AccaA.20413.a

phosphoinositide 3kinase family, accessory domain (pik domain) domain containing protein (PI3K)

CENTER ID: AccaA.20413.a
ORGANISM: Acanthamoeba castellanii
ASSOCIATED DISEASE: Amoebic keratitis and encephalomyelitis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
AccaA.20413.a.B2.GE43865 kinase domain, longest version 568 1376
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:ACA1_380980
UniProt: L8GQK9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTDTVKFSK LFENAVLKVQ VVIAGETHAY EFPATSTVDH VKYEIFKRTP TLKGKELNDY FLALNEDDVL HVEFVKLVWS HEQIHNAFLG ETPVQLQLQP RDKFELYGGK KGSSVRPAGS RRDPPKPADN LSTTKPTTTA PDTVDVILSD FTSRLDKLFD DAKSTLTAAE EAAIGEKLRR LNGNKNDAIA HLEKVLEALR KGAGEARLLH KAELSSLPAE DDVKPTNGHS DSADAEAEQV IEVQHASGDR AAAVDGGELE QAKARPALMT SSSSFPSLPS IFDDRPLAGT PQPFRKYGAR DRAGSPTGNQ LPQQRQPATG SGGSIPLTSQ RSRKESSPTA RRRPALALGS SGTIPPEETN GRLKSTYFNP QVRKIALGSQ RTSRMEKLRN IQIGSLIGKP LGWTSGEEEV RDFRAKMARL FDFDNWELGE KGFANIQQPF RKYGARDRAG SPTGNQLPQQ RQPATGSGGS IPLTSQRSRK ESSPTARRRP ALALGSSGTI PPEETNGRLK STYFNPQVRK IALGSQRTSR MPLGWTSGEE EVRDFRAKMA RLFDFDNWEL GEKGFANIQQ VRVKLHFPSG SLVRTLLCSA DDTVYDLVAT GLSSVKIRQA MSISGEVGDY LVKICGYSWY ILNLDTKLRS VDYVRESIKR NKEVEFVLVE RSSFKRTDLQ ELVYLDHFQD VDLGMDTEPF DDDEDPSLAG GFGEKKHIPL SEIKRNFELR VICLEDADFG EVPAMQQLPK GVPVHFYVSA ALYHGGESLA PEMKTTATPS MLWCQWLRLN LQMCQIPKAT RLCLSLMGVN PNDGSEFPLG WVNLNLVDYN GNLRMGVTSL NLWPGEKANP IGTCVPNYKS KNPIKLIVEL YKFPHVVKYK RPILVAPAYE DNPYPRAPER KRLVHIISQD SLHMPSSEEK KLLWEYRRTL APNPKALAKV LMAINWSRPE DVAEAHRMLT YWTPLSPIEA LELLDAYFAD EVVREYAVAK LCQLSLDELQ AYLPQLTQVL KYEPYHDSAL ARFLLAAAFR HRPTIGHFFF WHLQAELQVY LRGCGQQREG LLKQMEVSSK LGRIANLMKP LPFKERKQAL QNELVAAGLP SHFILPMNPA LEARDLNCDE CKTLESFTAP LFLSFDNNDP AGSPFKVIFK AGDDLRQDIL ILQMFGIMDR LWKKHGLDLH MTLYGCTATG EAAGFIEVKA GGASAVFKDS VLTNWLKKYN PQEDQWAGVE DNFIRSCAGY CVATYVLGIG DRHNDNIMVT YSGHLFHIDF AHFLGNMMEW KGFKRETAPF VFTPQFLHVM GGEKGAGFQR FVSLSCLAYN IIRRYAHMLS TGIKELKGVE DLHYLREVLA LNMSDEKAAE YFTTKIYESL KTKRTQFNDA IHILANNKKN E
NT Sequence
atgtctaccg atacagtgaa gttttccaag ttgttcgaga acgcggtgct caaggtccag gtggtcatcg ctggtgagac gcacgcctac gagtttccgg ccacctcgac cgtcgatcat gtcaaatacg agatcttcaa gaggacgccc acgctcaaag gcaaggagct caacgactac tttctcgcct tgaacgagga tgacgtgttg cacgtcgagt tcgtcaaact cgtttggtcg cacgaacaaa tacacaacgc cttcctcggc gagacgcccg tgcagctaca gctgcagccg cgcgacaagt tcgagttgta tggcggcaag aaggggagca gcgtgcgacc ggcgggcagt cgaagagacc cgccgaagcc cgccgacaac ctgtcgacaa caaaacccac cacgaccgca cccgacacag tcgacgtcat cctgagcgac ttcacatcac ggttggacaa actgttcgat gacgccaaga gcacgctgac ggccgccgag gaggcagcga tcggcgaaaa gctgcgaagg ctcaacggca acaagaacga cgccatcgcg cacctcgaaa aggtgctgga ggccctgcgc aagggcgcgg gcgaggcgcg actgcttcac aaggctgagc tctcctcgct gccggccgaa gacgacgtca aacccacgaa cggtcacagt gacagtgccg acgctgaggc cgagcaggtc atcgaggtgc agcacgcaag cggcgatcga gctgcagctg tggatggtgg tgagttggag caggccaagg caaggcccgc gttgatgacg tcgtcgtctt ccttcccgtc gctcccctcc attttcgacg accgacccct ggccggcacg ccgcagccgt tccgcaagta cggcgcgcgc gaccgagcgg gctcgccgac cggcaaccag ctcccgcagc agcgacagcc cgccaccggc agcggcggat cgattcccct cacatcacag cgctcgcgca aggagtcgtc gcccaccgcc cggcgccggc cagcgctggc gctgggaagc tcgggcacga taccgcctga ggagaccaat ggccgactga agagcaccta cttcaaccca caggtccgca agatcgcgct tgggtcccag cgaacctcga gaatggaaaa actgaggaac attcaaatcg ggtctctgat cggcaagccc ctcgggtgga ccagcggcga ggaggaagtg cgcgacttcc gtgcgaagat ggcgcgactg ttcgactttg acaactggga gctgggcgaa aagggattcg ccaacatcca gcagccgttc cgcaagtacg gcgcgcgcga ccgagcgggc tcgccgaccg gcaaccagct cccgcagcag cgacagcccg ccaccggcag cggcggatcg attcccctca catcacagcg ctcgcgcaag gagtcgtcgc ccaccgcccg gcgccggcca gcgctggcgc tgggaagctc gggcacgata ccgcctgagg agaccaatgg ccgactgaag agcacctact tcaatccaca ggtccgcaag atcgcgcttg ggtcccagcg aacctcgaga atgcccctcg ggtggaccag cggcgaggag gaagtgcgcg acttccgtgc gaagatggcg cgactgttcg actttgacaa ctgggagctg ggcgaaaagg gattcgccaa catccagcag gtgcgggtga agctccactt cccctcgggc tcgctcgtgc gcaccctgct ctgctccgcc gacgacaccg tctatgattt ggtggcgact ggtctgtcga gcgtcaagat ccgacaggcg atgtccatct cgggcgaagt cggcgactac ctggtcaaga tctgcggata ctcgtggtac attctcaacc tcgacaccaa gctgcggagc gtggactacg tgcgcgagag catcaagcgc aacaaggagg tcgagttcgt cctggtcgag cggtcctcct tcaaacgaac cgatctccag gaactggtct acctcgacca cttccaggac gtcgatttgg ggatggacac cgagccgttc gatgacgacg aagatccgtc cctcgccggc ggattcggtg agaagaaaca catcccgctc tctgaaatca agcgcaactt tgagcttagg gtcatttgcc tggaggatgc ggactttggc gaggtgccag cgatgcagca gctccccaag ggcgtgcccg tccacttcta cgtctctgct gctctctacc acggcggcga gagtttggcg ccggagatga agacgaccgc cacgccgtcg atgctctggt gtcagtggct gcggctcaac ctccagatgt gccaaattcc caaggccaca cgtctgtgcc tatcgctgat gggcgtcaac cccaacgacg ggtccgagtt cccgctcggg tgggtgaacc tcaacctcgt cgactacaac ggcaaccttc gcatgggcgt cacctctctc aatctctggc cgggcgaaaa ggccaacccc atcggcacgt gcgtgccgaa ctacaagtcg aagaatccga tcaagctgat cgtggagctc tacaaattcc cacacgtggt caagtacaag cgaccaatcc tcgtcgcccc ggcgtacgag gacaatccct acccgcgagc gccggaacgc aagcgactgg tccacatcat ctcgcaagat tcgctccaca tgcccagcag cgaggagaag aaactgctgt gggagtaccg ccgcacgctg gcgcccaacc ccaaggctct cgccaaggtg ttaatggcca tcaactggtc gcgacccgag gacgtggccg aggcccaccg catgctcacc tactggacgc cgttgtcgcc catcgaagca ctcgagttgc tcgatgccta cttcgccgac gaggtggtgc gcgagtacgc tgtcgccaag ctttgccagc tctcgttgga cgagttgcag gcctatcttc cccagctcac acaggtcctc aaatacgaac cctaccacga ttcagcactg gcgcgtttcc tgttggctgc tgccttccgg catcgaccga cgatcggcca cttctttttc tggcatcttc aggccgagct gcaagtctac ctgcgcggat gcggtcaaca gcgggagggc ctcctcaagc agatggaggt ctcctccaag ttgggccgca tcgccaacct catgaaaccc cttcccttca aggagcgcaa gcaggcgctt caaaacgagc tggtggctgc gggtctgccc tcccacttca ttctgcccat gaacccggcc ttggaggcac gagatctgaa ctgcgacgag tgcaagaccc tcgagtcctt caccgcgccg ctgttcctgt cgtttgacaa caacgacccg gcgggcagcc cgttcaaggt catcttcaag gcgggcgacg atctgcgcca ggacatcctc atcctgcaga tgttcgggat catggatcgg ttgtggaaga agcacgggct ggacctgcac atgaccctct acggatgcac cgccaccggc gaggcggccg ggttcatcga ggtgaaagct ggcggtgcgt cggcggtgtt caaggactcg gtgctcacca actggctcaa gaaatacaac ccacaagagg accagtgggc gggcgtggag gacaacttca tccgatcgtg cgccggctac tgcgtggcca cctatgtgct gggtatcggg gatcgtcaca acgacaacat catggtgacg tacagcgggc acctgttcca catcgacttt gcccacttcc tgggcaacat gatggagtgg aagggcttca agcgagaaac cgctcccttc gtgttcacgc cgcagttcct gcacgtgatg ggcggagaga agggcgccgg gttccagcgc ttcgtgtcgc tctcgtgcct ggcctacaac atcatccgcc gctacgccca catgctgtcg acgggcatca aggagttgaa gggggtggag gatctgcact acttgcgcga ggtgctggcg ttgaacatgt ccgacgagaa ggcggccgag tacttcacca ccaagatcta cgagtcgctc aagaccaagc gcacccagtt caacgacgcc atccacatcc tcgccaacaa caagaagaac gagtga
Details for AccaA.20413.a.B2.GE43865
HARVESTED ON: 11/2/2020
SEQUENCED ON: 12/2/2020
EXPECTED MW: 93kDa
OBSERVED MW: 93kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 29
Validated AA Sequence
MFGIMDRLWK KHGLDLHMTL YGCTATGEAA GFIEVVPDCE TTASIQKKAG GASAVFKDSV LTNWLKKYNP QEDQWAGVED NFIRSCAGYC VATYVLGIGD RHNDNIMVTY SGHLFHIDFA HFLGNMMEWK GFKRETAPFV FTPQFLHVMG GEKGAGFQRF VSLSCLAYNI IRRYAHVIMI LFQLMLSTGI KELKGVEDLH YLREVLALNM SDEKAAEYFT TKIYESLKTK RTQFNDAIHI LAN
Validated NT Sequence
ttcgggcttt gttagcagcc ggatcctatc ttactcactt agttggcgag gatgtggatg gcatcgttga actgggtgcg cttggtcttg agtgactcgt agatcttggt ggtgaagtac tcggccgcct tctcgtctga catgttcaac gccagcacct cgcgcaagta gtgtagatcc tccaccccct tcaactcctt gatgcccgtc gacagcatga gctggaagag gatcatgatg acgtgggcgt agcggcggat gatgttgtat gccaggcacg agagcgacac gaagcgctgg aacccggcgc ccttctctcc gcccatcacg tgcaggaact gcggcgtgaa cacgaaggga gcggtttctc gcttgaagcc cttccactcc atcatgttgc ccagaaagtg ggcaaagtcg atgtggaaca ggtgcccgct gtacgtcacc atgatgttgt cgttgtgacg atccccgata cccagcacgt aggtggccac gcagtagccg gcgcacgatc ggatgaagtt gtcctccacg ccggcccact ggtcctcttg cgggttgtat ttcttgagcc agttggtgag caccgagtcc ttgaacaccg ccgacgcacc gccagctttc ttttgaatgg aggccgtcgt ctcacagtcc ggcaccacct cgatgaaccc agccgcctcg ccggtggccg tgcatccgta gagggtcatg tgcaggtcca gtccgtgctt cttccacaac cgatccataa tcccgaacat ctgcaggatg angatgtcct ggngcngtcg tcgcccgcct tgaagatgac cttgaacggg ctgcccgccg ggtcgttgtt gtcaaacgac aggaacagcg gcgcggtgaa ggattcgaga gtcttgcact cgtcgcantt cagatctcgt gcctccnnnn ngggtncatg ggcagaatgn agtgagangg cagacccgca ncncngctcn ttttgaagcg ncngntncgc tccnnnaagg gaagggntna tgnnnnnnat gcgcactnnn ganactncat ctgctnngna ngccntnccc nnnnnngact
Expected Protein Sequence
MAHHHHHHIQ QVRVKLHFPS GSLVRTLLCS ADDTVYDLVA TGLSSVKIRQ AMSISGEVGD YLVKICGYSW YILNLDTKLR SVDYVRESIK RNKEVEFVLV ERSSFKRTDL QELVYLDHFQ DVDLGMDTEP FDDDEDPSLA GGFGEKKHIP LSEIKRNFEL RVICLEDADF GEVPAMQQLP KGVPVHFYVS AALYHGGESL APEMKTTATP SMLWCQWLRL NLQMCQIPKA TRLCLSLMGV NPNDGSEFPL GWVNLNLVDY NGNLRMGVTS LNLWPGEKAN PIGTCVPNYK SKNPIKLIVE LYKFPHVVKY KRPILVAPAY EDNPYPRAPE RKRLVHIISQ DSLHMPSSEE KKLLWEYRRT LAPNPKALAK VLMAINWSRP EDVAEAHRML TYWTPLSPIE ALELLDAYFA DEVVREYAVA KLCQLSLDEL QAYLPQLTQV LKYEPYHDSA LARFLLAAAF RHRPTIGHFF FWHLQAELQV YLRGCGQQRE GLLKQMEVSS KLGRIANLMK PLPFKERKQA LQNELVAAGL PSHFILPMNP ALEARDLNCD ECKTLESFTA PLFLSFDNND PAGSPFKVIF KAGDDLRQDI LILQMFGIMD RLWKKHGLDL HMTLYGCTAT GEAAGFIEVK AGGASAVFKD SVLTNWLKKY NPQEDQWAGV EDNFIRSCAG YCVATYVLGI GDRHNDNIMV TYSGHLFHID FAHFLGNMME WKGFKRETAP FVFTPQFLHV MGGEKGAGFQ RFVSLSCLAY NIIRRYAHML STGIKELKGV EDLHYLREVL ALNMSDEKAA EYFTTKIYES LKTKRTQFND AIHILAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatccagc aggtgcgggt gaagctccac ttcccctcgg gctcgctcgt gcgcaccctg ctctgctccg ccgacgacac cgtctatgat ttggtggcga ctggtctgtc gagcgtcaag atccgacagg cgatgtccat ctcgggcgaa gtcggcgact acctggtcaa gatctgcgga tactcgtggt acattctcaa cctcgacacc aagctgcgga gcgtggacta cgtgcgcgag agcatcaagc gcaacaagga ggtcgagttc gtcctggtcg agcggtcctc cttcaaacga accgatctcc aggaactggt ctacctcgac cacttccagg acgtcgattt ggggatggac accgagccgt tcgatgacga cgaagatccg tccctcgccg gcggattcgg tgagaagaaa cacatcccgc tctctgaaat caagcgcaac tttgagctta gggtcatttg cctggaggat gcggactttg gcgaggtgcc agcgatgcag cagctcccca agggcgtgcc cgtccacttc tacgtctctg ctgctctcta ccacggcggc gagagtttgg cgccggagat gaagacgacc gccacgccgt cgatgctctg gtgtcagtgg ctgcggctca acctccagat gtgccaaatt cccaaggcca cacgtctgtg cctatcgctg atgggcgtca accccaacga cgggtccgag ttcccgctcg ggtgggtgaa cctcaacctc gtcgactaca acggcaacct tcgcatgggc gtcacctctc tcaatctctg gccgggcgaa aaggccaacc ccatcggcac gtgcgtgccg aactacaagt cgaagaatcc gatcaagctg atcgtggagc tctacaaatt cccacacgtg gtcaagtaca agcgaccaat cctcgtcgcc ccggcgtacg aggacaatcc ctacccgcga gcgccggaac gcaagcgact ggtccacatc atctcgcaag attcgctcca catgcccagc agcgaggaga agaaactgct gtgggagtac cgccgcacgc tggcgcccaa ccccaaggct ctcgccaagg tgttaatggc catcaactgg tcgcgacccg aggacgtggc cgaggcccac cgcatgctca cctactggac gccgttgtcg cccatcgaag cactcgagtt gctcgatgcc tacttcgccg acgaggtggt gcgcgagtac gctgtcgcca agctttgcca gctctcgttg gacgagttgc aggcctatct tccccagctc acacaggtcc tcaaatacga accctaccac gattcagcac tggcgcgttt cctgttggct gctgccttcc ggcatcgacc gacgatcggc cacttctttt tctggcatct tcaggccgag ctgcaagtct acctgcgcgg atgcggtcaa cagcgggagg gcctcctcaa gcagatggag gtctcctcca agttgggccg catcgccaac ctcatgaaac cccttccctt caaggagcgc aagcaggcgc ttcaaaacga gctggtggct gcgggtctgc cctcccactt cattctgccc atgaacccgg ccttggaggc acgagatctg aactgcgacg agtgcaagac cctcgagtcc ttcaccgcgc cgctgttcct gtcgtttgac aacaacgacc cggcgggcag cccgttcaag gtcatcttca aggcgggcga cgatctgcgc caggacatcc tcatcctgca gatgttcggg atcatggatc ggttgtggaa gaagcacggg ctggacctgc acatgaccct ctacggatgc accgccaccg gcgaggcggc cgggttcatc gaggtgaaag ctggcggtgc gtcggcggtg ttcaaggact cggtgctcac caactggctc aagaaataca acccacaaga ggaccagtgg gcgggcgtgg aggacaactt catccgatcg tgcgccggct actgcgtggc cacctatgtg ctgggtatcg gggatcgtca caacgacaac atcatggtga cgtacagcgg gcacctgttc cacatcgact ttgcccactt cctgggcaac atgatggagt ggaagggctt caagcgagaa accgctccct tcgtgttcac gccgcagttc ctgcacgtga tgggcggaga gaagggcgcc gggttccagc gcttcgtgtc gctctcgtgc ctggcctaca acatcatccg ccgctacgcc cacatgctgt cgacgggcat caaggagttg aagggggtgg aggatctgca ctacttgcgc gaggtgctgg cgttgaacat gtccgacgag aaggcggccg agtacttcac caccaagatc tacgagtcgc tcaagaccaa gcgcacccag ttcaacgacg ccatccacat cctcgccaac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat