SSGCID: Target Page

AnphA.00520.a

phosphomethylpyrimidine kinase

CENTER ID:	AnphA.00520.a
ORGANISM:	Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE:	Human granulocytic anaplasmosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00520.a.A1.GE26674	Full length( AnphA.00520.a )		1	265

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnphA.00520.a.A1.PS00299	Full length( AnphA.00520.a )		1	265

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|212042.8.peg.1062
RefSeq:	YP_505555.1
UniProt:	Q2GJ96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYKGNVLSIA GSDSGGGAGI QADIKTITML GCYAATCITA ITAQNTQEVR GVWELAPEVV KGQLEAVLDD LKVDAIKIGM LPVGLIGVVA ESLPCEIPVI LDPVMVSTSG FELTDPMGYT ESIRPLAQNA ALVTPNIKET EALSGMKNIR TREEMVTAGN KIIQFLGIKA VLVKGCIWEE EEFVESVLIY DGKTVFFRNE RAPGELHGTG CTLTSAIACF IARGLSLEES TRLAFDYVSC TIRNAPIVGQ GLRPLFHNYF IVGDC

NT Sequence

TTGTATAAAG GTAATGTGCT TAGCATAGCT GGGTCTGATT CCGGTGGAGG AGCTGGTATT CAAGCTGACA TAAAAACCAT TACGATGTTG GGGTGCTACG CAGCTACATG CATTACCGCT ATAACGGCGC AGAATACTCA AGAAGTTAGG GGAGTGTGGG AGCTAGCTCC TGAAGTAGTA AAGGGGCAAT TGGAAGCGGT ACTGGATGAC CTCAAGGTAG ATGCTATAAA AATAGGTATG CTGCCGGTTG GTTTGATAGG GGTGGTTGCT GAAAGTCTAC CCTGCGAGAT ACCTGTGATA CTGGATCCTG TGATGGTATC GACGTCAGGT TTTGAATTAA CAGACCCTAT GGGATATACT GAAAGTATAA GACCGCTTGC ACAGAATGCT GCTTTGGTTA CACCCAATAT TAAGGAGACC GAAGCGCTCT CAGGCATGAA AAATATCCGC ACGCGGGAGG AAATGGTTAC TGCAGGTAAC AAGATAATTC AATTTCTAGG CATAAAGGCG GTTCTGGTTA AGGGTTGTAT CTGGGAAGAG GAAGAGTTTG TAGAAAGCGT ATTGATATAC GATGGCAAGA CTGTTTTCTT TAGAAATGAA AGAGCTCCTG GCGAGCTCCA TGGCACAGGA TGCACTTTAA CATCAGCCAT AGCGTGTTTC ATAGCACGAG GGCTTTCACT GGAGGAGAGT ACAAGGCTTG CATTTGACTA TGTCTCGTGT ACCATAAGAA ACGCACCCAT AGTAGGGCAG GGTTTAAGGC CTCTTTTTCA CAACTATTTT ATAGTAGGCG ATTGT

Details for AnphA.00520.a.A1.GE26674

HARVESTED ON:	5/15/2009
SEQUENCED ON:	3/21/2012
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMLYKGNV LSIAGSDSGG GAGIQADIKT ITMLGCYAAT CITAITAQNT QEVRGVWELA PEVVKGQLEA VLDDLKVDAI KIGMLPVGLI GVVAESLPCE IPVILDPVMV STSGFELTDP MGYTESIRPL AQNAALVTPN IKETEALSGM KNIRTREEMV TAGNKIIQFL GIKAVLVKGC IWEEEEFVES VLIYDGKTVF FRNERAPGEL HGTGCTLTSA IACFIARGLS LEESTRLAFD YVSCTIRNAP IVGQGLRPLF HNYFIVG

Validated NT Sequence

ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgttgtat aaaggtaatg tgcttagcat agctgggtct gattccggtg gaggagctgg tattcaagct gacataaaaa ccattacgat gttggggtgc tacgcagcta catgcattac cgctataacg gcgcagaata ctcaagaagt taggggagtg tgggagctag ctcctgaagt agtaaagggg caattggaag cggtactgga tgacctcaag gtagatgcta taaaaatagg tatgctgccg gttggtttga taggggtggt tgctgaaagt ctaccctgcg agatacctgt gatactggat cctgtgatgg tatcgacgtc aggttttgaa ttaacagacc ctatgggata tactgaaagt ataagaccgc ttgcacagaa tgctgctttg gttacaccca atattaagga gaccgaagcg ctctcaggca tgaaaaatat ccgcacgcgg gaggaaatgg ttactgcagg taacaagata attcaatttc taggcataaa ggcggttctg gttaagggtt gtatctggga agaggaagag tttgtagaaa gcgtattgat atacgatggc aagactgttt tctttagaaa tgaaagagct cctggcgagc tccatggcac aggatgcact ttaacatcag ccatagcgtg tttcatagca cgagggcttt cactggagga gagtacaagg cttgcatttg actatgtctc gtgtaccata agaaacgcac ccatagtagg gcagggttta aggcctcttt ttcacaacta ttttatagta ggcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLYKGNVLS IAGSDSGGGA GIQADIKTIT MLGCYAATCI TAITAQNTQE VRGVWELAPE VVKGQLEAVL DDLKVDAIKI GMLPVGLIGV VAESLPCEIP VILDPVMVST SGFELTDPMG YTESIRPLAQ NAALVTPNIK ETEALSGMKN IRTREEMVTA GNKIIQFLGI KAVLVKGCIW EEEEFVESVL IYDGKTVFFR NERAPGELHG TGCTLTSAIA CFIARGLSLE ESTRLAFDYV SCTIRNAPIV GQGLRPLFHN YFIVGDC

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgtataaa ggtaatgtgc ttagcatagc tgggtctgat tccggtggag gagctggtat tcaagctgac ataaaaacca ttacgatgtt ggggtgctac gcagctacat gcattaccgc tataacggcg cagaatactc aagaagttag gggagtgtgg gagctagctc ctgaagtagt aaaggggcaa ttggaagcgg tactggatga cctcaaggta gatgctataa aaataggtat gctgccggtt ggtttgatag gggtggttgc tgaaagtcta ccctgcgaga tacctgtgat actggatcct gtgatggtat cgacgtcagg ttttgaatta acagacccta tgggatatac tgaaagtata agaccgcttg cacagaatgc tgctttggtt acacccaata ttaaggagac cgaagcgctc tcaggcatga aaaatatccg cacgcgggag gaaatggtta ctgcaggtaa caagataatt caatttctag gcataaaggc ggttctggtt aagggttgta tctgggaaga ggaagagttt gtagaaagcg tattgatata cgatggcaag actgttttct ttagaaatga aagagctcct ggcgagctcc atggcacagg atgcacttta acatcagcca tagcgtgttt catagcacga gggctttcac tggaggagag tacaaggctt gcatttgact atgtctcgtg taccataaga aacgcaccca tagtagggca gggtttaagg cctctttttc acaactattt tatagtaggc gattgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for AnphA.00520.a.A1.PS00299

PURIFICATION DATe:	7/21/2009
CONCENTRATION:	34.01mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	14
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGTATAAA GGTAATGTGC TTAGCATAGC TGGGTCTGAT TCCGGTGGAG GAGCTGGTAT TCAAGCTGAC ATAAAAACCA TTACGATGTT GGGGTGCTAC GCAGCTACAT GCATTACCGC TATAACGGCG CAGAATACTC AAGAAGTTAG GGGAGTGTGG GAGCTAGCTC CTGAAGTAGT AAAGGGGCAA TTGGAAGCGG TACTGGATGA CCTCAAGGTA GATGCTATAA AAATAGGTAT GCTGCCGGTT GGTTTGATAG GGGTGGTTGC TGAAAGTCTA CCCTGCGAGA TACCTGTGAT ACTGGATCCT GTGATGGTAT CGACGTCAGG TTTTGAATTA ACAGACCCTA TGGGATATAC TGAAAGTATA AGACCGCTTG CACAGAATGC TGCTTTGGTT ACACCCAATA TTAAGGAGAC CGAAGCGCTC TCAGGCATGA AAAATATCCG CACGCGGGAG GAAATGGTTA CTGCAGGTAA CAAGATAATT CAATTTCTAG GCATAAAGGC GGTTCTGGTT AAGGGTTGTA TCTGGGAAGA GGAAGAGTTT GTAGAAAGCG TATTGATATA CGATGGCAAG ACTGTTTTCT TTAGAAATGA AAGAGCTCCT GGCGAGCTCC ATGGCACAGG ATGCACTTTA ACATCAGCCA TAGCGTGTTT CATAGCACGA GGGCTTTCAC TGGAGGAGAG TACAAGGCTT GCATTTGACT ATGTCTCGTG TACCATAAGA AACGCACCCA TAGTAGGGCA GGGTTTAAGG CCTCTTTTTC ACAACTATTT TATAGTAGGC GATTGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA