AnphA.00668.a

ATP-dependent protease ATP-binding subunit

CENTER ID: AnphA.00668.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00668.a.A1.GE34606 Full length( AnphA.00668.a ) 1 437
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00668.a.A1.PS01465 Full length( AnphA.00668.a ) 1 437
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_129971
PATRIC ID: fig|212042.8.peg.1150
RefSeq: YP_505627.1
UniProt: Q2GJ24
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDLSPKQIT KELDRFIVGQ TEAKRAVANA LRSRWRRNRV PQPLRDEIIP KNILMIGHTG VGKTEIARRL AKLAQAPFIK VEATKFTEIG YVGRDVDSIM RDLVDRAVLL VKEKYRKVVR KQARKSAEDI ILNCLVGADA SEETKNAFRH KLRAGEYENS EISINVKDNR KSMPPSFDVP GMPGGQVGIM NINEIVQKVL GGNKLSRTIS TTVKEAREIL LDEETEKLID EDKIVREALH IASNEGIVFL DEIDKIAART EVRGEVNREG VQRDLLPLLE GTSVSTKYGT VTTDHVLFIA SGAFHLAKPS DLLPELQGRL PIRVELQPLS RDDLVRILTE PEANLLKQYR ALLETEGVTV EFTEDGIFAI AEIASTVNRE VENIGARRLH TIMEKLMEDI SYNASENSGQ TFVIDEKHVR ERLEDISKQL DLSKFIL
NT Sequence
ATTCGTGACC TATCTCCGAA GCAGATAACA AAAGAACTGG ATAGGTTCAT AGTTGGGCAA ACTGAAGCAA AGCGTGCAGT TGCGAATGCT CTTAGGAGCC GTTGGCGTCG CAATCGCGTA CCTCAACCCT TAAGAGATGA AATCATCCCG AAAAATATAC TGATGATAGG GCACACTGGG GTTGGAAAAA CAGAAATAGC TCGTCGTTTA GCTAAACTAG CTCAAGCACC GTTTATAAAG GTGGAAGCTA CCAAATTTAC CGAAATAGGT TACGTTGGCC GTGATGTTGA TTCCATAATG CGCGACCTAG TGGACCGAGC AGTGTTATTA GTTAAGGAAA AGTATAGAAA AGTTGTCAGA AAGCAAGCAC GGAAGTCTGC TGAAGATATT ATCCTTAACT GCCTTGTAGG AGCAGATGCA TCGGAGGAAA CCAAAAACGC TTTCCGGCAT AAACTCCGCG CAGGAGAGTA CGAAAACTCT GAAATATCTA TCAATGTTAA AGATAATCGC AAGAGCATGC CCCCTTCGTT TGACGTTCCA GGAATGCCGG GCGGGCAGGT CGGCATTATG AATATCAATG AGATAGTGCA GAAGGTTCTA GGAGGGAACA AACTCTCAAG AACCATTAGT ACCACTGTAA AAGAGGCTCG CGAGATTCTC CTAGACGAAG AAACCGAGAA GCTGATTGAC GAAGATAAGA TAGTACGCGA AGCATTGCAC ATTGCTAGCA ACGAGGGAAT AGTCTTTTTA GATGAGATTG ATAAAATTGC TGCAAGAACA GAAGTACGCG GTGAAGTAAA TAGAGAGGGT GTGCAGCGTG ATCTTCTGCC ATTGCTAGAA GGCACAAGTG TAAGCACTAA GTATGGTACC GTAACTACTG ACCATGTCCT ATTTATAGCT TCCGGTGCGT TCCATCTAGC AAAGCCTTCA GACCTACTGC CAGAGCTTCA AGGTCGTTTG CCTATCAGAG TAGAGCTGCA GCCTTTAAGC AGAGATGACC TAGTGCGTAT CCTAACAGAG CCAGAGGCTA ATCTGTTGAA ACAGTATCGC GCATTGCTGG AAACCGAAGG TGTTACGGTA GAATTCACTG AGGACGGCAT ATTTGCCATT GCTGAAATAG CTTCAACTGT AAACAGAGAA GTGGAAAACA TCGGTGCGAG AAGGCTTCAT ACCATCATGG AAAAGCTAAT GGAAGATATC AGCTACAATG CTTCTGAAAA CAGTGGTCAA ACCTTCGTGA TAGACGAAAA GCATGTACGG GAAAGGCTGG AGGATATTTC GAAGCAATTA GATCTTTCCA AGTTTATACT G
Details for AnphA.00668.a.A1.GE34606
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIRDLSPKQ ITKELDRFIV GQTEAKRAVA NALRSRWRRN RVPQPLRDEI IPKNILMIGH TGVGKTEIAR RLAKLAQAPF IKVEATKFTE IGYVGRDVDS IMRDLVDRAV LLVKEKYRKV VRKQARKSAE DIILNCLVGA DASEETKNAF RHKLRAGEYE NSEISINVKD NRKSMPPSFD VPGMPGGQVG IMNINEIVQK VLGGNKLSRT ISTTVKEARE ILLDEETEKL IDEDKIVREA LHIASNEGIV FLDEIDKIAA RTEVRGEVNR EGVQRDLLPL LEGTSVSTKY GTVTTDHVLF IASGAFHLAK PSDLLPELQG RLPIRVELQP LSRDDLVRIL TEPEANLLKQ YRALLETEGV TVEFTEDGIF AIAEIASTVN REVENIGARR LHTIMEKLME DISYNASENS GQTFVIDEKH VRERLEDISK QLDLSK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattc gtgacctatc tccgaagcag ataacaaaag aactggatag gttcatagtt gggcaaactg aagcaaagcg tgcagttgcg aatgctctta ggagccgttg gcgtcgcaat cgcgtacctc aacccttaag agatgaaatc atcccgaaaa atatactgat gatagggcac actggggttg gaaaaacaga aatagctcgt cgtttagcta aactagctca agcaccgttt ataaaggtgg aagctaccaa atttaccgaa ataggttacg ttggccgtga tgttgattcc ataatgcgcg acctagtgga ccgagcagtg ttattagtta aggaaaagta tagaaaagtt gtcagaaagc aagcacggaa gtctgctgaa gatattatcc ttaactgcct tgtaggagca gatgcatcgg aggaaaccaa aaacgctttc cggcataaac tccgcgcagg agagtacgaa aactctgaaa tatctatcaa tgttaaagat aatcgcaaga gcatgccccc ttcgtttgac gttccaggaa tgccgggcgg gcaggtcggc attatgaata tcaatgagat agtgcagaag gttctaggag ggaacaaact ctcaagaacc attagtacca ctgtaaaaga ggctcgcgag attctcctag acgaagaaac cgagaagctg attgacgaag ataagatagt acgcgaagca ttgcacattg ctagcaacga gggaatagtc tttttagatg agattgataa aattgctgca agaacagaag tacgcggtga agtaaataga gagggtgtgc agcgtgatct tctgccattg ctagaaggca caagtgtaag cactaagtat ggtaccgtaa ctactgacca tgtcctattt atagcttccg gtgcgttcca tctagcaaag ccttcagacc tactgccaga gcttcaaggt cgtttgccta tcagagtaga gctgcagcct ttaagcagag atgacctagt gcgtatccta acagagccag aggctaatct gttgaaacag tatcgcgcat tgctggaaac cgaaggtgtt acggtagaat tcactgagga cggcatattt gccattgctg aaatagcttc aactgtaaac agagaagtgg aaaacatcgg tgcgagaagg cttcatacca tcatggaaaa gctaatggaa gatatcagct acaatgcttc tgaaaacagt ggtcaaacct tcgtgataga cgaaaagcat gtacgggaaa ggctggagga tatttcgaag caattagatc tttccaagt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIRDLSPKQ ITKELDRFIV GQTEAKRAVA NALRSRWRRN RVPQPLRDEI IPKNILMIGH TGVGKTEIAR RLAKLAQAPF IKVEATKFTE IGYVGRDVDS IMRDLVDRAV LLVKEKYRKV VRKQARKSAE DIILNCLVGA DASEETKNAF RHKLRAGEYE NSEISINVKD NRKSMPPSFD VPGMPGGQVG IMNINEIVQK VLGGNKLSRT ISTTVKEARE ILLDEETEKL IDEDKIVREA LHIASNEGIV FLDEIDKIAA RTEVRGEVNR EGVQRDLLPL LEGTSVSTKY GTVTTDHVLF IASGAFHLAK PSDLLPELQG RLPIRVELQP LSRDDLVRIL TEPEANLLKQ YRALLETEGV TVEFTEDGIF AIAEIASTVN REVENIGARR LHTIMEKLME DISYNASENS GQTFVIDEKH VRERLEDISK QLDLSKFIL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattcgtgac ctatctccga agcagataac aaaagaactg gataggttca tagttgggca aactgaagca aagcgtgcag ttgcgaatgc tcttaggagc cgttggcgtc gcaatcgcgt acctcaaccc ttaagagatg aaatcatccc gaaaaatata ctgatgatag ggcacactgg ggttggaaaa acagaaatag ctcgtcgttt agctaaacta gctcaagcac cgtttataaa ggtggaagct accaaattta ccgaaatagg ttacgttggc cgtgatgttg attccataat gcgcgaccta gtggaccgag cagtgttatt agttaaggaa aagtatagaa aagttgtcag aaagcaagca cggaagtctg ctgaagatat tatccttaac tgccttgtag gagcagatgc atcggaggaa accaaaaacg ctttccggca taaactccgc gcaggagagt acgaaaactc tgaaatatct atcaatgtta aagataatcg caagagcatg cccccttcgt ttgacgttcc aggaatgccg ggcgggcagg tcggcattat gaatatcaat gagatagtgc agaaggttct aggagggaac aaactctcaa gaaccattag taccactgta aaagaggctc gcgagattct cctagacgaa gaaaccgaga agctgattga cgaagataag atagtacgcg aagcattgca cattgctagc aacgagggaa tagtcttttt agatgagatt gataaaattg ctgcaagaac agaagtacgc ggtgaagtaa atagagaggg tgtgcagcgt gatcttctgc cattgctaga aggcacaagt gtaagcacta agtatggtac cgtaactact gaccatgtcc tatttatagc ttccggtgcg ttccatctag caaagccttc agacctactg ccagagcttc aaggtcgttt gcctatcaga gtagagctgc agcctttaag cagagatgac ctagtgcgta tcctaacaga gccagaggct aatctgttga aacagtatcg cgcattgctg gaaaccgaag gtgttacggt agaattcact gaggacggca tatttgccat tgctgaaata gcttcaactg taaacagaga agtggaaaac atcggtgcga gaaggcttca taccatcatg gaaaagctaa tggaagatat cagctacaat gcttctgaaa acagtggtca aaccttcgtg atagacgaaa agcatgtacg ggaaaggctg gaggatattt cgaagcaatt agatctttcc aagtttatac tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for AnphA.00668.a.A1.PS01465
PURIFICATION DATe: 4/18/2012
CONCENTRATION: 35.8mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIRDLSPKQ ITKELDRFIV GQTEAKRAVA NALRSRWRRN RVPQPLRDEI IPKNILMIGH TGVGKTEIAR RLAKLAQAPF IKVEATKFTE IGYVGRDVDS IMRDLVDRAV LLVKEKYRKV VRKQARKSAE DIILNCLVGA DASEETKNAF RHKLRAGEYE NSEISINVKD NRKSMPPSFD VPGMPGGQVG IMNINEIVQK VLGGNKLSRT ISTTVKEARE ILLDEETEKL IDEDKIVREA LHIASNEGIV FLDEIDKIAA RTEVRGEVNR EGVQRDLLPL LEGTSVSTKY GTVTTDHVLF IASGAFHLAK PSDLLPELQG RLPIRVELQP LSRDDLVRIL TEPEANLLKQ YRALLETEGV TVEFTEDGIF AIAEIASTVN REVENIGARR LHTIMEKLME DISYNASENS GQTFVIDEKH VRERLEDISK QLDLSK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattc gtgacctatc tccgaagcag ataacaaaag aactggatag gttcatagtt gggcaaactg aagcaaagcg tgcagttgcg aatgctctta ggagccgttg gcgtcgcaat cgcgtacctc aacccttaag agatgaaatc atcccgaaaa atatactgat gatagggcac actggggttg gaaaaacaga aatagctcgt cgtttagcta aactagctca agcaccgttt ataaaggtgg aagctaccaa atttaccgaa ataggttacg ttggccgtga tgttgattcc ataatgcgcg acctagtgga ccgagcagtg ttattagtta aggaaaagta tagaaaagtt gtcagaaagc aagcacggaa gtctgctgaa gatattatcc ttaactgcct tgtaggagca gatgcatcgg aggaaaccaa aaacgctttc cggcataaac tccgcgcagg agagtacgaa aactctgaaa tatctatcaa tgttaaagat aatcgcaaga gcatgccccc ttcgtttgac gttccaggaa tgccgggcgg gcaggtcggc attatgaata tcaatgagat agtgcagaag gttctaggag ggaacaaact ctcaagaacc attagtacca ctgtaaaaga ggctcgcgag attctcctag acgaagaaac cgagaagctg attgacgaag ataagatagt acgcgaagca ttgcacattg ctagcaacga gggaatagtc tttttagatg agattgataa aattgctgca agaacagaag tacgcggtga agtaaataga gagggtgtgc agcgtgatct tctgccattg ctagaaggca caagtgtaag cactaagtat ggtaccgtaa ctactgacca tgtcctattt atagcttccg gtgcgttcca tctagcaaag ccttcagacc tactgccaga gcttcaaggt cgtttgccta tcagagtaga gctgcagcct ttaagcagag atgacctagt gcgtatccta acagagccag aggctaatct gttgaaacag tatcgcgcat tgctggaaac cgaaggtgtt acggtagaat tcactgagga cggcatattt gccattgctg aaatagcttc aactgtaaac agagaagtgg aaaacatcgg tgcgagaagg cttcatacca tcatggaaaa gctaatggaa gatatcagct acaatgcttc tgaaaacagt ggtcaaacct tcgtgataga cgaaaagcat gtacgggaaa ggctggagga tatttcgaag caattagatc tttccaagt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIRDLSPKQ ITKELDRFIV GQTEAKRAVA NALRSRWRRN RVPQPLRDEI IPKNILMIGH TGVGKTEIAR RLAKLAQAPF IKVEATKFTE IGYVGRDVDS IMRDLVDRAV LLVKEKYRKV VRKQARKSAE DIILNCLVGA DASEETKNAF RHKLRAGEYE NSEISINVKD NRKSMPPSFD VPGMPGGQVG IMNINEIVQK VLGGNKLSRT ISTTVKEARE ILLDEETEKL IDEDKIVREA LHIASNEGIV FLDEIDKIAA RTEVRGEVNR EGVQRDLLPL LEGTSVSTKY GTVTTDHVLF IASGAFHLAK PSDLLPELQG RLPIRVELQP LSRDDLVRIL TEPEANLLKQ YRALLETEGV TVEFTEDGIF AIAEIASTVN REVENIGARR LHTIMEKLME DISYNASENS GQTFVIDEKH VRERLEDISK QLDLSKFIL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTCGTGAC CTATCTCCGA AGCAGATAAC AAAAGAACTG GATAGGTTCA TAGTTGGGCA AACTGAAGCA AAGCGTGCAG TTGCGAATGC TCTTAGGAGC CGTTGGCGTC GCAATCGCGT ACCTCAACCC TTAAGAGATG AAATCATCCC GAAAAATATA CTGATGATAG GGCACACTGG GGTTGGAAAA ACAGAAATAG CTCGTCGTTT AGCTAAACTA GCTCAAGCAC CGTTTATAAA GGTGGAAGCT ACCAAATTTA CCGAAATAGG TTACGTTGGC CGTGATGTTG ATTCCATAAT GCGCGACCTA GTGGACCGAG CAGTGTTATT AGTTAAGGAA AAGTATAGAA AAGTTGTCAG AAAGCAAGCA CGGAAGTCTG CTGAAGATAT TATCCTTAAC TGCCTTGTAG GAGCAGATGC ATCGGAGGAA ACCAAAAACG CTTTCCGGCA TAAACTCCGC GCAGGAGAGT ACGAAAACTC TGAAATATCT ATCAATGTTA AAGATAATCG CAAGAGCATG CCCCCTTCGT TTGACGTTCC AGGAATGCCG GGCGGGCAGG TCGGCATTAT GAATATCAAT GAGATAGTGC AGAAGGTTCT AGGAGGGAAC AAACTCTCAA GAACCATTAG TACCACTGTA AAAGAGGCTC GCGAGATTCT CCTAGACGAA GAAACCGAGA AGCTGATTGA CGAAGATAAG ATAGTACGCG AAGCATTGCA CATTGCTAGC AACGAGGGAA TAGTCTTTTT AGATGAGATT GATAAAATTG CTGCAAGAAC AGAAGTACGC GGTGAAGTAA ATAGAGAGGG TGTGCAGCGT GATCTTCTGC CATTGCTAGA AGGCACAAGT GTAAGCACTA AGTATGGTAC CGTAACTACT GACCATGTCC TATTTATAGC TTCCGGTGCG TTCCATCTAG CAAAGCCTTC AGACCTACTG CCAGAGCTTC AAGGTCGTTT GCCTATCAGA GTAGAGCTGC AGCCTTTAAG CAGAGATGAC CTAGTGCGTA TCCTAACAGA GCCAGAGGCT AATCTGTTGA AACAGTATCG CGCATTGCTG GAAACCGAAG GTGTTACGGT AGAATTCACT GAGGACGGCA TATTTGCCAT TGCTGAAATA GCTTCAACTG TAAACAGAGA AGTGGAAAAC ATCGGTGCGA GAAGGCTTCA TACCATCATG GAAAAGCTAA TGGAAGATAT CAGCTACAAT GCTTCTGAAA ACAGTGGTCA AACCTTCGTG ATAGACGAAA AGCATGTACG GGAAAGGCTG GAGGATATTT CGAAGCAATT AGATCTTTCC AAGTTTATAC TGAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA