AnphA.00817.a
enoyl-(acyl-carrier-protein) reductase
| CENTER ID: | AnphA.00817.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.00817.a.A1.GE26660 | Full length( AnphA.00817.a ) | 1 | 275 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.00817.a.A1.PS00247 | Full length( AnphA.00817.a ) | 1 | 275 | ||
| AnphA.00817.a.A1.PS00248 | Full length( AnphA.00817.a ) | 1 | 275 |
Structures
3K2E
DEPOSITED: 9/30/2009
DETERMINATION: XRay
CLONE: AnphA.00817.a.A1.GE26660
PROTEIN: AnphA.00817.a.A1.PS00248
3K31
DEPOSITED: 9/30/2009
DETERMINATION: XRay
CLONE: AnphA.00817.a.A1.GE26660
PROTEIN: AnphA.00817.a.A1.PS00248
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130466 |
| PATRIC ID: | fig|212042.8.peg.508 |
| RefSeq: | YP_505073.1 |
| UniProt: | Q2GKM8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTGMLMEGK KGVIIGVAND KSLAWGIAKA VCAQGAEVAL TYLSETFKKR VDPLAESLGV KLTVPCDVSD AESVDNMFKV LAEEWGSLDF VVHAVAFSDK NELKGRYVDT SLGNFLTSMH ISCYSFTYIA SKAEPLMTNG GSILTLSYYG AEKVVPHYNV MGVCKAALEA SVKYLAVDLG KQQIRVNAIS AGPVRTLASS GISDFHYILT WNKYNSPLRR NTTLDDVGGA ALYLLSDLGR GTTGETVHVD CGYHVVGMKS VDAPDISRVK GDHSV
NT Sequence
GTGCGTACCG GCATGCTCAT GGAAGGAAAA AAGGGAGTCA TCATTGGTGT AGCTAATGAT AAGTCCTTGG CGTGGGGCAT CGCGAAAGCT GTGTGTGCAC AGGGTGCCGA GGTTGCTCTT ACATATCTTT CGGAGACTTT TAAGAAAAGG GTAGATCCCC TTGCGGAGTC TCTAGGTGTT AAGTTGACGG TTCCGTGTGA TGTATCAGAT GCGGAATCTG TAGACAATAT GTTCAAAGTT CTTGCTGAGG AGTGGGGTAG TCTGGATTTT GTGGTCCACG CGGTTGCATT TTCCGATAAG AACGAGCTCA AGGGAAGGTA TGTTGATACT TCTCTGGGTA ACTTCCTGAC GTCGATGCAT ATATCGTGTT ATTCTTTTAC TTACATTGCG AGTAAAGCTG AGCCACTGAT GACCAATGGT GGCAGTATTT TGACTCTCTC TTATTACGGT GCTGAGAAGG TAGTGCCCCA TTACAATGTG ATGGGCGTGT GCAAGGCGGC CCTCGAGGCA AGTGTTAAAT ATTTGGCGGT TGATTTGGGT AAGCAGCAGA TAAGAGTTAA TGCTATTTCT GCGGGGCCTG TTAGAACTTT GGCGTCTTCT GGAATTAGTG ATTTTCATTA TATACTGACG TGGAATAAGT ATAATTCGCC GTTGAGGCGC AATACTACTC TTGATGATGT CGGGGGGGCT GCGTTATATC TGCTGAGTGA TTTGGGTAGA GGGACAACTG GTGAGACTGT GCATGTGGAT TGTGGGTACC ATGTGGTGGG GATGAAATCG GTTGATGCTC CTGACATAAG CCGGGTGAAA GGCGATCATA GCGTG
Details for AnphA.00817.a.A1.GE26660
| HARVESTED ON: | 5/15/2009 |
| SEQUENCED ON: | 6/18/2009 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRTGMLME GKKGVIIGVA NDKSLAWGIA KAVCAQGAEV ALTYLSETFK KRVDPLAESL GVKLTVPCDV SDAESVDNMF KVLAEEWGSL DFVVHAVAFS DKNELKGRYV DTSLGNFLTS MHISCYSFTY IASKAEPLMT NGGSILTLSY YGAEKVVPHY NVMGVCKAAL EASVKYLAVD LGKQQIRVNA ISAGPVRTLA SSGISDFHYI LTWNKYNSPL RRNTTLDDVG GAALYLLSDL GRGTTGETVH VDCGYHVVGM KSVDAPDISR VKGDHSV
Validated NT Sequence
ttattacacg ctatgatcgc ctttcacccg gcttatgtca ggagcatcaa ccgatttcat ccccaccaca tggtacccac aatccacatg cacagtctca ccagttgtcc ctctacccaa atcactcagc agatataacg cagccccccc gacatcatca agagtagtat tgcgcctcaa cggcgaatta tacttattcc acgtcagtat ataatgaaaa tcactaattc cagaagacgc caaagttcta acaggccccg cagaaatagc attaactctt atctgctgct tacccaaatc aaccgccaaa tatttaacac ttgcctcgag ggccgccttg cacacgccca tcacattgta atggggcact accttctcag caccgtaata agagagagtc aaaatactgc caccattggt catcagtggc tcagctttac tcgcaatgta agtaaaagaa taacacgata tatgcatcga cgtcaggaag ttacccagag aagtatcaac ataccttccc ttgagctcgt tcttatcgga aaatgcaacc gcgtggacca caaaatccag actaccccac tcctcagcaa gaactttgaa catattgtct acagattccg catctgatac atcacacgga accgtcaact taacacctag agactccgca aggggatcta cccttttctt aaaagtctcc gaaagatatg taagagcaac ctcggcaccc tgtgcacaca cagctttcgc gatgccccac gccaaggact tatcattagc tacaccaatg atgactccct tttttccttc catgagcatg ccggtacgca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRTGMLME GKKGVIIGVA NDKSLAWGIA KAVCAQGAEV ALTYLSETFK KRVDPLAESL GVKLTVPCDV SDAESVDNMF KVLAEEWGSL DFVVHAVAFS DKNELKGRYV DTSLGNFLTS MHISCYSFTY IASKAEPLMT NGGSILTLSY YGAEKVVPHY NVMGVCKAAL EASVKYLAVD LGKQQIRVNA ISAGPVRTLA SSGISDFHYI LTWNKYNSPL RRNTTLDDVG GAALYLLSDL GRGTTGETVH VDCGYHVVGM KSVDAPDISR VKGDHSV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgtacc ggcatgctca tggaaggaaa aaagggagtc atcattggtg tagctaatga taagtccttg gcgtggggca tcgcgaaagc tgtgtgtgca cagggtgccg aggttgctct tacatatctt tcggagactt ttaagaaaag ggtagatccc cttgcggagt ctctaggtgt taagttgacg gttccgtgtg atgtatcaga tgcggaatct gtagacaata tgttcaaagt tcttgctgag gagtggggta gtctggattt tgtggtccac gcggttgcat tttccgataa gaacgagctc aagggaaggt atgttgatac ttctctgggt aacttcctga cgtcgatgca tatatcgtgt tattctttta cttacattgc gagtaaagct gagccactga tgaccaatgg tggcagtatt ttgactctct cttattacgg tgctgagaag gtagtgcccc attacaatgt gatgggcgtg tgcaaggcgg ccctcgaggc aagtgttaaa tatttggcgg ttgatttggg taagcagcag ataagagtta atgctatttc tgcggggcct gttagaactt tggcgtcttc tggaattagt gattttcatt atatactgac gtggaataag tataattcgc cgttgaggcg caatactact cttgatgatg tcgggggggc tgcgttatat ctgctgagtg atttgggtag agggacaact ggtgagactg tgcatgtgga ttgtgggtac catgtggtgg ggatgaaatc ggttgatgct cctgacataa gccgggtgaa aggcgatcat agcgtgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for AnphA.00817.a.A1.PS00247
| PURIFICATION DATe: | 7/14/2009 |
| CONCENTRATION: | 23mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRTGMLME GKKGVIIGVA NDKSLAWGIA KAVCAQGAEV ALTYLSETFK KRVDPLAESL GVKLTVPCDV SDAESVDNMF KVLAEEWGSL DFVVHAVAFS DKNELKGRYV DTSLGNFLTS MHISCYSFTY IASKAEPLMT NGGSILTLSY YGAEKVVPHY NVMGVCKAAL EASVKYLAVD LGKQQIRVNA ISAGPVRTLA SSGISDFHYI LTWNKYNSPL RRNTTLDDVG GAALYLLSDL GRGTTGETVH VDCGYHVVGM KSVDAPDISR VKGDHSV
Validated NT Sequence
ttattacacg ctatgatcgc ctttcacccg gcttatgtca ggagcatcaa ccgatttcat ccccaccaca tggtacccac aatccacatg cacagtctca ccagttgtcc ctctacccaa atcactcagc agatataacg cagccccccc gacatcatca agagtagtat tgcgcctcaa cggcgaatta tacttattcc acgtcagtat ataatgaaaa tcactaattc cagaagacgc caaagttcta acaggccccg cagaaatagc attaactctt atctgctgct tacccaaatc aaccgccaaa tatttaacac ttgcctcgag ggccgccttg cacacgccca tcacattgta atggggcact accttctcag caccgtaata agagagagtc aaaatactgc caccattggt catcagtggc tcagctttac tcgcaatgta agtaaaagaa taacacgata tatgcatcga cgtcaggaag ttacccagag aagtatcaac ataccttccc ttgagctcgt tcttatcgga aaatgcaacc gcgtggacca caaaatccag actaccccac tcctcagcaa gaactttgaa catattgtct acagattccg catctgatac atcacacgga accgtcaact taacacctag agactccgca aggggatcta cccttttctt aaaagtctcc gaaagatatg taagagcaac ctcggcaccc tgtgcacaca cagctttcgc gatgccccac gccaaggact tatcattagc tacaccaatg atgactccct tttttccttc catgagcatg ccggtacgca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRTGMLME GKKGVIIGVA NDKSLAWGIA KAVCAQGAEV ALTYLSETFK KRVDPLAESL GVKLTVPCDV SDAESVDNMF KVLAEEWGSL DFVVHAVAFS DKNELKGRYV DTSLGNFLTS MHISCYSFTY IASKAEPLMT NGGSILTLSY YGAEKVVPHY NVMGVCKAAL EASVKYLAVD LGKQQIRVNA ISAGPVRTLA SSGISDFHYI LTWNKYNSPL RRNTTLDDVG GAALYLLSDL GRGTTGETVH VDCGYHVVGM KSVDAPDISR VKGDHSV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCGTACC GGCATGCTCA TGGAAGGAAA AAAGGGAGTC ATCATTGGTG TAGCTAATGA TAAGTCCTTG GCGTGGGGCA TCGCGAAAGC TGTGTGTGCA CAGGGTGCCG AGGTTGCTCT TACATATCTT TCGGAGACTT TTAAGAAAAG GGTAGATCCC CTTGCGGAGT CTCTAGGTGT TAAGTTGACG GTTCCGTGTG ATGTATCAGA TGCGGAATCT GTAGACAATA TGTTCAAAGT TCTTGCTGAG GAGTGGGGTA GTCTGGATTT TGTGGTCCAC GCGGTTGCAT TTTCCGATAA GAACGAGCTC AAGGGAAGGT ATGTTGATAC TTCTCTGGGT AACTTCCTGA CGTCGATGCA TATATCGTGT TATTCTTTTA CTTACATTGC GAGTAAAGCT GAGCCACTGA TGACCAATGG TGGCAGTATT TTGACTCTCT CTTATTACGG TGCTGAGAAG GTAGTGCCCC ATTACAATGT GATGGGCGTG TGCAAGGCGG CCCTCGAGGC AAGTGTTAAA TATTTGGCGG TTGATTTGGG TAAGCAGCAG ATAAGAGTTA ATGCTATTTC TGCGGGGCCT GTTAGAACTT TGGCGTCTTC TGGAATTAGT GATTTTCATT ATATACTGAC GTGGAATAAG TATAATTCGC CGTTGAGGCG CAATACTACT CTTGATGATG TCGGGGGGGC TGCGTTATAT CTGCTGAGTG ATTTGGGTAG AGGGACAACT GGTGAGACTG TGCATGTGGA TTGTGGGTAC CATGTGGTGG GGATGAAATC GGTTGATGCT CCTGACATAA GCCGGGTGAA AGGCGATCAT AGCGTGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for AnphA.00817.a.A1.PS00248
| PURIFICATION DATe: | 7/14/2009 |
| CONCENTRATION: | 12mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRTGMLME GKKGVIIGVA NDKSLAWGIA KAVCAQGAEV ALTYLSETFK KRVDPLAESL GVKLTVPCDV SDAESVDNMF KVLAEEWGSL DFVVHAVAFS DKNELKGRYV DTSLGNFLTS MHISCYSFTY IASKAEPLMT NGGSILTLSY YGAEKVVPHY NVMGVCKAAL EASVKYLAVD LGKQQIRVNA ISAGPVRTLA SSGISDFHYI LTWNKYNSPL RRNTTLDDVG GAALYLLSDL GRGTTGETVH VDCGYHVVGM KSVDAPDISR VKGDHSV
Validated NT Sequence
ttattacacg ctatgatcgc ctttcacccg gcttatgtca ggagcatcaa ccgatttcat ccccaccaca tggtacccac aatccacatg cacagtctca ccagttgtcc ctctacccaa atcactcagc agatataacg cagccccccc gacatcatca agagtagtat tgcgcctcaa cggcgaatta tacttattcc acgtcagtat ataatgaaaa tcactaattc cagaagacgc caaagttcta acaggccccg cagaaatagc attaactctt atctgctgct tacccaaatc aaccgccaaa tatttaacac ttgcctcgag ggccgccttg cacacgccca tcacattgta atggggcact accttctcag caccgtaata agagagagtc aaaatactgc caccattggt catcagtggc tcagctttac tcgcaatgta agtaaaagaa taacacgata tatgcatcga cgtcaggaag ttacccagag aagtatcaac ataccttccc ttgagctcgt tcttatcgga aaatgcaacc gcgtggacca caaaatccag actaccccac tcctcagcaa gaactttgaa catattgtct acagattccg catctgatac atcacacgga accgtcaact taacacctag agactccgca aggggatcta cccttttctt aaaagtctcc gaaagatatg taagagcaac ctcggcaccc tgtgcacaca cagctttcgc gatgccccac gccaaggact tatcattagc tacaccaatg atgactccct tttttccttc catgagcatg ccggtacgca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRTGMLME GKKGVIIGVA NDKSLAWGIA KAVCAQGAEV ALTYLSETFK KRVDPLAESL GVKLTVPCDV SDAESVDNMF KVLAEEWGSL DFVVHAVAFS DKNELKGRYV DTSLGNFLTS MHISCYSFTY IASKAEPLMT NGGSILTLSY YGAEKVVPHY NVMGVCKAAL EASVKYLAVD LGKQQIRVNA ISAGPVRTLA SSGISDFHYI LTWNKYNSPL RRNTTLDDVG GAALYLLSDL GRGTTGETVH VDCGYHVVGM KSVDAPDISR VKGDHSV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCGTACC GGCATGCTCA TGGAAGGAAA AAAGGGAGTC ATCATTGGTG TAGCTAATGA TAAGTCCTTG GCGTGGGGCA TCGCGAAAGC TGTGTGTGCA CAGGGTGCCG AGGTTGCTCT TACATATCTT TCGGAGACTT TTAAGAAAAG GGTAGATCCC CTTGCGGAGT CTCTAGGTGT TAAGTTGACG GTTCCGTGTG ATGTATCAGA TGCGGAATCT GTAGACAATA TGTTCAAAGT TCTTGCTGAG GAGTGGGGTA GTCTGGATTT TGTGGTCCAC GCGGTTGCAT TTTCCGATAA GAACGAGCTC AAGGGAAGGT ATGTTGATAC TTCTCTGGGT AACTTCCTGA CGTCGATGCA TATATCGTGT TATTCTTTTA CTTACATTGC GAGTAAAGCT GAGCCACTGA TGACCAATGG TGGCAGTATT TTGACTCTCT CTTATTACGG TGCTGAGAAG GTAGTGCCCC ATTACAATGT GATGGGCGTG TGCAAGGCGG CCCTCGAGGC AAGTGTTAAA TATTTGGCGG TTGATTTGGG TAAGCAGCAG ATAAGAGTTA ATGCTATTTC TGCGGGGCCT GTTAGAACTT TGGCGTCTTC TGGAATTAGT GATTTTCATT ATATACTGAC GTGGAATAAG TATAATTCGC CGTTGAGGCG CAATACTACT CTTGATGATG TCGGGGGGGC TGCGTTATAT CTGCTGAGTG ATTTGGGTAG AGGGACAACT GGTGAGACTG TGCATGTGGA TTGTGGGTAC CATGTGGTGG GGATGAAATC GGTTGATGCT CCTGACATAA GCCGGGTGAA AGGCGATCAT AGCGTGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA