AccaA.20407.a

protein arginine N-methyltransferase 5 (PRMT5)

CENTER ID: AccaA.20407.a
ORGANISM: Acanthamoeba castellanii
ASSOCIATED DISEASE: Amoebic keratitis and encephalomyelitis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
AccaA.20407.a.B1.GE43864 full length 1 523
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:ACA1_263190
UniProt: L8H144
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDRISLGVD TNCVPDIEET LQFSRSSGRP FDALVIKRDE PLTRSDTLLG SNDWTTSVIG KISPWLDCDS ESAAIRTRSE KAFKQEVAWA THLGLHAVML PAPRFHSTNY SHVINHVASN LSYMQAWVKI PLVAPESEQE ARETDPWEWW NNLRLLAEHN SNMGVALELT ADLPGEKALK RWLGEPVKAV ILPTSIFLTN KLGYPTLSKK HQAFLCRLFR YRLQFIVTGS PHHKDGLSAY QQYIRFLHRK QSPLTDQEHF EAPYWDYLQA PLQPLMDNLE SQTYETFERD PVKYREYERA CFEALKKRGK DEETVLMVVG AGRGPLVAGA LRAAKQAERK LKVYAVDKNP NAVITLRNRK VAEGWDNVTV VDTDMRVWNA PEKADILVSE LLGSFGDNEL SPECLDGAQK FLKEIGGISI PSAYTSYLAP LSSSKLYNEV KAYNDRKHFE TAYVVKFHNT DQLAEAKPCF TFTHPNRAAL IDNSRYTKLS WVIEEASTLH GFGGYFDATL FEEGRSYWIG LQT
NT Sequence
atggacgatc gcatctcgct tggtgttgac accaattgcg tgcccgatat agaggagacg ctccaatttt cgcgttcctc tgggaggccc ttcgacgccc tcgtgatcaa gcgcgatgag ccgctcacgc gctctgacac cctgctcggc tcgaacgact ggacgaccag cgtcatcgga aagatttcgc cctggctcga ttgtgattct gagagtgctg cgatcaggac ccgctccgag aaggccttca agcaggaggt ggcgtgggcg acccatttgg gcctgcacgc tgtaatgctg cccgcacccc gcttccactc gaccaactac tcgcacgtca tcaaccacgt cgcaagcaac ctctcgtaca tgcaggcctg ggtcaagatt ccacttgttg cgccagagtc agagcaagaa gcacgagaga ccgatccttg ggagtggtgg aacaacctcc gcctcttggc tgagcacaac tccaacatgg gcgtagccct ggagctgact gcggacttgc ccggcgagaa ggcgctgaag aggtggctcg gcgagcccgt caaggccgtc attctgccca cgagcatctt cctgacgaac aagttgggtt accccacgct ttccaagaag caccaggcct tcctctgccg tttgttcagg tatcgtctgc aattcatcgt gaccggatcg ccgcaccaca aggacggact ctcggcttac cagcagtaca tccgcttcct gcaccgcaag cagtcgcccc tcaccgacca ggagcacttc gaggcgccct actgggacta cctccaggct ccgctccagc ccctgatgga caacctggag tcacagacgt atgagacctt cgagagggat cccgtcaagt accgcgaata tgagcgggcc tgctttgaag ctttgaagaa gaggggcaaa gatgaggaga ccgtcctcat ggtggttggc gccggccgag gccctctggt tgccggggct ctccgcgccg ctaaacaggc cgagcgaaag ctgaaggtgt acgccgtcga caagaacccc aacgctgtca tcacccttcg caaccgcaag gtcgccgagg gctgggacaa tgtgaccgtg gtcgacaccg acatgagggt gtggaacgcc ccagagaagg ccgatatcct cgtcagcgag ctccttggtt ccttcggtga caacgagctg tcgcccgagt gtctggatgg cgcccagaag ttcctcaagg aaatcggtgg catcagtatt ccgagcgcgt acacgtccta tttggctccg ctcagttcgt ccaagctcta caatgaagtg aaggcgtaca acgaccggaa gcactttgag acggcctatg tcgtcaagtt ccacaacacc gaccagttgg ctgaggccaa gccttgcttc accttcacgc atcccaacag ggctgcgctg atcgacaaca gccgctacac caagctctcg tgggtcatcg aagaggcctc taccctgcac ggttttggcg gttactttga tgccacgctg ttcgaagaag gtcggtccta ctggattggc ctccagacct aa
Details for AccaA.20407.a.B1.GE43864
HARVESTED ON: 11/2/2020
SEQUENCED ON: 12/2/2020
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 21
PERCENT COVERAGE: 59
Validated AA Sequence
MSLHGGSLGH VDGRAQREEE REPTDHALGE SGGVNAEVDF FEQRGIEVTA KTVQGRGLFD DPRELGVSAV VDQRSLVGMR EGEARLGLSQ LVGVVELDNI GRLKVLPVVV RRHFIVELGR TERSQIGRVR ARNTDATDFL EELLGAIQTL GRQLVVTEGA KELADEDVGL LRGVPHPHVG VDHGHIVPAL SDLAVAKGDD SVGVLVDGVH LQLSLGLFGS SESPGNQRAS AGANNHEDGL LIFAPLLQSF KAGPLIFAVL DGIPLEGLVR LRLQVVHXXW XXPXXSXXXX SXXXXRXXXC XXXXVLLXXX XRXXXX
Validated NT Sequence
cnttcgggct ttgttagcag ccggatccta tcttactcac ttaggtctgg aggccaatcc agtaggaccg accttgcggg ttgtggacgg gcgagattga cggagaggtg acagtccact cataccacac cttggtggag gtgacgtgcc gccagaaatg agcctccacg gtggctccct tgggcacgta gatgggcgtg cgcagaggga agaagagagg gaaccaactg atcatgccct cggagaaagt ggcggggtta atgctgaggt ggacttcttc gaacagcgtg gcatcgaagt aaccgccaaa accgtgcagg gtagaggcct cttcgatgac ccacgagagc ttggtgtatc ggctgttgtc gatcagcgca gccttgttgg gatgcgtgaa ggtgaagcaa ggcttggcct cagccaactg gtcggtgttg tggaacttga caacataggc cgtctcaaag tgcttccggt cgttgtacgc cgtcacttca ttgtagagct tggacgaact gagcggagcc aaataggacg tgtacgcgct cggaatactg atgccaccga tttccttgag gaacttctgg gcgccatcca gacactcggg cgacagctcg ttgtcaccga aggagccaag gagctcgctg acgaggatgt cggccttctc cggggcgttc cacaccctca tgtcggtgtc gaccacggtc acattgtccc agccctcagc gaccttgcgg ttgcgaaggg tgatgacagc gttggggttc ttgtcgacgg cgtacacctt cagctttcgc tcggcctgtt tggcagctcg gagagccccg gcaaccagag ggcctcggcc ggcgccaaca accatgagga cggtctcctc atctttgccc ctcttcttca aagcttcaaa gcaggcccgc tcatattcgc ggtacttgac gggatccctc tcgaaggtct cgtacgtctg cgactccagg ttgtccatcn nnnctgganc gnncctnnnn agtccngtnn ncncnnnagt gntnctgngn ngagggnnna ctgntgcgnn cngnncgntg tactgctgnn gnnnanantc cgtnnttnnn gggnnnng
Expected Protein Sequence
MAHHHHHHMD DRISLGVDTN CVPDIEETLQ FSRSSGRPFD ALVIKRDEPL TRSDTLLGSN DWTTSVIGKI SPWLDCDSES AAIRTRSEKA FKQEVAWATH LGLHAVMLPA PRFHSTNYSH VINHVASNLS YMQAWVKIPL VAPESEQEAR ETDPWEWWNN LRLLAEHNSN MGVALELTAD LPGEKALKRW LGEPVKAVIL PTSIFLTNKL GYPTLSKKHQ AFLCRLFRYR LQFIVTGSPH HKDGLSAYQQ YIRFLHRKQS PLTDQEHFEA PYWDYLQAPL QPLMDNLESQ TYETFERDPV KYREYERACF EALKKRGKDE ETVLMVVGAG RGPLVAGALR AAKQAERKLK VYAVDKNPNA VITLRNRKVA EGWDNVTVVD TDMRVWNAPE KADILVSELL GSFGDNELSP ECLDGAQKFL KEIGGISIPS AYTSYLAPLS SSKLYNEVKA YNDRKHFETA YVVKFHNTDQ LAEAKPCFTF THPNRAALID NSRYTKLSWV IEEASTLHGF GGYFDATLFE EGRSYWIGLQ T
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg atcgcatctc gcttggtgtt gacaccaatt gcgtgcccga tatagaggag acgctccaat tttcgcgttc ctctgggagg cccttcgacg ccctcgtgat caagcgcgat gagccgctca cgcgctctga caccctgctc ggctcgaacg actggacgac cagcgtcatc ggaaagattt cgccctggct cgattgtgat tctgagagtg ctgcgatcag gacccgctcc gagaaggcct tcaagcagga ggtggcgtgg gcgacccatt tgggcctgca cgctgtaatg ctgcccgcac cccgcttcca ctcgaccaac tactcgcacg tcatcaacca cgtcgcaagc aacctctcgt acatgcaggc ctgggtcaag attccacttg ttgcgccaga gtcagagcaa gaagcacgag agaccgatcc ttgggagtgg tggaacaacc tccgcctctt ggctgagcac aactccaaca tgggcgtagc cctggagctg actgcggact tgcccggcga gaaggcgctg aagaggtggc tcggcgagcc cgtcaaggcc gtcattctgc ccacgagcat cttcctgacg aacaagttgg gttaccccac gctttccaag aagcaccagg ccttcctctg ccgtttgttc aggtatcgtc tgcaattcat cgtgaccgga tcgccgcacc acaaggacgg actctcggct taccagcagt acatccgctt cctgcaccgc aagcagtcgc ccctcaccga ccaggagcac ttcgaggcgc cctactggga ctacctccag gctccgctcc agcccctgat ggacaacctg gagtcacaga cgtatgagac cttcgagagg gatcccgtca agtaccgcga atatgagcgg gcctgctttg aagctttgaa gaagaggggc aaagatgagg agaccgtcct catggtggtt ggcgccggcc gaggccctct ggttgccggg gctctccgcg ccgctaaaca ggccgagcga aagctgaagg tgtacgccgt cgacaagaac cccaacgctg tcatcaccct tcgcaaccgc aaggtcgccg agggctggga caatgtgacc gtggtcgaca ccgacatgag ggtgtggaac gccccagaga aggccgatat cctcgtcagc gagctccttg gttccttcgg tgacaacgag ctgtcgcccg agtgtctgga tggcgcccag aagttcctca aggaaatcgg tggcatcagt attccgagcg cgtacacgtc ctatttggct ccgctcagtt cgtccaagct ctacaatgaa gtgaaggcgt acaacgaccg gaagcacttt gagacggcct atgtcgtcaa gttccacaac accgaccagt tggctgaggc caagccttgc ttcaccttca cgcatcccaa cagggctgcg ctgatcgaca acagccgcta caccaagctc tcgtgggtca tcgaagaggc ctctaccctg cacggttttg gcggttactt tgatgccacg ctgttcgaag aaggtcggtc ctactggatt ggcctccaga cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat