AnphA.00846.a

50S ribosomal protein L4

CENTER ID:	AnphA.00846.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00846.a.A1.GE26687	Full length( AnphA.00846.a )		1	208

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127316
PATRIC ID:	fig\|212042.8.peg.290
RefSeq:	YP_504893.1
UniProt:	Q2GL58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEVNIVNIAN EVVGSIDLNP QVFGLEPRRD VLKEVVNWQL AKRRAGTHKA KGISDISGTT AKPYRQKHTG RARQGSLRSP QFRGGAVIFG PVPRSHEYSL NKKVRRLGLK VALSMKVAAN KLVVLDSLDV DLKKTADAKG VFGNFAGHSS YLVVSESCKE EVVRACRNLR GVDLLKCVGI NVLDILKHDC VILTVDTVKS LEGRLSNE

NT Sequence

ATGGAAGTTA ATATTGTTAA CATAGCGAAT GAGGTTGTAG GTAGTATTGA CCTGAATCCG CAGGTGTTTG GGCTTGAGCC AAGGCGCGAT GTGCTTAAAG AGGTCGTAAA CTGGCAGCTT GCTAAGAGGC GTGCTGGGAC TCATAAAGCT AAGGGTATTA GTGATATTTC AGGTACTACT GCGAAGCCTT ATAGGCAGAA GCATACTGGT AGGGCGAGGC AGGGTAGTTT GCGTAGTCCT CAATTTAGGG GTGGTGCGGT GATATTTGGG CCAGTGCCTC GTAGTCATGA GTATTCTTTA AATAAGAAGG TTCGTAGATT GGGGCTCAAG GTAGCTTTGT CGATGAAGGT TGCTGCTAAT AAGCTAGTGG TGCTTGATAG TCTAGATGTG GATCTCAAGA AAACCGCGGA TGCCAAGGGG GTCTTTGGTA ATTTTGCGGG GCATAGTTCG TATTTGGTGG TCTCTGAAAG TTGTAAGGAA GAGGTTGTGC GGGCTTGTCG TAATTTGCGG GGTGTAGACT TATTAAAGTG TGTTGGTATA AATGTTTTAG ACATACTGAA GCATGATTGT GTGATATTAA CTGTGGATAC AGTGAAGTCT CTTGAGGGTA GGTTGAGCAA TGAG

Details for AnphA.00846.a.A1.GE26687

HARVESTED ON:	5/15/2009
SEQUENCED ON:	6/18/2009
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MGTLEAQTQG PGSMEVNIVN IANEVVGSID LNPQVFGLEP RRDVLKEVVN WQLAKRRAGT HKAKGISDIS GTTAKPYRQK HTGRARQGSL RSPQFRGGAV IFGPVPRSHE YSLNKKVRRL GLKVALSMKV AANKLVVLDS LDVDLKKTAD AKGVFGNFAG HSSYLVVSES CKEEVVRACR NLRGVDLLKC VGINVLDILK HDCVILTVDT VKSLEGRLS

Validated NT Sequence

tgctcaacct accctcaaga gacttcactg tatccacagt taatatcaca caatcatgct tcagtatgtc taaaacattt ataccaacac actttaataa gtctacaccc cgcaaattac gacaagcccg cacaacctct tccttacaac tttcagagac caccaaatac gaactatgcc ccgcaaaatt accaaagacc cccttggcat ccgcggtttt cttgagatcc acatctagac tatcaagcac cactagctta ttagcagcaa ccttcatcga caaagctacc ttgagcccca atctacgaac cttcttattt aaagaatact catgactacg aggcactggc ccaaatatca ccgcaccacc cctaaattga ggactacgca aactaccctg cctcgcccta ccagtatgct tctgcctata aggcttcgca gtagtacctg aaatatcact aataccctta gctttatgag tcccagcacg cctcttagca agctgccagt ttacgacctc tttaagcaca tcgcgccttg gctcaagccc aaacacctgc ggattcaggt caatactacc tacaacctca ttcgctatgt taacaatatt aacttccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgang gngatgagcc anggta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEVNIVNIA NEVVGSIDLN PQVFGLEPRR DVLKEVVNWQ LAKRRAGTHK AKGISDISGT TAKPYRQKHT GRARQGSLRS PQFRGGAVIF GPVPRSHEYS LNKKVRRLGL KVALSMKVAA NKLVVLDSLD VDLKKTADAK GVFGNFAGHS SYLVVSESCK EEVVRACRNL RGVDLLKCVG INVLDILKHD CVILTVDTVK SLEGRLSNE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaagttaat attgttaaca tagcgaatga ggttgtaggt agtattgacc tgaatccgca ggtgtttggg cttgagccaa ggcgcgatgt gcttaaagag gtcgtaaact ggcagcttgc taagaggcgt gctgggactc ataaagctaa gggtattagt gatatttcag gtactactgc gaagccttat aggcagaagc atactggtag ggcgaggcag ggtagtttgc gtagtcctca atttaggggt ggtgcggtga tatttgggcc agtgcctcgt agtcatgagt attctttaaa taagaaggtt cgtagattgg ggctcaaggt agctttgtcg atgaaggttg ctgctaataa gctagtggtg cttgatagtc tagatgtgga tctcaagaaa accgcggatg ccaagggggt ctttggtaat tttgcggggc atagttcgta tttggtggtc tctgaaagtt gtaaggaaga ggttgtgcgg gcttgtcgta atttgcgggg tgtagactta ttaaagtgtg ttggtataaa tgttttagac atactgaagc atgattgtgt gatattaact gtggatacag tgaagtctct tgagggtagg ttgagcaatg agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa