AnphA.00481.a

methionine aminopeptidase

CENTER ID: AnphA.00481.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00481.a.A1.GE26632 Full length( AnphA.00481.a ) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00481.a.A1.PS00238 Full length( AnphA.00481.a ) 1 264

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_126721
PATRIC ID: fig|212042.8.peg.1288
RefSeq: YP_505732.1
UniProt: Q2GIR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRGTITIHS KEDFACMRRA GMLAAKVLDF ITPHVVPGVT TNALNDLCHD FIISAGAIPA PLGYRGYPKS ICTSKNFVVC HGIPDDIALK NGDIVNIDVT VILDGWHGDT SRMYWVGDNV SIKAKRICEA SYKALMAAIG VIQPGKKLNS IGLAIEEEIR GYGYSIVRDY CGHGIGREFH AAPNIVHYYD EEDDVTIQEG MFFTVEPMIN AGKYHTVLDK KDGWTVTTRD FSLSAQFEHT LGVTETGVEI FTMSPKNWHC PPYL
NT Sequence
ATGGGGCGGG GTACAATAAC CATCCACTCC AAAGAGGATT TTGCCTGTAT GAGAAGGGCT GGGATGCTTG CAGCTAAGGT GCTTGATTTT ATAACGCCGC ATGTTGTTCC TGGTGTGACT ACTAATGCTC TGAATGATCT ATGTCACGAT TTCATCATTT CTGCCGGGGC TATTCCAGCG CCTTTGGGCT ATAGAGGGTA TCCTAAGTCT ATTTGTACTT CGAAGAATTT TGTGGTTTGC CATGGCATTC CAGATGATAT TGCATTAAAA AACGGCGATA TAGTTAACAT AGACGTTACT GTGATCCTCG ATGGTTGGCA CGGGGATACT AGTAGGATGT ATTGGGTTGG TGATAACGTC TCTATTAAGG CTAAGCGCAT TTGTGAGGCA AGTTATAAGG CATTGATGGC GGCGATTGGT GTAATACAGC CAGGTAAGAA GCTCAATAGC ATAGGGTTAG CTATAGAGGA AGAAATCAGA GGTTATGGAT ACTCCATTGT TAGAGATTAC TGCGGACATG GGATAGGTCG CGAATTTCAT GCTGCTCCTA ACATAGTTCA CTACTATGAC GAAGAGGATG ATGTTACGAT TCAGGAGGGA ATGTTTTTCA CTGTTGAGCC AATGATCAAT GCTGGAAAGT ATCATACTGT GCTAGATAAG AAAGACGGAT GGACAGTTAC AACGAGAGAC TTTTCCCTTT CAGCGCAGTT TGAACATACC TTGGGTGTAA CTGAAACTGG CGTTGAGATT TTTACTATGT CGCCAAAAAA TTGGCATTGT CCGCCATACC TT
Details for AnphA.00481.a.A1.GE26632
HARVESTED ON: 5/15/2009
SEQUENCED ON: 6/18/2009
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGRGTITIH SKEDFACMRR AGMLAAKVLD FITPHVVPGV TTNALNDLCH DFIISAGAIP APLGYRGYPK SICTSKNFVV CHGIPDDIAL KNGDIVNIDV TVILDGWHGD TSRMYWVGDN VSIKAKRICE ASYKALMAAI GVIQPGKKLN SIGLAIEEEI RGYGYSIVRD YCGHGIGREF HAAPNIVHYY DEEDDVTIQE GMFFTVEPMI NAGKYHTVLD KKDGWTVTTR DFSLSAQFEH TLGVTETGVE IFTMSPKNWH CPP
Validated NT Sequence
ttaagaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggggcgg ggtacaataa ccatccactc caaagaggat tttgcctgta tgagaagggc tgggatgctt gcagctaagg tgcttgattt tataacgccg catgttgttc ctggtgtgac tactaatgct ctgaatgatc tatgtcacga tttcatcatt tctgccgggg ctattccagc gcctttgggc tatagagggt atcctaagtc tatttgtact tcgaagaatt ttgtggtttg ccatggcatt ccagatgata ttgcattaaa aaacggcgat atagttaaca tagacgttac tgtgatcctc gatggttggc acggggatac tagtaggatg tattgggttg gtgataacgt ctctattaag gctaagcgca tttgtgaggc aagttataag gcattgatgg cggcgattgg tgtaatacag ccaggtaaga agctcaatag catagggtta gctatagagg aagaaatcag aggttatgga tactccattg ttagagatta ctgcggacat gggataggtc gcgaatttca tgctgctcct aacatagttc actactatga cgaagaggat gatgttacga ttcaggaggg aatgtttttc actgttgagc caatgatcaa tgctggaaag tatcatactg tgctagataa gaaagacgga tggacagtta caacgagaga cttttccctt tcagcgcagt ttgaacatac cttgggtgta actgaaactg gcgttgagat ttttactatg tcgccaaaaa attggcattg tccgccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRGTITIH SKEDFACMRR AGMLAAKVLD FITPHVVPGV TTNALNDLCH DFIISAGAIP APLGYRGYPK SICTSKNFVV CHGIPDDIAL KNGDIVNIDV TVILDGWHGD TSRMYWVGDN VSIKAKRICE ASYKALMAAI GVIQPGKKLN SIGLAIEEEI RGYGYSIVRD YCGHGIGREF HAAPNIVHYY DEEDDVTIQE GMFFTVEPMI NAGKYHTVLD KKDGWTVTTR DFSLSAQFEH TLGVTETGVE IFTMSPKNWH CPPYL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggggcggggt acaataacca tccactccaa agaggatttt gcctgtatga gaagggctgg gatgcttgca gctaaggtgc ttgattttat aacgccgcat gttgttcctg gtgtgactac taatgctctg aatgatctat gtcacgattt catcatttct gccggggcta ttccagcgcc tttgggctat agagggtatc ctaagtctat ttgtacttcg aagaattttg tggtttgcca tggcattcca gatgatattg cattaaaaaa cggcgatata gttaacatag acgttactgt gatcctcgat ggttggcacg gggatactag taggatgtat tgggttggtg ataacgtctc tattaaggct aagcgcattt gtgaggcaag ttataaggca ttgatggcgg cgattggtgt aatacagcca ggtaagaagc tcaatagcat agggttagct atagaggaag aaatcagagg ttatggatac tccattgtta gagattactg cggacatggg ataggtcgcg aatttcatgc tgctcctaac atagttcact actatgacga agaggatgat gttacgattc aggagggaat gtttttcact gttgagccaa tgatcaatgc tggaaagtat catactgtgc tagataagaa agacggatgg acagttacaa cgagagactt ttccctttca gcgcagtttg aacatacctt gggtgtaact gaaactggcg ttgagatttt tactatgtcg ccaaaaaatt ggcattgtcc gccatacctt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for AnphA.00481.a.A1.PS00238
PURIFICATION DATe: 7/14/2009
CONCENTRATION: 49.53mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGRGTITIH SKEDFACMRR AGMLAAKVLD FITPHVVPGV TTNALNDLCH DFIISAGAIP APLGYRGYPK SICTSKNFVV CHGIPDDIAL KNGDIVNIDV TVILDGWHGD TSRMYWVGDN VSIKAKRICE ASYKALMAAI GVIQPGKKLN SIGLAIEEEI RGYGYSIVRD YCGHGIGREF HAAPNIVHYY DEEDDVTIQE GMFFTVEPMI NAGKYHTVLD KKDGWTVTTR DFSLSAQFEH TLGVTETGVE IFTMSPKNWH CPP
Validated NT Sequence
ttaagaagga gnnataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggggcgg ggtacaataa ccatccactc caaagaggat tttgcctgta tgagaagggc tgggatgctt gcagctaagg tgcttgattt tataacgccg catgttgttc ctggtgtgac tactaatgct ctgaatgatc tatgtcacga tttcatcatt tctgccgggg ctattccagc gcctttgggc tatagagggt atcctaagtc tatttgtact tcgaagaatt ttgtggtttg ccatggcatt ccagatgata ttgcattaaa aaacggcgat atagttaaca tagacgttac tgtgatcctc gatggttggc acggggatac tagtaggatg tattgggttg gtgataacgt ctctattaag gctaagcgca tttgtgaggc aagttataag gcattgatgg cggcgattgg tgtaatacag ccaggtaaga agctcaatag catagggtta gctatagagg aagaaatcag aggttatgga tactccattg ttagagatta ctgcggacat gggataggtc gcgaatttca tgctgctcct aacatagttc actactatga cgaagaggat gatgttacga ttcaggaggg aatgtttttc actgttgagc caatgatcaa tgctggaaag tatcatactg tgctagataa gaaagacgga tggacagtta caacgagaga cttttccctt tcagcgcagt ttgaacatac cttgggtgta actgaaactg gcgttgagat ttttactatg tcgccaaaaa attggcattg tccgccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGRGTITIH SKEDFACMRR AGMLAAKVLD FITPHVVPGV TTNALNDLCH DFIISAGAIP APLGYRGYPK SICTSKNFVV CHGIPDDIAL KNGDIVNIDV TVILDGWHGD TSRMYWVGDN VSIKAKRICE ASYKALMAAI GVIQPGKKLN SIGLAIEEEI RGYGYSIVRD YCGHGIGREF HAAPNIVHYY DEEDDVTIQE GMFFTVEPMI NAGKYHTVLD KKDGWTVTTR DFSLSAQFEH TLGVTETGVE IFTMSPKNWH CPPYL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGGCGGGGT ACAATAACCA TCCACTCCAA AGAGGATTTT GCCTGTATGA GAAGGGCTGG GATGCTTGCA GCTAAGGTGC TTGATTTTAT AACGCCGCAT GTTGTTCCTG GTGTGACTAC TAATGCTCTG AATGATCTAT GTCACGATTT CATCATTTCT GCCGGGGCTA TTCCAGCGCC TTTGGGCTAT AGAGGGTATC CTAAGTCTAT TTGTACTTCG AAGAATTTTG TGGTTTGCCA TGGCATTCCA GATGATATTG CATTAAAAAA CGGCGATATA GTTAACATAG ACGTTACTGT GATCCTCGAT GGTTGGCACG GGGATACTAG TAGGATGTAT TGGGTTGGTG ATAACGTCTC TATTAAGGCT AAGCGCATTT GTGAGGCAAG TTATAAGGCA TTGATGGCGG CGATTGGTGT AATACAGCCA GGTAAGAAGC TCAATAGCAT AGGGTTAGCT ATAGAGGAAG AAATCAGAGG TTATGGATAC TCCATTGTTA GAGATTACTG CGGACATGGG ATAGGTCGCG AATTTCATGC TGCTCCTAAC ATAGTTCACT ACTATGACGA AGAGGATGAT GTTACGATTC AGGAGGGAAT GTTTTTCACT GTTGAGCCAA TGATCAATGC TGGAAAGTAT CATACTGTGC TAGATAAGAA AGACGGATGG ACAGTTACAA CGAGAGACTT TTCCCTTTCA GCGCAGTTTG AACATACCTT GGGTGTAACT GAAACTGGCG TTGAGATTTT TACTATGTCG CCAAAAAATT GGCATTGTCC GCCATACCTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA