AnphA.00921.a

2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase

CENTER ID:	AnphA.00921.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00921.a.A1.GE27002	Full length( AnphA.00921.a )		1	176

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_133110
PATRIC ID:	fig\|212042.8.peg.224
RefSeq:	YP_504833.1
UniProt:	Q2GLB8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVKQIYQPNK SAMVVLALGS NIGDRMSALK EAIKLIPLHN RLYSSVYESC ALLPDNAPFC WNMPFLNMVI AGYTHFSPED LLQSIKQIES QLGRCSLGHW SPRSIDIDII LWEGVTVEDK ALTIPHKEMH KRDFVLVPTC DICPRFPHPI LKETVESILL NKQDINLVKK YQAIHL

NT Sequence

ATGGTTAAAC AAATATATCA ACCCAACAAA AGTGCTATGG TTGTATTAGC ATTGGGCAGC AATATTGGCG ATAGGATGAG CGCTTTAAAA GAAGCAATAA AACTCATACC GTTGCATAAT AGACTATACT CATCCGTATA TGAGAGTTGT GCTTTGCTTC CTGATAACGC CCCATTTTGC TGGAACATGC CTTTTCTAAA CATGGTCATC GCGGGATATA CACATTTTTC TCCAGAAGAT CTCTTACAAA GCATTAAACA AATAGAATCC CAGCTAGGAA GATGTAGTCT TGGTCATTGG AGCCCTAGAA GCATAGATAT AGACATAATA TTGTGGGAAG GCGTTACAGT AGAGGATAAA GCTCTCACTA TACCTCATAA GGAAATGCAC AAACGTGACT TTGTTCTAGT ACCTACCTGC GACATATGTC CCCGTTTTCC ACATCCTATA CTAAAAGAAA CAGTAGAATC GATATTGCTA AATAAGCAGG ATATCAACCT TGTGAAAAAG TATCAGGCTA TACACTTA

Details for AnphA.00921.a.A1.GE27002

HARVESTED ON:	7/6/2009
SEQUENCED ON:	7/21/2009
EXPECTED MW:	22kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MGTLEAQTQG PGSMVKQIYQ PNKSAMVVLA LGSNIGDRMS ALKEAIKLIP LHNRLYSSVY ESCALLPDNA PFCWNMPFLN MVIAGYTHFS PEDLLQSIKQ IESQLGRCSL GHWSPRSIDI DIILWEGVTV EDKALTIPHK EMHKRDFVLV PTCDICPRFP HPILKETVES ILLNKQDINL VKKYQA

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggttaa acaaatatat caacccaaca aaagtgctat ggttgtatta gcattgggca gcaatattgg cgataggatg agcgctttaa aagaagcaat aaaactcata ccgttgcata atagactata ctcatccgta tatgagagtt gtgctttgct tcctgataac gccccatttt gctggaacat gccttttcta aacatggtca tcgcgggata tacacatttt tctccagaag atctcttaca aagcattaaa caaatagaat cccagctagg aagatgtagt cttggtcatt ggagccctag aagcatagat atagacataa tattgtggga aggcgttaca gtagaggata aagctctcac tatacctcat aaggaaatgc acaaacgtga ctttgttcta gtacctacct gcgacatatg tccccgtttt ccacatccta tactaaaaga aacagtagaa tcgatattgc taaataagca ggatatcaac cttgtgaaaa agtatcaggc tata

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVKQIYQPN KSAMVVLALG SNIGDRMSAL KEAIKLIPLH NRLYSSVYES CALLPDNAPF CWNMPFLNMV IAGYTHFSPE DLLQSIKQIE SQLGRCSLGH WSPRSIDIDI ILWEGVTVED KALTIPHKEM HKRDFVLVPT CDICPRFPHP ILKETVESIL LNKQDINLVK KYQAIHL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttaaacaa atatatcaac ccaacaaaag tgctatggtt gtattagcat tgggcagcaa tattggcgat aggatgagcg ctttaaaaga agcaataaaa ctcataccgt tgcataatag actatactca tccgtatatg agagttgtgc tttgcttcct gataacgccc cattttgctg gaacatgcct tttctaaaca tggtcatcgc gggatataca catttttctc cagaagatct cttacaaagc attaaacaaa tagaatccca gctaggaaga tgtagtcttg gtcattggag ccctagaagc atagatatag acataatatt gtgggaaggc gttacagtag aggataaagc tctcactata cctcataagg aaatgcacaa acgtgacttt gttctagtac ctacctgcga catatgtccc cgttttccac atcctatact aaaagaaaca gtagaatcga tattgctaaa taagcaggat atcaaccttg tgaaaaagta tcaggctata cacttaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa