AnphA.00430.a

tryptophanyl-tRNA synthetase

CENTER ID: AnphA.00430.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00430.a.A1.GE26628 Full length( AnphA.00430.a ) 1 331
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00430.a.A1.PW26702 Full length( AnphA.00430.a ) 1 331
AnphA.00430.a.A1.PW27577 Full length( AnphA.00430.a ) 1 331

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.30
RefSeq: YP_504669.1
UniProt: Q2GLT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFSAVQPSG GSLHLGNYLG AVKKWVALQD AGKCVFCIVD MHALTSGSAE TLSIRANTLS LLASYIACGI DPAKAVVFLQ SSVPEHAELC WILGCLTPVG WLNRMTQFKD KSRSDSYRAN LGLYSYPVLM AADILLYKAD LVPVGNDQKQ HLELAQSIAR TFNTIYGVDF FCIPEAMPFD SAARIMSLKT GTKKMSKSDP SDFSRINLSD DNDVIALKIK KATTDSATGF CYGGLNQRPE VNNLVNIFAA LSDSEPREVC VRFTNSSNKD FKDALTELLI ERISPIRTKT RELLDDPGYL NSILSSGNEQ ARSIAGKNLR EVKEIIGLYS V
NT Sequence
ATGATTTTTT CTGCCGTACA GCCCAGTGGT GGCTCGTTAC ATCTGGGCAA TTATCTTGGA GCAGTGAAGA AGTGGGTTGC GTTGCAGGAC GCCGGCAAGT GCGTGTTTTG CATCGTGGAC ATGCATGCTT TAACCTCGGG AAGTGCAGAA ACACTGAGTA TACGGGCAAA TACGCTCAGT TTATTAGCTT CTTATATCGC TTGTGGCATA GATCCTGCAA AAGCGGTAGT TTTTTTACAG TCGTCTGTTC CTGAACATGC AGAGCTCTGC TGGATACTAG GATGTTTGAC GCCCGTGGGG TGGTTGAATA GGATGACACA GTTCAAGGAC AAGAGCCGTT CAGATTCCTA CCGGGCTAAT TTGGGTCTAT ACAGTTACCC TGTGCTTATG GCTGCAGACA TTCTTTTGTA TAAAGCGGAT TTGGTTCCTG TAGGAAATGA TCAAAAACAA CACTTGGAGC TTGCACAGAG TATTGCTAGG ACGTTCAATA CTATTTATGG AGTTGACTTT TTTTGCATTC CGGAAGCAAT GCCTTTTGAT TCCGCCGCTA GGATCATGAG CTTGAAAACG GGTACAAAGA AGATGAGCAA GTCCGACCCT TCTGACTTCT CTCGTATAAA TTTGAGTGAT GATAACGATG TTATAGCCTT GAAGATAAAA AAGGCTACAA CCGATTCTGC AACTGGCTTT TGCTATGGAG GACTCAACCA GCGGCCAGAG GTGAACAATC TCGTAAACAT ATTTGCAGCA CTATCTGATT CAGAACCCAG GGAGGTGTGT GTAAGGTTTA CAAACAGTAG CAATAAAGAT TTTAAAGATG CGTTAACGGA GTTACTGATC GAAAGAATAT CGCCCATTAG AACCAAGACC AGGGAATTAC TGGACGATCC TGGTTATCTC AACTCCATAC TTTCCTCTGG AAATGAACAA GCTCGGTCAA TCGCGGGTAA AAATCTGCGG GAAGTGAAGG AGATCATAGG TTTGTACAGT GTG
Details for AnphA.00430.a.A1.GE26628
HARVESTED ON: 5/15/2009
SEQUENCED ON: 6/18/2009
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIFSAVQPS GGSLHLGNYL GAVKKWVALQ DAGKCVFCIV DMHALTSGSA ETLSIRANTL SLLASYIACG IDPAKAVVFL QSSVPEHAEL CWILGCLTPV GWLNRMTQFK DKSRSDSYRA NLGLYSYPVL MAADILLYKA DLVPVGNDQK QHLELAQSIA RTFNTIYGVD FFCIPEAMPF DSAARIMSLK TGTKKMSKSD PSDFSRINLS DDNDVIALKI KKATTDSATG FCYGGLNQRP EVNNLVNIFA ALSDSEPREV CVRFTNSSNK DFKDALTELL IERISPIRTK TRELLDDPGY LNSILSSGNE QARSIAGKNL REVKEIIGLY SV
Validated NT Sequence
ttattacaca ctgtacaaac ctatgatctc cttcacttcc cgcagatttt tacccgcgat tgaccgagct tgttcatttc cagaggaaag tatggagttg agataaccag gatcgtccag taattccctg gtcttggttc taatgggcga tattctttcg atcagtaact ccgttaacgc atctttaaaa tctttattgc tactgtttgt aaaccttaca cacacctccc tgggttctga atcagatagt gctgcaaata tgtttacgag attgttcacc tctggccgct ggttgagtcc tccatagcaa aagccagttg cagaatcggt tgtagccttt tttatcttca aggctataac atcgttatca tcactcaaat ttatacgaga gaagtcagaa gggtcggact tgctcatctt ctttgtaccc gttttcaagc tcatgatcct agcggcggaa tcaaaaggca ttgcttccgg aatgcaaaaa aagtcaactc cataaatagt attgaacgtc ctagcaatac tctgtgcaag ctccaagtgt tgtttttgat catttcctac aggaaccaaa tccgctttat acaaaagaat gtctgcagcc ataagcacag ggtaactgta tagacccaaa ttagcccggt aggaatctga acggctcttg tccttgaact gtgtcatcct attcaaccac cccacgggcg tcaaacatcc tagtatccag cagagctctg catgttcagg aacagacgac tgtaaaaaaa ctaccgcttt tgcaggatct atgccacaag cgatataaga agctaataaa ctgagcgtat ttgcccgtat actcagtgtt tctgcacttc ccgaggttaa agcatgcatg tccacgatgc aaaacacgca cttgccggcg tcctgcaacg caacccactt cttcactgct ccaagataat tgcccagatg taacgagcca ccactgggct gtacggcaga aaaaatcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIFSAVQPS GGSLHLGNYL GAVKKWVALQ DAGKCVFCIV DMHALTSGSA ETLSIRANTL SLLASYIACG IDPAKAVVFL QSSVPEHAEL CWILGCLTPV GWLNRMTQFK DKSRSDSYRA NLGLYSYPVL MAADILLYKA DLVPVGNDQK QHLELAQSIA RTFNTIYGVD FFCIPEAMPF DSAARIMSLK TGTKKMSKSD PSDFSRINLS DDNDVIALKI KKATTDSATG FCYGGLNQRP EVNNLVNIFA ALSDSEPREV CVRFTNSSNK DFKDALTELL IERISPIRTK TRELLDDPGY LNSILSSGNE QARSIAGKNL REVKEIIGLY SV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattttttct gccgtacagc ccagtggtgg ctcgttacat ctgggcaatt atcttggagc agtgaagaag tgggttgcgt tgcaggacgc cggcaagtgc gtgttttgca tcgtggacat gcatgcttta acctcgggaa gtgcagaaac actgagtata cgggcaaata cgctcagttt attagcttct tatatcgctt gtggcataga tcctgcaaaa gcggtagttt ttttacagtc gtctgttcct gaacatgcag agctctgctg gatactagga tgtttgacgc ccgtggggtg gttgaatagg atgacacagt tcaaggacaa gagccgttca gattcctacc gggctaattt gggtctatac agttaccctg tgcttatggc tgcagacatt cttttgtata aagcggattt ggttcctgta ggaaatgatc aaaaacaaca cttggagctt gcacagagta ttgctaggac gttcaatact atttatggag ttgacttttt ttgcattccg gaagcaatgc cttttgattc cgccgctagg atcatgagct tgaaaacggg tacaaagaag atgagcaagt ccgacccttc tgacttctct cgtataaatt tgagtgatga taacgatgtt atagccttga agataaaaaa ggctacaacc gattctgcaa ctggcttttg ctatggagga ctcaaccagc ggccagaggt gaacaatctc gtaaacatat ttgcagcact atctgattca gaacccaggg aggtgtgtgt aaggtttaca aacagtagca ataaagattt taaagatgcg ttaacggagt tactgatcga aagaatatcg cccattagaa ccaagaccag ggaattactg gacgatcctg gttatctcaa ctccatactt tcctctggaa atgaacaagc tcggtcaatc gcgggtaaaa atctgcggga agtgaaggag atcataggtt tgtacagtgt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for AnphA.00430.a.A1.PW26702
PURIFICATION DATe: 6/29/2009
CONCENTRATION: 29.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIFSAVQPS GGSLHLGNYL GAVKKWVALQ DAGKCVFCIV DMHALTSGSA ETLSIRANTL SLLASYIACG IDPAKAVVFL QSSVPEHAEL CWILGCLTPV GWLNRMTQFK DKSRSDSYRA NLGLYSYPVL MAADILLYKA DLVPVGNDQK QHLELAQSIA RTFNTIYGVD FFCIPEAMPF DSAARIMSLK TGTKKMSKSD PSDFSRINLS DDNDVIALKI KKATTDSATG FCYGGLNQRP EVNNLVNIFA ALSDSEPREV CVRFTNSSNK DFKDALTELL IERISPIRTK TRELLDDPGY LNSILSSGNE QARSIAGKNL REVKEIIGLY SV
Validated NT Sequence
ttattacaca ctgtacaaac ctatgatctc cttcacttcc cgcagatttt tacccgcgat tgaccgagct tgttcatttc cagaggaaag tatggagttg agataaccag gatcgtccag taattccctg gtcttggttc taatgggcga tattctttcg atcagtaact ccgttaacgc atctttaaaa tctttattgc tactgtttgt aaaccttaca cacacctccc tgggttctga atcagatagt gctgcaaata tgtttacgag attgttcacc tctggccgct ggttgagtcc tccatagcaa aagccagttg cagaatcggt tgtagccttt tttatcttca aggctataac atcgttatca tcactcaaat ttatacgaga gaagtcagaa gggtcggact tgctcatctt ctttgtaccc gttttcaagc tcatgatcct agcggcggaa tcaaaaggca ttgcttccgg aatgcaaaaa aagtcaactc cataaatagt attgaacgtc ctagcaatac tctgtgcaag ctccaagtgt tgtttttgat catttcctac aggaaccaaa tccgctttat acaaaagaat gtctgcagcc ataagcacag ggtaactgta tagacccaaa ttagcccggt aggaatctga acggctcttg tccttgaact gtgtcatcct attcaaccac cccacgggcg tcaaacatcc tagtatccag cagagctctg catgttcagg aacagacgac tgtaaaaaaa ctaccgcttt tgcaggatct atgccacaag cgatataaga agctaataaa ctgagcgtat ttgcccgtat actcagtgtt tctgcacttc ccgaggttaa agcatgcatg tccacgatgc aaaacacgca cttgccggcg tcctgcaacg caacccactt cttcactgct ccaagataat tgcccagatg taacgagcca ccactgggct gtacggcaga aaaaatcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIFSAVQPS GGSLHLGNYL GAVKKWVALQ DAGKCVFCIV DMHALTSGSA ETLSIRANTL SLLASYIACG IDPAKAVVFL QSSVPEHAEL CWILGCLTPV GWLNRMTQFK DKSRSDSYRA NLGLYSYPVL MAADILLYKA DLVPVGNDQK QHLELAQSIA RTFNTIYGVD FFCIPEAMPF DSAARIMSLK TGTKKMSKSD PSDFSRINLS DDNDVIALKI KKATTDSATG FCYGGLNQRP EVNNLVNIFA ALSDSEPREV CVRFTNSSNK DFKDALTELL IERISPIRTK TRELLDDPGY LNSILSSGNE QARSIAGKNL REVKEIIGLY SV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTTTTTCT GCCGTACAGC CCAGTGGTGG CTCGTTACAT CTGGGCAATT ATCTTGGAGC AGTGAAGAAG TGGGTTGCGT TGCAGGACGC CGGCAAGTGC GTGTTTTGCA TCGTGGACAT GCATGCTTTA ACCTCGGGAA GTGCAGAAAC ACTGAGTATA CGGGCAAATA CGCTCAGTTT ATTAGCTTCT TATATCGCTT GTGGCATAGA TCCTGCAAAA GCGGTAGTTT TTTTACAGTC GTCTGTTCCT GAACATGCAG AGCTCTGCTG GATACTAGGA TGTTTGACGC CCGTGGGGTG GTTGAATAGG ATGACACAGT TCAAGGACAA GAGCCGTTCA GATTCCTACC GGGCTAATTT GGGTCTATAC AGTTACCCTG TGCTTATGGC TGCAGACATT CTTTTGTATA AAGCGGATTT GGTTCCTGTA GGAAATGATC AAAAACAACA CTTGGAGCTT GCACAGAGTA TTGCTAGGAC GTTCAATACT ATTTATGGAG TTGACTTTTT TTGCATTCCG GAAGCAATGC CTTTTGATTC CGCCGCTAGG ATCATGAGCT TGAAAACGGG TACAAAGAAG ATGAGCAAGT CCGACCCTTC TGACTTCTCT CGTATAAATT TGAGTGATGA TAACGATGTT ATAGCCTTGA AGATAAAAAA GGCTACAACC GATTCTGCAA CTGGCTTTTG CTATGGAGGA CTCAACCAGC GGCCAGAGGT GAACAATCTC GTAAACATAT TTGCAGCACT ATCTGATTCA GAACCCAGGG AGGTGTGTGT AAGGTTTACA AACAGTAGCA ATAAAGATTT TAAAGATGCG TTAACGGAGT TACTGATCGA AAGAATATCG CCCATTAGAA CCAAGACCAG GGAATTACTG GACGATCCTG GTTATCTCAA CTCCATACTT TCCTCTGGAA ATGAACAAGC TCGGTCAATC GCGGGTAAAA ATCTGCGGGA AGTGAAGGAG ATCATAGGTT TGTACAGTGT GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for AnphA.00430.a.A1.PW27577
PURIFICATION DATe: 9/8/2009
CONCENTRATION: 1.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIFSAVQPS GGSLHLGNYL GAVKKWVALQ DAGKCVFCIV DMHALTSGSA ETLSIRANTL SLLASYIACG IDPAKAVVFL QSSVPEHAEL CWILGCLTPV GWLNRMTQFK DKSRSDSYRA NLGLYSYPVL MAADILLYKA DLVPVGNDQK QHLELAQSIA RTFNTIYGVD FFCIPEAMPF DSAARIMSLK TGTKKMSKSD PSDFSRINLS DDNDVIALKI KKATTDSATG FCYGGLNQRP EVNNLVNIFA ALSDSEPREV CVRFTNSSNK DFKDALTELL IERISPIRTK TRELLDDPGY LNSILSSGNE QARSIAGKNL REVKEIIGLY SV
Validated NT Sequence
ttattacaca ctgtacaaac ctatgatctc cttcacttcc cgcagatttt tacccgcgat tgaccgagct tgttcatttc cagaggaaag tatggagttg agataaccag gatcgtccag taattccctg gtcttggttc taatgggcga tattctttcg atcagtaact ccgttaacgc atctttaaaa tctttattgc tactgtttgt aaaccttaca cacacctccc tgggttctga atcagatagt gctgcaaata tgtttacgag attgttcacc tctggccgct ggttgagtcc tccatagcaa aagccagttg cagaatcggt tgtagccttt tttatcttca aggctataac atcgttatca tcactcaaat ttatacgaga gaagtcagaa gggtcggact tgctcatctt ctttgtaccc gttttcaagc tcatgatcct agcggcggaa tcaaaaggca ttgcttccgg aatgcaaaaa aagtcaactc cataaatagt attgaacgtc ctagcaatac tctgtgcaag ctccaagtgt tgtttttgat catttcctac aggaaccaaa tccgctttat acaaaagaat gtctgcagcc ataagcacag ggtaactgta tagacccaaa ttagcccggt aggaatctga acggctcttg tccttgaact gtgtcatcct attcaaccac cccacgggcg tcaaacatcc tagtatccag cagagctctg catgttcagg aacagacgac tgtaaaaaaa ctaccgcttt tgcaggatct atgccacaag cgatataaga agctaataaa ctgagcgtat ttgcccgtat actcagtgtt tctgcacttc ccgaggttaa agcatgcatg tccacgatgc aaaacacgca cttgccggcg tcctgcaacg caacccactt cttcactgct ccaagataat tgcccagatg taacgagcca ccactgggct gtacggcaga aaaaatcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIFSAVQPS GGSLHLGNYL GAVKKWVALQ DAGKCVFCIV DMHALTSGSA ETLSIRANTL SLLASYIACG IDPAKAVVFL QSSVPEHAEL CWILGCLTPV GWLNRMTQFK DKSRSDSYRA NLGLYSYPVL MAADILLYKA DLVPVGNDQK QHLELAQSIA RTFNTIYGVD FFCIPEAMPF DSAARIMSLK TGTKKMSKSD PSDFSRINLS DDNDVIALKI KKATTDSATG FCYGGLNQRP EVNNLVNIFA ALSDSEPREV CVRFTNSSNK DFKDALTELL IERISPIRTK TRELLDDPGY LNSILSSGNE QARSIAGKNL REVKEIIGLY SV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATTTTTTCT GCCGTACAGC CCAGTGGTGG CTCGTTACAT CTGGGCAATT ATCTTGGAGC AGTGAAGAAG TGGGTTGCGT TGCAGGACGC CGGCAAGTGC GTGTTTTGCA TCGTGGACAT GCATGCTTTA ACCTCGGGAA GTGCAGAAAC ACTGAGTATA CGGGCAAATA CGCTCAGTTT ATTAGCTTCT TATATCGCTT GTGGCATAGA TCCTGCAAAA GCGGTAGTTT TTTTACAGTC GTCTGTTCCT GAACATGCAG AGCTCTGCTG GATACTAGGA TGTTTGACGC CCGTGGGGTG GTTGAATAGG ATGACACAGT TCAAGGACAA GAGCCGTTCA GATTCCTACC GGGCTAATTT GGGTCTATAC AGTTACCCTG TGCTTATGGC TGCAGACATT CTTTTGTATA AAGCGGATTT GGTTCCTGTA GGAAATGATC AAAAACAACA CTTGGAGCTT GCACAGAGTA TTGCTAGGAC GTTCAATACT ATTTATGGAG TTGACTTTTT TTGCATTCCG GAAGCAATGC CTTTTGATTC CGCCGCTAGG ATCATGAGCT TGAAAACGGG TACAAAGAAG ATGAGCAAGT CCGACCCTTC TGACTTCTCT CGTATAAATT TGAGTGATGA TAACGATGTT ATAGCCTTGA AGATAAAAAA GGCTACAACC GATTCTGCAA CTGGCTTTTG CTATGGAGGA CTCAACCAGC GGCCAGAGGT GAACAATCTC GTAAACATAT TTGCAGCACT ATCTGATTCA GAACCCAGGG AGGTGTGTGT AAGGTTTACA AACAGTAGCA ATAAAGATTT TAAAGATGCG TTAACGGAGT TACTGATCGA AAGAATATCG CCCATTAGAA CCAAGACCAG GGAATTACTG GACGATCCTG GTTATCTCAA CTCCATACTT TCCTCTGGAA ATGAACAAGC TCGGTCAATC GCGGGTAAAA ATCTGCGGGA AGTGAAGGAG ATCATAGGTT TGTACAGTGT GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA