AnphA.00929.a

Isoprenoid biosynthesis protein with amidotransferase-like domain

CENTER ID: AnphA.00929.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00929.a.A1.GE27004 Full length( AnphA.00929.a ) 1 221
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00929.a.A1.PW27044 Full length( AnphA.00929.a ) 1 221

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.7
RefSeq: YP_504645.1
UniProt: Q2GLV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNCAVLLCGC GHMDGSEIRE AVLALLALDS YGINVTCCAP NIKQVDVVDH LSGSTLEEER DIMSESARIA RGNVVDPKDI SPNDFDMLIL PGGFGVAKNY SDILKGESPV SVLEEVKQTI VKFHKEKKAI GAICIAPAIV AASLSSVSKV KVTLGEDIDS IISRCGGEHV FCETDDYVAD IDMGVFSTPA YMRKDSLHKI HVGIHKMVGA MVDFVKKKEQ L
NT Sequence
ATGAATTGCG CTGTACTTTT ATGTGGATGC GGGCACATGG ACGGCTCAGA GATAAGAGAG GCTGTGCTTG CTCTGTTAGC GCTTGATTCC TATGGAATAA ATGTTACTTG CTGTGCTCCT AATATTAAGC AAGTAGATGT TGTAGACCAC TTGAGTGGCT CTACTCTTGA AGAAGAAAGG GATATAATGT CGGAATCTGC AAGAATTGCT AGAGGAAACG TTGTAGACCC AAAGGATATT TCCCCTAATG ATTTTGATAT GCTAATTTTG CCTGGTGGAT TTGGAGTTGC TAAAAATTAT TCCGATATTT TGAAAGGTGA AAGTCCCGTA AGTGTGTTAG AGGAAGTAAA GCAGACAATC GTAAAGTTTC ATAAGGAAAA GAAGGCCATC GGAGCTATAT GTATTGCTCC TGCTATCGTT GCTGCTTCTC TTAGTAGTGT GTCAAAGGTA AAAGTGACAC TTGGAGAAGA CATAGATTCT ATAATATCAA GGTGCGGCGG AGAGCATGTT TTTTGCGAAA CGGACGATTA TGTAGCTGAT ATTGATATGG GTGTTTTTTC CACTCCAGCA TATATGCGTA AGGACAGCTT ACACAAAATT CACGTTGGAA TACATAAAAT GGTCGGAGCT ATGGTGGATT TTGTTAAAAA GAAAGAGCAA CTT
Details for AnphA.00929.a.A1.GE27004
HARVESTED ON: 7/6/2009
SEQUENCED ON: 7/21/2009
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNCAVLLCG CGHMDGSEIR EAVLALLALD SYGINVTCCA PNIKQVDVVD HLSGSTLEEE RDIMSESARI ARGNVVDPKD ISPNDFDMLI LPGGFGVAKN YSDILKGESP VSVLEEVKQT IVKFHKEKKA IGAICIAPAI VAASLSSVSK VKVTLGEDID SIISRCGGEH VFCETDDYVA DIDMGVFSTP AYMRKDSLHK IHVGIHKMVG AMVDFVKKK
Validated NT Sequence
ctctttcttt ttaacaaaat ccaccatagc tccgaccatt ttatgtattc caacgtgaat tttgtgtaag ctgtccttac gcatatatgc tggagtggaa aaaacaccca tatcaatatc agctacataa tcgtccgttt cgcaaaaaac atgctctccg ccgcaccttg atattataga atctatgtct tctccaagtg tcacttttac ctttgacaca ctactaagag aagcagcaac gatagcagga gcaatacata tagctccgat ggccttcttt tccttatgaa actttacgat tgtctgcttt acttcctcta acacacttac gggactttca cctttcaaaa tatcggaata atttttagca actccaaatc caccaggcaa aattagcata tcaaaatcat taggggaaat atcctttggg tctacaacgt ttcctctagc aattcttgca gattccgaca ttatatccct ttcttcttca agagtagagc cactcaagtg gtctacaaca tctacttgct taatattagg agcacagcaa gtaacattta ttccatagga atcaagcgct aacagagcaa gcacagcctc tcttatctct gagccgtcca tgtgcccgca tccacataaa agtacagcgc aattcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNCAVLLCG CGHMDGSEIR EAVLALLALD SYGINVTCCA PNIKQVDVVD HLSGSTLEEE RDIMSESARI ARGNVVDPKD ISPNDFDMLI LPGGFGVAKN YSDILKGESP VSVLEEVKQT IVKFHKEKKA IGAICIAPAI VAASLSSVSK VKVTLGEDID SIISRCGGEH VFCETDDYVA DIDMGVFSTP AYMRKDSLHK IHVGIHKMVG AMVDFVKKKE QL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaattgcgct gtacttttat gtggatgcgg gcacatggac ggctcagaga taagagaggc tgtgcttgct ctgttagcgc ttgattccta tggaataaat gttacttgct gtgctcctaa tattaagcaa gtagatgttg tagaccactt gagtggctct actcttgaag aagaaaggga tataatgtcg gaatctgcaa gaattgctag aggaaacgtt gtagacccaa aggatatttc ccctaatgat tttgatatgc taattttgcc tggtggattt ggagttgcta aaaattattc cgatattttg aaaggtgaaa gtcccgtaag tgtgttagag gaagtaaagc agacaatcgt aaagtttcat aaggaaaaga aggccatcgg agctatatgt attgctcctg ctatcgttgc tgcttctctt agtagtgtgt caaaggtaaa agtgacactt ggagaagaca tagattctat aatatcaagg tgcggcggag agcatgtttt ttgcgaaacg gacgattatg tagctgatat tgatatgggt gttttttcca ctccagcata tatgcgtaag gacagcttac acaaaattca cgttggaata cataaaatgg tcggagctat ggtggatttt gttaaaaaga aagagcaact taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.00929.a.A1.PW27044
PURIFICATION DATe: 7/21/2009
CONCENTRATION: 27.4mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 39
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNCAVLLCG CGHMDGSEIR EAVLALLALD SYGINVTCCA PNIKQVDVVD HLSGSTLEEE RDIMSESARI ARGNVVDPKD ISPNDFDMLI LPGGFGVAKN YSDILKGESP VSVLEEVKQT IVKFHKEKKA IGAICIAPAI VAASLSSVSK VKVTLGEDID SIISRCGGEH VFCETDDYVA DIDMGVFSTP AYMRKDSLHK IHVGIHKMVG AMVDFVKKK
Validated NT Sequence
ctctttcttt ttaacaaaat ccaccatagc tccgaccatt ttatgtattc caacgtgaat tttgtgtaag ctgtccttac gcatatatgc tggagtggaa aaaacaccca tatcaatatc agctacataa tcgtccgttt cgcaaaaaac atgctctccg ccgcaccttg atattataga atctatgtct tctccaagtg tcacttttac ctttgacaca ctactaagag aagcagcaac gatagcagga gcaatacata tagctccgat ggccttcttt tccttatgaa actttacgat tgtctgcttt acttcctcta acacacttac gggactttca cctttcaaaa tatcggaata atttttagca actccaaatc caccaggcaa aattagcata tcaaaatcat taggggaaat atcctttggg tctacaacgt ttcctctagc aattcttgca gattccgaca ttatatccct ttcttcttca agagtagagc cactcaagtg gtctacaaca tctacttgct taatattagg agcacagcaa gtaacattta ttccatagga atcaagcgct aacagagcaa gcacagcctc tcttatctct gagccgtcca tgtgcccgca tccacataaa agtacagcgc aattcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNCAVLLCG CGHMDGSEIR EAVLALLALD SYGINVTCCA PNIKQVDVVD HLSGSTLEEE RDIMSESARI ARGNVVDPKD ISPNDFDMLI LPGGFGVAKN YSDILKGESP VSVLEEVKQT IVKFHKEKKA IGAICIAPAI VAASLSSVSK VKVTLGEDID SIISRCGGEH VFCETDDYVA DIDMGVFSTP AYMRKDSLHK IHVGIHKMVG AMVDFVKKKE QL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATTGCGCT GTACTTTTAT GTGGATGCGG GCACATGGAC GGCTCAGAGA TAAGAGAGGC TGTGCTTGCT CTGTTAGCGC TTGATTCCTA TGGAATAAAT GTTACTTGCT GTGCTCCTAA TATTAAGCAA GTAGATGTTG TAGACCACTT GAGTGGCTCT ACTCTTGAAG AAGAAAGGGA TATAATGTCG GAATCTGCAA GAATTGCTAG AGGAAACGTT GTAGACCCAA AGGATATTTC CCCTAATGAT TTTGATATGC TAATTTTGCC TGGTGGATTT GGAGTTGCTA AAAATTATTC CGATATTTTG AAAGGTGAAA GTCCCGTAAG TGTGTTAGAG GAAGTAAAGC AGACAATCGT AAAGTTTCAT AAGGAAAAGA AGGCCATCGG AGCTATATGT ATTGCTCCTG CTATCGTTGC TGCTTCTCTT AGTAGTGTGT CAAAGGTAAA AGTGACACTT GGAGAAGACA TAGATTCTAT AATATCAAGG TGCGGCGGAG AGCATGTTTT TTGCGAAACG GACGATTATG TAGCTGATAT TGATATGGGT GTTTTTTCCA CTCCAGCATA TATGCGTAAG GACAGCTTAC ACAAAATTCA CGTTGGAATA CATAAAATGG TCGGAGCTAT GGTGGATTTT GTTAAAAAGA AAGAGCAACT TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA