AnphA.00818.a

ABC transporter, ATP-binding protein

CENTER ID:	AnphA.00818.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00818.a.B1.GE26917	Full length( AnphA.00818.a )		1	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131675
PATRIC ID:	fig\|212042.8.peg.4
RefSeq:	YP_504643.1
UniProt:	Q2GLV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYAVSLSNLH VSFNNKEVLK GVDLDIAWGD SLVILGESGS GKSVLTKVVL GLIVPQEGSV TVDGTNILEN RQGIKNFSVL FQNCALFDSL TIWENVVFNF RRRLRLDKDN AKALALRGLE LVGLDASVMN VYPVELSGGM KKRVALARAI IGSPKILILD EPTSGLDPIM SSVVSDIIAR CHRDFNLTII TITHDLSSAF HVANKIAVLR GGKIIACDGV ENIKKSTDPY VAKFMKFGMH T

NT Sequence

ATGTACGCTG TATCTCTTTC AAATTTGCAT GTCTCTTTCA ACAACAAGGA GGTTTTGAAA GGTGTTGACT TGGACATAGC ATGGGGGGAT TCCCTGGTTA TACTGGGAGA ATCTGGTAGT GGAAAGTCTG TACTAACAAA GGTTGTATTG GGTCTAATAG TGCCCCAAGA GGGAAGTGTT ACTGTAGATG GCACCAATAT TCTTGAGAAT AGGCAGGGCA TCAAGAATTT TAGTGTTTTG TTTCAAAACT GTGCGTTATT TGACAGTCTT ACGATTTGGG AAAATGTAGT ATTCAATTTC CGTAGGAGGC TTCGTTTAGA TAAGGATAAT GCCAAGGCTT TGGCTTTACG GGGATTGGAG CTTGTGGGAT TGGACGCCAG TGTAATGAAC GTGTATCCTG TGGAGCTATC AGGCGGGATG AAAAAGCGCG TAGCTTTGGC AAGAGCTATT ATAGGTAGTC CCAAAATTCT AATTTTGGAT GAGCCAACTT CGGGATTGGA TCCTATAATG TCTTCAGTGG TTAGTGACAT AATTGCGCGA TGTCACAGGG ATTTCAACCT TACTATTATT ACCATTACGC ATGATCTTAG CAGTGCATTT CACGTTGCTA ACAAAATTGC TGTATTACGG GGTGGTAAAA TCATTGCATG TGATGGAGTA GAGAACATAA AAAAGAGTAC TGATCCGTAT GTAGCCAAGT TTATGAAATT CGGAATGCAC ACG

Details for AnphA.00818.a.B1.GE26917

HARVESTED ON:	6/18/2009
SEQUENCED ON:	7/15/2009
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMY AVSLSNLHVS FNNKEVLKGV DLDIAWGDSL VILGESGSGK SVLTKVVLGL IVPQEGSVTV DGTNILENRQ GIKNFSVLFQ NCALFDSLTI WENVVFNFRR RLRLDKDNAK ALALRGLELV GLDASVMNVY PVELSGGMKK RVALARAIIG SPKILILDEP TSGLDPIMSS VVSDIIARCH RDFNLTIITI THDLSSAFHV ANKIAVLRGG KIIACDGVEN IKKSTDPYVA KFMKFGMHT

Validated NT Sequence

atcttactca cttacgtgtg cattccgaat ttcataaact tggctacata cggatcagta ctctttttta tgttctctac tccatcacat gcaatgattt taccaccccg taatacagca attttgttag caacgtgaaa tgcactgcta agatcatgcg taatggtaat aatagtaagg ttgaaatccc tgtgacatcg cgcaattatg tcactaacca ctgaagacat tataggatcc aatcccgaag ttggctcatc caaaattaga attttgggac tacctataat agctcttgcc aaagctacgc gctttttcat cccgcctgat agctccacag gatacacgtt cattacactg gcgtccaatc ccacaagctc caatccccgt aaagccaaag ccttggcatt atccttatct aaacgaagcc tcctacggaa attgaatact acattttccc aaatcgtaag actgtcaaat aacgcacagt tttgaaacaa aacactaaaa ttcttgatgc cctgcctatt ctcaagaata ttggtgccat ctacagtaac acttccctct tggggcacta ttagacccaa tacaaccttt gttagtacag actttccact accagattct cccagtataa ccagggaatc cccccatgct atgtccaagt caacaccttt caaaacctcc ttgttgttga aagagacatg caaatttgaa agagatacag cgtacatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtacg ctgtatctct ttcaaatttg catgtctctt tcaacaacaa ggaggttttg aaaggtgttg acttggacat agcatggggg gattccctgg ttatactggg agaatctggt agtggaaagt ctgtactaac aaaggttgta ttgggtctaa tagtgcccca agagggaagt gttactgtag atggcaccaa tattcttgag aataggcagg gcatcaagaa ttttagtgtt ttgtttcaaa actgtgcgtt atttgacagt cttacgattt gggaaaatgt agtattcaat ttccgtagga ggcttcgttt agataaggat aatgccaagg ctttggcttt acggggattg gagcttgtgg gattggacgc cagtgtaatg aacgtgtatc ctgtggagct atcaggcggg atgaaaaagc gcgtagcttt ggcaagagct attataggta gtcccaaaat tctaattttg gatgagccaa cttcgggatt ggatcctata atgtcttcag tggttagtga cataattgcg cgatgtcaca gggatttcaa ccttactatt attaccatta cgcatgatct tagcagtgca tttcacgttg ctaacaaaat tgctgtatta cggggtggta aaatcattgc atgtgatgga gtagagaaca taaaaaagag tactgatccg tatgtagcca agtttatgaa attcggaatg cacacgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t