AnphA.00715.a

putative tRNA-dihydrouridine synthase

CENTER ID: AnphA.00715.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00715.a.B1.GE26916 Full length( AnphA.00715.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.624
RefSeq: YP_505166.1
UniProt: Q2GKD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRQFISENPV ILAPMSGVTD LPFRTTVRKL GAGLLISEMV ASRAMILKTR QSMQKSEVCP NTSVQLAGCT PEIMAEAAKI NEDLGAKSID LNFGCPAKKV VDGYAGSALM KDEVIATKIM ESVVKAVKVP VTVKMRMGWD TTNLNAPTLA KVAEDVGIQM ITIHGRTRSQ MFSGSADWKF VKRVKECVKI PVIVNGDIRT LDDIVVALNE SGADGVMIGR GAYGKPWLIK QALSFMKNSE ILPEPTKSEK LAIILDHYDY ALDYYGKDLG VKILRKHLSW YSSSYSGASY FRAQVNTMSD PVEVREKLTE FFQTS
NT Sequence
ATGAGGCAAT TCATCTCAGA AAACCCAGTT ATACTTGCCC CTATGTCCGG CGTTACCGAC TTGCCTTTTC GAACAACAGT ACGAAAGCTG GGAGCTGGGC TTTTGATATC TGAAATGGTG GCCAGTAGGG CAATGATCTT AAAAACTCGG CAAAGTATGC AAAAATCGGA AGTGTGTCCT AATACTTCTG TGCAATTGGC TGGTTGTACT CCTGAAATTA TGGCAGAAGC GGCTAAGATT AATGAAGATC TAGGTGCGAA ATCTATAGAT CTAAATTTTG GATGTCCTGC CAAAAAAGTT GTGGATGGCT ATGCGGGCTC TGCATTAATG AAGGATGAAG TTATCGCGAC GAAAATAATG GAAAGCGTGG TCAAGGCGGT AAAAGTTCCT GTCACAGTAA AAATGCGCAT GGGCTGGGAT ACTACAAATC TGAATGCACC TACTTTGGCC AAAGTTGCTG AAGATGTAGG AATTCAGATG ATTACCATTC ACGGCAGAAC GCGTTCACAG ATGTTTAGTG GCTCTGCAGA CTGGAAGTTC GTCAAGAGAG TAAAGGAGTG TGTAAAAATA CCAGTAATAG TAAACGGGGA TATTAGAACC TTGGATGATA TCGTAGTGGC GCTCAACGAA TCCGGGGCAG ATGGAGTGAT GATTGGCAGA GGAGCCTACG GTAAACCATG GCTAATAAAG CAAGCACTAA GCTTTATGAA AAACTCGGAG ATATTACCAG AGCCCACAAA AAGTGAAAAG TTGGCAATTA TATTGGATCA CTACGATTAT GCACTAGACT ACTATGGAAA AGATCTAGGA GTAAAAATCT TGAGGAAGCA CCTGAGTTGG TATAGCAGTT CCTATTCTGG TGCATCTTAT TTTAGAGCGC AAGTAAACAC TATGAGTGAC CCTGTAGAGG TAAGGGAAAA GCTAACAGAG TTTTTTCAAA CATCG
Details for AnphA.00715.a.B1.GE26916
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 90
Validated AA Sequence
MSXVTDLPFR TTVRKLGAGL LISEMVAXXA MILKTRQSMQ KSEVCPNTSV QLAGCTPEIM AEAAKINEDL GAKSIDLNFG CPAKKVVDGY AGSALMKDEV IATKIMESVV KAVKVPVTVK MRMGWDTTNL NAPTLAKVAE DVGIQMITIH GRTRSQMFSG SADWKFVKRV KECVKIPVIV NGDIRTLDDI VVALNESGAD GVMIGRGAYG KPWLIKQALS FMKNSEILPE PTKSEKLAII LDHYDYALDY YGKDLGVKIL RKHLSWYSSS YSGASYFRAQ VNTMSDPVEV R
Validated NT Sequence
tcccttacct ctacagggtc actcatagtg tttacttgcg ctctaaaata agatgcacca gaataggaac tgctatacca actcaggtgc ttcctcaaga tttttactcc tagatctttt ccatagtagt ctagtgcata atcgtagtga tccaatataa ttgccaactt ttcacttttt gtgggctctg gtaatatctc cgagtttttc ataaagctta gtgcttgctt tattagccat ggtttaccgt aggctcctct gccaatcatc actccatctg ccccggattc gttgagcgcc actacgatat catccaaggt tctaatatcc ccgtttacta ttactggtat ttttacacac tcctttactc tcttgacgaa cttccagtct gcagagccac taaacatctg tgaacgcgtt ctgccgtgaa tggtaatcat ctgaattcct acatcttcag caactttggc caaagtaggt gcattcagat ttgtagtatc ccagcccatg cgcattttta ctgtgacagg aacttttacc gccttgacca cgctttccat tattttcgtc gcgataactt catccttcat taatgcagag cccgcatagc catccacaac ttttttggca ggacatccaa aatttagatc tatagatttc gcacctagat cttcattaat cttagccgct tctgccataa tttcaggagt acaaccagcc aattgcacag aagtattagg acacacttcc gatttttgca tactttgccg agtttttaag atcattgcnn nnnnggccac catttcagat atcaaaagcc cagctcccag ctttcgtact gttgttcgaa aaggcaagtc ggtaacnncg gacatagggg caagtataac tgggttttct gagatnaa
Expected Protein Sequence
MAHHHHHHMR QFISENPVIL APMSGVTDLP FRTTVRKLGA GLLISEMVAS RAMILKTRQS MQKSEVCPNT SVQLAGCTPE IMAEAAKINE DLGAKSIDLN FGCPAKKVVD GYAGSALMKD EVIATKIMES VVKAVKVPVT VKMRMGWDTT NLNAPTLAKV AEDVGIQMIT IHGRTRSQMF SGSADWKFVK RVKECVKIPV IVNGDIRTLD DIVVALNESG ADGVMIGRGA YGKPWLIKQA LSFMKNSEIL PEPTKSEKLA IILDHYDYAL DYYGKDLGVK ILRKHLSWYS SSYSGASYFR AQVNTMSDPV EVREKLTEFF QTS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaggc aattcatctc agaaaaccca gttatacttg cccctatgtc cggcgttacc gacttgcctt ttcgaacaac agtacgaaag ctgggagctg ggcttttgat atctgaaatg gtggccagta gggcaatgat cttaaaaact cggcaaagta tgcaaaaatc ggaagtgtgt cctaatactt ctgtgcaatt ggctggttgt actcctgaaa ttatggcaga agcggctaag attaatgaag atctaggtgc gaaatctata gatctaaatt ttggatgtcc tgccaaaaaa gttgtggatg gctatgcggg ctctgcatta atgaaggatg aagttatcgc gacgaaaata atggaaagcg tggtcaaggc ggtaaaagtt cctgtcacag taaaaatgcg catgggctgg gatactacaa atctgaatgc acctactttg gccaaagttg ctgaagatgt aggaattcag atgattacca ttcacggcag aacgcgttca cagatgttta gtggctctgc agactggaag ttcgtcaaga gagtaaagga gtgtgtaaaa ataccagtaa tagtaaacgg ggatattaga accttggatg atatcgtagt ggcgctcaac gaatccgggg cagatggagt gatgattggc agaggagcct acggtaaacc atggctaata aagcaagcac taagctttat gaaaaactcg gagatattac cagagcccac aaaaagtgaa aagttggcaa ttatattgga tcactacgat tatgcactag actactatgg aaaagatcta ggagtaaaaa tcttgaggaa gcacctgagt tggtatagca gttcctattc tggtgcatct tattttagag cgcaagtaaa cactatgagt gaccctgtag aggtaaggga aaagctaaca gagttttttc aaacatcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat