AnphA.00847.a

8-amino-7-oxononanoate synthase, truncation

CENTER ID:	AnphA.00847.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00847.a.A1.GE34598	Full length( AnphA.00847.a )		1	273

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126783
PATRIC ID:	fig\|212042.8.peg.1042
RefSeq:	YP_505544.1
UniProt:	Q2GJA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVFSSGYTTN IGSISALVGR HDMILADKFI HASSLDGARL SGATIYRFTH NNVDHCMQLI SKYRELHDNC LILLENIYGV DGDLAPVDEF IQLAKEWNAW VIVDTAHGFG MLSSLQADIY VGTLSKAMGA LGGFVCSSKI TIEYLLNKSR SFIYTTALPP AIIAAASASI DLFKSYAEVP MRLAKEFCKI MELPEPQSHI VPLIMEDVRS VILAEQALAE RGILVSAIRP PTAPTPRLRL TFTAAHKLID VHRLCNALKA CVVLTEAGNW RVL

NT Sequence

TTGGTATTTA GCAGCGGATA TACAACAAAT ATAGGAAGCA TCTCAGCATT GGTAGGTCGC CATGATATGA TCTTGGCAGA TAAGTTCATA CACGCTTCTT CTCTTGATGG AGCAAGATTA TCTGGAGCTA CAATATACAG GTTTACGCAC AATAACGTAG ATCACTGCAT GCAACTAATT AGTAAGTATA GGGAACTACA CGATAACTGC CTAATTCTGC TAGAAAACAT ATACGGGGTA GATGGTGACT TAGCTCCCGT AGACGAGTTT ATCCAACTAG CAAAAGAGTG GAATGCTTGG GTCATTGTAG ATACTGCTCA TGGCTTTGGT ATGCTCAGTT CCTTACAAGC AGACATATAT GTGGGTACAC TTTCCAAGGC AATGGGGGCA CTGGGTGGAT TTGTGTGCTC ATCAAAAATT ACGATTGAGT ACTTATTAAA TAAGTCACGC AGTTTTATTT ATACAACTGC TTTACCACCG GCAATTATAG CAGCTGCAAG TGCGTCTATA GACCTTTTCA AATCTTACGC AGAAGTTCCG ATGCGTCTTG CAAAAGAATT TTGCAAAATT ATGGAACTAC CTGAACCGCA AAGTCACATA GTGCCTTTGA TTATGGAAGA TGTCAGAAGC GTGATACTTG CTGAGCAAGC CCTAGCTGAA CGGGGCATTT TAGTATCAGC AATTCGTCCT CCGACAGCCC CAACTCCGAG ACTGAGGTTA ACATTTACTG CTGCACACAA GCTAATAGAC GTACATAGAC TGTGTAATGC ATTGAAAGCG TGCGTTGTAC TTACAGAGGC AGGAAACTGG CGGGTCCTA

Details for AnphA.00847.a.A1.GE34598

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	32kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLVFSSGYT TNIGSISALV GRHDMILADK FIHASSLDGA RLSGATIYRF THNNVDHCMQ LISKYRELHD NCLILLENIY GVDGDLAPVD EFIQLAKEWN AWVIVDTAHG FGMLSSLQAD IYVGTLSKAM GALGGFVCSS KITIEYLLNK SRSFIYTTAL PPAIIAAASA SIDLFKSYAE VPMRLAKEFC KIMELPEPQS HIVPLIMEDV RSVILAEQAL AERGILVSAI RPPTAPTPRL RLTFTAAHKL IDVHRLCNAL KACVVLTEAG NWRVL

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg tatttagcag cggatataca acaaatatag gaagcatctc agcattggta ggtcgccatg atatgatctt ggcagataag ttcatacacg cttcttctct tgatggagca agattatctg gagctacaat atacaggttt acgcacaata acgtagatca ctgcatgcaa ctaattagta agtataggga actacacgat aactgcctaa ttctgctaga aaacatatac ggggtagatg gtgacttagc tcccgtagac gagtttatcc aactagcaaa agagtggaat gcttgggtca ttgtagatac tgctcatggc tttggtatgc tcagttcctt acaagcagac atatatgtgg gtacactttc caaggcaatg ggggcactgg gtggatttgt gtgctcatca aaaattacga ttgagtactt attaaataag tcacgcagtt ttatttatac aactgcttta ccaccggcaa ttatagcagc tgcaagtgcg tctatagacc ttttcaaatc ttacgcagaa gttccgatgc gtcttgcaaa agaattttgc aaaattatgg aactacctga accgcaaagt cacatagtgc ctttgattat ggaagatgtc agaagcgtga tacttgctga gcaagcccta gctgaacggg gcattttagt atcagcaatt cgtcctccga cagccccaac tccgagactg aggttaacat ttactgctgc acacaagcta atagacgtac atagactgtg taatgcattg aaagcgtgcg ttgtacttac agaggcagga aattggcggg tcctataata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggtattt agcagcggat atacaacaaa tataggaagc atctcagcat tggtaggtcg ccatgatatg atcttggcag ataagttcat acacgcttct tctcttgatg gagcaagatt atctggagct acaatataca ggtttacgca caataacgta gatcactgca tgcaactaat tagtaagtat agggaactac acgataactg cctaattctg ctagaaaaca tatacggggt agatggtgac ttagctcccg tagacgagtt tatccaacta gcaaaagagt ggaatgcttg ggtcattgta gatactgctc atggctttgg tatgctcagt tccttacaag cagacatata tgtgggtaca ctttccaagg caatgggggc actgggtgga tttgtgtgct catcaaaaat tacgattgag tacttattaa ataagtcacg cagttttatt tatacaactg ctttaccacc ggcaattata gcagctgcaa gtgcgtctat agaccttttc aaatcttacg cagaagttcc gatgcgtctt gcaaaagaat tttgcaaaat tatggaacta cctgaaccgc aaagtcacat agtgcctttg attatggaag atgtcagaag cgtgatactt gctgagcaag ccctagctga acggggcatt ttagtatcag caattcgtcc tccgacagcc ccaactccga gactgaggtt aacatttact gctgcacaca agctaataga cgtacataga ctgtgtaatg cattgaaagc gtgcgttgta cttacagagg caggaaactg gcgggtccta aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa