AnphA.00202.a

phosphoglycerate kinase

CENTER ID: AnphA.00202.a
ORGANISM: Anaplasma phagocytophilum HZ
ASSOCIATED DISEASE: Human granulocytic anaplasmosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00202.a.B1.GE26906 Full length( AnphA.00202.a ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AnphA.00202.a.B1.PW26886 Full length( AnphA.00202.a ) 1 384

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|212042.8.peg.1414
RefSeq: YP_505830.1
UniProt: Q2GIH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGDIRDLPVI DAANISNSRV LLRVDLNVPI SNGEVHDKTR IERIAPTVEF LVKAKAKVVM ISHLGRPKAQ EEEFSLKQVV DVVSKVLGVE ITFIPDITRE EVDSVIDSLP WGSVVLLENL RFFTGEVEND LGFAGQLASL ADMYVNDAFS CSHRKHASVD AIMNLLPSFA GFNLQEELNY LDGIVSESPG AAIIGGAKTS VKIPMLRNFA SRLDFLILGG SLSTTFLDAM GFKVGNSFFE KNVEEALAVI EAAKESGCKI ILPVDHAVAQ NLEGPVTVKD NNDVTMEDSI FDIGEKTSRI IEETIRKCKT IFWNGPLGVF EHESFKGGTE RLTKALVSCH KENGAKTIIG GGDSIFAMRS FGYCEQDFSY VSTGGGALLH FLAL
NT Sequence
ATGGGAGATA TCCGGGATTT ACCAGTAATA GATGCTGCTA ATATAAGCAA CAGTCGGGTT CTGTTGCGTG TTGACCTCAA TGTTCCCATT AGCAATGGAG AGGTTCATGA TAAAACCAGA ATAGAGAGGA TTGCACCTAC AGTTGAGTTT CTGGTAAAGG CTAAGGCAAA GGTTGTTATG ATTTCCCACC TTGGAAGACC TAAGGCACAA GAAGAGGAGT TTTCTCTAAA GCAGGTAGTA GATGTTGTAA GCAAAGTCTT AGGAGTTGAA ATTACTTTCA TTCCCGATAT TACCAGAGAA GAAGTTGATA GCGTGATAGA TTCCCTACCT TGGGGCTCTG TAGTTTTGCT GGAAAACCTT AGATTTTTTA CTGGTGAAGT AGAGAACGAT TTAGGGTTTG CCGGGCAGCT CGCATCTCTG GCAGACATGT ATGTTAATGA TGCGTTTTCT TGCTCCCATA GAAAGCATGC TTCCGTAGAT GCCATTATGA ATCTACTGCC ATCATTTGCG GGGTTCAATC TACAAGAAGA ACTAAATTAC TTAGATGGTA TCGTGAGTGA AAGCCCCGGG GCTGCCATAA TAGGTGGAGC GAAAACTTCT GTAAAGATAC CTATGCTCCG AAATTTCGCA AGCAGGCTAG ATTTTCTAAT ATTGGGAGGA AGTTTATCCA CCACGTTTCT AGATGCCATG GGATTCAAGG TTGGAAATTC GTTTTTTGAG AAAAATGTTG AAGAAGCCTT GGCAGTTATA GAAGCTGCGA AAGAGAGTGG TTGTAAGATC ATATTACCTG TAGATCACGC AGTCGCGCAA AACCTAGAAG GTCCAGTTAC GGTAAAAGAT AATAACGATG TAACAATGGA AGACTCAATA TTTGATATTG GGGAGAAAAC TTCCCGCATC ATAGAAGAAA CCATCAGGAA GTGTAAAACC ATATTTTGGA ATGGGCCATT AGGTGTTTTC GAGCATGAGT CTTTTAAGGG AGGAACTGAG AGATTAACCA AAGCACTCGT ATCTTGCCAT AAGGAAAACG GAGCTAAAAC TATCATAGGG GGCGGCGATA GTATATTCGC TATGCGATCG TTTGGTTATT GTGAACAAGA TTTTTCATAT GTCTCTACTG GCGGAGGAGC ACTTCTGCAC TTCCTTGCAT TG
Details for AnphA.00202.a.B1.GE26906
HARVESTED ON: 6/18/2009
SEQUENCED ON: 7/9/2009
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHPMG DIRDLPVIDA ANISNSRVLL RVDLNVPISN GEVHDKTRIE RIAPTVEFLV KAKAKVVMIS HLGRPKAQEE EFSLKQVVDV VSKVLGVEIT FIPDITREEV DSVIDSLPWG SVVLLENLRF FTGEVENDLG FAGQLASLAD MYVNDAFSCS HRKHASVDAI MNLLPSFAGF NLQEELNYLD GIVSESPGAA IIGGAKTSVK IPMLRNFASR LDFLILGGSL STTFLDAMGF KVGNSFFEKN VEEALAVIEA AKESGCKIIL PVDHAVAQNL EGPVTVKDNN DVTMEDSIFD IGEKTSRIIE ETIRKCKTIF WNGPLGVFEH ESFKGGTERL TKALVSCHKE NGAKTIIGGG DSIFAMRSFG YCEQDFSYVS TGGGALLHFL AL
Validated NT Sequence
atggctcacc accaccacca ccctatggga gatatccggg atttaccagt aatagatgct gctaatataa gcaacagtcg ggttctgttg cgtgttgacc tcaatgttcc cattagcaat ggagaggttc atgataaaac cagaatagag aggattgcac ctacagttga gtttctggta aaggctaagg caaaggttgt tatgatttcc caccttggaa gacctaaggc acaagaagag gagttttctc taaagcaggt agtagatgtt gtaagcaaag tcttaggagt tgaaattact ttcattcccg atattaccag agaagaagtt gatagcgtga tagattccct accttggggc tctgtagttt tgctggaaaa ccttagattt tttactggtg aagtagagaa cgatttaggg tttgccgggc agctcgcatc tctggcagac atgtatgtta atgatgcgtt ttcttgctcc catagaaagc atgcttccgt agatgccatt atgaatctac tgccatcatt tgcggggttc aatctacaag aagaactaaa ttacttagat ggtatcgtga gtgaaagccc cggggctgcc ataataggtg gagcgaaaac ttctgtaaag atacctatgc tccgaaattt cgcaagcagg ctagattttc taatattggg aggaagttta tccaccacgt ttctagatgc catgggattc aaggttggaa attcgttttt tgagaaaaat gttgaagaag ccttggcagt tatagaagct gcgaaagaga gtggttgtaa gatcatatta cctgtagatc acgcagtcgc gcaaaaccta gaaggtccag ttacggtaaa agataataac gatgtaacaa tggaagactc aatatttgat attggggaga aaacttcccg catcatagaa gaaaccatca ggaagtgtaa aaccatattt tggaatgggc cattaggtgt tttcgagcat gagtctttta agggaggaac tgagagatta accaaagcac tcgtatcttg ccataaggaa aacggagcta aaactatcat agggggcggc gatagtatat tcgctatgcg atcgtttggt tattgtgaac aagatttttc atatgtctct actggcggag gagcacttct gcacttcctt gcattgtaag tgagtaagat
Expected Protein Sequence
MAHHHHHHMG DIRDLPVIDA ANISNSRVLL RVDLNVPISN GEVHDKTRIE RIAPTVEFLV KAKAKVVMIS HLGRPKAQEE EFSLKQVVDV VSKVLGVEIT FIPDITREEV DSVIDSLPWG SVVLLENLRF FTGEVENDLG FAGQLASLAD MYVNDAFSCS HRKHASVDAI MNLLPSFAGF NLQEELNYLD GIVSESPGAA IIGGAKTSVK IPMLRNFASR LDFLILGGSL STTFLDAMGF KVGNSFFEKN VEEALAVIEA AKESGCKIIL PVDHAVAQNL EGPVTVKDNN DVTMEDSIFD IGEKTSRIIE ETIRKCKTIF WNGPLGVFEH ESFKGGTERL TKALVSCHKE NGAKTIIGGG DSIFAMRSFG YCEQDFSYVS TGGGALLHFL AL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggag atatccggga tttaccagta atagatgctg ctaatataag caacagtcgg gttctgttgc gtgttgacct caatgttccc attagcaatg gagaggttca tgataaaacc agaatagaga ggattgcacc tacagttgag tttctggtaa aggctaaggc aaaggttgtt atgatttccc accttggaag acctaaggca caagaagagg agttttctct aaagcaggta gtagatgttg taagcaaagt cttaggagtt gaaattactt tcattcccga tattaccaga gaagaagttg atagcgtgat agattcccta ccttggggct ctgtagtttt gctggaaaac cttagatttt ttactggtga agtagagaac gatttagggt ttgccgggca gctcgcatct ctggcagaca tgtatgttaa tgatgcgttt tcttgctccc atagaaagca tgcttccgta gatgccatta tgaatctact gccatcattt gcggggttca atctacaaga agaactaaat tacttagatg gtatcgtgag tgaaagcccc ggggctgcca taataggtgg agcgaaaact tctgtaaaga tacctatgct ccgaaatttc gcaagcaggc tagattttct aatattggga ggaagtttat ccaccacgtt tctagatgcc atgggattca aggttggaaa ttcgtttttt gagaaaaatg ttgaagaagc cttggcagtt atagaagctg cgaaagagag tggttgtaag atcatattac ctgtagatca cgcagtcgcg caaaacctag aaggtccagt tacggtaaaa gataataacg atgtaacaat ggaagactca atatttgata ttggggagaa aacttcccgc atcatagaag aaaccatcag gaagtgtaaa accatatttt ggaatgggcc attaggtgtt ttcgagcatg agtcttttaa gggaggaact gagagattaa ccaaagcact cgtatcttgc cataaggaaa acggagctaa aactatcata gggggcggcg atagtatatt cgctatgcga tcgtttggtt attgtgaaca agatttttca tatgtctcta ctggcggagg agcacttctg cacttccttg cattgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for AnphA.00202.a.B1.PW26886
PURIFICATION DATe: 8/3/2009
CONCENTRATION: 19.79mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHPMG DIRDLPVIDA ANISNSRVLL RVDLNVPISN GEVHDKTRIE RIAPTVEFLV KAKAKVVMIS HLGRPKAQEE EFSLKQVVDV VSKVLGVEIT FIPDITREEV DSVIDSLPWG SVVLLENLRF FTGEVENDLG FAGQLASLAD MYVNDAFSCS HRKHASVDAI MNLLPSFAGF NLQEELNYLD GIVSESPGAA IIGGAKTSVK IPMLRNFASR LDFLILGGSL STTFLDAMGF KVGNSFFEKN VEEALAVIEA AKESGCKIIL PVDHAVAQNL EGPVTVKDNN DVTMEDSIFD IGEKTSRIIE ETIRKCKTIF WNGPLGVFEH ESFKGGTERL TKALVSCHKE NGAKTIIGGG DSIFAMRSFG YCEQDFSYVS TGGGALLHFL AL
Validated NT Sequence
atggctcacc accaccacca ccctatggga gatatccggg atttaccagt aatagatgct gctaatataa gcaacagtcg ggttctgttg cgtgttgacc tcaatgttcc cattagcaat ggagaggttc atgataaaac cagaatagag aggattgcac ctacagttga gtttctggta aaggctaagg caaaggttgt tatgatttcc caccttggaa gacctaaggc acaagaagag gagttttctc taaagcaggt agtagatgtt gtaagcaaag tcttaggagt tgaaattact ttcattcccg atattaccag agaagaagtt gatagcgtga tagattccct accttggggc tctgtagttt tgctggaaaa ccttagattt tttactggtg aagtagagaa cgatttaggg tttgccgggc agctcgcatc tctggcagac atgtatgtta atgatgcgtt ttcttgctcc catagaaagc atgcttccgt agatgccatt atgaatctac tgccatcatt tgcggggttc aatctacaag aagaactaaa ttacttagat ggtatcgtga gtgaaagccc cggggctgcc ataataggtg gagcgaaaac ttctgtaaag atacctatgc tccgaaattt cgcaagcagg ctagattttc taatattggg aggaagttta tccaccacgt ttctagatgc catgggattc aaggttggaa attcgttttt tgagaaaaat gttgaagaag ccttggcagt tatagaagct gcgaaagaga gtggttgtaa gatcatatta cctgtagatc acgcagtcgc gcaaaaccta gaaggtccag ttacggtaaa agataataac gatgtaacaa tggaagactc aatatttgat attggggaga aaacttcccg catcatagaa gaaaccatca ggaagtgtaa aaccatattt tggaatgggc cattaggtgt tttcgagcat gagtctttta agggaggaac tgagagatta accaaagcac tcgtatcttg ccataaggaa aacggagcta aaactatcat agggggcggc gatagtatat tcgctatgcg atcgtttggt tattgtgaac aagatttttc atatgtctct actggcggag gagcacttct gcacttcctt gcattgtaag tgagtaagat
Expressed Protein Sequence
MAHHHHHHMG DIRDLPVIDA ANISNSRVLL RVDLNVPISN GEVHDKTRIE RIAPTVEFLV KAKAKVVMIS HLGRPKAQEE EFSLKQVVDV VSKVLGVEIT FIPDITREEV DSVIDSLPWG SVVLLENLRF FTGEVENDLG FAGQLASLAD MYVNDAFSCS HRKHASVDAI MNLLPSFAGF NLQEELNYLD GIVSESPGAA IIGGAKTSVK IPMLRNFASR LDFLILGGSL STTFLDAMGF KVGNSFFEKN VEEALAVIEA AKESGCKIIL PVDHAVAQNL EGPVTVKDNN DVTMEDSIFD IGEKTSRIIE ETIRKCKTIF WNGPLGVFEH ESFKGGTERL TKALVSCHKE NGAKTIIGGG DSIFAMRSFG YCEQDFSYVS TGGGALLHFL AL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAG ATATCCGGGA TTTACCAGTA ATAGATGCTG CTAATATAAG CAACAGTCGG GTTCTGTTGC GTGTTGACCT CAATGTTCCC ATTAGCAATG GAGAGGTTCA TGATAAAACC AGAATAGAGA GGATTGCACC TACAGTTGAG TTTCTGGTAA AGGCTAAGGC AAAGGTTGTT ATGATTTCCC ACCTTGGAAG ACCTAAGGCA CAAGAAGAGG AGTTTTCTCT AAAGCAGGTA GTAGATGTTG TAAGCAAAGT CTTAGGAGTT GAAATTACTT TCATTCCCGA TATTACCAGA GAAGAAGTTG ATAGCGTGAT AGATTCCCTA CCTTGGGGCT CTGTAGTTTT GCTGGAAAAC CTTAGATTTT TTACTGGTGA AGTAGAGAAC GATTTAGGGT TTGCCGGGCA GCTCGCATCT CTGGCAGACA TGTATGTTAA TGATGCGTTT TCTTGCTCCC ATAGAAAGCA TGCTTCCGTA GATGCCATTA TGAATCTACT GCCATCATTT GCGGGGTTCA ATCTACAAGA AGAACTAAAT TACTTAGATG GTATCGTGAG TGAAAGCCCC GGGGCTGCCA TAATAGGTGG AGCGAAAACT TCTGTAAAGA TACCTATGCT CCGAAATTTC GCAAGCAGGC TAGATTTTCT AATATTGGGA GGAAGTTTAT CCACCACGTT TCTAGATGCC ATGGGATTCA AGGTTGGAAA TTCGTTTTTT GAGAAAAATG TTGAAGAAGC CTTGGCAGTT ATAGAAGCTG CGAAAGAGAG TGGTTGTAAG ATCATATTAC CTGTAGATCA CGCAGTCGCG CAAAACCTAG AAGGTCCAGT TACGGTAAAA GATAATAACG ATGTAACAAT GGAAGACTCA ATATTTGATA TTGGGGAGAA AACTTCCCGC ATCATAGAAG AAACCATCAG GAAGTGTAAA ACCATATTTT GGAATGGGCC ATTAGGTGTT TTCGAGCATG AGTCTTTTAA GGGAGGAACT GAGAGATTAA CCAAAGCACT CGTATCTTGC CATAAGGAAA ACGGAGCTAA AACTATCATA GGGGGCGGCG ATAGTATATT CGCTATGCGA TCGTTTGGTT ATTGTGAACA AGATTTTTCA TATGTCTCTA CTGGCGGAGG AGCACTTCTG CACTTCCTTG CATTGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT