SSGCID: Target Page

AnmaA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID:	AnmaA.17987.a
ORGANISM:	Anaplasma marginale
ASSOCIATED DISEASE:	Bovine anaplasmosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AnmaA.17987.a.B1.GE41311	full length		1	379

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320483.3.peg.883
UniProt:	B9KGQ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVLMDIAVGG GLECSISRED LLQSLAYVSS VIERRNSIAV LSCVRVEAKD GVVELSSTDL DISIEACVNA SVVHKGVVVA SAQLLYDIVR KLSGDCAVGF KMSDGRLLVE CGNAKFFLHV VAPEKFPAIE GGEVDCGFDM QVSHIAELLN KTKFSMSVEE SRYNLRGVYM HVAEDMLCCV ATDGHRLSLA KVNRPEGLQP DIGVIVPRKT VNELLKIIGG GGDASQVVSV SISQRKICFR YKGYFMVSKV VDGVFPDYLS IIPQERGASV SMDACSLLSA VDRVSVVVFD KVKAIKLSFA SGRLTLSSAS SDQGDAREEL EVDYKGDEVT IGLNARYLIE VLGCMKGACE VVFQDGNTAV LIQDKEPGGS MHIIMPMRI

NT Sequence

atggtgctta tggatatcgc agtgggtggt gggttggaat gcagtataag tagggaggat ttgctgcagt ccctggccta cgtcagcagc gtgatagaga ggcgcaacag catagctgtg ttgtcatgcg tcagggtgga ggccaaagat ggtgttgttg agctgtcttc gacggacctt gatatttcaa tagaagcgtg cgtcaacgct agcgttgttc ataagggagt cgtagtcgca tctgctcagt tgttgtatga cattgtcagg aagctttcgg gtgattgcgc agttggcttc aagatgagcg atgggcggct gctcgttgaa tgcggaaatg ccaagttctt tctccatgtt gtggcgccgg aaaagttccc cgcaattgag ggtggcgagg ttgactgcgg ttttgacatg caggtttccc atattgccga gttgctcaac aagacaaaat tctccatgtc cgttgaggag agtaggtata acctcagggg tgtctacatg catgttgcgg aggatatgtt gtgctgtgtg gcgaccgatg ggcaccgctt gtcgcttgcg aaggtgaata ggccggaagg cctgcagcct gatatcggcg tgattgtacc taggaagacg gtaaatgagc ttttgaaaat aataggtggc ggtggggatg cgtcacaagt tgtatctgta agcatttcac agcgcaagat atgcttcagg tacaagggat atttcatggt ttccaaagtg gttgacgggg tgtttccgga ctacttatct ataattccgc aagagagggg tgcgagtgta tctatggatg cgtgctctct tcttagcgct gtggatcggg tttctgtggt agtttttgat aaggtgaagg ccattaagct gtcattcgcc tccggtcggc tgactctcag ctccgcgtcg tccgatcagg gtgatgccag ggaggagctt gaggtagatt acaaaggtga tgaagtcact attgggctca atgccaggta tcttattgag gtgctgggtt gcatgaaggg ggcctgtgag gtcgtatttc aggatggtaa cactgccgtg ctgattcagg acaaggaacc cggcggctcc atgcacataa taatgcctat gagaatatag

Details for AnmaA.17987.a.B1.GE41311

HARVESTED ON:	3/31/2017
SEQUENCED ON:	4/10/2017
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMV LMDIAVGGGL ECSISREDLL QSLAYVSSVI ERRNSIAVLS CVRVEAKDGV VELSSTDLDI SIEACVNASV VHKGVVVASA QLLYDIVRKL SGDCAVGFKM SDGRLLVECG NAKFFLHVVA PEKFPAIEGG EVDCGFDMQV SHIAELLNKT KFSMSVEESR YNLRGVYMHV AEDMLCCVAT DGHRLSLAKV NRPEGLQPDI GVIVPRKTVN ELLKIIGGGG DASQVVSVSI SQRKICFRYK GYFMVSKVVD GVFPDYLSII PQERGASVSM DACSLLSAVD RVSVVVFDKV KAIKLSFASG RLTLSSASSD QGDAREELEV DYKGDEVTIG LNARYLIEVL GCMKGACEVV FQDGNTAVLI QDKEPGGSMH

Validated NT Sequence

tgtgcatgga gccgccgggt tccttgtcct gaatcagcac ggcagtgtta ccatcctgaa atacgacctc acaggccccc ttcatgcaac ccagcacctc aataagatac ctggcattga gcccaatagt gacttcatca cctttgtaat ctacctcaag ctcctccctg gcatcaccct gatcggacga cgcggagctg agagtcagcc gaccggaggc gaatgacagc ttaatggcct tcaccttatc aaaaactacc acagaaaccc gatccacagc gctaagaaga gagcacgcat ccatagatac actcgcaccc ctctcttgcg gaattataga taagtagtcc ggaaataccc cgtcaaccac tttggaaacc atgaaatatc ccttgtacct gaagcatatt ttgcgctgtg aaatgcttac agatacaact tgtgacgcat ccccgccgcc acctattatt ttcaaaagct catttactgt cttcctaggt acaatcacgc cgatatcagg ctgcaggcct tccggcctat tcaccttcgc aagcgacaag cggtgcccat cggtcgccac gcagcacaac atatcctccg caacatgcat gtagacaccc ctgaggttat acctactctc ctcaacggac atggagaatt ttgtcttgtt gagcaactcg gcaatatggg aaacctgcat gtcaaaaccg cagtcaacct cgccaccctc aattgcgggg aacttctccg gtgccacaac atggagaaag aacttggcat ttccgcattc aacgagcagc cgcccatcgc tcatcttgaa gccaactgcg caatcacccg aaagcttcct gacaatgtca tacaacaact gagcagatgc gactacgact cccttatgaa caacgctagc gttgacgcac gcttctattg aaatatcaag gtccgtcgaa gacagctcaa caacaccatc tttggcctcc accctgacgc atgacaacac agctatgctg ttgcgcctct ctatcacgct gctgacgtag gccagggact gcagcaaatc ctccctactt atactgcatt ccaacccacc acccactgcg atatccataa gcaccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtgc ttatggatat cgcagtgggt ggtgggttgg aatgcagtat aagtagggag gatttgctgc agtccctggc ctacgtcagc agcgtgatag agaggcgcaa cagcatagct gtgttgtcat gcgtcagggt ggaggccaaa gatggtgttg ttgagctgtc ttcgacggac cttgatattt caatagaagc gtgcgtcaac gctagcgttg ttcataaggg agtcgtagtc gcatctgctc agttgttgta tgacattgtc aggaagcttt cgggtgattg cgcagttggc ttcaagatga gcgatgggcg gctgctcgtt gaatgcggaa atgccaagtt ctttctccat gttgtggcgc cggaaaagtt ccccgcaatt gagggtggcg aggttgactg cggttttgac atgcaggttt cccatattgc cgagttgctc aacaagacaa aattctccat gtccgttgag gagagtaggt ataacctcag gggtgtctac atgcatgttg cggaggatat gttgtgctgt gtggcgaccg atgggcaccg cttgtcgctt gcgaaggtga ataggccgga aggcctgcag cctgatatcg gcgtgattgt acctaggaag acggtaaatg agcttttgaa aataataggt ggcggtgggg atgcgtcaca agttgtatct gtaagcattt cacagcgcaa gatatgcttc aggtacaagg gatatttcat ggtttccaaa gtggttgacg gggtgtttcc ggactactta tctataattc cgcaagagag gggtgcgagt gtatctatgg atgcgtgctc tcttcttagc gctgtggatc gggtttctgt ggtagttttt gataaggtga aggccattaa gctgtcattc gcctccggtc ggctgactct cagctccgcg tcgtccgatc agggtgatgc cagggaggag cttgaggtag attacaaagg tgatgaagtc actattgggc tcaatgccag gtatcttatt gaggtgctgg gttgcatgaa gggggcctgt gaggtcgtat ttcaggatgg taacactgcc gtgctgattc aggacaagga acccggcggc tccatgcaca taataatgcc tatgagaata tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat