SSGCID: Target Page

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	AA START	AA STOP
AlveA.17791.a.A21.GE33830	Capsid	1	275
AlveA.17791.a.A22.GE33831	Capsid	1	267
AlveA.17791.a.A23.GE33832	Capsid	13	275
AlveA.17791.a.A24.GE33833	Capsid	13	267
AlveA.17791.a.A25.GE33834	Capsid	118	275
AlveA.17791.a.A26.GE33835	Capsid	118	267
AlveA.17791.a.A41.GE33836	E2	348	751
AlveA.17791.a.A42.GE33837	E2	348	732
AlveA.17791.a.A43.GE33838	E2	348	743
AlveA.17791.a.A51.GE33842	E1 truncated (815-1226)	815	1226
AlveA.17791.a.A52.GE33843	E1 truncated (815-1226)	815	1151
AlveA.17791.a.A53.GE33844	E1 truncated (815-1226)	815	1209

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AlveA.17791.a.A26.PW34445	Capsid		118	267

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|11036.192.CDS.2
UniProt:	P09592
ViPR:	link
ViPR:	link
ViPR:	link
ViPR:	link
ViPR:	link

Details for AlveA.17791.a.A21.GE33830

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	33kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMFPFQPMYP MQPMPYRNPF AAPRRPWFPR TDPFLAMQVQ ELTRSMANLT FKQRRDAPPE GPSAKKPKKE ASQKQKGGGQ GKKKKNQGKK KAKTGPPNPK AQNGNKKKTN KKPGKRQRMV MKLESDKTFP IMLEGKINGY ACVVGGKLFR PMHVEGKIDN DVLAALKTKK ASKYDLEYAD VPQNMRADTF KYTHEKPQGY YSWHHGAVQY ENGRFTVPKG VGAKGDSGRP ILDNQGRVVA IVLGGVNEGS RTALSVVMWN EKGVTVKYTP ENCEQWX

Validated NT Sequence

attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtttc cgttccagcc gatgtacccg atgcaaccga tgccgtatag aaatccgttc gctgctccgc gtcgtccgtg gtttcctcgt accgacccat tcttagctat gcaggttcag gaactgacta gatccatggc taacctgacc tttaaacagc gccgtgatgc gccgcctgaa ggtccgtctg ctaagaagcc gaagaaggaa gctagccaga aacagaaagg cggtggccag ggcaaaaaga aaaagaatca gggtaaaaag aaagcgaaga ccggcccgcc gaaccctaaa gctcagaacg gtaacaagaa aaagaccaac aagaaaccgg gcaaacgtca gcgtatggta atgaaactgg aatccgacaa gaccttccca atcatgcttg aaggcaagat taacggctac gcgtgtgtag ttggtggcaa attattccgt ccgatgcacg ttgaaggtaa gatcgataat gacgttttag ctgcgctgaa gactaagaag gcatccaaat acgatctgga atacgctgac gttcctcaga acatgagagc ggacactttc aaatatactc acgagaagcc gcagggttac tactcttggc accacggtgc tgttcagtac gagaatggtc gtttcactgt tcctaagggt gtaggcgcga aaggcgatag cggccgtccg atcctggata accagggccg tgttgtagct atagttctgg gtggcgttaa cgaaggtagc cgtaccgcac tgtccgttgt aatgtggaat gagaagggtg ttaccgttaa atacactccg gagaattgcg aacagtggnn ataaacagca cgaacaagtt ctgcagccna agcttctcga ggatncnggc tgctaacaaa gccngaaang aagctgantt ngctgcngcn nnnnnnnnnn nnnannnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMFPFQPMYP MQPMPYRNPF AAPRRPWFPR TDPFLAMQVQ ELTRSMANLT FKQRRDAPPE GPSAKKPKKE ASQKQKGGGQ GKKKKNQGKK KAKTGPPNPK AQNGNKKKTN KKPGKRQRMV MKLESDKTFP IMLEGKINGY ACVVGGKLFR PMHVEGKIDN DVLAALKTKK ASKYDLEYAD VPQNMRADTF KYTHEKPQGY YSWHHGAVQY ENGRFTVPKG VGAKGDSGRP ILDNQGRVVA IVLGGVNEGS RTALSVVMWN EKGVTVKYTP ENCEQW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttccgttc cagccgatgt acccgatgca accgatgccg tatagaaatc cgttcgctgc tccgcgtcgt ccgtggtttc ctcgtaccga cccattctta gctatgcagg ttcaggaact gactagatcc atggctaacc tgacctttaa acagcgccgt gatgcgccgc ctgaaggtcc gtctgctaag aagccgaaga aggaagctag ccagaaacag aaaggcggtg gccagggcaa aaagaaaaag aatcagggta aaaagaaagc gaagaccggc ccgccgaacc ctaaagctca gaacggtaac aagaaaaaga ccaacaagaa accgggcaaa cgtcagcgta tggtaatgaa actggaatcc gacaagacct tcccaatcat gcttgaaggc aagattaacg gctacgcgtg tgtagttggt ggcaaattat tccgtccgat gcacgttgaa ggtaagatcg ataatgacgt tttagctgcg ctgaagacta agaaggcatc caaatacgat ctggaatacg ctgacgttcc tcagaacatg agagcggaca ctttcaaata tactcacgag aagccgcagg gttactactc ttggcaccac ggtgctgttc agtacgagaa tggtcgtttc actgttccta agggtgtagg cgcgaaaggc gatagcggcc gtccgatcct ggataaccag ggccgtgttg tagctatagt tctgggtggc gttaacgaag gtagccgtac cgcactgtcc gttgtaatgt ggaatgagaa gggtgttacc gttaaataca ctccggagaa ttgcgaacag tggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for AlveA.17791.a.A22.GE33831

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMFPFQPMYP MQPMPYRNPF AAPRRPWFPR TDPFLAMQVQ ELTRSMANLT FKQRRDAPPE GPSAKKPKKE ASQKQKGGGQ GKKKKNQGKK KAKTGPPNPK AQNGNKKKTN KKPGKRQRMV MKLESDKTFP IMLEGKINGY ACVVGGKLFR PMHVEGKIDN DVLAALKTKK ASKYDLEYAD VPQNMRADTF KYTHEKPQGY YSWHHGAVQY ENGRFTVPKG VGAKGDSGRP ILDNQGRVVA IVLGGVNEXS RTALSVVMWN EKGVTVKY

Validated NT Sequence

tttnnnttnn ntttnagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtttc cgttccagcc gatgtacccg atgcaaccga tgccgtatag aaatccgttc gctgctccgc gtcgtccgtg gtttcctcgt accgacccat tcttagctat gcaggttcag gaactgacta gatccatggc taacctgacc tttaaacagc gccgtgatgc gccgcctgaa ggtccgtctg ctaagaagcc gaagaaggaa gctagccaga aacagaaagg cggtggccag ggcaaaaaga aaaagaatca gggtaaaaag aaagcgaaga ccggcccgcc gaaccctaaa gctcagaacg gtaacaagaa aaagaccaac aagaaaccgg gcaaacgtca gcgtatggta atgaaactgg aatccgacaa gaccttccca atcatgcttg aaggcaagat taacggctac gcgtgtgtag ttggtggcaa attattccgt ccgatgcacg ttgaaggtaa gatcgataat gacgttttag ctgcgctgaa gactaagaag gcatccaaat acgatctgga atacgctgac gttcctcaga acatgagagc ggacactttc aaatatactc acgagaagcc gcagggttac tactcttggc accacggtgc tgttcagtac gagaatggtc gtttcactgt tcctaagggt gtaggcgcga aaggcgatag cggccgtccg atcctggata accagggccg tgttgtagct atagttctgg gtggcgttaa cgaangtagc cgtaccgcac tgtccgttgt aatgtggaat gagaagggtg ttaccgttaa atactaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctna caaagcccga aaggaagctg anttggctgc tgcnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMFPFQPMYP MQPMPYRNPF AAPRRPWFPR TDPFLAMQVQ ELTRSMANLT FKQRRDAPPE GPSAKKPKKE ASQKQKGGGQ GKKKKNQGKK KAKTGPPNPK AQNGNKKKTN KKPGKRQRMV MKLESDKTFP IMLEGKINGY ACVVGGKLFR PMHVEGKIDN DVLAALKTKK ASKYDLEYAD VPQNMRADTF KYTHEKPQGY YSWHHGAVQY ENGRFTVPKG VGAKGDSGRP ILDNQGRVVA IVLGGVNEGS RTALSVVMWN EKGVTVKY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttccgttc cagccgatgt acccgatgca accgatgccg tatagaaatc cgttcgctgc tccgcgtcgt ccgtggtttc ctcgtaccga cccattctta gctatgcagg ttcaggaact gactagatcc atggctaacc tgacctttaa acagcgccgt gatgcgccgc ctgaaggtcc gtctgctaag aagccgaaga aggaagctag ccagaaacag aaaggcggtg gccagggcaa aaagaaaaag aatcagggta aaaagaaagc gaagaccggc ccgccgaacc ctaaagctca gaacggtaac aagaaaaaga ccaacaagaa accgggcaaa cgtcagcgta tggtaatgaa actggaatcc gacaagacct tcccaatcat gcttgaaggc aagattaacg gctacgcgtg tgtagttggt ggcaaattat tccgtccgat gcacgttgaa ggtaagatcg ataatgacgt tttagctgcg ctgaagacta agaaggcatc caaatacgat ctggaatacg ctgacgttcc tcagaacatg agagcggaca ctttcaaata tactcacgag aagccgcagg gttactactc ttggcaccac ggtgctgttc agtacgagaa tggtcgtttc actgttccta agggtgtagg cgcgaaaggc gatagcggcc gtccgatcct ggataaccag ggccgtgttg tagctatagt tctgggtggc gttaacgaag gtagccgtac cgcactgtcc gttgtaatgt ggaatgagaa gggtgttacc gttaaatact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for AlveA.17791.a.A23.GE33832

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	31kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPYRNPFAA PRRPWFPRTD PFLAMQVQEL TRSMANLTFK QRRDAPPEGP SAKKPKKEAS QKQKGGGQGK KKKNQGKKKA KTGPPNPKAQ NGNKKKTNKK PGKRQRMVMK LESDKTFPIM LEGKINGYAC VVGGKLFRPM HVEGKIDNDV LAALKTKKAS KYDLEYADVP QNMRADTFKY THEKPQGYYS WHHGAVQYEN GRFTVPKGVG AKGDSGRPIL DNQGRVVAIV LGGVNEXSRT ALSVVMWNEK GVTVKYTPEN CEQW

Validated NT Sequence

ttntnnnntt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgccgtata gaaatccgtt cgctgctccg cgtcgtccgt ggtttcctcg taccgaccca ttcttagcta tgcaggttca ggaactgact agatccatgg ctaacctgac ctttaaacag cgccgtgatg cgccgcctga aggtccgtct gctaagaagc cgaagaagga agctagccag aaacagaaag gcggtggcca gggcaaaaag aaaaagaatc agggtaaaaa gaaagcgaag accggcccgc cgaaccctaa agctcagaac ggtaacaaga aaaagaccaa caagaaaccg ggcaaacgtc agcgtatggt aatgaaactg gaatccgaca agaccttccc aatcatgctt gaaggcaaga ttaacggcta cgcgtgtgta gttggtggca aattattccg tccgatgcac gttgaaggta agatcgataa tgacgtttta gctgcgctga agactaagaa ggcatccaaa tacgatctgg aatacgctga cgttcctcag aacatgagag cggacacttt caaatatact cacgagaagc cgcagggtta ctactcttgg caccacggtg ctgttcagta cgagaatggt cgtttcactg ttcctaaggg tgtaggcgcg aaaggcgata gcggccgtcc gatcctggat aaccagggcc gtgttgtagc tatagttctg ggtggcgtta acgaangtag ccgtaccgca ctgtccgttg taatgtggaa tgagaagggt gttacngtta aatacactcc ggagaattgc gaacagtggt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccngct gctaacaaag cccgaaagga agctganttg gctgctgcna aaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPYRNPFAA PRRPWFPRTD PFLAMQVQEL TRSMANLTFK QRRDAPPEGP SAKKPKKEAS QKQKGGGQGK KKKNQGKKKA KTGPPNPKAQ NGNKKKTNKK PGKRQRMVMK LESDKTFPIM LEGKINGYAC VVGGKLFRPM HVEGKIDNDV LAALKTKKAS KYDLEYADVP QNMRADTFKY THEKPQGYYS WHHGAVQYEN GRFTVPKGVG AKGDSGRPIL DNQGRVVAIV LGGVNEGSRT ALSVVMWNEK GVTVKYTPEN CEQW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgtataga aatccgttcg ctgctccgcg tcgtccgtgg tttcctcgta ccgacccatt cttagctatg caggttcagg aactgactag atccatggct aacctgacct ttaaacagcg ccgtgatgcg ccgcctgaag gtccgtctgc taagaagccg aagaaggaag ctagccagaa acagaaaggc ggtggccagg gcaaaaagaa aaagaatcag ggtaaaaaga aagcgaagac cggcccgccg aaccctaaag ctcagaacgg taacaagaaa aagaccaaca agaaaccggg caaacgtcag cgtatggtaa tgaaactgga atccgacaag accttcccaa tcatgcttga aggcaagatt aacggctacg cgtgtgtagt tggtggcaaa ttattccgtc cgatgcacgt tgaaggtaag atcgataatg acgttttagc tgcgctgaag actaagaagg catccaaata cgatctggaa tacgctgacg ttcctcagaa catgagagcg gacactttca aatatactca cgagaagccg cagggttact actcttggca ccacggtgct gttcagtacg agaatggtcg tttcactgtt cctaagggtg taggcgcgaa aggcgatagc ggccgtccga tcctggataa ccagggccgt gttgtagcta tagttctggg tggcgttaac gaaggtagcc gtaccgcact gtccgttgta atgtggaatg agaagggtgt taccgttaaa tacactccgg agaattgcga acagtggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for AlveA.17791.a.A24.GE33833

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	30kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPYRNPFAA PRRPWFPRTD PFLAMQVQEL TRSMANLTFK QRRDAPPEGP SAKKPKKEAS QKQKGGGQGK KKKNQGKKKA KTGPPNPKAQ NGNKKKTNKK PGKRQRMVMK LESDKTFPIM LEGKINGYAC VVGGKLFRPM HVEGKIDNDV LAALKTKKAS KYDLEYADVP QNMRADTFKY THEKPQGYYS WHHGAVQYEN GRFTVPKGVG AKGDSGRPIL DNQGRVVAIV LGGVNEXSRT ALSVVMWNEK GVTVKY

Validated NT Sequence

cnnnnnnnnn nnnnnnnttt ntnnnnnttt aagaaggagn nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgccgtatag aaatccgttc gctgctccgc gtcgtccgtg gtttcctcgt accgacccat tcttagctat gcaggttcag gaactgacta gatccatggc taacctgacc tttaaacagc gccgtgatgc gccgcctgaa ggtccgtctg ctaagaagcc gaagaaggaa gctagccaga aacagaaagg cggtggccag ggcaaaaaga aaaagaatca gggtaaaaag aaagcgaaga ccggcccgcc gaaccctaaa gctcagaacg gtaacaagaa aaagaccaac aagaaaccgg gcaaacgtca gcgtatggta atgaaactgg aatccgacaa gaccttccca atcatgcttg aaggcaagat taacggctac gcgtgtgtag ttggtggcaa attattccgt ccgatgcacg ttgaaggtaa gatcgataat gacgttttag ctgcgctgaa gactaagaag gcatccaaat acgatctgga atacgctgac gttcctcaga acatgagagc ggacactttc aaatatactc acgagaagcc gcagggttac tactcttggc accacggtgc tgttcagtac gagaatggtc gtttcactgt tcctaagggt gtaggcgcga aaggcgatag cggccgtccg atcctggata accagggccg tgttgtagct atagttctgg gtggcgttaa cgaangtagc cgtaccgcac tgtccgttgt aatgtggaat gagaagggtg ttaccgttaa atactaataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg anttggctgc tgc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPYRNPFAA PRRPWFPRTD PFLAMQVQEL TRSMANLTFK QRRDAPPEGP SAKKPKKEAS QKQKGGGQGK KKKNQGKKKA KTGPPNPKAQ NGNKKKTNKK PGKRQRMVMK LESDKTFPIM LEGKINGYAC VVGGKLFRPM HVEGKIDNDV LAALKTKKAS KYDLEYADVP QNMRADTFKY THEKPQGYYS WHHGAVQYEN GRFTVPKGVG AKGDSGRPIL DNQGRVVAIV LGGVNEGSRT ALSVVMWNEK GVTVKY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgtataga aatccgttcg ctgctccgcg tcgtccgtgg tttcctcgta ccgacccatt cttagctatg caggttcagg aactgactag atccatggct aacctgacct ttaaacagcg ccgtgatgcg ccgcctgaag gtccgtctgc taagaagccg aagaaggaag ctagccagaa acagaaaggc ggtggccagg gcaaaaagaa aaagaatcag ggtaaaaaga aagcgaagac cggcccgccg aaccctaaag ctcagaacgg taacaagaaa aagaccaaca agaaaccggg caaacgtcag cgtatggtaa tgaaactgga atccgacaag accttcccaa tcatgcttga aggcaagatt aacggctacg cgtgtgtagt tggtggcaaa ttattccgtc cgatgcacgt tgaaggtaag atcgataatg acgttttagc tgcgctgaag actaagaagg catccaaata cgatctggaa tacgctgacg ttcctcagaa catgagagcg gacactttca aatatactca cgagaagccg cagggttact actcttggca ccacggtgct gttcagtacg agaatggtcg tttcactgtt cctaagggtg taggcgcgaa aggcgatagc ggccgtccga tcctggataa ccagggccgt gttgtagcta tagttctggg tggcgttaac gaaggtagcc gtaccgcact gtccgttgta atgtggaatg agaagggtgt taccgttaaa tactaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for AlveA.17791.a.A25.GE33834

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVMKLESDK TFPIMLEGKI NGYACVVGGK LFRPMHVEGK IDNDVLAALK TKKASKYDLE YADVPQNMRA DTFKYTHEKP QGYYSWHHGA VQYENGRFTV PKGVGAKGDS GRPILDNQGR VVVIVLGGVN EGSRTALSVV MWNEKGVTVK YTPENCEQW

Validated NT Sequence

attttnttta actttangaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggta atgaaactgg aatccgacaa gaccttccca atcatgcttg aaggcaagat taacggctac gcgtgtgtag ttggtggcaa attattccgt ccgatgcacg ttgaaggtaa gatcgataat gacgttttag ctgcgctgaa gactaagaag gcatccaaat acgatctgga atacgctgac gttcctcaga acatgagagc ggacactttc aaatatactc acgagaagcc gcagggttac tactcttggc accacggtgc tgttcagtac gagaatggtc gtttcactgt tcctaagggt gtaggcgcga aaggcgatag cggccgtccg atcctggata accagggccg tgttgtagtt atagttctgg gtggcgttaa cgaaggtagc cgtaccgcac tgtccgttgt aatgtggaat gagaagggtg ttaccgttaa atacactccg gagaattgcg aacagtggta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cnnnnnnnna nnnntnnacn nnnncnnnnn nnngnannnn nnnnnnncnn tnncgnncnn cnantcctan gatagnnnnn nccnnntnnn nntatnnctc nnnnngggan nnacnnannn nnncnnnnnn nntgtccnan nntaangnnn anngcnnnnn cgtnnntann nnnnnnnnnn nnnnnnnnnn ncnnnanntn nnnnncnnnn ggnnnnnnac nnngnnnnaa nnnnnnnnnn ntngcnnngc annnnnnann nnagannnnn nnatnnnggn nnngnnnnnn naaaannagn ncnnnnnann nngnnnnnnn ngnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVMKLESDK TFPIMLEGKI NGYACVVGGK LFRPMHVEGK IDNDVLAALK TKKASKYDLE YADVPQNMRA DTFKYTHEKP QGYYSWHHGA VQYENGRFTV PKGVGAKGDS GRPILDNQGR VVAIVLGGVN EGSRTALSVV MWNEKGVTVK YTPENCEQW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaatgaaa ctggaatccg acaagacctt cccaatcatg cttgaaggca agattaacgg ctacgcgtgt gtagttggtg gcaaattatt ccgtccgatg cacgttgaag gtaagatcga taatgacgtt ttagctgcgc tgaagactaa gaaggcatcc aaatacgatc tggaatacgc tgacgttcct cagaacatga gagcggacac tttcaaatat actcacgaga agccgcaggg ttactactct tggcaccacg gtgctgttca gtacgagaat ggtcgtttca ctgttcctaa gggtgtaggc gcgaaaggcg atagcggccg tccgatcctg gataaccagg gccgtgttgt agctatagtt ctgggtggcg ttaacgaagg tagccgtacc gcactgtccg ttgtaatgtg gaatgagaag ggtgttaccg ttaaatacac tccggagaat tgcgaacagt ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for AlveA.17791.a.A26.GE33835

HARVESTED ON:	10/15/2011
SEQUENCED ON:	12/5/2013
EXPECTED MW:	18kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVMKLESDK TFPIMLEGKI NGYACVVGGK LFRPMHVEGK IDNDVLAALK TKKASKYDLE YADVPQNMRA DTFKYTHEKP QGYYSWHHGA VQYENGRFTV PKGVGAKGDS GRPILDNQGR VVAIVLGGVN EGSRTALSVV MWNEKGVTV

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtaa tgaaactgga atccgacaag accttcccaa tcatgcttga aggcaagatt aacggctacg cgtgtgtagt tggtggcaaa ttattccgtc cgatgcacgt tgaaggtaag atcgataatg acgttttagc tgcgctgaag actaagaagg catccaaata cgatctggaa tacgctgacg ttcctcagaa catgagagcg gacactttca aatatactca cgagaagccg cagggttact actcttggca ccacggtgct gttcagtacg agaatggtcg tttcactgtt cctaagggtg taggcgcgaa aggcgatagc ggccgtccga tcctggataa ccagggccgt gttgtagcta tagttctggg tggcgttaac gaaggtagcc gtaccgcact gtccgttgta atgtggaatg agaagggtgt taccgtta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVMKLESDK TFPIMLEGKI NGYACVVGGK LFRPMHVEGK IDNDVLAALK TKKASKYDLE YADVPQNMRA DTFKYTHEKP QGYYSWHHGA VQYENGRFTV PKGVGAKGDS GRPILDNQGR VVAIVLGGVN EGSRTALSVV MWNEKGVTVK Y

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaatgaaa ctggaatccg acaagacctt cccaatcatg cttgaaggca agattaacgg ctacgcgtgt gtagttggtg gcaaattatt ccgtccgatg cacgttgaag gtaagatcga taatgacgtt ttagctgcgc tgaagactaa gaaggcatcc aaatacgatc tggaatacgc tgacgttcct cagaacatga gagcggacac tttcaaatat actcacgaga agccgcaggg ttactactct tggcaccacg gtgctgttca gtacgagaat ggtcgtttca ctgttcctaa gggtgtaggc gcgaaaggcg atagcggccg tccgatcctg gataaccagg gccgtgttgt agctatagtt ctgggtggcg ttaacgaagg tagccgtacc gcactgtccg ttgtaatgtg gaatgagaag ggtgttaccg ttaaatacta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for AlveA.17791.a.A41.GE33836

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPYMARCIR CAVGSCHSPI AIEAVKSDGH DGYVRLQTSS QYGLDSSGNL KGRTMRYDMH GTIKEIPLHQ VSLHTSRPCH IVDGHGYFLL ARCPAGDSIT MEFKKDSVTH SCSVPYEVKF NPVGRELYTH PPEHGVEQAC QVYAHDAQNR GAYVEMHLPG SEVDSSLVSL SGSSVTVTPP VGTSALVECE CGGTKISETI NKTKQFSQCT KKEQCRAYRL QNDKWVYNSD KLPKAAGATL KGKLHVPFLL ADGKCTVPLA PEPMITFGFR SVSLKLHPKN PTYLTTRQLA DEPHYTHELI SEPAVRNFTV TEKGWEFVWG NHPPKRFWAQ ETAPGNPHGL PHEVITHYYH RYPMSTILGL SICAAIATVS VAASTWLFCR SRVACLTPYR LTPNARIPFC LAVLCC

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgtacatg gctagatgca ttcgctgcgc tgtaggctcc tgccactctc ctatagctat cgaagcagtt aaatccgacg gtcacgacgg ctacgttcgt ctgcagacta gctctcaata cggtctggac tcctccggca acctgaaagg ccgtaccatg agatacgaca tgcacggtac catcaaagaa atcccgctgc accaggttag cctgcacaca tctcgtccgt gtcacatagt tgacggtcac ggttatttcc ttctggctcg ctgcccggct ggcgactcca tcaccatgga atttaagaaa gatagcgtaa cccactcttg ctctgttccg tacgaagtta agttcaaccc ggtaggccgt gaactgtaca cccacccgcc tgaacacggt gttgaacagg catgccaggt atacgctcac gacgctcaga accgcggcgc ttacgttgaa atgcatctgc cgggtagcga agttgactct tctttagtta gcctgtccgg cagctccgtt accgtcactc ctcctgttgg tacctccgct ttagttgaat gcgaatgcgg cggcaccaag attagcgaga ccattaacaa gactaaacag ttctctcagt gcaccaagaa agaacagtgc cgtgcttacc gtctccagaa cgacaagtgg gtttataact ccgacaaact gccgaaggcg gctggtgcta ccctgaaagg taaactgcac gtcccgttcc tgttagctga cggtaaatgc actgtaccgc tggcgcctga accgatgata accttcggtt ttagatctgt ttccctgaaa ttgcatccga agaatccgac ctacctgacc actcgtcagt tagctgacga accgcactac acccacgaac tgattagcga accagcggta cgtaacttca ctgtaaccga gaagggttgg gaattcgtgt ggggtaacca tccgcctaaa cgcttctggg cgcaagaaac tgcaccgggt aacccgcacg gcctgccgca tgaagtaatc acccactact accaccgtta cccgatgtcc accatcctgg gtctctctat ctgtgctgct atagctaccg ttagcgtagc cgcaagcact tggctgttct gccgttcccg tgttgcttgc ctgactcctt atagactgac tccgaacgct cgtatcccgt tctgcctggc tgttctgtgc tgttaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for AlveA.17791.a.A42.GE33837

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPYMARCIR CAVGSCHSPI AIEAVKSDGH DGYVRLQTSS QYGLDSSGNL KGRTMRYDMH GTIKEIPLHQ VSLHTSRPCH IVDGHGYFLL ARCPAGDSIT MEFKKDSVTH SCSVPYEVKF NPVGRELYTH PPEHGVEQAC QVYAHDAQNR GAYVEMHLPG SEVDSSLVSL SGSSVTVTPP VGTSALVECE CGGTKISETI NKTKQFSQCT KKEQCRAYRL QNDKWVYNSD KLPKAAGATL KGKLHVPFLL ADGKCTVPLA PEPMITFGFR SVSLKLHPKN PTYLTTRQLA DEPHYTHELI SEPAVRNFTV TEKGWEFVWG NHPPKRFWAQ ETAPGNPHGL PHEVITHYYH RYPMSTILGL SICAAIATVS VAASTWLFCR SRVACLT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgtacatg gctagatgca ttcgctgcgc tgtaggctcc tgccactctc ctatagctat cgaagcagtt aaatccgacg gtcacgacgg ctacgttcgt ctgcagacta gctctcaata cggtctggac tcctccggca acctgaaagg ccgtaccatg agatacgaca tgcacggtac catcaaagaa atcccgctgc accaggttag cctgcacaca tctcgtccgt gtcacatagt tgacggtcac ggttatttcc ttctggctcg ctgcccggct ggcgactcca tcaccatgga atttaagaaa gatagcgtaa cccactcttg ctctgttccg tacgaagtta agttcaaccc ggtaggccgt gaactgtaca cccacccgcc tgaacacggt gttgaacagg catgccaggt atacgctcac gacgctcaga accgcggcgc ttacgttgaa atgcatctgc cgggtagcga agttgactct tctttagtta gcctgtccgg cagctccgtt accgtcactc ctcctgttgg tacctccgct ttagttgaat gcgaatgcgg cggcaccaag attagcgaga ccattaacaa gactaaacag ttctctcagt gcaccaagaa agaacagtgc cgtgcttacc gtctccagaa cgacaagtgg gtttataact ccgacaaact gccgaaggcg gctggtgcta ccctgaaagg taaactgcac gtcccgttcc tgttagctga cggtaaatgc actgtaccgc tggcgcctga accgatgata accttcggtt ttagatctgt ttccctgaaa ttgcatccga agaatccgac ctacctgacc actcgtcagt tagctgacga accgcactac acccacgaac tgattagcga accagcggta cgtaacttca ctgtaaccga gaagggttgg gaattcgtgt ggggtaacca tccgcctaaa cgcttctggg cgcaagaaac tgcaccgggt aacccgcacg gcctgccgca tgaagtaatc acccactact accaccgtta cccgatgtcc accatcctgg gtctctctat ctgtgctgct atagctaccg ttagcgtagc cgcaagcact tggctgttct gccgttcccg tgttgcttgc ctgacttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for AlveA.17791.a.A43.GE33838

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPYMARCIR CAVGSCHSPI AIEAVKSDGH DGYVRLQTSS QYGLDSSGNL KGRTMRYDMH GTIKEIPLHQ VSLHTSRPCH IVDGHGYFLL ARCPAGDSIT MEFKKDSVTH SCSVPYEVKF NPVGRELYTH PPEHGVEQAC QVYAHDAQNR GAYVEMHLPG SEVDSSLVSL SGSSVTVTPP VGTSALVECE CGGTKISETI NKTKQFSQCT KKEQCRAYRL QNDKWVYNSD KLPKAAGATL KGKLHVPFLL ADGKCTVPLA PEPMITFGFR SVSLKLHPKN PTYLXTRQLA DEPHYTHEXI SEXAXRXXXV XXXXGXXXGX XXXXLLGXXX AXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX

Validated NT Sequence

ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgccgta catggctaga tgcattcgct gcgctgtagg ctcctgccac tctcctatag ctatcgaagc agttaaatcc gacggtcacg acggctacgt tcgtctgcag actagctctc aatacggtct ggactcctcc ggcaacctga aaggccgtac catgagatac gacatgcacg gtaccatcaa agaaatcccg ctgcaccagg ttagcctgca cacatctcgt ccgtgtcaca tagttgacgg tcacggttat ttccttctgg ctcgctgccc ggctggcgac tccatcacca tggaatttaa gaaagatagc gtaacccact cttgctctgt tccgtacgaa gttaagttca acccggtagg ccgtgaactg tacacccacc cgcctgaaca cggtgttgaa caggcatgcc aggtatacgc tcacgacgct cagaaccgcg gcgcttacgt tgaaatgcat ctgccgggta gcgaagttga ctcttcttta gttagcctgt ccggcagctc cgttaccgtc actcctcctg ttggtacctc cgctttagtt gaatgcgaat gcggcggcac caagattagc gagaccatta acaagactaa acagttctct cagtgcacca agaaagaaca gtgccgtgct taccgtctcc agaacgacaa gtgggtttat aactccgaca aactgccgaa ggcggctggt gctaccctga aaggtaaact gcacgtcccg ttcctgttag ctgacggtaa atgcactgta ccgctggcgc ctgaaccgat gataaccttc ggttttagat ctgtttccct gaaattgcat ccgaagaatc cgacntacct gannactcgt cagttagctg acgaaccgca ctacacccac gaantgatta gcgaannagc ggnacgtnnc tncnctgtan ngannnggtn gggnnnnnnn gnngggnnnn tncncnaanc gcttctgggc ncanaanctg cnnnnnnncn nnnncnnnnn nncannanna nnnncnacna nnnnnnncnc nannnnnnnc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn ncnnnccnan nnnnnnnnnn nnnnnnnnnn gnnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPYMARCIR CAVGSCHSPI AIEAVKSDGH DGYVRLQTSS QYGLDSSGNL KGRTMRYDMH GTIKEIPLHQ VSLHTSRPCH IVDGHGYFLL ARCPAGDSIT MEFKKDSVTH SCSVPYEVKF NPVGRELYTH PPEHGVEQAC QVYAHDAQNR GAYVEMHLPG SEVDSSLVSL SGSSVTVTPP VGTSALVECE CGGTKISETI NKTKQFSQCT KKEQCRAYRL QNDKWVYNSD KLPKAAGATL KGKLHVPFLL ADGKCTVPLA PEPMITFGFR SVSLKLHPKN PTYLTTRQLA DEPHYTHELI SEPAVRNFTV TEKGWEFVWG NHPPKRFWAQ ETAPGNPHGL PHEVITHYYH RYPMSTILGL SICAAIATVS VAASTWLFCR SRVACLTPYR LTPNARIP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgtacatg gctagatgca ttcgctgcgc tgtaggctcc tgccactctc ctatagctat cgaagcagtt aaatccgacg gtcacgacgg ctacgttcgt ctgcagacta gctctcaata cggtctggac tcctccggca acctgaaagg ccgtaccatg agatacgaca tgcacggtac catcaaagaa atcccgctgc accaggttag cctgcacaca tctcgtccgt gtcacatagt tgacggtcac ggttatttcc ttctggctcg ctgcccggct ggcgactcca tcaccatgga atttaagaaa gatagcgtaa cccactcttg ctctgttccg tacgaagtta agttcaaccc ggtaggccgt gaactgtaca cccacccgcc tgaacacggt gttgaacagg catgccaggt atacgctcac gacgctcaga accgcggcgc ttacgttgaa atgcatctgc cgggtagcga agttgactct tctttagtta gcctgtccgg cagctccgtt accgtcactc ctcctgttgg tacctccgct ttagttgaat gcgaatgcgg cggcaccaag attagcgaga ccattaacaa gactaaacag ttctctcagt gcaccaagaa agaacagtgc cgtgcttacc gtctccagaa cgacaagtgg gtttataact ccgacaaact gccgaaggcg gctggtgcta ccctgaaagg taaactgcac gtcccgttcc tgttagctga cggtaaatgc actgtaccgc tggcgcctga accgatgata accttcggtt ttagatctgt ttccctgaaa ttgcatccga agaatccgac ctacctgacc actcgtcagt tagctgacga accgcactac acccacgaac tgattagcga accagcggta cgtaacttca ctgtaaccga gaagggttgg gaattcgtgt ggggtaacca tccgcctaaa cgcttctggg cgcaagaaac tgcaccgggt aacccgcacg gcctgccgca tgaagtaatc acccactact accaccgtta cccgatgtcc accatcctgg gtctctctat ctgtgctgct atagctaccg ttagcgtagc cgcaagcact tggctgttct gccgttcccg tgttgcttgc ctgactcctt atagactgac tccgaacgct cgtatcccgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for AlveA.17791.a.A51.GE33842

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHATTMPSQ AGISYNTIVN RAGYAPLPIS ITPTKIKLIP TVNLEYVTCH YKTGMDSPAI KCCGSQECTP TYRPDEQCKV FTGVYPFMWG GAYCFCDTEN TQVSKAYVMK SDDCLADHAE AYKAHTASVQ AFLNITVGEH SIVTTVYVNG ETPVNFNGVK LTAGPLSTAW TPFDRKIVQY AGEIYNYDFP EYGAGQPGAF GDIQSRTVSS SDLYANTNLV LQRPKAGAIH VPYTQAPSGF EQWKKDKAPS LKFTAPFGCE IYTNPIRAEN CAVGSIPLAF DIPDALFTRV SETPTLSAAE CTLNECVYSS DFGGIATVKY SASKSGKCAV HVPSGTATLK EAAVELTEQG SATIHFSTAN IHPEFRLQIC TSYVTCKGDC HPPKDHIVTH PQYHAQTFTA AVSKTAWTWL TSLL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacgcaacc accatgccta gccaggctgg catctcctat aacactatag ttaaccgcgc gggttacgct ccgctgccaa tctccatcac tcctaccaag attaaactga tccctaccgt taacctggaa tacgtgactt gtcactacaa gactggcatg gactctccgg ctattaagtg ctgtggctcg caggaatgca ctccgactta tagacctgac gaacagtgca aagtgttcac cggcgtctac ccgtttatgt ggggcggtgc ttactgcttc tgcgacactg agaatacgca ggttagcaaa gcttacgtaa tgaaatctga cgattgcctg gctgatcacg ctgaagctta caaagcccat actgcatccg ttcaagcgtt ccttaacatc actgttggtg aacatagcat agttaccact gtttacgtaa atggtgaaac tccggttaac ttcaacggtg ttaaactgac tgcgggtccg ctgtctaccg cttggactcc gtttgataga aagattgttc agtacgctgg cgaaatctac aactacgact ttccggaata cggcgctggc cagccaggtg ctttcggcga tatccagtcc cgtaccgtgt cctcttccga cctgtacgct aacactaact tagttctgca gcgtccgaaa gccggcgcga tccacgtccc gtacactcag gcaccgagcg gttttgaaca gtggaagaag gataaagcac catccctgaa attcaccgct ccgttcggtt gcgaaatcta taccaacccg attcgtgcgg agaattgtgc tgtaggctct atcccgctgg cattcgacat cccggacgcg ctgttcaccc gtgtttccga aactcctacc ctgagcgcag ctgaatgcac cctgaacgag tgcgtctata gctctgattt cggtggtata gctaccgtta agtactctgc gtccaagtct ggtaaatgcg ctgttcacgt tcctagcggt accgctaccc tgaaagaagc tgcagttgaa ctgaccgagc agggttctgc tactattcac ttctccacag caaacatcca cccggaattt agactgcaga tctgcacctc ctatgtaacc tgcaaaggcg actgtcaccc tccgaaagac cacatagtta cccacccaca gtaccacgct cagactttca ccgctgctgt tagcaagact gcttggactt ggctgacctc ccttctgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for AlveA.17791.a.A52.GE33843

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	38kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHATTMPSQ AGISYNTIVN RAGYAPLPIS ITPTKIKLIP TVNLEYVTCH YKTGMDSPAI KCCGSQECTP TYRPDEQCKV FTGVYPFMWG GAYCFCDTEN TQVSKAYVMK SDDCLADHAE AYKAHTASVQ AFLNITVGEH SIVTTVYVNG ETPVNFNGVK LTAGPLSTAW TPFDRKIVQY AGEIYNYDFP EYGAGQPGAF GDIQSRTVSS SDLYANTNLV LQRPKAGAIH VPYTQAPSGF EQWKKDKAPS LKFTAPFGCE IYTNPIRAEN CAVGSIPLAF DIPDALFTRV SETPTLSAAE CTLNECVYSS DFGGIATVKY SASKSGKCAV HVPSGTATL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacgcaacc accatgccta gccaggctgg catctcctat aacactatag ttaaccgcgc gggttacgct ccgctgccaa tctccatcac tcctaccaag attaaactga tccctaccgt taacctggaa tacgtgactt gtcactacaa gactggcatg gactctccgg ctattaagtg ctgtggctcg caggaatgca ctccgactta tagacctgac gaacagtgca aagtgttcac cggcgtctac ccgtttatgt ggggcggtgc ttactgcttc tgcgacactg agaatacgca ggttagcaaa gcttacgtaa tgaaatctga cgattgcctg gctgatcacg ctgaagctta caaagcccat actgcatccg ttcaagcgtt ccttaacatc actgttggtg aacatagcat agttaccact gtttacgtaa atggtgaaac tccggttaac ttcaacggtg ttaaactgac tgcgggtccg ctgtctaccg cttggactcc gtttgataga aagattgttc agtacgctgg cgaaatctac aactacgact ttccggaata cggcgctggc cagccaggtg ctttcggcga tatccagtcc cgtaccgtgt cctcttccga cctgtacgct aacactaact tagttctgca gcgtccgaaa gccggcgcga tccacgtccc gtacactcag gcaccgagcg gttttgaaca gtggaagaag gataaagcac catccctgaa attcaccgct ccgttcggtt gcgaaatcta taccaacccg attcgtgcgg agaattgtgc tgtaggctct atcccgctgg cattcgacat cccggacgcg ctgttcaccc gtgtttccga aactcctacc ctgagcgcag ctgaatgcac cctgaacgag tgcgtctata gctctgattt cggtggtata gctaccgtta agtactctgc gtccaagtct ggtaaatgcg ctgttcacgt tcctagcggt accgctaccc tgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for AlveA.17791.a.A53.GE33844

HARVESTED ON:	10/15/2011
SEQUENCED ON:	10/21/2011
EXPECTED MW:	45kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	84
PERCENT COVERAGE:	92

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHATTMPSQ AGISYNTIVN RAGYAPLPIS ITPTKIKLIP TVNLEYVTCH YKTGMDSPAI KCCGSQECTP TYRPDEQCKV FTGVYPFMWG GAYCFCDTEN TQVSKAYVMK SDDCLADHAE AYKAHTASVQ AFLNITVGEH SIVTTVYVNG ETPVNFNGVK LTAGPLSTAW TPFDRKIVQY AGEIYNYDFP EYGAGQPGAF GDIQSRTVSS SDLYANTNLV LQRPKAGAIH VPYTQAPSGF EQWKKDKAPS LKFTAPFGCE IYTNPIRAXN CAVXSIPLAF DIPDALXTRV SETPTLSAAE XTLNXXVYXS XXVXXLXXTL RXXGXALXXX XXXXXXXXXX XXLXXXXXXX XXXXXXXXXX XXXXX

Validated NT Sequence

attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcacg caaccaccat gcctagccag gctggcatct cctataacac tatagttaac cgcgcgggtt acgctccgct gccaatctcc atcactccta ccaagattaa actgatccct accgttaacc tggaatacgt gacttgtcac tacaagactg gcatggactc tccggctatt aagtgctgtg gctcgcagga atgcactccg acttatagac ctgacgaaca gtgcaaagtg ttcaccggcg tctacccgtt tatgtggggc ggtgcttact gcttctgcga cactgagaat acgcaggtta gcaaagctta cgtaatgaaa tctgacgatt gcctggctga tcacgctgaa gcttacaaag cccatactgc atccgttcaa gcgttcctta acatcactgt tggtgaacat agcatagtta ccactgttta cgtaaatggt gaaactccgg ttaacttcaa cggtgttaaa ctgactgcgg gtccgctgtc taccgcttgg actccgtttg atagaaagat tgttcagtac gctggcgaaa tctacaacta cgactttccg gaatacggcg ctggccagcc aggtgctttc ggcgatatcc agtcccgtac cgtgtcctct tccgacctgt acgctaacac taacttagtt ctgcagcgtc cgaaagccgg cgcgatccac gtcccgtaca ctcaggcacc gagcggtttt gaacagtgga agaaggataa agcaccatcc ctgaaattca ccgctccgtt cggttgcgaa atctatacca acccgattcg tgcgganaat tgtgctgtan gctctatccc gctggcattc gacatcccgg acgcgctgnt cacccgtgtt tccgaaactc ctaccctgag cgcagctgaa ngcaccctga acganngcgt ctatngctct nattnggtgg nnnngctncn nnnnactctg cgtcnnnntg gnaangcnct tannngnncn ntnnnnnnan nancnncann nanngnncnn nnnnggctan nnnnnnnnnn nnnnnannnn nnnnnnnnnn nnnnnnnnnn nnnccnnnnn nnnnnnnnnn nnnnnng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHATTMPSQ AGISYNTIVN RAGYAPLPIS ITPTKIKLIP TVNLEYVTCH YKTGMDSPAI KCCGSQECTP TYRPDEQCKV FTGVYPFMWG GAYCFCDTEN TQVSKAYVMK SDDCLADHAE AYKAHTASVQ AFLNITVGEH SIVTTVYVNG ETPVNFNGVK LTAGPLSTAW TPFDRKIVQY AGEIYNYDFP EYGAGQPGAF GDIQSRTVSS SDLYANTNLV LQRPKAGAIH VPYTQAPSGF EQWKKDKAPS LKFTAPFGCE IYTNPIRAEN CAVGSIPLAF DIPDALFTRV SETPTLSAAE CTLNECVYSS DFGGIATVKY SASKSGKCAV HVPSGTATLK EAAVELTEQG SATIHFSTAN IHPEFRLQIC TSYVTCKGDC HPPKDHIVTH PQYHAQT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacgcaacc accatgccta gccaggctgg catctcctat aacactatag ttaaccgcgc gggttacgct ccgctgccaa tctccatcac tcctaccaag attaaactga tccctaccgt taacctggaa tacgtgactt gtcactacaa gactggcatg gactctccgg ctattaagtg ctgtggctcg caggaatgca ctccgactta tagacctgac gaacagtgca aagtgttcac cggcgtctac ccgtttatgt ggggcggtgc ttactgcttc tgcgacactg agaatacgca ggttagcaaa gcttacgtaa tgaaatctga cgattgcctg gctgatcacg ctgaagctta caaagcccat actgcatccg ttcaagcgtt ccttaacatc actgttggtg aacatagcat agttaccact gtttacgtaa atggtgaaac tccggttaac ttcaacggtg ttaaactgac tgcgggtccg ctgtctaccg cttggactcc gtttgataga aagattgttc agtacgctgg cgaaatctac aactacgact ttccggaata cggcgctggc cagccaggtg ctttcggcga tatccagtcc cgtaccgtgt cctcttccga cctgtacgct aacactaact tagttctgca gcgtccgaaa gccggcgcga tccacgtccc gtacactcag gcaccgagcg gttttgaaca gtggaagaag gataaagcac catccctgaa attcaccgct ccgttcggtt gcgaaatcta taccaacccg attcgtgcgg agaattgtgc tgtaggctct atcccgctgg cattcgacat cccggacgcg ctgttcaccc gtgtttccga aactcctacc ctgagcgcag ctgaatgcac cctgaacgag tgcgtctata gctctgattt cggtggtata gctaccgtta agtactctgc gtccaagtct ggtaaatgcg ctgttcacgt tcctagcggt accgctaccc tgaaagaagc tgcagttgaa ctgaccgagc agggttctgc tactattcac ttctccacag caaacatcca cccggaattt agactgcaga tctgcacctc ctatgtaacc tgcaaaggcg actgtcaccc tccgaaagac cacatagtta cccacccaca gtaccacgct cagacttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

CENTER ID:	AlveA.17791.a
ORGANISM:	Alphavirus Venezuelan equine encephalitis virus Trinidad donkey
ASSOCIATED DISEASE:
CURRENT STATUS:	HSQC satisfactory
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/IIIC

AlveA.17791.a

structural polyprotein precursor

Ordering Clones & Proteins

Clones*

Proteins

AA Sequence

NT Sequence

PURIFICATION DATe:	12/2/2011
CONCENTRATION:	61.98mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	50µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98