009
SEQUENCED ON: 7/21/2009
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEVHKDAV KVLILGSGAA GCTAAIYAGR ANLNPVLVTG MQPGGQLTIT TDVENYPGFS AIEGPKLMEY MQEQAVKSGA TLLTDEISEI DASVYPFKCT SMFGETLQAY SIIVATGAQA KWLGLESEQN FKSKGVSACA TCDGAFFKDE VVAVIGGGNT AVEEALYLTR SSKKVFLIHR RDKLRAEAVM QQRLFSNSKI EVIWNSVVKE ILGDSEGKKV TSLLLQSTVD STESLLDVGG VFVAIGHTPN TQVLKNMLGA KVNLDDEGYV ITAPGTTITS CPGIFAAGDV CDKVYRQAVV AAGSGCMAAL DAEKYLAEKG IE
Validated NT Sequence
ttattactcg atgcctttct ctgctagata cttttccgca tccaatgcag ccatacatcc cgaacctgca gccaccacag cttgacgata tactttatcg catacatctc cagcagcaaa tatgccagga cagctagtga tagtagtacc aggcgcagtg atcacataac cttcatcatc aagattcacc ttagcaccta gcatattctt caaaacctgc gtgttaggtg tatgccctat ggcaacaaat actcccccaa cgtctagcaa gctctctgta ctatccaccg tagactgcaa aagcaaagag gttacttttt taccctcaga atcacccaaa atttccttta caacgctgtt ccagataact tcaatcttac tgttactaaa caacctttgc tgcattacag cctcagcacg cagtttatct cttctgtgaa tgaggaatac cttcttagaa cttctggtaa gatatagtgc ttcctcaaca gctgtatttc caccaccgat tactgcaaca acttcatctt taaaaaaggc cccgtcacaa gtagcacacg cagaaacacc cttactttta aaattttgct cactttcgag acctaaccac ttagcttgcg caccagtggc cactattata ctgtatgctt gcagcgtttc cccaaacata ctggtgcact taaatggata aacgctagcg tctatctcac ttatctcgtc cgtaagtaaa gtagccccgc tttttaccgc ttgctcctgc atatactcca ttagcttagg gccttctata gccgagaagc ctggataatt ctcaacatca gtggtaatag tcagctgtcc accgggttgc attccggtca ctaaaacggg attcagatta gctctgccag catagatggc agcagtacaa cctgctgccc ctgaacctag aatcagaact ttaactgcat ctttatgcac ttctgacatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEVHKDAV KVLILGSGAA GCTAAIYAGR ANLNPVLVTG MQPGGQLTIT TDVENYPGFS AIEGPKLMEY MQEQAVKSGA TLLTDEISEI DASVYPFKCT SMFGETLQAY SIIVATGAQA KWLGLESEQN FKSKGVSACA TCDGAFFKDE VVAVIGGGNT AVEEALYLTR SSKKVFLIHR RDKLRAEAVM QQRLFSNSKI EVIWNSVVKE ILGDSEGKKV TSLLLQSTVD STESLLDVGG VFVAIGHTPN TQVLKNMLGA KVNLDDEGYV ITAPGTTITS CPGIFAAGDV CDKVYRQAVV AAGSGCMAAL DAEKYLAEKG IE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagaagtg cataaagatg cagttaaagt tctgattcta ggttcagggg cagcaggttg tactgctgcc atctatgctg gcagagctaa tctgaatccc gttttagtga ccggaatgca acccggtgga cagctgacta ttaccactga tgttgagaat tatccaggct tctcggctat agaaggccct aagctaatgg agtatatgca ggagcaagcg gtaaaaagcg gggctacttt acttacggac gagataagtg agatagacgc tagcgtttat ccatttaagt gcaccagtat gtttggggaa acgctgcaag catacagtat aatagtggcc actggtgcgc aagctaagtg gttaggtctc gaaagtgagc aaaattttaa aagtaagggt gtttctgcgt gtgctacttg tgacggggcc ttttttaaag atgaagttgt tgcagtaatc ggtggtggaa atacagctgt tgaggaagca ctatatctta ccagaagttc taagaaggta ttcctcattc acagaagaga taaactgcgt gctgaggctg taatgcagca aaggttgttt agtaacagta agattgaagt tatctggaac agcgttgtaa aggaaatttt gggtgattct gagggtaaaa aagtaacctc tttgcttttg cagtctacgg tggatagtac agagagcttg ctagacgttg ggggagtatt tgttgccata gggcatacac ctaacacgca ggttttgaag aatatgctag gtgctaaggt gaatcttgat gatgaaggtt atgtgatcac tgcgcctggt actactatca ctagctgtcc tggcatattt gctgctggag atgtatgcga taaagtatat cgtcaagctg tggtggctgc aggttcggga tgtatggctg cattggatgc ggaaaagtat ctagcagaga aaggcatcga gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.00936.a.A1.PW27045
PURIFICATION DATe: 7/21/2009
CONCENTRATION: 28.75mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 20
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEVHKDAV KVLILGSGAA GCTAAIYAGR ANLNPVLVTG MQPGGQLTIT TDVENYPGFS AIEGPKLMEY MQEQAVKSGA TLLTDEISEI DASVYPFKCT SMFGETLQAY SIIVATGAQA KWLGLESEQN FKSKGVSACA TCDGAFFKDE VVAVIGGGNT AVEEALYLTR SSKKVFLIHR RDKLRAEAVM QQRLFSNSKI EVIWNSVVKE ILGDSEGKKV TSLLLQSTVD STESLLDVGG VFVAIGHTPN TQVLKNMLGA KVNLDDEGYV ITAPGTTITS CPGIFAAGDV CDKVYRQAVV AAGSGCMAAL DAEKYLAEKG IE
Validated NT Sequence
ttattactcg atgcctttct ctgctagata cttttccgca tccaatgcag ccatacatcc cgaacctgca gccaccacag cttgacgata tactttatcg catacatctc cagcagcaaa tatgccagga cagctagtga tagtagtacc aggcgcagtg atcacataac cttcatcatc aagattcacc ttagcaccta gcatattctt caaaacctgc gtgttaggtg tatgccctat ggcaacaaat actcccccaa cgtctagcaa gctctctgta ctatccaccg tagactgcaa aagcaaagag gttacttttt taccctcaga atcacccaaa atttccttta caacgctgtt ccagataact tcaatcttac tgttactaaa caacctttgc tgcattacag cctcagcacg cagtttatct cttctgtgaa tgaggaatac cttcttagaa cttctggtaa gatatagtgc ttcctcaaca gctgtatttc caccaccgat tactgcaaca acttcatctt taaaaaaggc cccgtcacaa gtagcacacg cagaaacacc cttactttta aaattttgct cactttcgag acctaaccac ttagcttgcg caccagtggc cactattata ctgtatgctt gcagcgtttc cccaaacata ctggtgcact taaatggata aacgctagcg tctatctcac ttatctcgtc cgtaagtaaa gtagccccgc tttttaccgc ttgctcctgc atatactcca ttagcttagg gccttctata gccgagaagc ctggataatt ctcaacatca gtggtaatag tcagctgtcc accgggttgc attccggtca ctaaaacggg attcagatta gctctgccag catagatggc agcagtacaa cctgctgccc ctgaacctag aatcagaact ttaactgcat ctttatgcac ttctgacatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc atggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEVHKDAV KVLILGSGAA GCTAAIYAGR ANLNPVLVTG MQPGGQLTIT TDVENYPGFS AIEGPKLMEY MQEQAVKSGA TLLTDEISEI DASVYPFKCT SMFGETLQAY SIIVATGAQA KWLGLESEQN FKSKGVSACA TCDGAFFKDE VVAVIGGGNT AVEEALYLTR SSKKVFLIHR RDKLRAEAVM QQRLFSNSKI EVIWNSVVKE ILGDSEGKKV TSLLLQSTVD STESLLDVGG VFVAIGHTPN TQVLKNMLGA KVNLDDEGYV ITAPGTTITS CPGIFAAGDV CDKVYRQAVV AAGSGCMAAL DAEKYLAEKG IE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCAGAAGTG CATAAAGATG CAGTTAAAGT TCTGATTCTA GGTTCAGGGG CAGCAGGTTG TACTGCTGCC ATCTATGCTG GCAGAGCTAA TCTGAATCCC GTTTTAGTGA CCGGAATGCA ACCCGGTGGA CAGCTGACTA TTACCACTGA TGTTGAGAAT TATCCAGGCT TCTCGGCTAT AGAAGGCCCT AAGCTAATGG AGTATATGCA GGAGCAAGCG GTAAAAAGCG GGGCTACTTT ACTTACGGAC GAGATAAGTG AGATAGACGC TAGCGTTTAT CCATTTAAGT GCACCAGTAT GTTTGGGGAA ACGCTGCAAG CATACAGTAT AATAGTGGCC ACTGGTGCGC AAGCTAAGTG GTTAGGTCTC GAAAGTGAGC AAAATTTTAA AAGTAAGGGT GTTTCTGCGT GTGCTACTTG TGACGGGGCC TTTTTTAAAG ATGAAGTTGT TGCAGTAATC GGTGGTGGAA ATACAGCTGT TGAGGAAGCA CTATATCTTA CCAGAAGTTC TAAGAAGGTA TTCCTCATTC ACAGAAGAGA TAAACTGCGT GCTGAGGCTG TAATGCAGCA AAGGTTGTTT AGTAACAGTA AGATTGAAGT TATCTGGAAC AGCGTTGTAA AGGAAATTTT GGGTGATTCT GAGGGTAAAA AAGTAACCTC TTTGCTTTTG CAGTCTACGG TGGATAGTAC AGAGAGCTTG CTAGACGTTG GGGGAGTATT TGTTGCCATA GGGCATACAC CTAACACGCA GGTTTTGAAG AATATGCTAG GTGCTAAGGT GAATCTTGAT GATGAAGGTT ATGTGATCAC TGCGCCTGGT ACTACTATCA CTAGCTGTCC TGGCATATTT GCTGCTGGAG ATGTATGCGA TAAAGTATAT CGTCAAGCTG TGGTGGCTGC AGGTTCGGGA TGTATGGCTG CATTGGATGC GGAAAAGTAT CTAGCAGAGA AAGGCATCGA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA