AnphA.00205.a

phosphoribosylaminoimidazole-succinocarboxamide synthase

CENTER ID: AnphA.00205.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00205.a.A1.GE26620 Full length( AnphA.00205.a ) 1 244
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00205.a.A1.PW27371 Full length( AnphA.00205.a ) 1 244

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.130
RefSeq: YP_504746.1
UniProt: Q2GLK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MENTREVLYE GKAKIILRHP ENERAVIQHF KDTLTAFNAQ RKSEIQGKGA LTNRISSLLM EHLEASGIRT HFLSFLNDKE QLVSKLNMIP LEVVVRNIAA GSMCKKFGIP KGKVLAEPIV EFYYKNDELS DPMIAEDHAT CFGLASKSEL ARMREIALQA NNILSALFSE VKILLVDFKL EFGKDSHSEQ EIVLGDEISP DTCRLWDTST LTVLDKDLYR FDIGNIEDGY KEVLQRLEQH FKNK
NT Sequence
ATGGAAAATA CACGAGAAGT TCTATATGAG GGTAAGGCCA AGATTATTCT GCGCCACCCG GAAAATGAAA GGGCGGTGAT ACAGCATTTT AAAGATACTC TTACAGCGTT CAATGCACAA AGAAAGAGTG AGATACAGGG AAAAGGTGCA TTAACTAACA GAATAAGTTC TCTTCTGATG GAACACCTTG AAGCGAGTGG CATACGTACA CACTTTTTGT CATTTTTGAA TGACAAAGAA CAGTTGGTCA GCAAGTTGAA CATGATTCCT CTAGAAGTGG TAGTTAGAAA TATCGCAGCT GGAAGCATGT GTAAAAAGTT CGGAATTCCT AAGGGTAAGG TTCTGGCTGA ACCCATAGTG GAGTTCTATT ATAAAAACGA CGAACTGTCA GACCCGATGA TAGCAGAGGA TCATGCAACT TGCTTTGGTC TTGCTTCAAA AAGCGAGCTC GCACGCATGC GGGAAATAGC ACTGCAAGCT AATAATATTC TGAGTGCACT CTTTTCCGAA GTTAAGATCT TACTCGTGGA TTTTAAGCTG GAATTTGGTA AAGATTCGCA TTCTGAGCAG GAAATTGTTC TAGGAGACGA AATTAGCCCT GATACTTGTA GGCTTTGGGA TACATCTACC CTTACGGTGC TGGATAAAGA TCTGTACAGG TTCGACATAG GCAACATAGA AGATGGGTAC AAAGAAGTTT TACAAAGGCT AGAACAGCAC TTTAAAAACA AA
Details for AnphA.00205.a.A1.GE26620
HARVESTED ON: 5/15/2009
SEQUENCED ON: 3/21/2012
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMENTREVLY EGKAKIILRH PENERAVIQH FKDTLTAFNA QRKSEIQGKG ALTNRISSLL MEHLEASGIR THFLSFLNDK EQLVSKLNMI PLEVVVRNIA AGSMCKKFGI PKGKVLAEPI VEFYYKNDEL SDPMIAEDHA TCFGLASKSE LARMREIALQ ANNILSALFS EVKILLVDFK LEFGKDSHSE QEIVLGDEIS PDTCRLWDTS TLTVLDKDLY RFDIGNIEDG YKEVLQRLEQ HFK
Validated NT Sequence
gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gaaaatacac gagaagttct atatgagggt aaggccaaga ttattctgcg ccacccggaa aatgaaaggg cggtgataca gcattttaaa gatactctta cagcgttcaa tgcacaaaga aagagtgaga tacagggaaa aggtgcatta actaacagaa taagttctct tctgatggaa caccttgaag cgagtggcat acgtacacac tttttgtcat ttttgaatga caaagaacag ttggtcagca agttgaacat gattcctcta gaagtggtag ttagaaatat cgcagctgga agcatgtgta aaaagttcgg aattcctaag ggtaaggttc tggctgaacc catagtggag ttctattata aaaacgacga actgtcagac ccgatgatag cagaggatca tgcaacttgc tttggtcttg cttcaaaaag cgagctcgca cgcatgcggg aaatagcact gcaagctaat aatattctga gtgcactctt ttccgaagtt aagatcttac tcgtggattt taagctggaa tttggtaaag attcgcattc tgagcaggaa attgttctag gagacgaaat tagccctgat acttgtaggc tttgggatac atctaccctt acggtgctgg ataaagatct gtacaggttc gacataggca acatagaaga tgggtacaaa gaagttttac aaaggctaga acagcacttt aaaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMENTREVLY EGKAKIILRH PENERAVIQH FKDTLTAFNA QRKSEIQGKG ALTNRISSLL MEHLEASGIR THFLSFLNDK EQLVSKLNMI PLEVVVRNIA AGSMCKKFGI PKGKVLAEPI VEFYYKNDEL SDPMIAEDHA TCFGLASKSE LARMREIALQ ANNILSALFS EVKILLVDFK LEFGKDSHSE QEIVLGDEIS PDTCRLWDTS TLTVLDKDLY RFDIGNIEDG YKEVLQRLEQ HFKNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaaataca cgagaagttc tatatgaggg taaggccaag attattctgc gccacccgga aaatgaaagg gcggtgatac agcattttaa agatactctt acagcgttca atgcacaaag aaagagtgag atacagggaa aaggtgcatt aactaacaga ataagttctc ttctgatgga acaccttgaa gcgagtggca tacgtacaca ctttttgtca tttttgaatg acaaagaaca gttggtcagc aagttgaaca tgattcctct agaagtggta gttagaaata tcgcagctgg aagcatgtgt aaaaagttcg gaattcctaa gggtaaggtt ctggctgaac ccatagtgga gttctattat aaaaacgacg aactgtcaga cccgatgata gcagaggatc atgcaacttg ctttggtctt gcttcaaaaa gcgagctcgc acgcatgcgg gaaatagcac tgcaagctaa taatattctg agtgcactct tttccgaagt taagatctta ctcgtggatt ttaagctgga atttggtaaa gattcgcatt ctgagcagga aattgttcta ggagacgaaa ttagccctga tacttgtagg ctttgggata catctaccct tacggtgctg gataaagatc tgtacaggtt cgacataggc aacatagaag atgggtacaa agaagtttta caaaggctag aacagcactt taaaaacaaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for AnphA.00205.a.A1.PW27371
PURIFICATION DATe: 7/7/2009
CONCENTRATION: 31.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 13
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMENTREVLY EGKAKIILRH PENERAVIQH FKDTLTAFNA QRKSEIQGKG ALTNRISSLL MEHLEASGIR THFLSFLNDK EQLVSKLNMI PLEVVVRNIA AGSMCKKFGI PKGKVLAEPI VEFYYKNDEL SDPMIAEDHA TCFGLASKSE LARMREIALQ ANNILSALFS EVKILLVDFK LEFGKDSHSE QEIVLGDEIS PDTCRLWDTS TLTVLDKDLY RFDIGNIEDG YKEVLQRLEQ HFK
Validated NT Sequence
gaaggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gaaaatacac gagaagttct atatgagggt aaggccaaga ttattctgcg ccacccggaa aatgaaaggg cggtgataca gcattttaaa gatactctta cagcgttcaa tgcacaaaga aagagtgaga tacagggaaa aggtgcatta actaacagaa taagttctct tctgatggaa caccttgaag cgagtggcat acgtacacac tttttgtcat ttttgaatga caaagaacag ttggtcagca agttgaacat gattcctcta gaagtggtag ttagaaatat cgcagctgga agcatgtgta aaaagttcgg aattcctaag ggtaaggttc tggctgaacc catagtggag ttctattata aaaacgacga actgtcagac ccgatgatag cagaggatca tgcaacttgc tttggtcttg cttcaaaaag cgagctcgca cgcatgcggg aaatagcact gcaagctaat aatattctga gtgcactctt ttccgaagtt aagatcttac tcgtggattt taagctggaa tttggtaaag attcgcattc tgagcaggaa attgttctag gagacgaaat tagccctgat acttgtaggc tttgggatac atctaccctt acggtgctgg ataaagatct gtacaggttc gacataggca acatagaaga tgggtacaaa gaagttttac aaaggctaga acagcacttt aaaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMENTREVLY EGKAKIILRH PENERAVIQH FKDTLTAFNA QRKSEIQGKG ALTNRISSLL MEHLEASGIR THFLSFLNDK EQLVSKLNMI PLEVVVRNIA AGSMCKKFGI PKGKVLAEPI VEFYYKNDEL SDPMIAEDHA TCFGLASKSE LARMREIALQ ANNILSALFS EVKILLVDFK LEFGKDSHSE QEIVLGDEIS PDTCRLWDTS TLTVLDKDLY RFDIGNIEDG YKEVLQRLEQ HFKNK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAAAATACA CGAGAAGTTC TATATGAGGG TAAGGCCAAG ATTATTCTGC GCCACCCGGA AAATGAAAGG GCGGTGATAC AGCATTTTAA AGATACTCTT ACAGCGTTCA ATGCACAAAG AAAGAGTGAG ATACAGGGAA AAGGTGCATT AACTAACAGA ATAAGTTCTC TTCTGATGGA ACACCTTGAA GCGAGTGGCA TACGTACACA CTTTTTGTCA TTTTTGAATG ACAAAGAACA GTTGGTCAGC AAGTTGAACA TGATTCCTCT AGAAGTGGTA GTTAGAAATA TCGCAGCTGG AAGCATGTGT AAAAAGTTCG GAATTCCTAA GGGTAAGGTT CTGGCTGAAC CCATAGTGGA GTTCTATTAT AAAAACGACG AACTGTCAGA CCCGATGATA GCAGAGGATC ATGCAACTTG CTTTGGTCTT GCTTCAAAAA GCGAGCTCGC ACGCATGCGG GAAATAGCAC TGCAAGCTAA TAATATTCTG AGTGCACTCT TTTCCGAAGT TAAGATCTTA CTCGTGGATT TTAAGCTGGA ATTTGGTAAA GATTCGCATT CTGAGCAGGA AATTGTTCTA GGAGACGAAA TTAGCCCTGA TACTTGTAGG CTTTGGGATA CATCTACCCT TACGGTGCTG GATAAAGATC TGTACAGGTT CGACATAGGC AACATAGAAG ATGGGTACAA AGAAGTTTTA CAAAGGCTAG AACAGCACTT TAAAAACAAA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA