AclwA.19027.a

Uncharacterized protein

CENTER ID:	AclwA.19027.a
ORGANISM:	Acinetobacter lwoffii NIPH 478
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
AclwA.19027.a.B1.PW38377	full length		1	336

Molecular models

METHOD	RESULTS
comparative modelling	Robetta_80932

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1217668.3.peg.251
UniProt:	N9HQY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRDTISIHF VNAALSGVKR LGMDVDTLLS HVGIEAELLH QPKARISPEQ YTRFIKMLWM VTQDEHVGFD KQQRRLGTFA IMCQLIIHAK TLGDALELSS QFYKLFGDEW SVTLERDKHE ARLVPLIPNA MDPDHFITES MLMIWHGLAS WLIERRLPLE RVHFSYPRPA HADEYDALFF APVMQFDMPR TEITFAADYL DLPIRQNEET LEEFLKAAPA QLLVKFKNTN SLTSRIRDVL KSQIGEEMPT LNDVASMLYL SPQTLRRRLA AEGKSYQGVK DALRRDAAIH LLLNPDLTLE DVAQQVGFSE TSTFHRAFKK WTGVTPGLYR QLHGYH

NT Sequence

atgagtcgtg atacgatcag tatccacttc gtcaatgcag ccttatctgg cgttaagcgt ctgggcatgg atgtcgatac tttattgtct cacgtcggga ttgaagcaga attgctgcat cagcccaaag cgcgtatttc gcctgaacaa tatacccgct ttattaaaat gctgtggatg gtgacccagg acgagcatgt cggctttgac aaacagcaac gccggttggg tacttttgcg atcatgtgcc agctcatcat tcatgccaaa accctcggtg atgcactaga actttcttcc cagttctaca aactctttgg tgatgaatgg tcggtgaccc tggagcgtga caaacacgaa gctcgtcttg ttccactcat tccaaatgca atggatccgg atcattttat tactgaaagc atgttgatga tctggcatgg cttggcgtct tggctcattg aacgtcgcct gccactggaa cgcgtgcatt ttagctatcc acgtccagcc catgccgatg aatatgatgc cctgttcttt gcgccggtga tgcagttcga catgccacgt accgaaatta cttttgctgc ggactatctg gacctgccaa ttcgtcagaa tgaagaaacc ttagaagagt ttttgaaagc tgcgccggca caattgctag tgaaattcaa aaacaccaac tcgctgactt cacggattcg tgatgtgctg aagagccaga ttggggaaga aatgccgaca ttaaacgatg tcgcgtcgat gttatatcta tcaccacaaa ccttgcgccg ccgtctggca gccgaaggca aaagctatca aggcgtaaaa gatgccttac gccgtgatgc tgcaattcac ttattactta atccggatct aacccttgaa gatgttgcac aacaagtcgg tttcagtgaa actagtactt tccaccgcgc cttcaagaaa tggaccggtg taaccccggg tctgtatcgt caattgcatg gctatcatta a

Details for AclwA.19027.a.B1.PW38377

PURIFICATION DATe:	10/23/2017
CONCENTRATION:	21.79mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	16
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

SEC UV problems, no 260nM result

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMS RDTISIHFVN AALSGVKRLG MDVDTLLSHV GIEAELLHQP KARISPEQYT RFIKMLWMVT QDEHVGFDKQ QRRLGTFAIM CQLIIHAKTL GDALELSSQF YKLFGDEWSV TLERDKHEAR LVPLIPNAMD PDHFITESML MIWHGLASWL IERRLPLERV HFSYPRPAHA DEYDALFFAP VMQFDMPRTE ITFAADYLDL PIRQNEETLE EFLKAAPAQL LVKFKNTNSL TSRIRDVLKS QIGEEMPTLN DVASMLYLSP QTLRRRLAAE GKSYQGVKDA LRRDAAIHLL LNPDLTLEDV AQQVGFSETS TFHRAFKKWT GVTPGLYRQL HGYH

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTC GTGATACGAT CAGTATCCAC TTCGTCAATG CAGCCTTATC TGGCGTTAAG CGTCTGGGCA TGGATGTCGA TACTTTATTG TCTCACGTCG GGATTGAAGC AGAATTGCTG CATCAGCCCA AAGCGCGTAT TTCGCCTGAA CAATATACCC GCTTTATTAA AATGCTGTGG ATGGTGACCC AGGACGAGCA TGTCGGCTTT GACAAACAGC AACGCCGGTT GGGTACTTTT GCGATCATGT GCCAGCTCAT CATTCATGCC AAAACCCTCG GTGATGCACT AGAACTTTCT TCCCAGTTCT ACAAACTCTT TGGTGATGAA TGGTCGGTGA CCCTGGAGCG TGACAAACAC GAAGCTCGTC TTGTTCCACT CATTCCAAAT GCAATGGATC CGGATCATTT TATTACTGAA AGCATGTTGA TGATCTGGCA TGGCTTGGCG TCTTGGCTCA TTGAACGTCG CCTGCCACTG GAACGCGTGC ATTTTAGCTA TCCACGTCCA GCCCATGCCG ATGAATATGA TGCCCTGTTC TTTGCGCCGG TGATGCAGTT CGACATGCCA CGTACCGAAA TTACTTTTGC TGCGGACTAT CTGGACCTGC CAATTCGTCA GAATGAAGAA ACCTTAGAAG AGTTTTTGAA AGCTGCGCCG GCACAATTGC TAGTGAAATT CAAAAACACC AACTCGCTGA CTTCACGGAT TCGTGATGTG CTGAAGAGCC AGATTGGGGA AGAAATGCCG ACATTAAACG ATGTCGCGTC GATGTTATAT CTATCACCAC AAACCTTGCG CCGCCGTCTG GCAGCCGAAG GCAAAAGCTA TCAAGGCGTA AAAGATGCCT TACGCCGTGA TGCTGCAATT CACTTATTAC TTAATCCGGA TCTAACCCTT GAAGATGTTG CACAACAAGT CGGTTTCAGT GAAACTAGTA CTTTCCACCG CGCCTTCAAG AAATGGACCG GTGTAACCCC GGGTCTGTAT CGTCAATTGC ATGGCTATCA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT