SSGCID: Target Page

AnphA.00588.a

signal recognition particle-docking protein FtsY

CENTER ID:	AnphA.00588.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00588.a.A1.GE26677	Full length( AnphA.00588.a )		1	305

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1318
RefSeq:	YP_505757.1
UniProt:	Q2GIP4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSNESFWQS LKRGLSKTSA RLSEGIRNIF GGKADVDQST LKALRDLLIS TDMGPDVADF FTQKISTLKF TDSVAEEVRL KLASEIENFL QPRSAPITIT HTPHIILLCG VNGSGKTTTV AKLAHKFMKK NMSVIIGACD TFRAAAVEQL NVWAERISCP IVTGTIGADA ASVAYKTVER AVKEPFDVVI IDTAGRLHNH KNLMEELAKI CRVIRRHDEK APHDIILVLD ATIGQNTFTQ VEMFSSFVDV GGLILTKLDG TAKGGVVLRV SQKYNLMIHA IGTGEGIEDL EDFSATNFAK SLLEI

NT Sequence

ATGTCGTCAA ATGAGAGTTT CTGGCAAAGC CTGAAGAGAG GGTTGTCCAA AACTTCTGCT AGGTTAAGTG AAGGAATTAG AAACATATTT GGTGGAAAAG CCGATGTTGA TCAAAGCACT TTAAAAGCCT TACGTGATCT ACTTATCTCT ACCGATATGG GGCCTGACGT AGCTGATTTT TTTACTCAAA AAATTTCCAC CCTGAAATTT ACTGATTCAG TTGCAGAAGA AGTACGCCTA AAACTCGCTT CTGAGATTGA GAATTTTTTG CAACCAAGAT CTGCCCCGAT TACCATAACT CATACACCTC ATATTATTCT TCTATGTGGA GTAAATGGCA GCGGTAAAAC AACTACTGTT GCGAAATTAG CGCATAAGTT TATGAAGAAA AATATGTCTG TCATAATAGG TGCGTGTGAC ACTTTCCGCG CTGCAGCCGT GGAGCAACTT AACGTTTGGG CTGAAAGAAT ATCTTGCCCC ATAGTAACGG GCACTATTGG TGCTGATGCA GCAAGTGTGG CTTACAAGAC GGTTGAAAGA GCTGTAAAAG AGCCTTTTGA TGTTGTGATT ATAGACACGG CCGGTAGGCT GCATAATCAT AAAAACTTAA TGGAAGAGTT GGCGAAGATA TGTCGTGTTA TTCGCCGCCA TGATGAGAAA GCCCCCCATG ATATTATACT GGTATTAGAC GCTACTATAG GGCAAAACAC TTTCACTCAG GTAGAAATGT TTTCCAGTTT TGTGGATGTA GGCGGTCTAA TTTTGACTAA GTTGGATGGC ACAGCTAAAG GTGGTGTAGT TTTGCGTGTA TCACAGAAAT ACAACTTAAT GATACATGCT ATAGGAACTG GCGAGGGAAT AGAAGATTTG GAAGATTTTT CTGCTACTAA TTTTGCAAAG AGTTTACTGG AAATA

Details for AnphA.00588.a.A1.GE26677

HARVESTED ON:	5/15/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	86

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSSNESFWQ SLKRGLSKTS ARLSEGIRNI FGGKADVDQS TLKALRDLLI STDMGPDVAD FFTQKISTLK FTDSVAEEVR LKLASEIENF LQPRSAPITI THTPHIILLC GVNGSGKTTT VAKLAHKFMK KNMSVIIGAC DTFRAAAVEQ LNVWAERISC PIVTGTIGAD AASVAYKTVE RAVKEPFDVV IIDTAGRLHN HKNLMEELAK ICRVIRRHDE KAPHDIILVL DATIGQNTFT XVEMFSSFVD VXGLILTXLD GTA

Validated NT Sequence

ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtcaaat gagagtttct ggcaaagcct gaagagaggg ttgtccaaaa cttctgctag gttaagtgaa ggaattagaa acatatttgg tggaaaggcc gatgttgatc aaagcacttt aaaagcctta cgtgatctac ttatctctac cgatatgggg cctgacgtag ctgatttttt tactcaaaaa atttccaccc tgaaatttac tgattcagtt gcagaagaag tacgcctaaa actcgcttct gagattgaga attttttgca accaagatct gccccgatta ccataactca tacacctcat attattcttc tatgtggagt aaatggcagc ggtaaaacaa ctactgttgc gaaattagcg cataagttta tgaagaaaaa tatgtctgtc ataataggtg cgtgtgacac tttccgcgct gcagccgtgg agcaacttaa cgtttgggct gaaagaatat cttgccccat agtaacgggc actattggtg ctgatgcagc aagtgtggct tacaagacgg ttgaaagagc tgtaaaagag ccttttgatg ttgtgattat agacacggcc ggtaggctgc ataatcataa aaacttaatg gaagagttgg cgaagatatg tcgtgttatt cgccgccatg atgagaaagc cccccatgat attatactgg tattagacgc tactataggg caaaacactt tcactcnngt agaaatgttt tccagttttg tggatgtngn nggtctaatt ttgactaant tggatggcac agcta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgtcaaat gagagtttct ggcaaagcct gaagagaggg ttgtccaaaa cttctgctag gttaagtgaa ggaattagaa acatatttgg tggaaaagcc gatgttgatc aaagcacttt aaaagcctta cgtgatctac ttatctctac cgatatgggg cctgacgtag ctgatttttt tactcaaaaa atttccaccc tgaaatttac tgattcagtt gcagaagaag tacgcctaaa actcgcttct gagattgaga attttttgca accaagatct gccccgatta ccataactca tacacctcat attattcttc tatgtggagt aaatggcagc ggtaaaacaa ctactgttgc gaaattagcg cataagttta tgaagaaaaa tatgtctgtc ataataggtg cgtgtgacac tttccgcgct gcagccgtgg agcaacttaa cgtttgggct gaaagaatat cttgccccat agtaacgggc actattggtg ctgatgcagc aagtgtggct tacaagacgg ttgaaagagc tgtaaaagag ccttttgatg ttgtgattat agacacggcc ggtaggctgc ataatcataa aaacttaatg gaagagttgg cgaagatatg tcgtgttatt cgccgccatg atgagaaagc cccccatgat attatactgg tattagacgc tactataggg caaaacactt tcactcaggt agaaatgttt tccagttttg tggatgtagg cggtctaatt ttgactaagt tggatggcac agctaaaggt ggtgtagttt tgcgtgtatc acagaaatac aacttaatga tacatgctat aggaactggc gagggaatag aagatttgga agatttttct gctactaatt ttgcaaagag tttactggaa ataaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa