AnphA.00847.b

8-amino-7-oxononanoate synthase

CENTER ID:	AnphA.00847.b
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00847.b.A1.GE34601	Full length( AnphA.00847.b )		1	378

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126783
PATRIC ID:	fig\|212042.8.peg.1087
RefSeq:	YP_505577.1
UniProt:	Q2GJ74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKDILRERL EVIESNGLLR KLKQSTIQSS IAGRKIQNDA GNELTSFSCN DYMGLSTHDV VKQAAIDAIN LYGMGARASR LTTGNHPLYA EIEAKLASLY GTEAALVFSS GYTTNIGSIS ALVGRHDMIL ADKFIHASSL DGARLSGATI YRFTHNNVDH CMQLISKYRE LHDNCLILLE NIYGVDGDLA PVDEFIQLAK EWNAWVIVDT AHGFGMLSSL QADIYVGTLS KAMGALGGFV CSSKITIEYL LNKSRSFIYT TALPPAIIAA ASASIDLFKS YAEVPMRLAK EFCKIMELPE PQSHIVPLIM EDVRSVILAE QALAERGILV SAIRPPTAPT PRLRLTFTAA HKLIDVHRLC NALKACVVLT EAGNWRVL

NT Sequence

ATGTTAAAAG ATATATTAAG AGAGAGGTTA GAGGTAATAG AGAGCAATGG ATTACTTAGA AAGTTAAAGC AAAGCACAAT ACAGTCGTCA ATAGCGGGAC GTAAAATACA AAATGATGCA GGAAATGAGC TCACTTCTTT CTCATGCAAT GATTACATGG GGCTTTCAAC ACATGATGTA GTAAAGCAAG CCGCAATAGA TGCTATCAAT TTGTATGGCA TGGGTGCACG CGCCTCTAGA TTGACCACAG GAAACCATCC ATTGTACGCA GAAATAGAAG CAAAACTTGC ATCACTATAC GGAACAGAAG CTGCGTTGGT ATTTAGCAGC GGATATACAA CAAATATAGG AAGCATCTCA GCATTGGTAG GTCGCCATGA TATGATCTTG GCAGATAAGT TCATACACGC TTCTTCTCTT GATGGAGCAA GATTATCTGG AGCTACAATA TACAGGTTTA CGCACAATAA CGTAGATCAC TGCATGCAAC TAATTAGTAA GTATAGGGAA CTACACGATA ACTGCCTAAT TCTGCTAGAA AACATATACG GGGTAGATGG TGACTTAGCT CCCGTAGACG AGTTTATCCA ACTAGCAAAA GAGTGGAATG CTTGGGTCAT TGTAGATACT GCTCATGGCT TTGGTATGCT CAGTTCCTTA CAAGCAGACA TATATGTGGG TACACTTTCC AAGGCAATGG GAGCACTGGG TGGATTTGTG TGCTCATCAA AAATTACGAT TGAGTACTTA TTAAATAAGT CACGCAGTTT TATTTATACA ACTGCTTTAC CACCGGCAAT TATAGCAGCT GCAAGTGCGT CTATAGACCT TTTCAAATCT TACGCAGAAG TTCCGATGCG TCTTGCAAAA GAATTTTGCA AAATTATGGA ACTACCTGAA CCGCAAAGTC ACATAGTGCC TTTGATTATG GAAGATGTCA GAAGCGTGAT ACTTGCTGAG CAAGCCCTAG CTGAACGGGG CATTTTAGTA TCAGCAATTC GTCCTCCGAC AGCCCCAACT CCGAGACTGA GGTTAACATT TACTGCTGCA CACAAGCTAA TAGACGTACA TAGACTGTGT AATGCATTGA AAGCGTGCGT TGTACTTACA GAGGCAGGAA ACTGGCGGGT CCTA

Details for AnphA.00847.b.A1.GE34601

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	75

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKDILRER LEVIESNGLL RKLKQSTIQS SIAGRKIQND AGNELTSFSC NDYMGLSTHD VVKQAAIDAI NLYGMGARAS RLTTGNHPLY AEIEAKLASL YGTEAALVFS SGYTTNIGSI SALVGRHDMI LADKFIHASS LDGARLSGAT IYRFTHNNVD HCMQLISKYR ELHDNCLILL ENIYGVDGDL APVDEFIXLA XEWNAWVIVD TAHGFGMLSS LQADIYVGTL SKAMGALGGF VCSSKITIEY LLNKSRSFIY TTALPPAIIA AASASIDLFK SYA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttaa aagatatatt aagagagagg ttagaggtaa tagagagcaa tggattactt agaaagttaa agcaaagcac aatacagtcg tcaatagcgg gacgtaaaat acaaaatgat gcaggaaatg agctcacttc tttctcatgc aatgattaca tggggctttc aacacatgat gtagtaaagc aagccgcaat agatgctatc aatttgtatg gcatgggtgc acgcgcctct agattgacca caggaaacca tccattgtac gcagaaatag aagcaaaact tgcatcacta tacggaacag aagctgcgtt ggtatttagc agcggatata caacaaatat aggaagcatc tcagcattgg taggtcgcca tgatatgatc ttggcagata agttcataca cgcttcttct cttgatggag caagattatc tggagctaca atatacaggt ttacgcacaa taacgtagat cactgcatgc aactaattag taagtatagg gaactacacg ataactgcct aattctgcta gaaaacatat acggggtaga tggtgactta gctcccgtag acgagtttat ccnactagca naagagtgga atgcttgggt cattgtagat actgctcatg gctttggtat gctcagttcc ttacaagcag acatatatgt gggtacactt tccaaggcaa tgggagcact gggtggattt gtgtgctcat caaaaattac gattgagtac ttattaaata agtcacgcag ttttatttat acnactgctt taccaccggc aattatagca gctgcaagtg cgtctataga ccttttcaaa tcttacgcan aa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKDILRER LEVIESNGLL RKLKQSTIQS SIAGRKIQND AGNELTSFSC NDYMGLSTHD VVKQAAIDAI NLYGMGARAS RLTTGNHPLY AEIEAKLASL YGTEAALVFS SGYTTNIGSI SALVGRHDMI LADKFIHASS LDGARLSGAT IYRFTHNNVD HCMQLISKYR ELHDNCLILL ENIYGVDGDL APVDEFIQLA KEWNAWVIVD TAHGFGMLSS LQADIYVGTL SKAMGALGGF VCSSKITIEY LLNKSRSFIY TTALPPAIIA AASASIDLFK SYAEVPMRLA KEFCKIMELP EPQSHIVPLI MEDVRSVILA EQALAERGIL VSAIRPPTAP TPRLRLTFTA AHKLIDVHRL CNALKACVVL TEAGNWRVL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttaaaagat atattaagag agaggttaga ggtaatagag agcaatggat tacttagaaa gttaaagcaa agcacaatac agtcgtcaat agcgggacgt aaaatacaaa atgatgcagg aaatgagctc acttctttct catgcaatga ttacatgggg ctttcaacac atgatgtagt aaagcaagcc gcaatagatg ctatcaattt gtatggcatg ggtgcacgcg cctctagatt gaccacagga aaccatccat tgtacgcaga aatagaagca aaacttgcat cactatacgg aacagaagct gcgttggtat ttagcagcgg atatacaaca aatataggaa gcatctcagc attggtaggt cgccatgata tgatcttggc agataagttc atacacgctt cttctcttga tggagcaaga ttatctggag ctacaatata caggtttacg cacaataacg tagatcactg catgcaacta attagtaagt atagggaact acacgataac tgcctaattc tgctagaaaa catatacggg gtagatggtg acttagctcc cgtagacgag tttatccaac tagcaaaaga gtggaatgct tgggtcattg tagatactgc tcatggcttt ggtatgctca gttccttaca agcagacata tatgtgggta cactttccaa ggcaatggga gcactgggtg gatttgtgtg ctcatcaaaa attacgattg agtacttatt aaataagtca cgcagtttta tttatacaac tgctttacca ccggcaatta tagcagctgc aagtgcgtct atagaccttt tcaaatctta cgcagaagtt ccgatgcgtc ttgcaaaaga attttgcaaa attatggaac tacctgaacc gcaaagtcac atagtgcctt tgattatgga agatgtcaga agcgtgatac ttgctgagca agccctagct gaacggggca ttttagtatc agcaattcgt cctccgacag ccccaactcc gagactgagg ttaacattta ctgctgcaca caagctaata gacgtacata gactgtgtaa tgcattgaaa gcgtgcgttg tacttacaga ggcaggaaac tggcgggtcc taaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa