AnphA.00677.a
phosphoribosylglycinamide formyltransferase
| CENTER ID: | AnphA.00677.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.00677.a.A1.GE26653 | Full length( AnphA.00677.a ) | 1 | 211 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.00677.a.A1.PS00239 | Full length( AnphA.00677.a ) | 1 | 211 |
Structures
3KCQ
DEPOSITED: 10/21/2009
DETERMINATION: XRay
CLONE: AnphA.00677.a.A1.GE26653
PROTEIN: AnphA.00677.a.A1.PS00239
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129694 |
| PATRIC ID: | fig|212042.8.peg.244 |
| RefSeq: | YP_504847.1 |
| UniProt: | Q2GLA4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKELRVGVL ISGRGSNLEA LAKAFSTEES SVVISCVISN NAEARGLLIA QSYGIPTFVV KRKPLDIEHI STVLREHDVD LVCLAGFMSI LPEKFVTDWH HKIINIHPSL LPSFKGLNAQ EQAYKAGVKI AGCTLHYVYQ ELDAGPIIMQ AAVPVLREDT AESLASRILA AEHVCYPKGV KLIAQDKIKL CDDGTVQCTG EDELFLFQEN F
NT Sequence
GTGAAGAAGG AACTACGCGT AGGTGTATTG ATTTCTGGTA GGGGTTCTAA TCTTGAAGCG TTAGCTAAGG CGTTTTCGAC TGAAGAGTCC TCTGTTGTGA TTTCATGTGT TATTTCTAAT AATGCTGAAG CACGCGGGCT CTTGATCGCT CAGAGTTACG GAATTCCTAC CTTTGTAGTT AAGCGGAAAC CGCTGGATAT AGAGCATATC AGTACGGTAC TAAGGGAGCA TGATGTAGAT CTAGTGTGCC TTGCAGGATT CATGAGCATA CTGCCTGAAA AGTTTGTCAC AGATTGGCAT CACAAGATAA TTAACATTCA CCCTTCACTT CTCCCATCTT TTAAGGGATT AAATGCTCAG GAACAAGCCT ATAAAGCAGG TGTTAAAATA GCGGGGTGCA CCCTACATTA TGTATATCAA GAGCTTGATG CCGGTCCTAT AATAATGCAG GCTGCAGTGC CTGTGCTTAG AGAAGACACT GCTGAGAGTT TAGCTAGTCG TATTCTAGCT GCTGAGCACG TATGTTATCC CAAGGGTGTG AAGCTCATTG CTCAGGATAA AATCAAGCTT TGCGATGATG GCACTGTGCA ATGTACGGGC GAGGATGAGC TCTTCTTATT TCAGGAGAAC TTT
Details for AnphA.00677.a.A1.GE26653
| HARVESTED ON: | 5/15/2009 |
| SEQUENCED ON: | 6/18/2009 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKELRVG VLISGRGSNL EALAKAFSTE ESSVVISCVI SNNAEARGLL IAQSYGIPTF VVKRKPLDIE HISTVLREHD VDLVCLAGFM SILPEKFVTD WHHKIINIHP SLLPSFKGLN AQEQAYKAGV KIAGCTLHYV YQELDAGPII MQAAVPVLRE DTAESLASRI LAAEHVCYPK GVKLIAQDKI KLCDDGTVQC TGEDELFLFQ E
Validated NT Sequence
tctcctgaaa taagaagagc tcatcctcgc ccgtacattg cacagtgcca tcatcgcaaa gcttgatttt atcctgagca atgagcttca cacccttggg ataacatacg tgctcagcag ctagaatacg actagctaaa ctctcagcag tgtcttctct aagcacaggc actgcagcct gcattattat aggaccggca tcaagctctt gatatacata atgtagggtg caccccgcta ttttaacacc tgctttatag gcttgttcct gagcatttaa tcccttaaaa gatgggagaa gtgaagggtg aatgttaatt atcttgtgat gccaatctgt gacaaacttt tcaggcagta tgctcatgaa tcctgcaagg cacactagat ctacatcatg ctcccttagt accgtactga tatgctctat atccagcggt ttccgcttaa ctacaaaggt aggaattccg taactctgag cgatcaagag cccgcgtgct tcagcattat tagaaataac acatgaaatc acaacagagg actcttcagt cgaaaacgcc ttagctaacg cttcaagatt agaaccccta ccagaaatca atacacctac gcgtagttcc ttcttcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKELRVG VLISGRGSNL EALAKAFSTE ESSVVISCVI SNNAEARGLL IAQSYGIPTF VVKRKPLDIE HISTVLREHD VDLVCLAGFM SILPEKFVTD WHHKIINIHP SLLPSFKGLN AQEQAYKAGV KIAGCTLHYV YQELDAGPII MQAAVPVLRE DTAESLASRI LAAEHVCYPK GVKLIAQDKI KLCDDGTVQC TGEDELFLFQ ENF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagaag gaactacgcg taggtgtatt gatttctggt aggggttcta atcttgaagc gttagctaag gcgttttcga ctgaagagtc ctctgttgtg atttcatgtg ttatttctaa taatgctgaa gcacgcgggc tcttgatcgc tcagagttac ggaattccta cctttgtagt taagcggaaa ccgctggata tagagcatat cagtacggta ctaagggagc atgatgtaga tctagtgtgc cttgcaggat tcatgagcat actgcctgaa aagtttgtca cagattggca tcacaagata attaacattc acccttcact tctcccatct tttaagggat taaatgctca ggaacaagcc tataaagcag gtgttaaaat agcggggtgc accctacatt atgtatatca agagcttgat gccggtccta taataatgca ggctgcagtg cctgtgctta gagaagacac tgctgagagt ttagctagtc gtattctagc tgctgagcac gtatgttatc ccaagggtgt gaagctcatt gctcaggata aaatcaagct ttgcgatgat ggcactgtgc aatgtacggg cgaggatgag ctcttcttat ttcaggagaa ctttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for AnphA.00677.a.A1.PS00239
| PURIFICATION DATe: | 7/15/2009 |
| CONCENTRATION: | 54.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKELRVG VLISGRGSNL EALAKAFSTE ESSVVISCVI SNNAEARGLL IAQSYGIPTF VVKRKPLDIE HISTVLREHD VDLVCLAGFM SILPEKFVTD WHHKIINIHP SLLPSFKGLN AQEQAYKAGV KIAGCTLHYV YQELDAGPII MQAAVPVLRE DTAESLASRI LAAEHVCYPK GVKLIAQDKI KLCDDGTVQC TGEDELFLFQ E
Validated NT Sequence
tctcctgaaa taagaagagc tcatcctcgc ccgtacattg cacagtgcca tcatcgcaaa gcttgatttt atcctgagca atgagcttca cacccttggg ataacatacg tgctcagcag ctagaatacg actagctaaa ctctcagcag tgtcttctct aagcacaggc actgcagcct gcattattat aggaccggca tcaagctctt gatatacata atgtagggtg caccccgcta ttttaacacc tgctttatag gcttgttcct gagcatttaa tcccttaaaa gatgggagaa gtgaagggtg aatgttaatt atcttgtgat gccaatctgt gacaaacttt tcaggcagta tgctcatgaa tcctgcaagg cacactagat ctacatcatg ctcccttagt accgtactga tatgctctat atccagcggt ttccgcttaa ctacaaaggt aggaattccg taactctgag cgatcaagag cccgcgtgct tcagcattat tagaaataac acatgaaatc acaacagagg actcttcagt cgaaaacgcc ttagctaacg cttcaagatt agaaccccta ccagaaatca atacacctac gcgtagttcc ttcttcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKKELRVG VLISGRGSNL EALAKAFSTE ESSVVISCVI SNNAEARGLL IAQSYGIPTF VVKRKPLDIE HISTVLREHD VDLVCLAGFM SILPEKFVTD WHHKIINIHP SLLPSFKGLN AQEQAYKAGV KIAGCTLHYV YQELDAGPII MQAAVPVLRE DTAESLASRI LAAEHVCYPK GVKLIAQDKI KLCDDGTVQC TGEDELFLFQ ENF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGAAG GAACTACGCG TAGGTGTATT GATTTCTGGT AGGGGTTCTA ATCTTGAAGC GTTAGCTAAG GCGTTTTCGA CTGAAGAGTC CTCTGTTGTG ATTTCATGTG TTATTTCTAA TAATGCTGAA GCACGCGGGC TCTTGATCGC TCAGAGTTAC GGAATTCCTA CCTTTGTAGT TAAGCGGAAA CCGCTGGATA TAGAGCATAT CAGTACGGTA CTAAGGGAGC ATGATGTAGA TCTAGTGTGC CTTGCAGGAT TCATGAGCAT ACTGCCTGAA AAGTTTGTCA CAGATTGGCA TCACAAGATA ATTAACATTC ACCCTTCACT TCTCCCATCT TTTAAGGGAT TAAATGCTCA GGAACAAGCC TATAAAGCAG GTGTTAAAAT AGCGGGGTGC ACCCTACATT ATGTATATCA AGAGCTTGAT GCCGGTCCTA TAATAATGCA GGCTGCAGTG CCTGTGCTTA GAGAAGACAC TGCTGAGAGT TTAGCTAGTC GTATTCTAGC TGCTGAGCAC GTATGTTATC CCAAGGGTGT GAAGCTCATT GCTCAGGATA AAATCAAGCT TTGCGATGAT GGCACTGTGC AATGTACGGG CGAGGATGAG CTCTTCTTAT TTCAGGAGAA CTTTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA