AlveB.17791.a

Structural polyprotein; p130

CENTER ID: AlveB.17791.a
ORGANISM: Alphavirus Chikungunya virus Senegal 37997
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AlveB.17791.a.D16.GE00389 full length 326 749
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|37124.1796.CDS.1
UniProt: Q5XXP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEFIPTQTFY NRRYQPRPWA PRPTIQVIRP RPRPQRQAGQ LAQLISAVNK LTMRAVPQQK PRRNRKNKKQ RQKKQAPQND PKQKKQPPQK KPAQKKKKPG RRERMCMKIE NDCIFEVKHE GKVMGYACLV GDKVMKPAHV KGTIDNADLA KLAFKRSSKY DLECAQIPVH MKSDASKFTH EKPEGYYNWH HGAVQYSGGR FTIPTGAGKP GDSGRPIFDN KGRVVAIVLG GANEGARTAL SVVTWNKDIV TKITPEGAEE WSLALPVLCL LANTTFPCSQ PPCTPCCYEK EPESTLRMLE DNVMRPGYYQ LLKASLTCSP HRQRRSTKDN FNVYKATRPY LAHCPDCGEG HSCHSPIALE RIRNEATDGT LKIQVSLQIG IKTDDSHDWT KLRYMDSHTP ADAERAGLLV RTSAPCTITG TMGHFILARC PKGETLTVGF TDSRKISHTC THPFHHEPPV IGRERFHSRP QHGKELPCST YVQSTAATAE EIEVHMPPDT PDRTLMTQQS GNVKITVNGQ TVRYKCNCGG SNEGLTTTDK VINNCKIDQC HAAVTNHKNW QYNSPLVPRN AELGDRKGKI HIPFPLANVT CRVPKARNPT VTYGKNQVTM LLYPDHPTLL SYRNMGQEPN YHEEWVTHKK EVTLTVPTEG LEVTWGNNEP YKYWPQMSTN GTAHGHPHEI ILYYYELYPT MTVVIVSVAS FVLLSMVGTA VGMCVCARRR CITPYELTPG ATVPFLLSLL CCVRTTKAAT YYEAAAYLWN EQQPLFWLQA LIPLAALIVL CNCLKLLPCC CKTLAFLAVM SIGAHTVSAY EHVTVIPNTV GVPYKTLVNR PGYSPMVLEM ELQSVTLEPT LSLDYITCEY KTVIPSPYVK CCGTAECKDK SLPDYSCKVF TGVYPFMWGG AYCFCDAENT QLSEAHVEKS ESCKTEFASA YRAHTASASA KLRVLYQGNN ITVAAYANGD HAVTVKDAKF VVGPMSSAWT PFDNKIVVYK GDVYNMDYPP FGAGRPGQFG DIQSRTPESK DVYANTQLVL QRPAAGTVHV PYSQAPSGFK YWLKERGASL QHTAPFGCQI ATNPVRAVNC AVGNIPISID IPDAAFTRVV DAPSVTDMSC EVPACTHSSD FGGVAIIKYT ASKKGKCAVH SMTNAVTIRE ADVEVEGNSQ LQISFSTALA SAEFRVQVCS TQVHCAAACH PPKDHIVNYP ASHTTLGVQD ISTTAMSWVQ KITGGVGLIV AVAALILIVV LCVSFSRH
NT Sequence
atggagttca tcccgacgca aactttctat aacagaaggt accaaccccg accctgggcc ccacgcccta caattcaagt aattagacct agaccacgtc cacagaggca ggctgggcaa ctcgcccagc tgatctccgc agtcaacaaa ttgaccatgc gcgcggtacc tcaacagaag cctcgcagaa atcggaaaaa caagaagcaa aggcagaaga agcaggcgcc gcaaaacgac ccaaagcaaa agaagcaacc accacaaaag aagccggctc aaaagaagaa gaaaccaggc cgtagggaga gaatgtgcat gaaaattgaa aatgattgca tcttcgaagt caagcatgaa ggcaaagtga tgggctacgc atgcctggtg ggggataaag taatgaaacc agcacatgtg aagggaacta tcgacaatgc cgatctggct aaactggcct ttaagcggtc gtctaaatac gatcttgaat gtgcacagat accggtgcac atgaagtctg atgcctcgaa gtttacccac gagaaacccg aggggtacta taactggcat cacggagcag tgcagtattc aggaggccgg ttcactatcc cgacgggtgc aggcaagccg ggagacagcg gcagaccgat cttcgacaac aaaggacggg tggtggccat cgtcctagga ggggccaacg aaggtgcccg cacggccctc tccgtggtga cgtggaacaa agacatcgtc acaaaaatta cccctgaggg agccgaagag tggagcctcg ccctcccggt cttgtgcctg ttggcaaaca ctacattccc ctgctctcag ccgccttgca caccctgctg ctacgaaaag gaaccggaaa gcaccttgcg catgcttgag gacaacgtga tgagacccgg atactaccag ctactaaaag catcgctgac ttgctctccc caccgccaaa gacgcagtac taaggacaat tttaatgtct ataaagccac aagaccatat ctagctcatt gtcctgactg cggagaaggg cattcgtgcc acagccctat cgcattggag cgcatcagaa atgaagcaac ggacggaacg ctgaaaatcc aggtctcttt gcagatcggg ataaagacag atgacagcca cgattggacc aagctgcgct atatggatag ccatacgcca gcggacgcgg agcgagccgg attgcttgta aggacttcag caccgtgcac gatcaccggg accatgggac actttattct cgcccgatgc ccgaaaggag agacgctgac agtgggattt acggacagca gaaagatcag ccacacatgc acacacccgt tccatcatga accacctgtg ataggtaggg agaggttcca ctctcgacca caacatggta aagagttacc ttgcagcacg tacgtgcaga gcaccgctgc cactgctgag gagatagagg tgcatatgcc cccagatact cctgaccgca cgctgatgac gcagcagtct ggcaacgtga agatcacagt taatgggcag acggtgcggt acaagtgcaa ctgcggtggc tcaaacgagg gactgacaac cacagacaaa gtgatcaata actgcaaaat tgatcagtgc catgctgcag tcactaatca caagaattgg caatacaact cccctttagt cccgcgcaac gctgaactcg gggaccgtaa aggaaagatc cacatcccat tcccattggc aaacgtgact tgcagagtgc caaaagcaag aaaccctaca gtaacttacg gaaaaaacca agtcaccatg ctgctgtatc ctgaccatcc gacactcttg tcttaccgta acatgggaca ggaaccaaat taccacgagg agtgggtgac acacaagaag gaggttacct tgaccgtgcc tactgagggt ctggaggtca cttggggcaa caacgaacca tacaagtact ggccgcagat gtctacgaac ggtactgctc atggtcaccc acatgagata atcttgtact attatgagct gtaccccact atgactgtag tcattgtgtc ggtggcctcg ttcgtgcttc tgtcgatggt gggcacagca gtgggaatgt gtgtgtgcgc acggcgcaga tgcattacac catatgaatt aacaccagga gccactgttc ccttcctgct cagcctgcta tgctgcgtca gaacgaccaa ggcggccaca tattacgagg ctgcggcata tctatggaac gaacagcagc ccctgttctg gttgcaggct cttatcccgc tggccgcctt gatcgtcctg tgcaactgtc tgaaactctt gccatgctgc tgtaagaccc tggctttttt agccgtaatg agcatcggtg cccacactgt gagcgcgtac gaacacgtaa cagtgatccc gaacacggtg ggagtaccgt ataagactct tgtcaacaga ccgggttaca gccccatggt gttggagatg gagctacaat cagtcacctt ggaaccaaca ctgtcacttg actacatcac gtgcgagtac aaaactgtca tcccctcccc gtacgtgaag tgctgtggta cagcagagtg caaggacaag agcctaccag actacagctg caaggtcttt actggagtct acccatttat gtggggcggc gcctactgct tttgcgacgc cgaaaatacg caattgagcg aggcacatgt agagaaatct gaatcttgca aaacagagtt tgcatcggcc tacagagccc acaccgcatc ggcgtcggcg aagctccgcg tcctttacca aggaaacaac attaccgtag ctgcctacgc taacggtgac catgccgtca cagtaaagga cgccaagttt gtcgtgggcc caatgtcctc cgcctggaca ccttttgaca acaaaatcgt ggtgtacaaa ggcgacgtct acaacatgga ctacccacct tttggcgcag gaagaccagg acaatttggt gacattcaaa gtcgtacacc ggaaagtaaa gacgtttatg ccaacactca gttggtacta cagaggccag cagcaggcac ggtacatgta ccatactctc aggcaccatc tggcttcaag tattggctga aggaacgagg agcatcgcta cagcacacgg caccgttcgg ttgccagatt gcgacaaacc cggtaagagc tgtaaattgc gctgtgggga acataccaat ttccatcgac ataccggatg cggcctttac tagggttgtc gatgcaccct ctgtaacgga catgtcatgc gaagtaccag cctgcactca ctcctccgac tttgggggcg tcgccatcat caaatacaca gctagcaaga aaggtaaatg tgcagtacat tcgatgacca acgccgttac cattcgagaa gccgacgtag aagtagaggg gaactcccag ctgcaaatat ccttctcaac agccctggca agcgccgagt ttcgcgtgca agtgtgctcc acacaagtac actgcgcagc cgcatgccac cctccaaagg accacatagt caattaccca gcatcacaca ccacccttgg ggtccaggat atatccacaa cggcaatgtc ttgggtgcag aagattacgg gaggagtagg attaattgtt gctgttgctg ccttaatttt aattgtggtg ctatgcgtgt cgtttagcag gcactaa
Details for AlveB.17791.a.D16.GE00389
HARVESTED ON: data unavailable
SEQUENCED ON: 5/4/2017
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSSTK DNFNVYKATR PYLAHCPDCG EGHSCHSPIA LERIRNEATD GTLKIQVSLQ IGIKTDDSHD WTKLRYMDSH TPADAERAGL LVRTSAPCTI TGTMGHFILA RCPKGETLTV GFTDSRKISH TCTHPFHHEP PVIGRERFHS RPQHGKELPC STYVQSTAAT AEEIEVHMPP DTPDRTLMTQ QSGNVKITVN GQTVRYKCNC GGSNEGLTTT DKVINNCKID QCHAAVTNHK NWQYNSPLVP RNAELGDRKG KIHIPFPLAN VTCRVPKARN PTVTYGKNQV TMLLYPDHPT LLSYRNMGQE PNYHEEWVTH KKEVTLTVPT EGLEVTWGNN EPYKYWPQMS TNGTAHGHPH EIILYYYELY PTMTVVIVSV ASFVLLSMVG TAVGMCVCAR RRCITPYELT PGATVPFLLS LLCCVRTTKA
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggatc cagcaccaaa gacaacttta atgtctataa ggcaacccgt ccatatctgg cgcattgtcc ggactgcggc gagggtcatt cgtgtcacag cccgattgcg ctggagcgta tccgcaacga agcgaccgac ggcacgctga aaattcaggt tagcctgcag atcggtatca agaccgatga cagccacgac tggacgaaat tgcgctacat ggacagccat accccggcag atgcggagcg tgctggcttg ttggtccgta ccagcgcacc gtgcaccatc accggtacga tgggccactt cattctggcg cgttgtccga agggtgagac tctgacggtt ggttttaccg atagccgcaa aatcagccac acgtgtaccc atccgtttca tcacgagcca ccggtcatcg gtcgcgagcg cttccatagc cgtccgcagc acggcaaaga actgccgtgc agcacctacg ttcagtccac tgccgcaacc gcggaagaga ttgaagtgca catgcctccg gacaccccgg atcgtaccct gatgactcag caatctggta acgtcaagat caccgtgaac ggccagactg ttcgttataa gtgcaattgt ggtggcagca atgagggtct gacgacgacg gataaagtga ttaacaattg caagattgac caatgccacg cggctgttac gaaccacaaa aactggcaat acaactcccc gctggtgccg cgtaacgccg agctgggcga tcgcaagggt aagatccaca ttccgttccc gctggccaat gttacctgtc gtgttccgaa agcgcgtaat cctaccgtca cgtacggtaa gaaccaagtg acgatgctgc tgtatccaga tcatccgacc ctgctgagct atagaaatat gggtcaagag ccgaattacc acgaagagtg ggtgacgcac aaaaaagaag taaccctgac cgttccgacc gagggcttag aggtgacctg gggcaataat gaaccttata agtactggcc gcagatgagc accaacggca cggcacatgg tcacccgcac gaaatcattc tgtactatta cgaactgtac ccgaccatga ccgtcgtcat tgtgagcgtg gcatcctttg ttctgctgtc tatggtcggt accgcggtcg gtatgtgcgt ttgcgctcgt cgtcgctgca tcacgccgta cgaactgacg ccgggtgcga ccgtgccgtt cttgctgagc ctgctgtgct gtgttcgcac tacgaaagcc tgataagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at