AnphA.00316.a
tRNA (guanine-N1)-methyltransferase
| CENTER ID: | AnphA.00316.a |
| ORGANISM: | Anaplasma phagocytophilum HZ |
| ASSOCIATED DISEASE: | Human granulocytic anaplasmosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 1 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| AnphA.00316.a.A1.GE26664 | Full length( AnphA.00316.a ) | 1 | 232 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3KNU
DEPOSITED: 11/12/2009
DETERMINATION: XRay
CLONE: AnphA.00316.a.A1.GE26664
PROTEIN: AnphA.00316.a.A1.PS00323
4IG6
DEPOSITED: 12/16/2012
DETERMINATION: XRay
CLONE: AnphA.00316.a.A1.GE26664
PROTEIN: AnphA.00316.a.A1.PS00323
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132581 |
| PATRIC ID: | fig|212042.8.peg.1358 |
| RefSeq: | YP_505786.1 |
| UniProt: | Q2GIL5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIFNVLTIFP QMFPGPLGVS NLGSALKKGL WTLNVFDIRA FANNKHNTVD DTPYGGGPGM LLRADVLGRC IDEVLSLHPN TKLMFTSPRG VSFTQDIARQ TMNFDNITLL CGRFEGIDER VVDFYKLQEV SIGDYVLSGG ELAAMVIIDT CVRMVPGVIG NAESLKQESM EGSLEYPQYT RPASWKGMEV PEVLLTGNHG EIEKWRRNAS LSITAARRPD LLKDRYGEND VE
NT Sequence
ATGATCTTCA ACGTTCTAAC AATCTTTCCG CAAATGTTTC CCGGCCCTCT TGGCGTATCT AATCTGGGTT CTGCTCTGAA AAAAGGGCTG TGGACGTTGA ACGTTTTTGA TATTAGAGCC TTTGCGAATA ATAAACATAA TACTGTTGAT GATACCCCTT ATGGAGGGGG GCCAGGCATG CTTCTCAGGG CCGATGTGTT GGGAAGATGC ATAGATGAAG TTTTGTCTCT GCATCCGAAT ACTAAACTTA TGTTTACTTC TCCTCGAGGT GTAAGTTTTA CTCAAGATAT CGCTAGACAA ACTATGAATT TTGACAATAT AACCTTACTC TGCGGGCGCT TTGAAGGTAT TGATGAGAGA GTGGTAGATT TTTACAAATT GCAGGAAGTT AGCATAGGAG ATTATGTTCT CTCTGGTGGT GAGTTAGCTG CAATGGTAAT TATCGACACG TGTGTTAGAA TGGTGCCAGG AGTTATAGGT AATGCAGAGA GCTTAAAGCA GGAAAGTATG GAGGGTAGCC TTGAATACCC CCAGTATACT AGGCCAGCTT CCTGGAAGGG GATGGAGGTT CCAGAGGTCC TTCTTACTGG GAATCATGGA GAAATAGAAA AGTGGAGACG AAATGCTTCT CTTTCGATTA CTGCTGCAAG ACGACCGGAC TTGCTAAAAG ATAGATATGG TGAAAATGAC GTGGAA
Details for AnphA.00316.a.A1.GE26664
| HARVESTED ON: | 5/15/2009 |
| SEQUENCED ON: | 6/18/2009 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIFNVLTIF PQMFPGPLGV SNLGSALKKG LWTLNVFDIR AFANNKHNTV DDTPYGGGPG MLLRADVLGR CIDEVLSLHP NTKLMFTSPR GVSFTQDIAR QTMNFDNITL LCGRFEGIDE RVVDFYKLQE VSIGDYVLSG GELAAMVIID TCVRMVPGVI GNAESLKQES MEGSLEYPQY TRPASWKGME VPEVLLTGNH GEIEKWRRNA SLSITAARRP DLLKDRYGEN DVE
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgatct tcaacgttct aacaatcttt ccgcaaatgt ttcccggccc tcttggcgta tctaatctgg gttctgctct gaaaaaaggg ctgtggacgt tgaacgtttt tgatattaga gcctttgcga ataataaaca taatactgtt gatgataccc cttatggagg ggggccaggc atgcttctca gggccgatgt gttgggaaga tgcatagatg aagttttgtc tctgcatccg aatactaaac ttatgtttac ttctcctcga ggtgtaagtt ttactcaaga tatcgctaga caaactatga attttgacaa tataacctta ctctgcgggc gctttgaagg tattgatgag agagtggtag atttttacaa attgcaggaa gttagcatag gagattatgt tctctctggt ggtgagttag ctgcaatggt aattatcgac acgtgtgtta gaatggtgcc aggagttata ggtaatgcag agagcttaaa gcaggaaagt atggagggta gccttgaata cccccagtat actaggccag cttcctggaa ggggatggag gttccagagg tccttcttac tgggaatcat ggagaaatag aaaagtggag acgaaatgct tctctttcga ttactgctgc aagacgaccg gacttgctaa aagatagata tggtgaaaat gacgtggaat aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIFNVLTIF PQMFPGPLGV SNLGSALKKG LWTLNVFDIR AFANNKHNTV DDTPYGGGPG MLLRADVLGR CIDEVLSLHP NTKLMFTSPR GVSFTQDIAR QTMNFDNITL LCGRFEGIDE RVVDFYKLQE VSIGDYVLSG GELAAMVIID TCVRMVPGVI GNAESLKQES MEGSLEYPQY TRPASWKGME VPEVLLTGNH GEIEKWRRNA SLSITAARRP DLLKDRYGEN DVE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcttcaac gttctaacaa tctttccgca aatgtttccc ggccctcttg gcgtatctaa tctgggttct gctctgaaaa aagggctgtg gacgttgaac gtttttgata ttagagcctt tgcgaataat aaacataata ctgttgatga taccccttat ggaggggggc caggcatgct tctcagggcc gatgtgttgg gaagatgcat agatgaagtt ttgtctctgc atccgaatac taaacttatg tttacttctc ctcgaggtgt aagttttact caagatatcg ctagacaaac tatgaatttt gacaatataa ccttactctg cgggcgcttt gaaggtattg atgagagagt ggtagatttt tacaaattgc aggaagttag cataggagat tatgttctct ctggtggtga gttagctgca atggtaatta tcgacacgtg tgttagaatg gtgccaggag ttataggtaa tgcagagagc ttaaagcagg aaagtatgga gggtagcctt gaataccccc agtatactag gccagcttcc tggaagggga tggaggttcc agaggtcctt cttactggga atcatggaga aatagaaaag tggagacgaa atgcttctct ttcgattact gctgcaagac gaccggactt gctaaaagat agatatggtg aaaatgacgt ggaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa